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Subjects or patients with symptoms of tuberculosis disease like cough for 2 weeks or more, weight loss, fever, and night sweats reporting to the outpatient department of the hospital were recruited after receiving their signed informed consent forms by the individual or their concerned guardian. Sputum samples (day 0 and day 1) of these subjects were taken for sputum microscopy at the hospital and culture test for drug sensitivity test using Mycobacteria Growth Indicator Tube (MGIT) in BACTEC MGIT 960 System at a National Accreditation Board for Testing and Calibration Laboratories (NABL) accredited laboratory following WHO guidelines. All the methods and experimental procedure related with clinical samples was carried out in accordance with ethical guidelines and regulations.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval and Consent to Participate"}}],"article-number":"102"}}