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Most contemporary antibiotics, however, are unable to eliminate biofilms. In recent years, with the application of new photosensitizers and the development of treatment, ALA-PDT (5-aminolevulinic acid photodynamic treatment) has achieved remarkable curative effect in the treatment of fungal infectious diseases; however, no research has been conducted on ALA-PDT against <jats:italic>A. fumigatus<\/jats:italic>. This study investigated the inhibitory effect of ALA-PDT at various 5-aminolevulinic acid concentrations and light doses on <jats:italic>A. fumigatus<\/jats:italic> planktonic and biofilms in vitro. We found that ALA-PDT may successfully inhibit the development of <jats:italic>A. fumigatus<\/jats:italic> biofilm and disintegrate mature biofilm. After ALA-PDT treatment, the adherence rate and vitality dramatically decreased, and the biofilm\u2019s structure was severely compromised. Our findings show for the first time that ALA-PDT may be used to prevent the formation of <jats:italic>A. fumigatus<\/jats:italic> biofilm and disturb the structure of mature biofilm, and that it could be employed as a therapeutic therapy for <jats:italic>A. fumigatus<\/jats:italic> superficial infection.<\/jats:p>","DOI":"10.1007\/s00284-023-03351-8","type":"journal-article","created":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T08:01:57Z","timestamp":1693641717000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of 5-Aminolevulinic Acid Photodynamic Therapy on Aspergillus fumigatus Biofilms in Vitro"],"prefix":"10.1007","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2698-5288","authenticated-orcid":false,"given":"Zhimin","family":"Duan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2722-8673","authenticated-orcid":false,"given":"Jianbo","family":"Tong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8905-2346","authenticated-orcid":false,"given":"Nana","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3196-5406","authenticated-orcid":false,"given":"Rong","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1660-1578","authenticated-orcid":false,"given":"Yuzhen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1330-2326","authenticated-orcid":false,"given":"Min","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,9,2]]},"reference":[{"key":"3351_CR1","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1186\/s12879-017-2646-8","volume":"17","author":"F Gallais","year":"2017","unstructured":"Gallais F, Denis J, Koobar O et al (2017) Simultaneous primary invasive cutaneous aspergillosis in two preterm twins: case report and review of the literature. 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All authors have given consent for publication.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}}],"article-number":"334"}}