{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:17:05Z","timestamp":1787159825232,"version":"build-2736575974"},"reference-count":61,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["PON03PE_00203_1"],"award-info":[{"award-number":["PON03PE_00203_1"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004462","name":"Consiglio Nazionale Delle Ricerche","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004462","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Plant Cell Rep"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Key message<\/jats:title>\n                    <jats:p>\n                      Gene expression analyses reveal tissue-specific and temporally regulated networks driving\n                      <jats:italic>Posidonia oceanica<\/jats:italic>\n                      development, and identify key hub genes that coordinate root, leaf, and seed maturation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Seagrasses are marine angiosperms that form extensive underwater meadows, providing habitat, stabilising sediments, storing carbon, and protecting coastlines.\n                      <jats:italic>Posidonia oceanica<\/jats:italic>\n                      is the endemic foundation seagrass of the Mediterranean Sea, yet its meadows are rapidly declining. Despite its ecological importance, the molecular basis of\n                      <jats:italic>P. oceanica<\/jats:italic>\n                      development is still poorly understood. Here, we analysed gene expression in roots, leaves, and seeds across four developmental stages, revealing strong tissue-specific patterns and temporally regulated expression dynamics. Leaves exhibited active regulation of photosynthesis-related processes, while roots were enriched in pathways related to carbohydrate metabolism and cell wall biogenesis, supporting primary root growth and anchoring. The seeds retained metabolic activity, with glycolytic enzymes indicating readiness for germination. Temporal analyses identified a major transcriptional shift, with distinct gene sets sequentially activated during early establishment and late maturation in tissues. Weighted Gene Co-expression Network Analysis (WGCNA) identified modules strongly associated with specific tissues and developmental transitions, highlighting key hub genes involved in photosynthesis, metabolism, cell wall remodelling, and protein synthesis. Together, these results reveal complex, temporally coordinated regulatory networks underlying\n                      <jats:italic>P. oceanica<\/jats:italic>\n                      development\n                      <jats:italic>.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00299-026-03887-6","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T16:15:32Z","timestamp":1782490532000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gene expression landscapes driving early life stages of the keystone seagrass Posidonia oceanica"],"prefix":"10.1007","volume":"45","author":[{"given":"Gianmarco","family":"Valenti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Sutera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6005-5079","authenticated-orcid":false,"given":"Emanuela","family":"Dattolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4822-7971","authenticated-orcid":false,"given":"Francesco","family":"Cosenza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7700-9527","authenticated-orcid":false,"given":"Francesco","family":"Carimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6179-468X","authenticated-orcid":false,"given":"Gabriele","family":"Procaccini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1356-2881","authenticated-orcid":false,"given":"Francesco","family":"Mercati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4290-9733","authenticated-orcid":false,"given":"Guglielmo","family":"Puccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8987-6599","authenticated-orcid":false,"given":"Roberto","family":"De