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The most common manifestation is cryptococcal meningitis in AIDS patients. Diagnosis primarily relies on antigen testing from serum and cerebrospinal fluid (CSF). Current guidelines recommend rapid antigen testing with a focus on point-of-care assays. Over the recent years, a range of new lateral flow assays (LFAs) was launched. There is still a lack of data evaluating the CE-certified Biosynex RDT CryptoPS LFA. We compared the performance of this LFA with a latex agglutination assay (LAA; Latex-Cryptococcus Antigen Detection System, IMMY) from blood and CSF samples. Blood and\/or CSF samples of 27 patients with proven cryptococcal infections caused by different species and blood\u2013CSF pairs of 20 controls were tested applying LFA and LAA. Upon combined analysis of blood and CSF, both assays were able to identify all <jats:italic>C.<\/jats:italic>\u00a0<jats:italic>neoformans<\/jats:italic> infections. Based on CSF analysis only, the LFA and the LAA had sensitivities of 100% and 93%. Neither test gave false-positive results nor was reactive in two cases of <jats:italic>C.<\/jats:italic>\u00a0non-<jats:italic>neoformans<\/jats:italic>\/non-<jats:italic>gattii<\/jats:italic> species infections. Both assays have high sensitivities and specificities for the diagnosis of <jats:italic>C.<\/jats:italic>\u00a0<jats:italic>neoformans<\/jats:italic> infection. Contrarily to the IMMY LAA, the RDT CryptoPS LFA is suitable as a point-of-care test but is limited in the quantification of antigen reactivity.<\/jats:p>","DOI":"10.1007\/s00284-021-02664-w","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T06:39:12Z","timestamp":1632811152000},"page":"3989-3995","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Comparison of a Lateral Flow Assay and a Latex Agglutination Test for the Diagnosis of Cryptococcus Neoformans Infection"],"prefix":"10.1007","volume":"78","author":[{"given":"Thilo","family":"Schub","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Forster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Suerbaum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Wagener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5032-8870","authenticated-orcid":false,"given":"Karl","family":"Dichtl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,28]]},"reference":[{"key":"2664_CR1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00449-20","author":"MC Fisher","year":"2020","unstructured":"Fisher MC, Gurr SJ, Cuomo CA, Blehert DS, Jin H, Stukenbrock EH, Stajich JE, Kahmann R, Boone C, Denning DW, Gow NAR, Klein BS, Kronstad JW, Sheppard DC, Taylor JW, Wright GD, Heitman J, Casadevall A, Cowen LE (2020) Threats posed by the fungal kingdom to humans, wildlife, and agriculture. 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Johannes Forster, Johannes Wagener, and Karl Dichtl report grants and non-financial support from Fujifilm Wako Europe. JW and KD report grants and non-financial support from Euroimmun Labordiagnostika. JW reports personal fees from Pfizer. All grants, non-financial support, and fees are outside the submitted work.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The authors confirm that the ethical policies of the journal, as noted on the journal\u2019s author guidelines page, have been adhered to. The appropriate ethical review committee approvals have been received at the respective study sites (Ethikkommission bei der Medizinischen Fakult\u00e4t der LMU M\u00fcnchen: 17\u2013565; Ethik-Kommission der Universit\u00e4t W\u00fcrzburg: 20181128\u00a002).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"A waiver of informed consent was granted.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed Consent"}}]}}