{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:15:56Z","timestamp":1787760956425,"version":"build-2784847793"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T00:00:00Z","timestamp":1641254400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T00:00:00Z","timestamp":1641254400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the Central Public-interest Scientific Institution Basal Research Fund","award":["CAFS(2020TD78)"],"award-info":[{"award-number":["CAFS(2020TD78)"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Curr Microbiol"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The study aimed to evaluate the safety of copper ion sterilization based on copper ion residues in zebrafish (<jats:italic>Brachydanio rerio<\/jats:italic>), as well as bacterial community structure and diversity in recirculating aquaculture systems (RASs). The copper ion content was determined using national food safety standard GB 5009.13-2017. Bacterial community structures and alpha and beta diversity indexes were examined using the 16S rRNA gene sequences produced by Illumina HiSeq sequencing. The results revealed no significant copper ion enrichment in <jats:italic>B. rerio<\/jats:italic> when the copper ion concentration was 0.15\u00a0mg\/L. The relative abundances of <jats:italic>Erythrobacter<\/jats:italic>, nitrite bacteria, and <jats:italic>Flavanobacteria<\/jats:italic> were clearly higher in the treatment group than in the control and differences in bacterial species richness and diversity were obvious. In addition, there was no sharp decrease in the microflora at the outflow of the copper ion generator. In conjunction with the changes in ammonia nitrogen, nitrate, and nitrite concentrations during the experiment, the results indicated that there were no significant effects on the purification efficacy of the biological filter, but the abundances of beneficial bacteria increased significantly. This is of great relevance in order to understand the response of bacterial communities affected by changing environmental conditions, such as copper ion sterilization.<\/jats:p>","DOI":"10.1007\/s00284-021-02707-2","type":"journal-article","created":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T10:08:03Z","timestamp":1641290883000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of Copper Ion Sterilization on Bacterial Community in a Freshwater Recirculating Aquaculture System"],"prefix":"10.1007","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8162-2922","authenticated-orcid":false,"given":"Jianjun","family":"Shan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1375-2514","authenticated-orcid":false,"given":"Xiaoqing","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6056-7226","authenticated-orcid":false,"given":"Chongwu","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5853-6524","authenticated-orcid":false,"given":"Chenglin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,4]]},"reference":[{"key":"2707_CR1","doi-asserted-by":"publisher","first-page":"269","DOI":"10.3969\/j.issn.1673-1921.2011.05.263","volume":"5","author":"WT Tian","year":"2011","unstructured":"Tian WT (2011) Application of modern biotechnology in aquaculture. Modern Anim Husband Sci Technol 5:269. https:\/\/doi.org\/10.3969\/j.issn.1673-1921.2011.05.263","journal-title":"Modern Anim Husband Sci Technol"},{"key":"2707_CR2","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.marpol.2019.02.045","volume":"104","author":"J Guillen","year":"2019","unstructured":"Guillen J, Asche F, Carvalho N, Fernandez Polanco JM, Llorente I, Nielsen R, Nielsen M, Villasante S (2019) Aquaculture subsidies in the european union: evolution, impact and future potential for growth. Mar Policy 104:19\u201328. https:\/\/doi.org\/10.1016\/j.marpol.2019.02.045","journal-title":"Mar Policy"},{"issue":"03","key":"2707_CR3","doi-asserted-by":"publisher","first-page":"70","DOI":"10.13721\/j.cnki.dsyy.20150309.005","volume":"45","author":"YT