Michele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"3887_CR1","doi-asserted-by":"publisher","first-page":"99","DOI":"10.3354\/MEPS08104","volume":"388","author":"E Balestri","year":"2009","unstructured":"Balestri E, Gobert S, Lepoint G, Lardicci C (2009) Seed nutrient content and nutritional status of Posidonia oceanica seedlings in the northwestern Mediterranean Sea. Mar Ecol Prog Ser 388:99\u2013109. https:\/\/doi.org\/10.3354\/MEPS08104","journal-title":"Mar Ecol Prog Ser"},{"key":"3887_CR2","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/j.aquabot.2005.04.006","volume":"82","author":"M Belzunce","year":"2005","unstructured":"Belzunce M, Navarro RM, Rapoport HF (2005) Seed and early plantlet structure of the Mediterranean seagrass Posidonia oceanica. Aquat Bot 82:269\u2013283. https:\/\/doi.org\/10.1016\/j.aquabot.2005.04.006","journal-title":"Aquat Bot"},{"key":"3887_CR3","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/j.aquabot.2007.10.008","volume":"88","author":"M Belzunce","year":"2008","unstructured":"Belzunce M, Navarro RM, Rapoport HF (2008) Posidonia oceanica seedling root structure and development. Aquat Bot 88:203\u2013210. https:\/\/doi.org\/10.1016\/j.aquabot.2007.10.008","journal-title":"Aquat Bot"},{"key":"3887_CR4","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/BIOINFORMATICS\/BTU170","volume":"30","author":"AM Bolger","year":"2014","unstructured":"Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114\u20132120. https:\/\/doi.org\/10.1093\/BIOINFORMATICS\/BTU170","journal-title":"Bioinformatics"},{"key":"3887_CR5","doi-asserted-by":"publisher","first-page":"5809","DOI":"10.1093\/JXB\/ERZ346","volume":"70","author":"GL Borghi","year":"2019","unstructured":"Borghi GL, Moraes TA, G\u00fcnther M et al (2019) Relationship between irradiance and levels of Calvin\u2013Benson cycle and other intermediates in the model eudicot Arabidopsis and the model monocot rice. J Exp Bot 70:5809. https:\/\/doi.org\/10.1093\/JXB\/ERZ346","journal-title":"J Exp Bot"},{"key":"3887_CR6","doi-asserted-by":"publisher","DOI":"10.3390\/W13081034","volume":"13","author":"CF Boudouresque","year":"2021","unstructured":"Boudouresque CF, Blanfun\u00e9 A, Pergent G, Thibaut T (2021) Restoration of seagrass meadows in the Mediterranean Sea: a critical review of effectiveness and ethical issues. Water 13:1034. https:\/\/doi.org\/10.3390\/W13081034","journal-title":"Water"},{"key":"3887_CR7","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1126\/SCIENCE.1146265","volume":"318","author":"SM Brady","year":"2007","unstructured":"Brady SM, Orlando DA, Lee JY et al (2007) A high-resolution root spatiotemporal map reveals dominant expression patterns. Science 318:801\u2013806. https:\/\/doi.org\/10.1126\/SCIENCE.1146265","journal-title":"Science"},{"key":"3887_CR8","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/J.PLAPHY.2016.07.019","volume":"108","author":"B Cai","year":"2016","unstructured":"Cai B, Li Q, Xu Y et al (2016) Genome-wide analysis of the fructose 1,6-bisphosphate aldolase (FBA) gene family and functional characterization of FBA7 in tomato. Plant Physiol Biochem 108:251\u2013265. https:\/\/doi.org\/10.1016\/J.PLAPHY.2016.07.019","journal-title":"Plant Physiol Biochem"},{"key":"3887_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1890\/ES13-00104.1","volume":"4","author":"D