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Recent studies suggest that CBD consumption may influence the gut microbiome, impacting overall health. The mechanism of CBD action on the intestinal microbiome may be linked to the endocannabinoid system, which plays a role in the normalization of the functions of the immune system. Additionally, CBD may have a direct influence on the gut by interacting with the intestinal microflora, regulating intestinal permeability, modulating immune responses, and affecting the gut-brain axis communication through the vagal signaling pathway. A study was conducted to analyze how different doses of CBD injected intraperitoneal could alter the microbial composition of the duodenum contents in a mouse model. Obtained results suggest that the impact of CBD on the intestine microbiome may be dosage-dependent. Statistical analysis revealed significant differences compared to the control group in the 0.2\u00a0mg\/kg CBD and 20\u00a0mg\/kg CBD dose groups for the genera Campilobacteriota, Firmicutes, Proteobacteria, and, specifically in the 0.2\u00a0mg CBD group, Cyanobacteria. In contrast, the 10\u00a0mg CBD dose group showed no statistically significant differences relative to the control. The Firmicutes phylum had the highest relative abundance in all groups, wherein its share was lower in the CBD-treated groups than in the control group.\n                    <jats:italic>Lachnospiraceae<\/jats:italic>\n                    _\n                    <jats:italic>NK4A136<\/jats:italic>\n                    _\n                    <jats:italic>group<\/jats:italic>\n                    ,\n                    <jats:italic>Muribaculaceae<\/jats:italic>\n                    , and\n                    <jats:italic>Akkermansia<\/jats:italic>\n                    were predominant, with\n                    <jats:italic>Lachnospiraceae_NK4A136_group<\/jats:italic>\n                    decreasing and\n                    <jats:italic>Akkermansia<\/jats:italic>\n                    increasing in relative abundance in CBD-treated groups. Of the genera that showed statistically significant differences between groups, most belonged to the phylum Firmicutes, including the dominant\n                    <jats:italic>Lachnospiraceae_NK4A136_group<\/jats:italic>\n                    . These findings suggest that intraperitoneal CBD administration induces alterations in the duodenal microbiome, particularly affecting the\n                    <jats:italic>Lachnospiraceae_NK4A136_group<\/jats:italic>\n                    and the\n                    <jats:italic>Akkermansia<\/jats:italic>\n                    genera.\n                  <\/jats:p>","DOI":"10.1007\/s00294-025-01327-8","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T17:18:08Z","timestamp":1759252688000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of intraperitoneal cannabidiol (CBD) injection on intestine microbiome profile in a mouse model"],"prefix":"10.1007","volume":"71","author":[{"given":"Igor","family":"Jasielczuk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Oc\u0142o\u0144","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"\u017burowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Szmato\u0142a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Mizera-Szpilka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Gurgul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,9,30]]},"reference":[{"key":"1327_CR1","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1007\/s11095-019-2745-x","volume":"37","author":"A Al Shoyaib","year":"2020","unstructured":"Al Shoyaib A, Archie SR, Karamyan VT (2020) Intraperitoneal route of drug administration: should it be used in experimental animal studies? 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