{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T05:02:54Z","timestamp":1748667774832,"version":"3.37.3"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100006507","name":"Funda\u00e7\u00e3o Oswaldo Cruz","doi-asserted-by":"publisher","award":["31081634214-4298"],"award-info":[{"award-number":["31081634214-4298"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.643393\/2021-00","88887.369261\/2019-00","88887.149114\/2017-00"],"award-info":[{"award-number":["88887.643393\/2021-00","88887.369261\/2019-00","88887.149114\/2017-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["140434\/2022-4","311769\/2023-3"],"award-info":[{"award-number":["140434\/2022-4","311769\/2023-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Curr Microbiol"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1007\/s00284-024-03847-x","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T09:02:45Z","timestamp":1725008565000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of Brucella S19 Vaccine Strain DNA in Domestic and Wild Ungulates from Brazilian Pantanal"],"prefix":"10.1007","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4596-5733","authenticated-orcid":false,"given":"Gabriel","family":"Carvalho de Macedo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6746-7647","authenticated-orcid":false,"given":"Carolina Santos Pereira Cardoso","family":"Trindade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4691-2617","authenticated-orcid":false,"given":"Caroline Paes","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9632-5173","authenticated-orcid":false,"given":"Luiz Gustavo Rodrigues Oliveira","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2032-8129","authenticated-orcid":false,"given":"Filipe Martins","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3327-0800","authenticated-orcid":false,"given":"William Oliveira","family":"de Assis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9703-0577","authenticated-orcid":false,"given":"Alinne Pereira","family":"de Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1459-7931","authenticated-orcid":false,"given":"Emily Rebeca Antunes","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7267-9310","authenticated-orcid":false,"given":"S\u00e1vio Freire","family":"Bruno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2404-8765","authenticated-orcid":false,"given":"Heitor Miraglia","family":"Herrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9240-4376","authenticated-orcid":false,"given":"Carina","family":"Elisei de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,8,30]]},"reference":[{"key":"3847_CR1","unstructured":"Corbel MJ (2006) Brucellosis in Humans and Animals. World Health Organization, Food and Agriculture Organization, World Organization for Animal Health. http:\/\/www.who.int\/csr\/resources\/publications\/Brucellosis.pdf. Accessed 12 June 2022"},{"key":"3847_CR2","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1590\/S0100-736X2013000600012","volume":"33","author":"RL Santos","year":"2013","unstructured":"Santos RL, Martins TM, Borges AM, Paix\u00e3o TA (2013) Economic losses due to bovine brucellosis in Brazil. Pesq Vet Bras 33:759\u2013764. https:\/\/doi.org\/10.1590\/S0100-736X2013000600012","journal-title":"Pesq Vet Bras"},{"key":"3847_CR3","unstructured":"Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento (MAPA) (2006) Programa Nacional de Controle e Erradica\u00e7\u00e3o da Brucelose e da Tuberculose Animal (PNCEBT): Manual t\u00e9cnico. :\/\/www.adepara.pa.gov.br\/sites\/default\/files\/Ma-nual%20do%20PNCEBT%20-%20Original.pdf. Accessed 26 Aug 2022"},{"key":"3847_CR4","unstructured":"Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento (MAPA) (2007) Secretaria de Defesa Agropecu\u00e1ria. Instru\u00e7\u00e3o Normativa n\u00ba 33, de 24 de agosto de 2007. Di\u00e1rio Oficial da Uni\u00e3o, Bras\u00edlia, 24 ago. 2007. Se\u00e7\u00e3o 1, p. 6"},{"key":"3847_CR5","first-page":"1","volume":"99","author":"LOF Oliveira","year":"2016","unstructured":"Oliveira LOF, Abreu UGP, Dias FRT, Fernandes FA, Nogueira E, Silva JD (2016) Bovine population estimates for the Pantanal based on thematic models and traditional indexes. Comunicado T\u00e9cnico Embrapa Pantanal 99:1\u201311","journal-title":"Comunicado