Celdran","year":"2013","unstructured":"Celdran D, Marin A (2013) Seed photosynthesis enhances Posidonia oceanica seedling growth seed collection. Ecosphere 4:1\u201311. https:\/\/doi.org\/10.1890\/ES13-00104.1","journal-title":"Ecosphere"},{"key":"3887_CR10","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0130015","volume":"10","author":"D Celdran","year":"2015","unstructured":"Celdran D, Lloret J, Verduin J et al (2015) Linking seed photosynthesis and evolution of the Australian and Mediterranean seagrass genus Posidonia. PLoS ONE 10:e0130015. https:\/\/doi.org\/10.1371\/JOURNAL.PONE.0130015","journal-title":"PLoS ONE"},{"key":"3887_CR11","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.70259","volume":"15","author":"D Crucitti","year":"2026","unstructured":"Crucitti D, Sutera A, Carimi F et al (2026) Microbial life Inside Posidonia seeds: beneficial endophytes and implications for marine plant health. Microbiolopen 15:e70259. https:\/\/doi.org\/10.1002\/mbo3.70259","journal-title":"Microbiolopen"},{"key":"3887_CR12","doi-asserted-by":"publisher","first-page":"195","DOI":"10.3389\/fpls.2013.00195","volume":"4","author":"E Dattolo","year":"2013","unstructured":"Dattolo E, Gu J, Bayer PE et al (2013) Acclimation to different depths by the marine angiosperm Posidonia oceanica: transcriptomic and proteomic profiles. Front Plant Sci 4:195. https:\/\/doi.org\/10.3389\/fpls.2013.00195","journal-title":"Front Plant Sci"},{"key":"3887_CR13","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1002\/ECE3.2731","volume":"7","author":"E Dattolo","year":"2017","unstructured":"Dattolo E, Mar\u00edn-Guirao L, Ruiz JM, Procaccini G (2017) Long-term acclimation to reciprocal light conditions suggests depth-related selection in the marine foundation species Posidonia oceanica. Ecol Evol 7:1148\u20131164. https:\/\/doi.org\/10.1002\/ECE3.2731","journal-title":"Ecol Evol"},{"key":"3887_CR14","doi-asserted-by":"publisher","first-page":"3191","DOI":"10.5194\/BG-14-3191-2017","volume":"14","author":"G De Falco","year":"2017","unstructured":"De Falco G, Molinaroli E, Conforti A et al (2017) Biogenic sediments from coastal ecosystems to beach-dune systems: Implications for the adaptation of mixed and carbonate beaches to future sea level rise. Biogeosciences 14:3191\u20133205. https:\/\/doi.org\/10.5194\/BG-14-3191-2017","journal-title":"Biogeosciences"},{"key":"3887_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/BS.ABR.2022.02.001","volume":"104","author":"JC del R\u00edo","year":"2022","unstructured":"del R\u00edo JC, Rencoret J, Guti\u00e9rrez A et al (2022) Unconventional lignin monomers\u2014extension of the lignin paradigm. Adv Bot Res 104:1\u201339. https:\/\/doi.org\/10.1016\/BS.ABR.2022.02.001","journal-title":"Adv Bot Res"},{"key":"3887_CR16","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/BIOINFORMATICS\/BTS635","volume":"29","author":"A Dobin","year":"2013","unstructured":"Dobin A, Davis CA, Schlesinger F et al (2013) STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29:15\u201321. https:\/\/doi.org\/10.1093\/BIOINFORMATICS\/BTS635","journal-title":"Bioinformatics"},{"key":"3887_CR17","doi-asserted-by":"publisher","DOI":"10.3389\/FMARS.2017.00013","volume":"4","author":"CM Duarte","year":"2017","unstructured":"Duarte CM, Krause-Jensen D (2017) Export from seagrass meadows contributes to marine carbon sequestration. Front Mar Sci 4:13. https:\/\/doi.org\/10.3389\/FMARS.2017.00013","journal-title":"Front Mar Sci"},{"key":"3887_CR18","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.margen.2017.05.006","volume":"35","author":"L Entrambasaguas","year":"2017","unstructured":"Entrambasaguas L, Jahnke