Tang","year":"2015","unstructured":"Tang YT, Zhao LJ, Yang Y, Liu QG, Fan YC, Wu JY (2015) Effects of Hyriopsis cumingii, Hypophthalmichthys molitrix and Aristichthys nobilis polycultured on microbial community structure in aquaculture ponds \/enclosures: a comparative study-Based on 16S rDNA sequences. Freshw Fish 45(03):70\u201377. https:\/\/doi.org\/10.13721\/j.cnki.dsyy.20150309.005","journal-title":"Freshw Fish"},{"key":"2707_CR4","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S0882-4010(95)90076-4","volume":"59","author":"RI Amann","year":"1995","unstructured":"Amann RI (1995) Phylogenetic identifieation and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143\u2013169. https:\/\/doi.org\/10.1016\/S0882-4010(95)90076-4","journal-title":"Microbiol Rev"},{"issue":"8","key":"2707_CR5","doi-asserted-by":"publisher","first-page":"4449","DOI":"10.1111\/are.13270","volume":"48","author":"T Zhou","year":"2017","unstructured":"Zhou T, Zheng XF, Tang JY, Qin JG, Wang Y (2017) Effect of three commercial microbial products on bacterial community in a freshwater fish polyculture system. Aquac Res 48(8):4449\u20134460","journal-title":"Aquac Res"},{"key":"2707_CR6","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1007\/s10499-006-9051-4","volume":"14","author":"E Mente","year":"2006","unstructured":"Mente E, Pierce GJ, Bego\u00f1a Santos M, Neofitou C (2006) Effect of feed and feeding in the culture of salmonids on the marine aquatic environment: a synthesis for European aquaculture. Aquacult Int 14:499\u2013522","journal-title":"Aquacult Int"},{"issue":"9","key":"2707_CR7","first-page":"138","volume":"35","author":"JJ Shan","year":"2019","unstructured":"Shan JJ, Guan CW, Zhang CL, Zhang YL, Wu F (2019) The Concentration of Cu2+: effects on germicidal efficacy in recirculating aquaculture system. Chin Agric Sci Bull 35(9):138\u2013142","journal-title":"Chin Agric Sci Bull"},{"key":"2707_CR8","first-page":"106","volume":"01","author":"L Liu","year":"2017","unstructured":"Liu L (2017) Application of beneficial microorganisms in aquaculture. Jiangxi Agic 01:106\u2013107","journal-title":"Jiangxi Agic"},{"issue":"86 & 04","key":"2707_CR9","doi-asserted-by":"publisher","first-page":"86","DOI":"10.14184\/j.cnki.issn1004-843x.2015.03.054","volume":"03","author":"X Yuan","year":"2015","unstructured":"Yuan X, Fan QH (2015) Application of beneficial microorganisms in aquaculture. Sci Fish Farm 03(86 & 04):86. https:\/\/doi.org\/10.14184\/j.cnki.issn1004-843x.2015.03.054","journal-title":"Sci Fish Farm"},{"key":"2707_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.137812","volume":"722","author":"ZC Lang","year":"2020","unstructured":"Lang ZC, Zhou MH, Zhang QZ, Yin XY, Li YW (2020) Comprehensive treatment of marine aquaculture wastewater by a cost-effective flow-through electro-oxidation process. Sci Total Environ 722:137812. https:\/\/doi.org\/10.1016\/j.scitotenv.2020.137812","journal-title":"Sci Total Environ"},{"issue":"01","key":"2707_CR11","doi-asserted-by":"publisher","first-page":"48","DOI":"10.13679\/j.advps.2016.1.00048","volume":"27","author":"LD Yuan","year":"2016","unstructured":"Yuan LD, Li LZ, Tian XQ, Tang YY, Fan CQ, Huang HL, Yang Q (2016) Biodiversity and phylogenetic analysis of the gut microbiome of Euphausia superba Dana from the Rose Sea of the Antarctic Ocean. Adv Polar Sci 27(01):48\u201355. https:\/\/doi.org\/10.13679\/j.advps.2016.1.00048","journal-title":"Adv Polar Sci"},{"key":"2707_CR12","doi-asserted-by":"publisher","first-page":"2360","DOI":"10.1038\/ismej.2014.84","volume":"8","author":"P De Schryver","year":"2014","unstructured":"De Schryver P, Vadstein O (2014) Ecological theory as a foundation to control pathogenic invasion in aquaculture. Int Soc Microb Ecol 8:2360\u20132368. https:\/\/doi.org\/10.1038\/ismej.2014.84","journal-title":"Int Soc Microb Ecol"},{"key":"2707_CR13","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00101","author":"RP Bartelme","year":"2017","unstructured":"Bartelme