T\u00e9cnico Embrapa Pantanal"},{"issue":"Suppl 1","key":"3847_CR6","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1590\/S1519-69842011000200009","volume":"71","author":"CJ Alho","year":"2011","unstructured":"Alho CJ, Camargo G, Fischer E (2011) Terrestrial and aquatic mammals of the Pantanal. Braz J Biol 71(Suppl 1):297\u2013310. https:\/\/doi.org\/10.1590\/S1519-69842011000200009","journal-title":"Braz J Biol"},{"key":"3847_CR7","first-page":"1","volume":"58","author":"AO Pellegrin","year":"2006","unstructured":"Pellegrin AO, Leite RMH, Sereno JRB, Lage AP, Leite RC, Ravaglia E (2006) Brucelose Bovina no Pantanal Sul-Mato-Grossense: dados preliminares. Comunicado T\u00e9cnico Embrapa Pantanal 58:1\u20134","journal-title":"Comunicado T\u00e9cnico Embrapa Pantanal"},{"issue":"S1","key":"3847_CR8","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1590\/S0102-09352009000700007","volume":"61","author":"SC Chate","year":"2009","unstructured":"Chate SC, Dias RA, Amaku M, Ferreira F, Moraes GM, Costa Neto AA, Monteiro LARC, L\u00f4bo JR, Figueiredo VCF, Gon\u00e7alves VSP, Ferreira Neto JS (2009) Epidemiological situations of bovine brucellosis in the State of Mato Grosso do Sul. Brazil Arq Bras Med Vet Zootec 61(S1):46\u201355. https:\/\/doi.org\/10.1590\/S0102-09352009000700007","journal-title":"Brazil Arq Bras Med Vet Zootec"},{"key":"3847_CR9","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1590\/S0102-09352009000700008","volume":"61","author":"RL Negreiros","year":"2009","unstructured":"Negreiros RL, Dias RA, Ferreira F, Ferreira Neto JS, Gon\u00e7alves VSP, Silva MCP, Figueiredo VCF, L\u00f4bo JR, Freitas J, Amaku M (2009) Epidemiologic situation of bovine brucellosis in the State of Mato Grosso, Brazil. Arq Bras Med Vet Zootec 61:56\u201365. https:\/\/doi.org\/10.1590\/S0102-09352009000700008","journal-title":"Arq Bras Med Vet Zootec"},{"issue":"11","key":"3847_CR10","doi-asserted-by":"publisher","first-page":"e0143816","DOI":"10.1371\/journal.pone.0143816","volume":"10","author":"MM Furtado","year":"2015","unstructured":"Furtado MM, Gennari SM, Ikuta CY, J\u00e1como ATA, Morais ZM, Pena HFJ, Porf\u00edrio GEO, Sollamann R, Souza GO, T\u00f4rres NM, Neto JSF (2015) Serosurvey of smooth Brucella, Leptospira spp. and Toxoplasma gondii in free-ranging jaguars (Panthera onca) and domestic animals from Brazil. PLoS One 10(11):e0143816. https:\/\/doi.org\/10.1371\/journal.pone.0143816","journal-title":"PLoS One"},{"issue":"5","key":"3847_CR11","doi-asserted-by":"publisher","first-page":"3467","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3467","volume":"37","author":"JM Leal Filho","year":"2016","unstructured":"Leal Filho JM, Bottene IFN, Monteiro LARC, Pellegrin AO, Gon\u00e7alves VSP, Ferreira F, Dias RA, Amaku M, Telles EO, Grisi-Filho JHH, Heinemann MB, Ferreira Neto JS (2016) Control of bovine brucellosis from 1998 to 2009 in the state of Mato Grosso do Sul. Brazil Semina: Ci\u00eancias Agr\u00e1rias 37(5):3467\u20133478. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3467","journal-title":"Brazil Semina: Ci\u00eancias Agr\u00e1rias"},{"issue":"6","key":"3847_CR12","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1590\/S0100-736X2010000600006","volume":"30","author":"C Elisei","year":"2010","unstructured":"Elisei C, Pellegrin A, Tomas WM, Soares CO, Ara\u00fajo FR, Funes-Huacca ME, Rosinha GMS (2010) Molecular evidence of Brucella sp. in deer (Ozotoceros bezoarticus) of the southern Pantanal. Pesq Vet Bras 30(6):503\u2013509. https:\/\/doi.org\/10.1590\/S0100-736X2010000600006","journal-title":"Pesq Vet Bras"},{"issue":"12","key":"3847_CR13","doi-asserted-by":"publisher","first-page":"2193","DOI":"10.1590\/0103-8478cr20131167","volume":"44","author":"EMS Dorneles","year":"2014","unstructured":"Dorneles EMS, Pellegrin AO, Schabib-P\u00e9res IAHF, Mathias LA, Mour\u00e3o G, Bianchi RC, Olifers N, Rocha FL, Lage AP (2014) Serology for brucellosis in free-ranging crab-eating foxes (Cerdocyon thous) and brown-nosed coatis (Nasua nasua) from Brazilian Pantanal. Ci\u00eancia Rural 44(12):2193\u20132196. https:\/\/doi.org\/10.1590\/0103-8478cr20131167","journal-title":"Ci\u00eancia