M, Biffali E et al (2017) Tissue-specific transcriptomic profiling provides new insights into the reproductive ecology and biology of the iconic seagrass species Posidonia oceanica. Mar Genomics 35:51\u201361. https:\/\/doi.org\/10.1016\/j.margen.2017.05.006","journal-title":"Mar Genomics"},{"key":"3887_CR19","doi-asserted-by":"publisher","DOI":"10.1002\/IMT2.43","volume":"1","author":"Z Gu","year":"2022","unstructured":"Gu Z (2022) Complex heatmap visualization. iMeta 1:e43. https:\/\/doi.org\/10.1002\/IMT2.43","journal-title":"iMeta"},{"key":"3887_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0207345","volume":"13","author":"L Guerrero-Meseguer","year":"2018","unstructured":"Guerrero-Meseguer L, Sanz-L\u00e1zaro C, Mar\u00edn A (2018) Understanding the sexual recruitment of one of the oldest and largest organisms on Earth, the seagrass Posidonia oceanica. PLoS ONE 13:e0207345. https:\/\/doi.org\/10.1371\/JOURNAL.PONE.0207345","journal-title":"PLoS ONE"},{"key":"3887_CR21","doi-asserted-by":"publisher","DOI":"10.3390\/PLANTS12173155","author":"M Ismael","year":"2023","unstructured":"Ismael M, Charras Q, Leschevin M et al (2023) Seasonal variation in cell wall composition and carbohydrate metabolism in the seagrass Posidonia oceanica growing at different depths. Plants. https:\/\/doi.org\/10.3390\/PLANTS12173155","journal-title":"Plants"},{"key":"3887_CR22","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1104\/PP.113.234294","volume":"165","author":"MP Joseph","year":"2014","unstructured":"Joseph MP, Papdi C, Kozma-Bogn\u00e1r L et al (2014) The arabidopsis ZINC FINGER PROTEIN3 Interferes with abscisic acid and light signaling in seed germination and plant development. Plant Physiol 165:1203\u20131220. https:\/\/doi.org\/10.1104\/PP.113.234294","journal-title":"Plant Physiol"},{"key":"3887_CR23","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1016\/J.JMB.2015.11.006","volume":"428","author":"M Kanehisa","year":"2016","unstructured":"Kanehisa M, Sato Y, Morishima K (2016) BlastKOALA and GhostKOALA: KEGG tools for functional characterization of genome and metagenome sequences. J Mol Biol 428:726\u2013731. https:\/\/doi.org\/10.1016\/J.JMB.2015.11.006","journal-title":"J Mol Biol"},{"key":"3887_CR24","doi-asserted-by":"publisher","DOI":"10.3390\/GENES12101620","author":"MK Koramutla","year":"2021","unstructured":"Koramutla MK, Negi M, Ayele BT (2021) Roles of glutathione in mediating abscisic acid signaling and Its regulation of seed dormancy and drought tolerance. Genes. https:\/\/doi.org\/10.3390\/GENES12101620","journal-title":"Genes"},{"key":"3887_CR25","doi-asserted-by":"publisher","first-page":"2209","DOI":"10.1111\/PCE.1321","volume":"41","author":"H Kudapa","year":"2018","unstructured":"Kudapa H, Garg V, Chitikineni A, Varshney RK (2018) The RNA-Seq-based high resolution gene expression atlas of chickpea (Cicer arietinum L.) reveals dynamic spatio-temporal changes associated with growth and development. Plant Cell Environ 41:2209\u20132225. https:\/\/doi.org\/10.1111\/PCE.1321","journal-title":"Plant Cell Environ"},{"key":"3887_CR26","doi-asserted-by":"publisher","DOI":"10.3389\/FPLS.2016.02023","volume":"7","author":"M Kumar","year":"2017","unstructured":"Kumar M, Padula MP, Davey P et al (2017) Proteome analysis reveals extensive light stress-response reprogramming in the seagrass Zostera muelleri (Alismatales, Zosteraceae) metabolism. Front Plant Sci 7:238280. https:\/\/doi.org\/10.3389\/FPLS.2016.02023","journal-title":"Front Plant Sci"},{"key":"3887_CR27","doi-asserted-by":"publisher","first-page":"80399","DOI":"10.3389\/FPLS.2014.00090","volume":"5","author":"L Lei","year":"2014","unstructured":"Lei L, Li S, Bashline L, Gu Y (2014) Dissecting the molecular mechanism underlying the intimate relationship between cellulose microfibrils and cortical microtubules. Front Plant Sci 5:80399. https:\/\/doi.org\/10.3389\/FPLS.2014.00090","journal-title":"Front Plant Sci"},{"key":"3887_CR28","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1093\/BIOINFORMATICS\/BTT656","volume":"30","author":"Y Liao","year":"2014","unstructured":"Liao Y, Smyth GK, Shi W (2014) Featurecounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics 30:923\u2013930. https:\/\/doi.org\/10.1093\/BIOINFORMATICS\/BTT656","journal-title":"Bioinformatics"},{"issue":"12","key":"3887_CR29","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15(12):550. https:\/\/doi.org\/10.1186\/s13059-014-0550-8","journal-title":"Genome Biol"},{"key":"3887_CR30","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1038\/S41477-023-01608-5","volume":"10","author":"X Ma","year":"2024","unstructured":"Ma X, Vanneste S, Chang J et al (2024a) Seagrass genomes reveal ancient polyploidy and adaptations to the marine environment. Nat Plants 10:240\u2013255. https:\/\/doi.org\/10.1038\/S41477-023-01608-5","journal-title":"Nat Plants"},{"key":"3887_CR31","doi-asserted-by":"publisher","DOI":"10.3390\/plants13213051","author":"Y Ma","year":"2024","unstructured":"Ma Y, Zhang Y, Xu J et al (2024b) Research on the mechanisms of phytohormone signaling in regulating root development. Plants. https:\/\/doi.org\/10.3390\/plants13213051","journal-title":"Plants"},{"key":"3887_CR32","doi-asserted-by":"publisher","first-page":"2486","DOI":"10.1111\/MEC.15089","volume":"28","author":"L Mar\u00edn-Guirao","year":"2019","unstructured":"Mar\u00edn-Guirao L, Entrambasaguas L, Ruiz JM, Procaccini G (2019) Heat-stress induced flowering can be a potential adaptive response to ocean warming for the iconic seagrass Posidonia oceanica. Mol Ecol 28:2486\u20132501. https:\/\/doi.org\/10.1111\/MEC.15089","journal-title":"Mol Ecol"},{"key":"3887_CR33","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0163091","volume":"11","author":"L Mtwana Nordlund","year":"2016","unstructured":"Mtwana Nordlund L, Koch EW, Barbier EB, Creed JC (2016) Seagrass ecosystem services and their variability across genera and geographical regions. PLoS ONE 11:e0163091. https:\/\/doi.org\/10.1371\/JOURNAL.PONE.0163091","journal-title":"PLoS ONE"},{"key":"3887_CR34","doi-asserted-by":"publisher","first-page":"277","DOI":"10.3354\/meps200277","volume":"200","author":"RJ Orth","year":"2000","unstructured":"Orth RJ, Harwell M, Bailey E et al (2000) A review of issues in seagrass seed dormancy and germination: implications for conservation and restoration. Mar Ecol Prog Ser 200:277. https:\/\/doi.org\/10.3354\/meps200277","journal-title":"Mar Ecol Prog Ser"},{"key":"3887_CR35","doi-asserted-by":"publisher","DOI":"10.1016\/J.ENVPOL.2022.119077","volume":"303","author":"J Pazzaglia","year":"2022","unstructured":"Pazzaglia J, Santill\u00e1n-Sarmiento A, Ruocco M et al (2022) Local environment modulates whole-transcriptome expression in the seagrass Posidonia oceanica under warming and nutrients excess. Environ Pollut 303:119077. https:\/\/doi.org\/10.1016\/J.ENVPOL.2022.119077","journal-title":"Environ Pollut"},{"key":"3887_CR37","doi-asserted-by":"publisher","DOI":"10.3389\/FPLS.2020.588754","volume":"11","author":"L Pfeifer","year":"2020","unstructured":"Pfeifer L, Classen B (2020) The cell wall of seagrasses: fascinating, peculiar and a blank canvas for future research. Front Plant Sci 11:588754. https:\/\/doi.org\/10.3389\/FPLS.2020.588754","journal-title":"Front Plant