{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T23:55:52Z","timestamp":1793404552911,"version":"4.5.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Curr Microbiol"],"published-print":{"date-parts":[[2023,1]]},"DOI":"10.1007\/s00284-022-03122-x","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T16:31:33Z","timestamp":1669393893000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Impact of N-Acetylcysteine and Antibiotics Against Single and Dual Species Biofilms of Pseudomonas aeruginosa and Achromobacter xylosoxidans"],"prefix":"10.1007","volume":"80","author":[{"given":"Fatima Nur","family":"Yilmaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0823-631X","authenticated-orcid":false,"given":"Mayram","family":"Hacioglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebru Haciosmanoglu","family":"Aldogan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,25]]},"reference":[{"issue":"1","key":"3122_CR1","doi-asserted-by":"publisher","first-page":"15010","DOI":"10.1038\/nrdp.2015.10","volume":"14","author":"F Ratjen","year":"2015","unstructured":"Ratjen F, Bell SC, Rowe SM, Goss CH, Quittner AL, Bush A (2015) Cystic fibrosis. Nat Rev Dis Primers 14(1):15010. https:\/\/doi.org\/10.1038\/nrdp.2015.10","journal-title":"Nat Rev Dis Primers"},{"issue":"4","key":"3122_CR2","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1111\/apm.12689","volume":"125","author":"N H\u00f8iby","year":"2017","unstructured":"H\u00f8iby N, Bjarnsholt T, Moser C, Jensen P\u00d8, Kolpen M, Qvist T et al (2017) Diagnosis of biofilm infections in cystic fibrosis patients. APMIS 125(4):339\u2013343. https:\/\/doi.org\/10.1111\/apm.12689","journal-title":"APMIS"},{"issue":"9","key":"3122_CR3","doi-asserted-by":"publisher","first-page":"881","DOI":"10.3201\/eid0809.020063","volume":"8","author":"RM Donlan","year":"2002","unstructured":"Donlan RM (2002) Biofilms: microbial life on surfaces. Emerg Infect Dis 8(9):881\u2013890. https:\/\/doi.org\/10.3201\/eid0809.020063","journal-title":"Emerg Infect Dis"},{"issue":"2","key":"3122_CR4","doi-asserted-by":"publisher","first-page":"100031","DOI":"10.1016\/j.bioflm.2020.100031","volume":"13","author":"E Vandeplassche","year":"2020","unstructured":"Vandeplassche E, Sass A, Ostyn L, Burm\u00f8lle M, Kragh KN, Bjarnsholt T et al (2020) Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm. Biofilm 13(2):100031. https:\/\/doi.org\/10.1016\/j.bioflm.2020.100031","journal-title":"Biofilm"},{"key":"3122_CR5","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1093\/femsle\/fnx128","volume":"15","author":"S O'Brien","year":"2017","unstructured":"O\u2019Brien S, Fothergill JL (2017) The role of multispecies social interactions in shaping Pseudomonas aeruginosa pathogenicity in the cystic fibrosis lung. FEMS Microbiol Lett 15:364. https:\/\/doi.org\/10.1093\/femsle\/fnx128","journal-title":"FEMS Microbiol Lett"},{"issue":"9","key":"3122_CR6","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1128\/AAC.00302-17","volume":"61","author":"S Tavernier","year":"2017","unstructured":"Tavernier S, Crabb\u00e9 A, Hacioglu M, Stuer L, Henry S, Rigole P et al (2017) Community composition determines activity of antibiotics against multispecies biofilms. Antimicrob Agents Chemother 61(9):302\u2013317. https:\/\/doi.org\/10.1128\/AAC.00302-17","journal-title":"Antimicrob Agents Chemother"},{"key":"3122_CR7","doi-asserted-by":"publisher","first-page":"558160","DOI":"10.3389\/fmicb.2020.558160","volume":"11","author":"Q Menetrey","year":"2020","unstructured":"Menetrey Q, Dupont C, Chiron R, Jumas-Bilak E, Marchandin H (2020) High occurrence of bacterial competition among clinically documented opportunistic pathogens including Achromobacter xylosoxidans in cystic