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Among\n                    <jats:italic>Enterobacterales<\/jats:italic>\n                    , IncHI2\/IncHI2A plasmids are of particular concern, as they combine broad host range, conjugative potential, and mosaic architecture enriched with antimicrobial resistance genes (ARGs), biocide tolerance, and heavy-metal resistance. This study provides the first systematic comparative genomics of\n                    <jats:italic>Pseudescherichia vulneris<\/jats:italic>\n                    , an underrecognized yet genomically versatile species at the human\u2013animal\u2013environment interface. All 30 publicly available genomes were analyzed to reconstruct the pangenome, resistome, virulome, and associated MGEs. The pangenome was open, reflecting ongoing diversification and strong potential for horizontal gene acquisition. Resistomes were highly heterogeneous, ranging from minimal repertoires in most animal and environmental isolates to multidrug-resistance profiles in hospital-associated and occasional animal genomes. Clinically significant determinants, including\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    <jats:italic>, bla<\/jats:italic>\n                    <jats:sub>KPC-3<\/jats:sub>\n                    <jats:italic>, bla<\/jats:italic>\n                    <jats:sub>CTX-M-9<\/jats:sub>\n                    <jats:italic>,<\/jats:italic>\n                    and\n                    <jats:italic>mcr-9<\/jats:italic>\n                    , were frequently linked to MGEs.\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    alleles were mobilized by Tn\n                    <jats:italic>4401<\/jats:italic>\n                    -like elements, while\n                    <jats:italic>mcr-9<\/jats:italic>\n                    occurred either within IncHI2\/IncHI2A plasmids or integrated into chromosomal contexts, underscoring diverse mobilization routes. In contrast, the virulome was comparatively conserved, dominated by motility, chemotaxis, and siderophore systems, unlike pathogenic\n                    <jats:italic>Enterobacterales<\/jats:italic>\n                    that carry broad MGE-associated virulence factors. Co-occurrence analyses showed modular independence between resistance and virulence, with limited overlaps shaped by ecological origins, suggesting that resistome content may adapt to distinctive environments. Collectively, these findings establish\n                    <jats:italic>P. vulneris<\/jats:italic>\n                    as a reservoir and conduit of last-resort resistance genes, reinforcing its relevance for One Health surveillance and highlighting the urgent need for its systematic inclusion in global antimicrobial resistance monitoring frameworks.\n                  <\/jats:p>","DOI":"10.1007\/s00284-026-04948-5","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:50:15Z","timestamp":1779209415000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["One Health Genomic Perspective on Pseudescherichia vulneris: A Neglected Reservoir of Last-Resort Resistance Genes"],"prefix":"10.1007","volume":"83","author":[{"given":"Anelise S.","family":"Ballaben","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia M.","family":"Cabrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro M.","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mick","family":"Chandler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro M.","family":"Varani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"4948_CR1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00162-24","author":"A Aguilar-Vera","year":"2024","unstructured":"Aguilar-Vera A et al (2024) Acinetobacter junii\u202f: an emerging One Health pathogen. mSphere. https:\/\/doi.org\/10.1128\/msphere.00162-24","journal-title":"mSphere"},{"issue":"1D","key":"4948_CR2","doi-asserted-by":"publisher","first-page":"D690","DOI":"10.1093\/nar\/gkac920","volume":"51","author":"BP Alcock","year":"2023","unstructured":"Alcock BP et al (2023) CARD 2023: expanded curation, support for machine learning, and resistome prediction at the comprehensive antibiotic resistance database. 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