Sci"},{"key":"3887_CR38","doi-asserted-by":"publisher","first-page":"42890","DOI":"10.1038\/SREP42890","volume":"7","author":"G Procaccini","year":"2017","unstructured":"Procaccini G, Ruocco M, Mar\u00edn-Guirao L et al (2017) Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica. Sci Rep 7:42890. https:\/\/doi.org\/10.1038\/SREP42890","journal-title":"Sci Rep"},{"key":"3887_CR39","doi-asserted-by":"publisher","DOI":"10.1016\/J.SCITOTENV.2024.170326","volume":"916","author":"F Rodr\u00edguez-Rojas","year":"2024","unstructured":"Rodr\u00edguez-Rojas F, Navarrete C, R\u00e1mila C et al (2024) Transcriptomic profiles and diagnostic biomarkers in the Mediterranean seagrasses Posidonia oceanica and Cymodocea nodosa reveal mechanistic insights of adaptative strategies upon desalination brine stress. Sci Total Environ 916:170326. https:\/\/doi.org\/10.1016\/J.SCITOTENV.2024.170326","journal-title":"Sci Total Environ"},{"key":"3887_CR40","doi-asserted-by":"publisher","DOI":"10.3389\/FPLS.2019.01556","volume":"10","author":"M Ruocco","year":"2019","unstructured":"Ruocco M, De Luca P, Mar\u00edn-Guirao L, Procaccini G (2019) Differential leaf age-dependent thermal plasticity in the keystone seagrass Posidonia oceanica. Front Plant Sci 10:486501. https:\/\/doi.org\/10.3389\/FPLS.2019.01556","journal-title":"Front Plant Sci"},{"key":"3887_CR41","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1111\/1365-2745.13479","volume":"109","author":"M Ruocco","year":"2021","unstructured":"Ruocco M, Entrambasaguas L, Dattolo E et al (2021) A king and vassals\u2019 tale: molecular signatures of clonal integration in Posidonia oceanica under chronic light shortage. J Ecol 109:294\u2013312. https:\/\/doi.org\/10.1111\/1365-2745.13479","journal-title":"J Ecol"},{"key":"3887_CR42","doi-asserted-by":"publisher","DOI":"10.1016\/J.SCITOTENV.2023.162517","volume":"877","author":"A Santill\u00e1n-Sarmiento","year":"2023","unstructured":"Santill\u00e1n-Sarmiento A, Pazzaglia J, Ruocco M et al (2023) Gene co-expression network analysis for the selection of candidate early warning indicators of heat and nutrient stress in Posidonia oceanica. Sci Total Environ 877:162517. https:\/\/doi.org\/10.1016\/J.SCITOTENV.2023.162517","journal-title":"Sci Total Environ"},{"key":"3887_CR43","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1038\/ng1543","volume":"37","author":"M Schmid","year":"2005","unstructured":"Schmid M, Davison TS, Henz SR et al (2005) A gene expression map of Arabidopsis thaliana development. Nat Genet 37:501\u2013506. https:\/\/doi.org\/10.1038\/ng1543","journal-title":"Nat Genet"},{"key":"3887_CR44","doi-asserted-by":"publisher","first-page":"1988","DOI":"10.3390\/PLANTS11151988","volume":"11","author":"G Smolikova","year":"2022","unstructured":"Smolikova G, Medvedev S (2022) Seed-to-seedling transition: novel aspects. Plants 11:1988. https:\/\/doi.org\/10.3390\/PLANTS11151988","journal-title":"Plants"},{"key":"3887_CR45","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.01242","volume":"7","author":"M Somssich","year":"2016","unstructured":"Somssich M, Khan GA, Staffan SP (2016) Cell wall heterogeneity in root development of Arabidopsis. Front Plant Sci 7:205730. https:\/\/doi.org\/10.3389\/fpls.2016.01242","journal-title":"Front Plant Sci"},{"key":"3887_CR46","doi-asserted-by":"publisher","DOI":"10.3835\/plantgenome2015.04.0025","volume":"9","author":"SC Stelpflug","year":"2016","unstructured":"Stelpflug SC, Sekhon RS, Vaillancourt B et al (2016) An expanded maize gene expression atlas based on rna sequencing and its use to explore root development. Plant Genome 9:plantgenome2015.04.0025. https:\/\/doi.org\/10.3835\/plantgenome2015.04.0025","journal-title":"Plant