{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T11:53:13Z","timestamp":1768564393395,"version":"3.49.0"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T00:00:00Z","timestamp":1747612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T00:00:00Z","timestamp":1747612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Curr Microbiol"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1007\/s00284-025-04275-1","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T16:02:40Z","timestamp":1747670560000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Loop 3 Repeats in Omp34 is a Promising Immunogen for Vaccine Development Against Acinetobacter baumannii Infections"],"prefix":"10.1007","volume":"82","author":[{"given":"Roghayeh","family":"Bashiri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7263-901X","authenticated-orcid":false,"given":"Abolfazl","family":"Jahangiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1689-8547","authenticated-orcid":false,"given":"Saeede","family":"Masoomkhani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0530-4324","authenticated-orcid":false,"given":"Iraj","family":"Rasooli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,5,19]]},"reference":[{"key":"4275_CR1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.3389\/fcimb.2017.00055","volume":"7","author":"CR Lee","year":"2017","unstructured":"Lee CR, Lee JH, Park M, Park KS, Bae IK, Kim YB, Cha CJ, Jeong BC, Lee SH (2017) Biology of Acinetobacter baumannii: pathogenesis, antibiotic resistance mechanisms, and prospective treatment options. Front Cell Infect Microbiol 7:55. https:\/\/doi.org\/10.3389\/fcimb.2017.00055","journal-title":"Front Cell Infect Microbiol"},{"key":"4275_CR2","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.micpath.2018.12.039","volume":"128","author":"NM Elhosseiny","year":"2019","unstructured":"Elhosseiny NM, Elhezawy NB, Attia AS (2019) Comparative proteomics analyses of Acinetobacter baumannii strains ATCC 17978 and AB5075 reveal the differential role of type II secretion system secretomes in lung colonization and ciprofloxacin resistance. Microbial Pathog 128:20\u201327. https:\/\/doi.org\/10.1016\/j.micpath.2018.12.039","journal-title":"Microbial Pathog"},{"issue":"1","key":"4275_CR3","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.yexcr.2018.01.035","volume":"364","author":"M Dai","year":"2018","unstructured":"Dai M, Pan P, Li H, Liu S, Zhang L, Song C, Li Y, Li Q, Mao Z, Long Y, Su X, Hu C (2018) The antimicrobial cathelicidin peptide hlF(1\u201311) attenuates alveolar macrophage pyroptosis induced by Acinetobacter baumannii in vivo. Exp Cell Res 364(1):95\u2013103. https:\/\/doi.org\/10.1016\/j.yexcr.2018.01.035","journal-title":"Exp Cell Res"},{"issue":"3","key":"4275_CR4","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1111\/2049-632x.12125","volume":"71","author":"LC Antunes","year":"2014","unstructured":"Antunes LC, Visca P, Towner KJ (2014) Acinetobacter baumannii: evolution of a global pathogen. Pathog Dis 71(3):292\u2013301. https:\/\/doi.org\/10.1111\/2049-632x.12125","journal-title":"Pathog Dis"},{"issue":"6","key":"4275_CR5","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1016\/j.jinf.2020.10.016","volume":"81","author":"P Gallagher","year":"2020","unstructured":"Gallagher P, Baker S (2020) Developing new therapeutic approaches for treating infections caused by multi-drug resistant Acinetobacter baumannii: Acinetobacter baumannii therapeutics. J Infection 81(6):857\u2013861. https:\/\/doi.org\/10.1016\/j.jinf.2020.10.016","journal-title":"J Infection"},{"issue":"6","key":"4275_CR6","doi-asserted-by":"publisher","first-page":"935","DOI":"10.3390\/microorganisms8060935","volume":"8","author":"CO Vrancianu","year":"2020","unstructured":"Vrancianu CO, Gheorghe I, Czobor IB, Chifiriuc MC (2020) Antibiotic resistance profiles, molecular mechanisms and innovative treatment strategies of Acinetobacter baumannii. Microorganisms 8(6):935. https:\/\/doi.org\/10.3390\/microorganisms8060935","journal-title":"Microorganisms"},{"key":"4275_CR7","doi-asserted-by":"publisher","first-page":"120","DOI":"10.3389\/fmicb.2023.1049917","volume":"14","author":"Y Hu","year":"2023","unstructured":"Hu Y, Lyu Y, Jia X, Yue C, Deng S, Zhang X (2023) Non-antibiotic prevention and treatment against Acinetobacter baumannii infection: are vaccines and adjuvants effective strategies? Front Microbiol 14:120. https:\/\/doi.org\/10.3389\/fmicb.2023.1049917","journal-title":"Front Microbiol"},{"key":"4275_CR8","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10030373","author":"I Kyriakidis","year":"2021","unstructured":"Kyriakidis I, Vasileiou E, Pana ZD, Tragiannidis A (2021) Acinetobacter baumannii antibiotic resistance