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In this study,\n                    <jats:italic>P. albidus<\/jats:italic>\n                    was initially cultivated on PDA agar supplemented with yeast extract (YE) or ammonium sulfate (AS) at 0.5\u20132 gN\/L for nitrogen source screening. On agar medium, YE reduced the lag phase and accelerated plate colonization, reaching full plate coverage in 8\u20139 days compared to 11\u201312 days for AS. Based on these results, the selected condition was subsequently applied to submerged cultivation in which the mycelium grew as spherical pellets and reached 12.4\u00a0g\/L after 12 days, with 23.8% protein, 26% fiber, and 39% carbohydrates. Chemical score analysis indicated that most essential amino acids (EAAs) exceeded recommended levels; however, sulfur-containing amino acids (methionine\u2009+\u2009cystine) were limiting (0.6). Threonine showed the highest score (2.3), while lysine (1.2) and tryptophan (1.1) also scored above 1.0. Non-essential amino acids such as glutamic (145\u00a0mg\/g) and aspartic acid (102\u00a0mg\/g) predominated. These results highlight the nutritional potential of\n                    <jats:italic>P. albidus<\/jats:italic>\n                    mycelium as a novel protein source and support its further investigation for future food applications, while additional studies on protein digestibility and cultivation optimization are needed to further improve amino acid balance and protein quality.\n                  <\/jats:p>","DOI":"10.1007\/s00284-026-05119-2","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T11:25:21Z","timestamp":1787743521000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Nitrogen Source Screening on Agar Medium Followed by Submerged Cultivation of Pleurotus albidus Mycelium for Nutritional and Amino Acid Characterization"],"prefix":"10.1007","volume":"83","author":[{"given":"Eduarda Monteiro Martins","family":"Leit\u00e3o","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ac\u00e1cio A. 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