RP, McLellan SL, Newton RJ (2017) Freshwater recirculating aquaculture system operations drive biofilter bacterial community shifts around a stable nitrifying consortium of ammonia-oxidizing archaea and Comammox Nitrospira. Front Microbiol. https:\/\/doi.org\/10.3389\/fmicb.2017.00101","journal-title":"Front Microbiol"},{"key":"2707_CR14","doi-asserted-by":"publisher","first-page":"1483","DOI":"10.1038\/ismej.2016.198","volume":"11","author":"M Simon","year":"2017","unstructured":"Simon M, Scheuner C, Meier-Kolthoff JP, Brinkhoff T, Wagner-D\u00f6bler I, Ulbrich M, Klenk HP, Schomburg D, Petersen J, G\u00f6ker M (2017) Phylogenomics of Rhodobacteraceae reveals evolutionary adaptation to marine and non-marine habitats. Int Soc Microb Ecol 11:1483\u20131499. https:\/\/doi.org\/10.1038\/ismej.2016.198","journal-title":"Int Soc Microb Ecol"},{"issue":"01","key":"2707_CR15","doi-asserted-by":"publisher","first-page":"231","DOI":"10.13227\/j.hjkx.2011.01.031","volume":"32","author":"HG Zhang","year":"2011","unstructured":"Zhang HG, Ma SS, Li QF, Fu XJ, Zhang Y, Qu KM (2011) Analysis of the changes of microbial community structure on bio-carrier of Recirculating Aquaculture Systems (RAS). Environ Sci 32(01):231\u2013239. https:\/\/doi.org\/10.13227\/j.hjkx.2011.01.031","journal-title":"Environ Sci"},{"key":"2707_CR16","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/s0168-1605(99)00162-2","volume":"53","author":"PJ Jooste","year":"1999","unstructured":"Jooste PJ, CeliaJ H (1999) The taxonomy, ecology and cultivation of bacterial genera belonging to the family flavobacteriaceae. Int J Food Microbiol 53:81\u201394. https:\/\/doi.org\/10.1016\/s0168-1605(99)00162-2","journal-title":"Int J Food Microbiol"},{"issue":"5","key":"2707_CR17","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1111\/jfd.13156","volume":"43","author":"R Avendao-Herrera","year":"2020","unstructured":"Avendao-Herrera R, Benavides I, Espina JA, Soto-Comte D, Poblete-Morales M, Vald\u00e9s JA, Feij\u00f3o CG, Reyes AE (2020) Zebrafish (danio rerio) as an animal model for bath infection by flavobacterium psychrophilum. J Fish Dis 43(5):561\u2013570. https:\/\/doi.org\/10.1111\/jfd.13156","journal-title":"J Fish Dis"},{"issue":"1","key":"2707_CR18","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1099\/00207713-44-1-83","volume":"44","author":"S Orso","year":"1994","unstructured":"Orso S, Gouy M, Navarro E, Normand P (1994) Molecular phylogenetic analysis of Nitrobacter spp. Int J Syst Bacteriol 44(1):83\u201386. https:\/\/doi.org\/10.1099\/00207713-44-1-83","journal-title":"Int J Syst Bacteriol"},{"issue":"17","key":"2707_CR19","doi-asserted-by":"publisher","first-page":"3869","DOI":"10.1016\/j.biortech.2009.03.021","volume":"100","author":"J Yin","year":"2009","unstructured":"Yin J, Xu WF (2009) Ammonia biofiltration and community analysis of ammonia-oxidizing bacteria in biofilters. Biores Technol 100(17):3869\u20133876. https:\/\/doi.org\/10.1016\/j.biortech.2009.03.021","journal-title":"Biores Technol"},{"issue":"12","key":"2707_CR20","doi-asserted-by":"publisher","first-page":"5368","DOI":"10.1128\/AEM.66.12.5368-5382.2000","volume":"66","author":"U Purkhold","year":"2000","unstructured":"Purkhold U, Pommerening-R\u00f6ser A, Juretschko S, Schmid MC, Koops HP, Wagner M (2000) Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys. Appl Environ Microbiol 66(12):5368\u20135382. https:\/\/doi.org\/10.1128\/AEM.66.12.5368-5382.2000","journal-title":"Appl Environ Microbiol"},{"issue":"05","key":"2707_CR21","doi-asserted-by":"publisher","first-page":"73","DOI":"10.11758\/yykxjz.20160330001","volume":"38","author":"Z Jl","year":"2017","unstructured":"Jl Z, Jiang M, Wang CF, Tong XL, Jin J, Gu DP, Hu WG, Yu ZL, Dai XL (2017) Microbial diversity in bio-filter of the recirculating aquaculture system for Litopenaeus vannamei. Progress Fish Sci 38(05):73\u201382. https:\/\/doi.org\/10.11758\/yykxjz.20160330001","journal-title":"Progress