Genome"},{"key":"3887_CR47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-024-56536-x","volume":"14","author":"A Sutera","year":"2024","unstructured":"Sutera A, Bonaviri C, Spinelli P et al (2024) Fruit encasing preserves the dispersal potential and viability of stranded Posidonia oceanica seeds. Sci Rep 14:1\u20138. https:\/\/doi.org\/10.1038\/s41598-024-56536-x","journal-title":"Sci Rep"},{"key":"3887_CR48","doi-asserted-by":"publisher","DOI":"10.1016\/J.BIOCON.2025.111385","volume":"310","author":"A Sutera","year":"2025","unstructured":"Sutera A, Spinelli P, Pacifico D et al (2025) Extended storage of Posidonia oceanica seeds and seedlings: a breakthrough for year-round seagrass restoration. Biol Conserv 310:111385. https:\/\/doi.org\/10.1016\/J.BIOCON.2025.111385","journal-title":"Biol Conserv"},{"key":"3887_CR49","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/srep12505","volume":"5","author":"L Telesca","year":"2015","unstructured":"Telesca L, Belluscio A, Criscoli A et al (2015) Seagrass meadows (Posidonia oceanica) distribution and trajectories of change. Sci Rep 5:1\u201314. https:\/\/doi.org\/10.1038\/srep12505","journal-title":"Sci Rep"},{"key":"3887_CR50","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.4161\/rna.26127","volume":"10","author":"NJ Tourasse","year":"2013","unstructured":"Tourasse NJ, Choquet Y, Vallon O (2013) PPR proteins of green algae. RNA Biol 10:1526\u20131542. https:\/\/doi.org\/10.4161\/rna.26127","journal-title":"RNA Biol"},{"issue":"17","key":"3887_CR51","doi-asserted-by":"publisher","first-page":"5129","DOI":"10.3390\/MOLECULES26175129","volume":"26","author":"RP Walker","year":"2021","unstructured":"Walker RP, Chen Z-H, Famiani F et al (2021) Gluconeogenesis in plants: a key interface between organic acid\/amino acid\/lipid and Sugar metabolism. Molecules 26(17):5129. https:\/\/doi.org\/10.3390\/MOLECULES26175129","journal-title":"Molecules"},{"key":"3887_CR52","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1002\/wics.147","volume":"3","author":"H Wickham","year":"2011","unstructured":"Wickham H (2011) ggplot2. Wiley Interdiscip Rev Comput Stat 3:180\u2013185. https:\/\/doi.org\/10.1002\/wics.147","journal-title":"Wiley Interdiscip Rev Comput Stat"},{"key":"3887_CR53","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.12688\/f1000research.15931.2","volume":"7","author":"SW Wingett","year":"2018","unstructured":"Wingett SW, Andrews S (2018) FastQ Screen: A tool for multi-genome mapping and quality control. F1000Res 7:1338. https:\/\/doi.org\/10.12688\/f1000research.15931.2","journal-title":"F1000Res"},{"issue":"1","key":"3887_CR54","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1186\/s13068-021-01946-6","volume":"14","author":"B Wu","year":"2021","unstructured":"Wu B, Sun M, Zhang H et al (2021) Transcriptome analysis revealed the regulation of gibberellin and the establishment of photosynthetic system promote rapid seed germination and early growth of seedling in pearl millet. Biotechnol Biofuels 14(1):94. https:\/\/doi.org\/10.1186\/s13068-021-01946-6","journal-title":"Biotechnol Biofuels"},{"issue":"24","key":"3887_CR55","doi-asserted-by":"publisher","first-page":"3595","DOI":"10.3390\/PLANTS13243595","volume":"13","author":"X Wu","year":"2024","unstructured":"Wu X, Hu X, Bao Q et al (2024) Genome-wide identification and expression analysis of nac gene family members in seashore paspalum under salt stress. Plants 13(24):3595. https:\/\/doi.org\/10.3390\/PLANTS13243595","journal-title":"Plants"},{"issue":"1","key":"3887_CR56","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1186\/s12864-024-10209-1","volume":"25","author":"F