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Among sulfate-reducing bacteria, degradation of acetone and other ketones is still an uncommon ability and has not been understood completely yet. In the present work, we show that <jats:italic>Desulfotomaculum arcticum<\/jats:italic> and <jats:italic>Desulfotomaculum geothermicum<\/jats:italic> are able to degrade acetone and butanone. Total proteomics of cell-free extracts of both organisms indicated an involvement of a thiamine diphosphate-dependent enzyme, a B<jats:sub>12<\/jats:sub>-dependent mutase, and a specific dehydrogenase during acetone degradation. Similar enzymes were recently described to be involved in acetone degradation by <jats:italic>Desulfococcus biacutus<\/jats:italic>. As there are so far only two described sulfate reducers able to degrade acetone, <jats:italic>D. arcticum<\/jats:italic> and <jats:italic>D. geothermicum<\/jats:italic> represent two further species with this capacity. All these bacteria appear to degrade acetone via the same set of enzymes and therefore via the same pathway.<\/jats:p>","DOI":"10.1007\/s00284-021-02441-9","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T07:02:56Z","timestamp":1616396576000},"page":"1763-1770","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Two Marine Desulfotomaculum spp. of Different Origin are Capable of Utilizing Acetone and Higher Ketones"],"prefix":"10.1007","volume":"78","author":[{"given":"Jasmin","family":"Frey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Ka\u00dfner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"Schink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,3,22]]},"reference":[{"issue":"3","key":"2441_CR1","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1007\/s00253-011-3276-5","volume":"91","author":"B Han","year":"2011","unstructured":"Han B, Gopalan V, Ezeji TC (2011) Acetone production in solventogenic Clostridium species: new insights from non-enzymatic decarboxylation of acetoacetate. 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