Fish Sci"},{"issue":"1","key":"2707_CR22","doi-asserted-by":"publisher","first-page":"130","DOI":"10.11693\/hyhz20160700176","volume":"48","author":"Q Chen","year":"2017","unstructured":"Chen Q, Li GY, Luo K, Kong J, Mo ZL, Luan S, Li J, Cao BX, Zhang YL (2017) Microbial diversity in broodstock waters of the two genders of Litopenaeus Vannamel. Oceanol Limnol Sin 48(1):130\u2013138. https:\/\/doi.org\/10.11693\/hyhz20160700176","journal-title":"Oceanol Limnol Sin"},{"issue":"07","key":"2707_CR23","doi-asserted-by":"publisher","first-page":"47","DOI":"10.3969\/j.issn.1006-6500.2019.07.010","volume":"25","author":"ZG Guan","year":"2019","unstructured":"Guan ZG, Dou Y, Yu SG, Zhou WL, Shao P, Gao JW, Jia XY (2019) Effects of growth and antioxidase activities of Litopenaeus vannamei through rhizobium adding in the fodder. Tianjin Agric Sci 25(07):47\u201351. https:\/\/doi.org\/10.3969\/j.issn.1006-6500.2019.07.010","journal-title":"Tianjin Agric Sci"},{"key":"2707_CR24","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/462832","author":"NJ Rowan","year":"2011","unstructured":"Rowan NJ (2011) Defining established and emerging microbial risks in the aquatic environment: current knowledge, implications, and outlooks. Int J Microbiol. https:\/\/doi.org\/10.1155\/2011\/462832","journal-title":"Int J Microbiol"},{"issue":"2","key":"2707_CR25","doi-asserted-by":"publisher","first-page":"831","DOI":"10.19674\/j.cnki.issn1000-6923.2019.0102","volume":"39","author":"X Luo","year":"2019","unstructured":"Luo X, Yuan LX, Zhang WL, Zhong WZ, Jiang YF, Zhang Y, Xu DS (2019) Correlation study between resistance genes and microbial communities in pharmaceutical wastewater treatment plants. China Environ Sci 39(2):831\u2013838. https:\/\/doi.org\/10.19674\/j.cnki.issn1000-6923.2019.0102","journal-title":"China Environ Sci"},{"issue":"19","key":"2707_CR26","doi-asserted-by":"publisher","first-page":"5884","DOI":"10.1128\/aem.01763-14","volume":"80","author":"GA Cangelosi","year":"2014","unstructured":"Cangelosi GA, Meschke JS (2014) Dead or Alive: Molecular Assessment of Microbial Viability. Appl Environ Microbiol 80(19):5884\u20135891. https:\/\/doi.org\/10.1128\/aem.01763-14","journal-title":"Appl Environ Microbiol"},{"key":"2707_CR27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.fm.2016.06.013","volume":"60","author":"M del Mu\u00f1oz","year":"2016","unstructured":"del Mu\u00f1oz M, Benomar N, Lerma LL, Knapp CW, G\u00e1lvez A, Abriouel H (2016) Biocide tolerance, phenotypic and molecular response of lactic acid bacteria isolated from naturally-fermented Alore\u00f1a table to different Physico-chemical stresses. Food Microbiol 60:1\u201312","journal-title":"Food Microbiol"}],"container-title":["Current Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-021-02707-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00284-021-02707-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-021-02707-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T10:10:21Z","timestamp":1644228621000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00284-021-02707-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,4]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["2707"],"URL":"https:\/\/doi.org\/10.1007\/s00284-021-02707-2","relation":{},"ISSN":["0343-8651","1432-0991"],"issn-type":[{"value":"0343-8651","type":"print"},{"value":"1432-0991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,4]]},"assertion":[{"value":"11 March 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 November 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 January 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflicts of interest. This study was approved by the Animal Care and Use Committee of the Chinese Academy of Fishery Sciences, Beijing, China. All of the animal procedures were conducted in compliance with the Guidelines for the Care and Use of Laboratory Animals in China.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"58"}}