fibrosis. Front Microbiol 11:558160. https:\/\/doi.org\/10.3389\/fmicb.2020.558160","journal-title":"Front Microbiol"},{"issue":"3","key":"3122_CR8","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1016\/j.jcf.2015.03.012","volume":"14","author":"MD Parkins","year":"2015","unstructured":"Parkins MD, Floto RA (2015) Emerging bacterial pathogens and changing concepts of bacterial pathogenesis in cystic fibrosis. J Cyst Fibros 14(3):293\u2013304. https:\/\/doi.org\/10.1016\/j.jcf.2015.03.012","journal-title":"J Cyst Fibros"},{"issue":"2","key":"3122_CR9","doi-asserted-by":"publisher","first-page":"217","DOI":"10.3390\/ph15020217","volume":"15","author":"M Guerini","year":"2022","unstructured":"Guerini M, Condr\u00f2 G, Friuli V, Maggi L, Perugini P (2022) N-acetylcysteine (NAC) and \u0131ts role in clinical practice management of cystic fibrosis (CF): a review. Pharmaceuticals 15(2):217. https:\/\/doi.org\/10.3390\/ph15020217","journal-title":"Pharmaceuticals"},{"issue":"9","key":"3122_CR10","doi-asserted-by":"publisher","first-page":"2388","DOI":"10.1093\/jac\/dky185","volume":"73","author":"S Pollini","year":"2018","unstructured":"Pollini S, Boncompagni S, Di Maggio T, Di Pilato V, Spanu T, Fiori B et al (2018) In vitro synergism of colistin in combination with N-acetylcysteine against Acinetobacter baumannii grown in planktonic phase and in biofilms. J Antimicrob Chemother 73(9):2388\u20132395. https:\/\/doi.org\/10.1093\/jac\/dky185","journal-title":"J Antimicrob Chemother"},{"issue":"3","key":"3122_CR11","doi-asserted-by":"publisher","first-page":"101","DOI":"10.3390\/antibiotics8030101","volume":"8","author":"N Ciacci","year":"2019","unstructured":"Ciacci N, Boncompagni S, Valzano F, Cariani L, Aliberti S, Blasi F et al (2019) In vitro synergism of colistin and N-acetylcysteine against Stenotrophomonas maltophilia. Antibiotics 8(3):101. https:\/\/doi.org\/10.3390\/antibiotics8030101","journal-title":"Antibiotics"},{"issue":"3","key":"3122_CR12","first-page":"261","volume":"58","author":"MA El-Feky","year":"2009","unstructured":"El-Feky MA, El-Rehewy MS, Hassan MA, Abolella HA, Abd El-Baky RM, Gad GF (2009) Effect of ciprofloxacin and N-acetylcysteine on bacterial adherence and biofilm formation on ureteral stent surfaces. Pol J Microbiol 58(3):261\u2013267","journal-title":"Pol J Microbiol"},{"key":"3122_CR13","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.micpath.2017.02.030","volume":"105","author":"D Eroshenko","year":"2017","unstructured":"Eroshenko D, Polyudova T, Korobov V (2017) N-acetylcysteine inhibits growth, adhesion and biofilm formation of Gram-positive skin pathogens. Microb Pathog 105:145\u2013152. https:\/\/doi.org\/10.1016\/j.micpath.2017.02.030","journal-title":"Microb Pathog"},{"issue":"6","key":"3122_CR14","doi-asserted-by":"publisher","first-page":"2655","DOI":"10.1128\/AAC.00045-11","volume":"55","author":"G Brackman","year":"2011","unstructured":"Brackman G, Cos P, Maes L, Nelis HJ, Coenye T (2011) Quorum sensing inhibitors increase the susceptibility of bacterial biofilms to antibiotics in vitro and in vivo. Antimicrob Agents Chemother 55(6):2655\u20132661. https:\/\/doi.org\/10.1128\/AAC.00045-11","journal-title":"Antimicrob Agents Chemother"},{"issue":"3","key":"3122_CR15","doi-asserted-by":"publisher","first-page":"114863","DOI":"10.1016\/j.diagmicrobio.2019.06.014","volume":"95","author":"M Hacioglu","year":"2019","unstructured":"Hacioglu M, Haciosmanoglu E, Birteksoz-Tan AS, Bozkurt-Guzel C, Savage PB (2019) Effects of ceragenins and conventional antimicrobials on Candida albicans and Staphylococcus aureus mono and multispecies biofilms. Diagn Microbiol Infect Dis 95(3):114863. https:\/\/doi.org\/10.1016\/j.diagmicrobio.2019.06.014","journal-title":"Diagn