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To address this limitation, atmospheric and room temperature plasma (ARTP) mutagenesis combined with a microbial microdroplet culture (MMC) high-throughput screening system was adopted to screen high salt-tolerant\n                    <jats:italic>B. megaterium<\/jats:italic>\n                    mutant followed by multi-omics characterization. Using WT\n                    <jats:italic>B. megaterium<\/jats:italic>\n                    LD (CGMCC No.21828) as the original strain, a mutant library was constructed via optimized ARTP treatment, and the elite mutant SLD48 was isolated under dynamically increasing salt gradients. Compared with the wild type, SLD48 possesses a larger genome of 6,252,538\u00a0bp and acquires 28 extra functional genes including\n                    <jats:italic>spoIVCA<\/jats:italic>\n                    and\n                    <jats:italic>pJ_gene0003<\/jats:italic>\n                    . Intracellular osmoprotectants (proline, glycine betaine) and antioxidant \u03b1-tocopherol were markedly accumulated in SLD48. moreover, exogenous supplementation of these substances in the culture medium can effectively alleviate the damage caused by salt stress to the cells. In pot trials with saline\u2013alkali soil, the rhizosphere colonization of SLD48 reached 3.52\u2009\u00d7\u200910\u2075 CFU\/g (2.46-fold of LD), and maize shoot fresh weight increased by 40.5% after inoculation. Collectively, this work confirms that mutant SLD48 is a promising microbial resource for saline\u2013alkali soil improvement and crop yield promotion.\n                  <\/jats:p>","DOI":"10.1007\/s00284-026-05077-9","type":"journal-article","created":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T18:45:06Z","timestamp":1785696306000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Novel ARTP-derived Bacillus megaterium Mutant with Enhanced Salt Tolerance and Plant Growth Promotion in Saline\u2013alkali Soil"],"prefix":"10.1007","volume":"83","author":[{"given":"Min","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PengFei","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XinJie","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"QiLi","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZhenXing","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JiMin","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HongShun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JunPing","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YanQing","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ShuDong","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XianShun","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZiHao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7544-8040","authenticated-orcid":false,"given":"Yi","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YongJun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,2]]},"reference":[{"issue":"1","key":"5077_CR1","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/s11084-025-09688-3","volume":"55","author":"DB Shelke","year":"2025","unstructured":"Shelke DB et al (2025) Synergistic approaches in halophyte-microbe interactions: mitigating soil salinity and industrial contaminants for sustainable agriculture. 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