Xing","year":"2024","unstructured":"Xing F, Zhang L, Ge W et al (2024) Comparative transcriptome analysis reveals the importance of phenylpropanoid biosynthesis for the induced resistance of 84K poplar to anthracnose. BMC Genomics 25(1):306. https:\/\/doi.org\/10.1186\/s12864-024-10209-1","journal-title":"BMC Genomics"},{"key":"3887_CR57","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1093\/PLPHYS\/KIAE674","volume":"197","author":"Y Yang","year":"2025","unstructured":"Yang Y, Kong Q, Ma Z et al (2025) Phase separation of MYB73 regulates seed oil biosynthesis in arabidopsis. Plant Physiol 197:674. https:\/\/doi.org\/10.1093\/PLPHYS\/KIAE674","journal-title":"Plant Physiol"},{"key":"3887_CR58","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1089\/OMI.2011.0118","volume":"16","author":"G Yu","year":"2012","unstructured":"Yu G, Wang LG, Han Y, He QY (2012) ClusterProfiler: an r package for comparing biological themes among gene clusters. OMICS 16:284\u2013287. https:\/\/doi.org\/10.1089\/OMI.2011.0118","journal-title":"OMICS"},{"key":"3887_CR59","doi-asserted-by":"publisher","DOI":"10.1016\/J.MARENVRES.2020.105012","author":"A Zenone","year":"2020","unstructured":"Zenone A, Alagna A, D\u2019Anna G et al (2020) Biological adhesion in seagrasses: the role of substrate roughness in Posidonia oceanica (L.) Delile seedling anchorage via adhesive root hairs. Mar Environ Res. https:\/\/doi.org\/10.1016\/J.MARENVRES.2020.105012","journal-title":"Mar Environ Res"},{"key":"3887_CR60","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-023-04616-x","author":"Y Zhang","year":"2023","unstructured":"Zhang Y, Yue S, Liu M et al (2023) Combined transcriptome and proteome analysis reveal the key physiological processes in seed germination stimulated by decreased salinity in the seagrass Zostera marina L. BMC Plant Biol. https:\/\/doi.org\/10.1186\/s12870-023-04616-x","journal-title":"BMC Plant Biol"},{"key":"3887_CR61","doi-asserted-by":"publisher","DOI":"10.3389\/FPLS.2025.1497064","volume":"16","author":"H Zhao","year":"2025","unstructured":"Zhao H, Dong X, Yang D et al (2025) New insights into the salt-responsive regulation in eelgrass at transcriptional and post-transcriptional levels. Front Plant Sci 16:1497064. https:\/\/doi.org\/10.3389\/FPLS.2025.1497064","journal-title":"Front Plant Sci"},{"key":"3887_CR62","doi-asserted-by":"publisher","DOI":"10.3389\/FPLS.2023.1130292","volume":"14","author":"M Zhu","year":"2023","unstructured":"Zhu M, Zang Y, Zhang X et al (2023) Insights into the regulation of energy metabolism during the seed-to-seedling transition in marine angiosperm Zostera marina L.: Integrated metabolomic and transcriptomic analysis. Front Plant Sci 14:1130292. https:\/\/doi.org\/10.3389\/FPLS.2023.1130292","journal-title":"Front Plant Sci"}],"container-title":["Plant Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00299-026-03887-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00299-026-03887-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00299-026-03887-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T16:17:32Z","timestamp":1783613852000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00299-026-03887-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":61,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["3887"],"URL":"https:\/\/doi.org\/10.1007\/s00299-026-03887-6","relation":{},"ISSN":["0721-7714","1432-203X"],"issn-type":[{"value":"0721-7714","type":"print"},{"value":"1432-203X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]},"assertion":[{"value":"19 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 June 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"208"}}