{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:29:20Z","timestamp":1709166560053},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Johann Wolfgang Goethe-Universit\u00e4t, Frankfurt am Main"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pathologie"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:sec>\n                <jats:title>Hintergrund<\/jats:title>\n                <jats:p>Die MASLD (metabolische Dysfunktion-assoziierte steatotische Lebererkrankung, oder nichtalkoholische Fettlebererkrankung [NAFLD]) ist eine h\u00e4ufige Erkrankung, deren Diagnose auf der lichtmikroskopischen Auswertung von Leberbiopsien basiert. Diese unterliegt jedoch einer gro\u00dfen Interbetrachtervariabilit\u00e4t (IBV), die durch Hinzunahme von automatisierten Methoden verringert werden kann.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Ziel der Arbeit<\/jats:title>\n                <jats:p>Ein Gro\u00dfteil der bestehenden computerbasierenden Methoden reflektiert nicht das, was in der Realit\u00e4t vom Pathologen bewertet wird. Ziel ist es, aufzuzeigen, wie diese Unterschiede die Vorhersage des Verfettungsgrads (VG) beeinflussen. Zus\u00e4tzlich erschwert die IBV die Validierung von Algorithmen.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Material und Methoden<\/jats:title>\n                <jats:p>Insgesamt 40\u00a0Gewebeschnitte wurden automatisch mit Bildanalysemethoden zur Fett\u2011, Zellkern- und Fibroseerkennung ausgewertet. Die Daten wurden verwendet, um den VG zu berechnen. Die IBV bei der Quantifizierung des VG wurde dabei an 18\u00a0Gewebeschnitten durch unterschiedliche Pathologen analysiert.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Ergebnisse<\/jats:title>\n                <jats:p>Fl\u00e4chenbasierte Ans\u00e4tze erzielten st\u00e4rkere Korrelationen als zellkernbasierte Methoden (\u2300 Spearman-Rho [\u03c1]\u202f=\u20090,92 vs. 0,79). Die Hinzunahme von Informationen zur Gewebekomposition verringerte f\u00fcr fl\u00e4chenbasierte und zellkernbasierte Methoden den durchschnittlichen absoluten Vorhersagefehler um 0,5\u202f% bzw. 2,2\u202f%. Unser finaler fl\u00e4chenbasierter Algorithmus, der Informationen zum Gewebeaufbau integriert, erreichte eine hohe Genauigkeit (80\u202f%) und starke Korrelation (\u2300 \u03c1\u202f=\u20090,94) mit der manuellen Auswertung.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Diskussion<\/jats:title>\n                <jats:p>Die automatische und deterministische Bestimmung des VG l\u00e4sst sich durch die Integration von Informationen der Gewebekomposition verbessern und kann dazu dienen, den Einfluss der IBV zu verringern.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00292-024-01298-6","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T08:02:58Z","timestamp":1708502578000},"page":"115-123","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Herausforderungen der Automation bei der quantitativen Auswertung von Leberbiopsien","Challenges of automation in quantitative evaluation of liver biopsies"],"prefix":"10.1007","volume":"45","author":[{"given":"Jessica","family":"Darling","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nada","family":"Abedin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul K.","family":"Ziegler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen","family":"Gretser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Walczak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"Barreiros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Falko","family":"Schulze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henning","family":"Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Wild","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Flinner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,2,21]]},"reference":[{"key":"1298_CR1","doi-asserted-by":"publisher","first-page":"447","DOI":"10.3390\/app10020447","volume":"10","author":"A Arjmand","year":"2020","unstructured":"Arjmand\u00a0A, Tsipouras\u00a0MG, Tzallas\u00a0AT et\u00a0al (2020) Quantification of liver fibrosis\u2014a comparative study. 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Finanzierung, Bereitstellung von Studienmaterialien, medizinisches Schreiben, Artikelbearbeitungsgeb\u00fchren usw.): Mildred-Scheel-Nachwuchszentrum (MSNZ) Frankfurt, Promotionsstipendium. <i>In den letzten 36\u00a0Monaten:<\/i> Unterst\u00fctzung bei der Teilnahme an Meetings und\/oder Reisen: Mildred-Scheel-Nachwuchszentrum (MSNZ) Frankfurt, Goethe-Universit\u00e4t, Dr. Senckenbergisches Institut. N.\u00a0Abedin<i>: In den letzten 36\u00a0Monaten:<\/i> Zusch\u00fcsse oder Vertr\u00e4ge: Patenschaftsmodell der Frankfurter Forschungsf\u00f6rderung; Bezahlung oder Honorare f\u00fcr Vortr\u00e4ge, Pr\u00e4sentationen, Referentenb\u00fcros, Manuskripterstellung oder Bildungsveranstaltungen: Abbvie; Unterst\u00fctzung bei der Teilnahme an Meetings und\/oder Reisen: Abbvie. N.\u00a0Flinner: <i>Zeitlich unbegrenzt:<\/i> jegliche Unterst\u00fctzung f\u00fcr das vorliegende Manuskript (z.\u202fB. Finanzierung, Bereitstellung von Studienmaterialien, medizinisches Schreiben, Artikelbearbeitungsgeb\u00fchren usw.): MSNZ Frankfurt. P.\u00a0J.\u00a0Wild: <i>In den letzten 36\u00a0Monaten:<\/i> Zusch\u00fcsse oder Vertr\u00e4ge: AstraZeneca, Thermo Fisher Scientific; Bezahlung oder Honorare f\u00fcr Vortr\u00e4ge, Pr\u00e4sentationen, Referentenb\u00fcros, Manuskripterstellung oder Bildungsveranstaltungen: Bayer, Janssen-Cilag, Novartis, Roche, MSD, Astellas Pharma, Bristol-Myers Squibb, Hedera Dx, Thermo Fisher Scientific, Molecular Health, Sophia Genetics, Qiagen, Eli Lilly, Myriad, AstraZeneca. H.\u00a0Reis: <i>In den letzten 36\u00a0Monaten:<\/i> Zusch\u00fcsse oder Vertr\u00e4ge: Bristol-Myers Squibb; Beratungshonorare: Roche, Bristol-Myers Squibb; Bezahlung oder Honorare f\u00fcr Vortr\u00e4ge, Pr\u00e4sentationen, Referentenb\u00fcros, Manuskripterstellung oder Bildungsveranstaltungen: Roche, Bristol-Myers Squibb, Janssen-Cilag, Novartis, AstraZeneca, MCI, CHOP GmbH, Sanofi, Boehringer Ingelheim, GlaxoSmithKline, Merck, Diaceutics; Unterst\u00fctzung bei der Teilnahme an Meetings und\/oder Reisen: Philips, Roche, Bristol-Myers Squibb; Mitarbeit in einem Data Safety Monitoring Board oder Beirat: Adboards: Roche, Bristol-Myers Squibb. P.\u00a0K.\u00a0Ziegler, S.\u00a0Gretser, B.\u00a0Walczak, A.\u00a0P.\u00a0Barreiros und F.\u00a0Schulze geben an, dass kein Interessenkonflikt besteht.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Interessenkonflikt"}},{"value":"Die Studie wurde von der Ethikkommission durch das UCT Frankfurt genehmigt (UCT-47-2020).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Interessenkonflikt"}}]}}