Rural"},{"issue":"1","key":"3847_CR14","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1007\/s42770-018-0006-5","volume":"50","author":"ALB de Oliveira","year":"2019","unstructured":"de Oliveira ALB, de Macedo GC, Rosinha GMS, Melgarejo JL, Alves AGL, Barreto WTG, Santos FM, Campos JBV, Herrera HM, de Oliveira CE (2019) Detection of Brucella spp. in dogs at Pantanal wetlands. Braz J Microbiol 50(1):307\u2013312. https:\/\/doi.org\/10.1007\/s42770-018-0006-5","journal-title":"Braz J Microbiol"},{"issue":"4","key":"3847_CR15","doi-asserted-by":"publisher","first-page":"2287","DOI":"10.1007\/s42770-022-00831-0","volume":"53","author":"GC de Macedo","year":"2020","unstructured":"de Macedo GC, Herrera HM, de Oliveira Porf\u00edrio GE, Santos FM, de Assis WO, de Andrade GB, Nantes WAG, de Mendoza JH, Fern\u00e1ndez-Llario P, de Oliveira CE (2020) Brucellosis in the Brazilian Pantanal wetland: threat to animal production and wildlife conservation. Braz J Microbiol 53(4):2287\u20132297. https:\/\/doi.org\/10.1007\/s42770-022-00831-0","journal-title":"Braz J Microbiol"},{"issue":"1","key":"3847_CR16","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1590\/S1517-83822007000100005","volume":"38","author":"S Miyashiro","year":"2007","unstructured":"Miyashiro S, Scarcelli E, Piatti RM, Campos FR, Vialta A, Keid LB, Dias RA, Genovez ME (2007) Detection of Brucella abortus DNA in illegal cheese from S\u00e3o Paulo and Minas Gerais and differentiation of B19 vaccinal strain by means of the Polymerase chain reaction (PCR). Braz J Microbiol 38(1):17\u201322. https:\/\/doi.org\/10.1590\/S1517-83822007000100005","journal-title":"Braz J Microbiol"},{"issue":"2","key":"3847_CR17","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1590\/S1517-83822012000200022","volume":"43","author":"WA Pacheco","year":"2012","unstructured":"Pacheco WA, Genovez ME, Pozzi CR, Silva LMP, Azevedo SS, Did CC, Piatti RM, Pinheiro ES, Castro V, Miyashiro S, Gambarini ML (2012) Excretion of Brucella abortus vaccine B19 strain during a reproductive cycle in dairy cows. Braz J Microbiol 43(2):594\u2013601. https:\/\/doi.org\/10.1590\/S1517-83822012000200022","journal-title":"Braz J Microbiol"},{"issue":"2","key":"3847_CR18","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1111\/tbed.12727","volume":"65","author":"DG Junqueira Junior","year":"2017","unstructured":"Junqueira Junior DG, Lima AMC, Rosinha GMS, Carvalho CEG, Oliveira CE, Sanches CC (2017) Detection of Brucella abortus B19 strain DNA in seminal plasma by polymerase chain reaction in Brazil. Transbound Emerg Dis 65(2):476\u2013479. https:\/\/doi.org\/10.1111\/tbed.12727","journal-title":"Transbound Emerg Dis"},{"key":"3847_CR19","first-page":"631","volume-title":"Molecular cloning: a laboratory manual","author":"J Sambrook","year":"2001","unstructured":"Sambrook J, Russel DW (2001) Rapid isolation of yeast DNA. In: Sambrook J, Russel DW (eds) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York, pp 631\u2013632"},{"issue":"4","key":"3847_CR20","first-page":"271","volume":"95","author":"GG Baily","year":"1992","unstructured":"Baily GG, Krahn JB, Drasar BS, Stoker NG (1992) Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 95(4):271\u2013275","journal-title":"J Trop Med Hyg"},{"issue":"3","key":"3847_CR21","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1111\/j.1574-6968.1994.tb07123.x","volume":"121","author":"FJ Sangari","year":"1994","unstructured":"Sangari FJ, Garc\u00eda-Lobo JM, Ag\u00fcero J (1994) The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbio Lett 121(3):337\u2013342. https:\/\/doi.org\/10.1111\/j.1574-6968.1994.tb07123.x","journal-title":"FEMS Microbio Lett"},{"issue":"7","key":"3847_CR22","doi-asserted-by":"publisher","first-page":"4043","DOI":"10.1099\/ijsem.0.004244","volume":"70","author":"A Oren","year":"2020","unstructured":"Oren A, Garrity G (2020) List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol 70(7):4043\u20134049. https:\/\/doi.org\/10.1099\/ijsem.0.004244","journal-title":"Int J Syst Evol Microbiol"},{"issue":"8","key":"3847_CR23","doi-asserted-by":"publisher","first-page":"615","DOI":"10.12935\/jvma.63.615","volume":"63","author":"K Yamamoto","year":"2010","unstructured":"Yamamoto K, Tamura T, Okamura Y, Kabamoto A, Moriwaki S, Eto M (2010) Isolation and characterization of