mechanisms. Pathogens. https:\/\/doi.org\/10.3390\/pathogens10030373","journal-title":"Pathogens"},{"key":"4275_CR9","doi-asserted-by":"publisher","first-page":"1601","DOI":"10.3389\/fmicb.2019.01601","volume":"10","author":"FC Morris","year":"2019","unstructured":"Morris FC, Dexter C, Kostoulias X, Uddin MI, Peleg AY (2019) The mechanisms of disease caused by Acinetobacter baumannii. Front Microbiol 10:1601. https:\/\/doi.org\/10.3389\/fmicb.2019.01601","journal-title":"Front Microbiol"},{"key":"4275_CR10","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.mimet.2017.11.008","volume":"144","author":"A Jahangiri","year":"2018","unstructured":"Jahangiri A, Rasooli I, Owlia P, Imani Fooladi AA, Salimian J (2018) Highly conserved exposed immunogenic peptides of Omp34 against Acinetobacter baumannii: an innovative approach. J Microbiol Methods 144:79\u201385. https:\/\/doi.org\/10.1016\/j.mimet.2017.11.008","journal-title":"J Microbiol Methods"},{"key":"4275_CR11","doi-asserted-by":"publisher","first-page":"108731","DOI":"10.1016\/j.intimp.2022.108731","volume":"108","author":"Z Akbari","year":"2022","unstructured":"Akbari Z, Rasooli I, Ghaini MH, Chaudhuri S, Andisi VF, Jahangiri A, Ramezanalizadeh F, Schryvers AB (2022) BauA and Omp34 surface loops trigger protective antibodies against Acinetobacter baumannii in a murine sepsis model. Int Immunopharmacol 108:108731. https:\/\/doi.org\/10.1016\/j.intimp.2022.108731","journal-title":"Int Immunopharmacol"},{"issue":"4","key":"4275_CR12","doi-asserted-by":"publisher","first-page":"387","DOI":"10.3390\/pathogens10040387","volume":"10","author":"M Sarshar","year":"2021","unstructured":"Sarshar M, Behzadi P, Scribano D, Palamara AT, Ambrosi C (2021) Acinetobacter baumannii: an ancient commensal with weapons of a pathogen. Pathogens 10(4):387. https:\/\/doi.org\/10.3390\/pathogens10040387","journal-title":"Pathogens"},{"issue":"Pt B","key":"4275_CR13","doi-asserted-by":"publisher","first-page":"105291","DOI":"10.1016\/j.micpath.2021.105291","volume":"161","author":"A Naghipour Erami","year":"2021","unstructured":"Naghipour Erami A, Rasooli I, Jahangiri A, Darvish Alipour Astaneh S (2021) Anti-Omp34 antibodies protect against Acinetobacter baumannii in a murine sepsis model. Microb Pathog 161(Pt B):105291. https:\/\/doi.org\/10.1016\/j.micpath.2021.105291","journal-title":"Microb Pathog"},{"key":"4275_CR14","doi-asserted-by":"publisher","first-page":"109013","DOI":"10.1016\/j.intimp.2022.109013","volume":"110","author":"F Golestani","year":"2022","unstructured":"Golestani F, Malekan M, Rasooli I, Jahangiri A, Ramezanalizadeh F, Chaudhuri S, Andisi VF, Schryvers AB (2022) Immunogenicity of loop 3 of Omp34 from A. Baumannii in loopless C-lobe of TbpB of N. meningitidis. Int Immunopharmacol 110:109013. https:\/\/doi.org\/10.1016\/j.intimp.2022.109013","journal-title":"Int Immunopharmacol"},{"key":"4275_CR15","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.molimm.2022.06.010","volume":"149","author":"M Mesbahi Moghaddam","year":"2022","unstructured":"Mesbahi Moghaddam M, Rasooli I, Ghaini MH, Jahangiri A, Ramezanalizadeh F, Ghasemkhah Tootkleh R (2022) Immunoprotective characterization of egg yolk immunoglobulin raised to loop 3 of outer membrane protein 34 (Omp34) in a murine model against Acinetobacter baumannii. Mol Immunol 149:87\u201393. https:\/\/doi.org\/10.1016\/j.molimm.2022.06.010","journal-title":"Mol Immunol"},{"key":"4275_CR16","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.compbiolchem.2018.01.003","volume":"72","author":"A Jahangiri","year":"2018","unstructured":"Jahangiri A, Rasooli I, Owlia P, Fooladi AAI, Salimian J (2018) An integrative in silico approach to the structure of Omp33\u201336 in Acinetobacter baumannii. Comput Biol Chem 72:77\u201386. https:\/\/doi.org\/10.1016\/j.compbiolchem.2018.01.003","journal-title":"Comput Biol Chem"},{"issue":"2","key":"4275_CR17","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1111\/jam.14135","volume":"126","author":"A Jahangiri","year":"2019","unstructured":"Jahangiri A, Owlia P, Rasooli I, Salimian J, Derakhshanifar E, Naghipour Erami A, Darzi Eslam E, Darvish Alipour Astaneh S (2019) Specific egg yolk antibodies (IgY) confer protection against Acinetobacter baumannii in a murine pneumonia model. J Appl Microbiol 126(2):624\u2013632. https:\/\/doi.org\/10.1111\/jam.14135","journal-title":"J Appl Microbiol"},{"issue":"1","key":"4275_CR18","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.pep.2005.01.016","volume":"41","author":"FW Studier","year":"2005","unstructured":"Studier FW (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41(1):207\u2013234. https:\/\/doi.org\/10.1016\/j.pep.2005.01.016","journal-title":"Protein Expr Purif"},{"key":"4275_CR19","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/978-1-59745-198-7_4","volume-title":"The protein protocols handbook","author":"NJ Kruger","year":"2009","unstructured":"Kruger NJ (2009) The bradford method for protein quantitation. In: Walker JM (ed) The protein protocols handbook. Humana Press, Totowa, pp 17\u201324"},{"issue":"4","key":"4275_CR20","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1089\/mab.2013.0073","volume":"33","author":"A Baig","year":"2014","unstructured":"Baig A, Cabral TM, Corbett CR (2014) Development and characterization of monoclonal antibodies for rapid detection of Acinetobacter baumannii. Monoclon Antib Immunodiagn Immunother 33(4):291\u2013298. https:\/\/doi.org\/10.1089\/mab.2013.0073","journal-title":"Monoclon Antib Immunodiagn Immunother"},{"issue":"Pt A","key":"4275_CR21","doi-asserted-by":"publisher","first-page":"105874","DOI":"10.1016\/j.micpath.2022.105874","volume":"173","author":"M Tamehri","year":"2022","unstructured":"Tamehri M, Rasooli I, Pishgahi M, Jahangiri A, Ramezanalizadeh F, Banisaeed Langroodi SR (2022) Combination of BauA and OmpA elicit immunoprotection against Acinetobacter baumannii in a murine sepsis model. Microb Pathog 173(Pt A):105874. https:\/\/doi.org\/10.1016\/j.micpath.2022.105874","journal-title":"Microb Pathog"},{"issue":"2872","key":"4275_CR22","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1126\/science.111.2872.42.b","volume":"111","author":"J Cornfield","year":"1950","unstructured":"Cornfield J (1950) The probit method. Science 111(2872):42\u201343. https:\/\/doi.org\/10.1126\/science.111.2872.42.b","journal-title":"Science"},{"issue":"1","key":"4275_CR23","doi-asserted-by":"publisher","first-page":"111","DOI":"10.3109\/01480548309072465","volume":"6","author":"HR Lieberman","year":"1983","unstructured":"Lieberman HR (1983) Estimating LD50 using the probit technique: a BASIC computer program. Drug Chem Toxicol 6(1):111\u2013116. https:\/\/doi.org\/10.3109\/01480548309072465","journal-title":"Drug Chem Toxicol"},{"issue":"12","key":"4275_CR24","doi-asserted-by":"publisher","first-page":"e0189621","DOI":"10.1371\/journal.pone.0189621","volume":"12","author":"RC Founou","year":"2017","unstructured":"Founou RC, Founou LL, Essack SY (2017) Clinical and economic impact of antibiotic resistance in developing countries: a systematic review and meta-analysis. PLoS ONE 12(12):e0189621. https:\/\/doi.org\/10.1371\/journal.pone.0189621","journal-title":"PLoS ONE"},{"issue":"8","key":"4275_CR25","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1086\/533452","volume":"197","author":"LB Rice","year":"2008","unstructured":"Rice LB (2008) Federal funding for the study of antimicrobial resistance in nosocomial pathogens: no ESKAPE. J Infect Dis 197(8):1079\u20131081. https:\/\/doi.org\/10.1086\/533452","journal-title":"J Infect Dis"},{"issue":"3","key":"4275_CR26","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/s1473-3099(17)30753-3","volume":"18","author":"E Tacconelli","year":"2018","unstructured":"Tacconelli E, Carrara E, Savoldi A, Harbarth S, Mendelson M, Monnet DL, Pulcini C, Kahlmeter G, Kluytmans J, Carmeli Y, Ouellette M, Outterson K, Patel J, Cavaleri M, Cox EM, Houchens CR, Grayson ML, Hansen P, Singh N, Theuretzbacher U, Magrini N (2018) Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Dis 18(3):318\u2013327. https:\/\/doi.org\/10.1016\/s1473-3099(17)30753-3","journal-title":"Lancet Infect Dis"},{"issue":"6","key":"4275_CR27","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1016\/j.eururo.2022.08.023","volume":"82","author":"FME Wagenlehner","year":"2022","unstructured":"Wagenlehner FME, Dittmar F (2022) Re: global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Eur Urol 82(6):658. https:\/\/doi.org\/10.1016\/j.eururo.2022.08.023","journal-title":"Eur Urol"}],"container-title":["Current Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-025-04275-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00284-025-04275-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-025-04275-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T02:17:49Z","timestamp":1749867469000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00284-025-04275-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,19]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["4275"],"URL":"https:\/\/doi.org\/10.1007\/s00284-025-04275-1","relation":{},"ISSN":["0343-8651","1432-0991"],"issn-type":[{"value":"0343-8651","type":"print"},{"value":"1432-0991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,19]]},"assertion":[{"value":"7 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 May 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 May 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The ethics committee of Shahed University approved the animal care protocol with the code IR.SHAHED.REC.1401.111.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}],"article-number":"292"}}