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Enzymatic bioremediation emerges as a sustainable alternative for the mitigation of these contaminants. This study investigated the potential of the metagenomic laccase, LacMeta, expressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    BL21 (DE3), to degrade imazapic using a whole-cell approach. LacMeta expression was optimized with CuSO\u2084, which proved to be four times more effective than IPTG. The\n                    <jats:italic>E. coli<\/jats:italic>\n                    \u2009+\u2009LacMeta cells demonstrated high tolerance to the herbicide, maintaining cell viability even at high doses (350\u00a0g\/ha). Notably, the enzymatic activity of LacMeta was not inhibited by imazapic; on the contrary, it was stimulated, reaching a specific activity nearly three times higher in the presence of the herbicide compared to the control. Degradation was confirmed by UV-Visible spectroscopy, which showed the disappearance of imazapic\u2019s characteristic peaks (200\u2013280\u00a0nm) over 15 days. \u00b9H-NMR and FTIR analyses corroborated the degradation, indicating structural changes in the herbicide molecule, particularly in the aromatic ring region (signals at 8.25 and 8.50 ppm). Phytotoxicity assays with lettuce seeds (\n                    <jats:italic>Lactuca sativa<\/jats:italic>\n                    ) confirmed that treatment with the LacMeta-containing supernatant cell free significantly reduced the toxicity of imazapic in the soil. The results demonstrate that LacMeta has high potential for the bioremediation of imazapic, and the whole-cell approach represents a promising and cost-effective strategy for the decontamination of environments impacted by this herbicide.\n                  <\/jats:p>","DOI":"10.1007\/s00284-026-04760-1","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T06:43:36Z","timestamp":1771829016000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficiency of Imazapic Degradation: an Assessment of LacMeta Treatments Utilizing Whole Cell"],"prefix":"10.1007","volume":"83","author":[{"given":"Nat\u00e1lia Sarmanho Monteiro","family":"Lima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elis\u00e2ngela Soares","family":"Gomes-Pepe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio Vinicius Crizostomo","family":"Kock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Alberto","family":"Colnago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Luis","family":"da Costa Aguiar Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana Gertrudes","family":"de Macedo Lemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"4760_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matpr.2020.05.722","author":"M Narayanan","year":"2020","unstructured":"Narayanan M, Kumarasamy S, Ranganathan M et al (2020) Enzyme and metabolites attained in degradation of chemical pesticides \u00df Cypermethrin by Bacillus cereus. 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