Microbiol Infect Dis"},{"key":"3122_CR16","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot4493","author":"S Kasibhatla","year":"2006","unstructured":"Kasibhatla S, Amarante-Mendes GP, Finucane D, Brunner T, Bossy-Wetzel E, Green DR (2006) Acridine Orange\/Ethidium Bromide (AO\/EB) staining to detect apoptosis. Cold Spring Harb Protoc. https:\/\/doi.org\/10.1101\/pdb.prot4493","journal-title":"Cold Spring Harb Protoc"},{"key":"3122_CR17","doi-asserted-by":"publisher","first-page":"280","DOI":"10.3389\/fcimb.2017.00280","volume":"7","author":"K Richter","year":"2017","unstructured":"Richter K, Thomas N, Zhang G et al (2017) Deferiprone and gallium-protoporphyrin have the capacity to potentiate the activity of antibiotics in Staphylococcus aureus small colony variants. Front Cell Infect Microbiol 7:280. https:\/\/doi.org\/10.3389\/fcimb.2017.00280","journal-title":"Front Cell Infect Microbiol"},{"issue":"4","key":"3122_CR18","doi-asserted-by":"publisher","first-page":"493","DOI":"10.4155\/fmc.15.6","volume":"7","author":"N Rabin","year":"2015","unstructured":"Rabin N, Zheng Y, Opoku-Temeng C, Du Y, Bonsu E, Sintim HO (2015) Biofilm formation mechanisms and targets for developing antibiofilm agents. Future Med Chem 7(4):493\u2013512. https:\/\/doi.org\/10.4155\/fmc.15.6","journal-title":"Future Med Chem"},{"issue":"1","key":"3122_CR19","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1186\/s12866-016-0736-1","volume":"16","author":"P Cools","year":"2016","unstructured":"Cools P, Ho E, Vranckx K, Schelstraete P, Wurth B, Franckx H et al (2016) Epidemic Achromobacter xylosoxidans strain among Belgian cystic fibrosis patients and review of literature. BMC Microbiol 16(1):122. https:\/\/doi.org\/10.1186\/s12866-016-0736-1","journal-title":"BMC Microbiol"},{"issue":"3","key":"3122_CR20","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1080\/08927014.2015.1124096","volume":"32","author":"AS Azevedo","year":"2016","unstructured":"Azevedo AS, Almeida C, Pereira B, Melo LF, Azevedo NF (2016) Impact of Delftia tsuruhatensis and Achromobacter xylosoxidans on Escherichia coli dual-species biofilms treated with antibiotic agents. Biofouling 32(3):227\u2013241. https:\/\/doi.org\/10.1080\/08927014.2015.1124096","journal-title":"Biofouling"},{"key":"3122_CR21","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1186\/1471-2180-10-140","volume":"10","author":"T Zhao","year":"2010","unstructured":"Zhao T, Liu Y (2010) N-acetylcysteine inhibit biofilms produced by Pseudomonas aeruginosa. BMC Microbiol 10:140. https:\/\/doi.org\/10.1186\/1471-2180-10-140","journal-title":"BMC Microbiol"},{"issue":"1","key":"3122_CR22","doi-asserted-by":"publisher","first-page":"212","DOI":"10.21037\/jtd.2017.11.130","volume":"10","author":"J Feng","year":"2018","unstructured":"Feng J, Liu B, Xu J, Wang Q, Huang L, Ou W et al (2018) In vitro effects of N-acetylcysteine alone and combined with tigecycline on planktonic cells and biofilms of Acinetobacter baumannii. J Thorac Dis 10(1):212\u2013218. https:\/\/doi.org\/10.21037\/jtd.2017.11.130","journal-title":"J Thorac Dis"},{"issue":"2","key":"3122_CR23","doi-asserted-by":"publisher","first-page":"106372","DOI":"10.1016\/j.ijantimicag.2021.106372","volume":"58","author":"A Aiyer","year":"2021","unstructured":"Aiyer A, Manoharan A, Paino D, Farrell J, Whiteley GS, Kriel FH et al (2021) Disruption of biofilms and killing of Burkholderia cenocepacia from cystic fibrosis lung using an antioxidant-antibiotic combination therapy. Int J Antimicrob Agents 58(2):106372. https:\/\/doi.org\/10.1016\/j.ijantimicag.2021.106372","journal-title":"Int J Antimicrob Agents"},{"issue":"10","key":"3122_CR24","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1016\/j.eimc.2012.11.011","volume":"31","author":"B