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Pathogenicity assays confirmed the two strains isolated from foliage to be non-pathogenic, while the strain 21TX0197\n                    <jats:sup>T<\/jats:sup>\n                    was found to be pathogenic. Phylogenetic analysis using the 16\u00a0S rRNA gene revealed the closest resemblance of 20TX0008\n                    <jats:sup>T<\/jats:sup>\n                    , 20TX0035\n                    <jats:sup>T<\/jats:sup>\n                    , and 21TX0197\n                    <jats:sup>T<\/jats:sup>\n                    to the genera\n                    <jats:italic>Curtobacterium<\/jats:italic>\n                    ,\n                    <jats:italic>Falsirhodobacter<\/jats:italic>\n                    , and\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    , respectively. For the respective genus, strains 20TX0008\n                    <jats:sup>T<\/jats:sup>\n                    showed the greatest 16\u00a0S rRNA sequence similarity with\n                    <jats:italic>Curtobacterium caesinilyticum<\/jats:italic>\n                    GDMCC 1.2667\n                    <jats:sup>T<\/jats:sup>\n                    (99.7%), 20TX0035\n                    <jats:sup>T<\/jats:sup>\n                    with\n                    <jats:italic>Falsirhodobacter deserti<\/jats:italic>\n                    W402\n                    <jats:sup>T<\/jats:sup>\n                    (99.1%), and 21TX0197\n                    <jats:sup>T<\/jats:sup>\n                    with\n                    <jats:italic>Pseudomonas uvaldensis<\/jats:italic>\n                    20TX0172\n                    <jats:sup>T<\/jats:sup>\n                    (99.4%). In the phylogenetic trees based on whole genome sequences, all three strains formed a distinct, well-supported branch that separated them from their closely related species within the same genus. The genomic similarity, based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), revealed that the novel strains had values below the species delineation thresholds (ANI\u2009&lt;\u200995% and dDDH\u2009&lt;\u200970%), confirming the novelty of all these strains. Based on genomic and phenotypic characteristics, we propose three novel species,\n                    <jats:italic>Curtobacterium uvaldense<\/jats:italic>\n                    sp. nov. (type strain 20TX0008\n                    <jats:sup>T<\/jats:sup>\n                    =LMG 33207\n                    <jats:sup>T<\/jats:sup>\n                    =NCIMB 15480\n                    <jats:sup>T<\/jats:sup>\n                    ),\n                    <jats:italic>Falsirhodobacter allii<\/jats:italic>\n                    sp. nov. (type strain 20TX0035\n                    <jats:sup>T<\/jats:sup>\n                    =LMG 33208\n                    <jats:sup>T<\/jats:sup>\n                    =NCIMB 15477\n                    <jats:sup>T<\/jats:sup>\n                    ), and\n                    <jats:italic>Pseudomonas texasensis<\/jats:italic>\n                    sp. nov. (type strain 21TX0197\n                    <jats:sup>T<\/jats:sup>\n                    =LMG 33210\n                    <jats:sup>T<\/jats:sup>\n                    =NCIMB 15479\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1007\/s00284-026-05144-1","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:45:03Z","timestamp":1788961503000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Curtobacterium uvaldense sp. nov., Falsirhodobacter allii sp. nov., Pseudomonas texasensis sp. nov., Three Novel Species Isolated from Onions in Texas, USA"],"prefix":"10.1007","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1818-8753","authenticated-orcid":false,"given":"Manzeal","family":"Khanal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bed Prakash","family":"Bhatta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakhvir","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subas","family":"Malla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"issue":"2","key":"5144_CR1","doi-asserted-by":"publisher","first-page":"kuab076","DOI":"10.1093\/jimb\/kuab076","volume":"49","author":"A Shi","year":"2022","unstructured":"Shi A, Fan F, Broach JR (2022) Microbial adaptive evolution. 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These tools were employed for writing refinement, figure formatting, and rapid summarization of relevant studies. All scientific claims and interpretations were independently verified against primary sources, and full responsibility for the content rests solely with the authors.","order":1,"name":"Ethics","label":"Authors\u2019 statements","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The GenBank accession numbers for the genomes of strains 20TX0008\n                      T\n                      , 20TX0035\n                      T\n                      , and 21TX0197\n                      T\n                      are JAQMHU000000000, JAQMHV000000000, and JAQMHS000000000, respectively. The GenBank accession numbers for the 16S rRNA sequences of strains 20TX0008\n                      T\n                      , 20TX0035\n                      T\n                      , and 21TX0197\n                      T\n                      are ON255719, ON255745, and OP325568, respectively.","order":3,"name":"Ethics","label":"Repositories","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"559"}}