Ochrobactrum intermedium from imported cattle serologically diagnosed with bovine brucellosis. J Jpn Vet Med Assoc 63(8):615\u2013619. https:\/\/doi.org\/10.12935\/jvma.63.615","journal-title":"J Jpn Vet Med Assoc"},{"issue":"2","key":"3847_CR24","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1007\/pl00011242","volume":"20","author":"MC Casa\u00f1as","year":"2001","unstructured":"Casa\u00f1as MC, Queipo-Ortu\u00f1o MI, Rodriguez-Torres A, Ordu\u00f1a A, Colmenero JD, Morata P (2001) Specificity of a polymerase chain reaction assay of a target sequence on the 31-kilodalton Brucella antigen DNA used to diagnose human brucellosis. Eur J Clin Microbiol Infect Dis 20(2):127\u2013131. https:\/\/doi.org\/10.1007\/pl00011242","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"3847_CR25","first-page":"305","volume-title":"Laz razas porcinas Iberoamericanas: um enfoque etnozoot\u00e9cnico","author":"U Piovezan","year":"2014","unstructured":"Piovezan U (2014) O porco monteiro do Pantanal, Brasil. In: Silva Filha OL (ed) Laz razas porcinas Iberoamericanas: um enfoque etnozoot\u00e9cnico. Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia Baiano, Salvador, pp 305\u2013306"},{"key":"3847_CR26","unstructured":"Cust\u00f3dio MS (2013) Molecular identification of Brucella spp. in feral swines (Sus scrofa) from Mato Grosso do Sul Pantanal \u2013 Brazil. Thesis. Federal University of Mato Grosso do Sul"},{"issue":"3","key":"3847_CR27","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1177\/104063870701900301","volume":"19","author":"WC Stoffregen","year":"2007","unstructured":"Stoffregen WC, Olsen SC, Jack Wheeler C, Bricker BJ, Palmer MV, Jensen AE, Halling SM, Alt DP (2007) Diagnostic characterization of a feral swine herd enzootically infected with Brucella. J Vet Diagn Invest 19(3):227\u2013237. https:\/\/doi.org\/10.1177\/104063870701900301","journal-title":"J Vet Diagn Invest"},{"issue":"4","key":"3847_CR28","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1016\/0264-410x(95)00214-l","volume":"14","author":"FJ Sangari","year":"1996","unstructured":"Sangari FJ, Ag\u00fcero J, Garc\u00eda-Lobo JM (1996) Improvement of the Brucella abortus B19 vaccine by its preparation in a glycerol based medium. Vaccine 14(4):274\u2013276. https:\/\/doi.org\/10.1016\/0264-410x(95)00214-l","journal-title":"Vaccine"},{"key":"3847_CR29","first-page":"1","volume":"27","author":"JRB Sereno","year":"2002","unstructured":"Sereno JRB, Pellegrin AO, Ormay JN (2002) Manejo reprodutivo e sanit\u00e1rio para bovinos de corte do Pantanal \u2013 1 \u2013 Calend\u00e1rio profil\u00e1tico. Comunicado T\u00e9cnico Embrapa Pantanal 27:1\u20134","journal-title":"Comunicado T\u00e9cnico Embrapa Pantanal"},{"key":"3847_CR30","unstructured":"Desbiez, ALJ (2007) Wildlife conservation in the Pantanal: habitat alteration, invasive species and bushmeat hunting. Dissertation, University of Kent"}],"container-title":["Current Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-024-03847-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00284-024-03847-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-024-03847-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T16:05:22Z","timestamp":1726675522000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00284-024-03847-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,30]]},"references-count":30,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["3847"],"URL":"https:\/\/doi.org\/10.1007\/s00284-024-03847-x","relation":{},"ISSN":["0343-8651","1432-0991"],"issn-type":[{"type":"print","value":"0343-8651"},{"type":"electronic","value":"1432-0991"}],"subject":[],"published":{"date-parts":[[2024,8,30]]},"assertion":[{"value":"16 April 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 August 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 August 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This work was conducted in accordance to the Instituto Chico Mendes de Biodiversidade (ICMBIO) (n\u00b0 64616-1) and the Ethics Committee for Animal Use (CEUA) of the Dom Bosco Catholic University (n\u00ba 22\/2018).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"All authors agree to publish.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}}],"article-number":"333"}}