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Insbesondere bei heterogen zusammengesetzten Geweben wie dem Pankreas oder bei komplexen Gewebspathologien erschwert die Beschr\u00e4nkung auf zweidimensionale Schnittbilder die ubiquit\u00e4re Erfassung morphologischer Merkmale.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Ziel der Arbeit (Fragestellung)<\/jats:title>\n                    <jats:p>Ziel dieser Studie ist es, das Potenzial der synchrotronbasierten Phasenkontrastbildgebung (SR\u00b5CT) f\u00fcr die hochaufl\u00f6sende, dreidimensionale Visualisierung verschiedener Pankreasgewebe zu demonstrieren. Anhand dreier paradigmatischer Fallbeispiele werden morphologische Parameter volumetrisch erfasst und mit korrespondierenden immunhistochemischen Markerprofilen korreliert.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material und Methoden<\/jats:title>\n                    <jats:p>Gewebestanzen aus formalinfixierten, paraffineingebetteten Bl\u00f6cken von humanen Pankreasgewebeproben wurden mittels SR\u00b5CT volumetrisch erfasst. Das untersuchte Probenmaterial wurde als Microarrays weiterverarbeitet. Konsekutive Schnitte und immunhistochemische F\u00e4rbungen wurden mit den 3D-Datens\u00e4tzen korreliert.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Ergebnisse<\/jats:title>\n                    <jats:p>Die Bildgebung erm\u00f6glichte die differenzierte r\u00e4umliche Darstellung funktioneller Kompartimente und neoplastischer Infiltrationsmuster. Nichtneoplastisches Gewebe zeigte klar abgegrenzte Kompartimente. Ein gut differenzierter neuroendokriner Tumor pr\u00e4sentierte trabekul\u00e4re Binnenstrukturen. Das duktale Adenokarzinom zeigte ein infiltratives Wachstumsmuster mit diffuser, heterogener Architektur, irregul\u00e4ren Gangformationen und Stromadesmoplasie. Die virtuelle Schnittf\u00fchrung erm\u00f6glichte die Analyse in jeder Raumrichtung. Durch Korrelation mit immunhistochemischen Markerprofilen konnten morphofunktionelle Merkmale validiert werden.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Schlussfolgerung<\/jats:title>\n                    <jats:p>Die SR\u00b5CT ist eine hochsensitive Methode, die nichtinvasiv und ohne F\u00e4rbung dreidimensionale Einsichten in die Gewebearchitektur des Pankreas unter Nutzung archivierter Paraffinbl\u00f6cke erlaubt. Die Methode bietet neue Perspektiven f\u00fcr Forschung, Lehre und potenziell erweiterte Spezialdiagnostik.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00292-025-01533-8","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T10:25:35Z","timestamp":1767867935000},"page":"136-143","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Virtuelle Histopathologie des Pankreas: 3D-Einblicke mittels synchrotronbasierter Bildgebung","Virtual histopathology of the pancreas: 3D insights using synchrotron-based imaging"],"prefix":"10.1007","volume":"47","author":[{"given":"Matthias Martin","family":"Gaida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas","family":"Hessel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline Victoria","family":"Schimmel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klara","family":"Schulze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas","family":"Albers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Duke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Loos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3964-3527","authenticated-orcid":false,"given":"Gabriel Alexander","family":"Salg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"1533_CR1","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.medj.2022.11.009","volume":"4","author":"AL Kiemen","year":"2023","unstructured":"Kiemen\u00a0AL et\u00a0al (2023) Tissue clearing and 3D reconstruction of digitized, serially sectioned slides provide novel insights into pancreatic cancer. 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