Leite","year":"2013","unstructured":"Leite B, Gomes F, Teixeira P, Souza C, Pizzolitto E, Oliveira R (2013) Combined effect of linezolid and N-acetylcysteine against Staphylococcus epidermidis biofilms. Enferm Infecc Microbiol Clin 31(10):655\u2013659. https:\/\/doi.org\/10.1016\/j.eimc.2012.11.011","journal-title":"Enferm Infecc Microbiol Clin"},{"issue":"9","key":"3122_CR25","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1038\/ja.2016.9","volume":"69","author":"D Molina-Manso","year":"2016","unstructured":"Molina-Manso D, Del-Prado G, G\u00f3mez-Barrena E, Cordero-Ampuero J, Fernandez-Roblas R, Esteban J (2016) Effect of different agents with potential antibiofilm activity on antimicrobial susceptibility of biofilms formed by Staphylococcus spp. isolated from implant-related infections. J Antibiot 69(9):686\u2013688. https:\/\/doi.org\/10.1038\/ja.2016.9","journal-title":"J Antibiot"},{"issue":"12","key":"3122_CR26","doi-asserted-by":"publisher","first-page":"7513","DOI":"10.1128\/AAC.01334-16","volume":"60","author":"G Landini","year":"2016","unstructured":"Landini G, Di Maggio T, Sergio F, Docquier JD, Rossolini GM, Pallecchi L (2016) Effect of High N-Acetylcysteine concentrations on antibiotic activity against a large collection of respiratory pathogens. Antimicrob Agents Chemother 60(12):7513\u20137517. https:\/\/doi.org\/10.1128\/AAC.01334-16","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"3122_CR27","doi-asserted-by":"publisher","first-page":"3246","DOI":"10.1128\/AAC.00017-15","volume":"59","author":"J Rodr\u00edguez-Beltr\u00e1n","year":"2015","unstructured":"Rodr\u00edguez-Beltr\u00e1n J, Cabot G, Valencia EY, Costas C, Bou G, Oliver A et al (2015) N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism. Antimicrob Agents Chemother 59(6):3246\u20133251. https:\/\/doi.org\/10.1128\/AAC.00017-15","journal-title":"Antimicrob Agents Chemother"},{"key":"3122_CR28","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1128\/jcm.5.1.58-61.1977","volume":"5","author":"MF Parry","year":"1977","unstructured":"Parry MF, Neu HC (1977) Effect of N-acetylcysteine on antibiotic activity and bacterial growth in vitro. J Clin Microbiol 5:58\u201361. https:\/\/doi.org\/10.1128\/jcm.5.1.58-61.1977","journal-title":"J Clin Microbiol"},{"issue":"8","key":"3122_CR29","doi-asserted-by":"publisher","first-page":"3529","DOI":"10.1128\/AAC.00710-10","volume":"54","author":"M Goswami","year":"2010","unstructured":"Goswami M, Jawali N (2010) N-acetylcysteine-mediated modulation of bacterial antibiotic susceptibility. Antimicrob Agents Chemother 54(8):3529\u20133530. https:\/\/doi.org\/10.1128\/AAC.00710-10","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Current Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-022-03122-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00284-022-03122-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-022-03122-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T10:10:17Z","timestamp":1673345417000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00284-022-03122-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,25]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["3122"],"URL":"https:\/\/doi.org\/10.1007\/s00284-022-03122-x","relation":{},"ISSN":["0343-8651","1432-0991"],"issn-type":[{"value":"0343-8651","type":"print"},{"value":"1432-0991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,25]]},"assertion":[{"value":"7 April 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 November 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 November 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Not applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}}],"article-number":"5"}}