{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:11:34Z","timestamp":1792782694682,"version":"4.5.0"},"reference-count":78,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32360743"],"award-info":[{"award-number":["32360743"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Plant Cell Rep"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1007\/s00299-026-03954-y","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:58:23Z","timestamp":1787842703000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protein persulfidation in plants: a central regulator of multiple signaling pathways"],"prefix":"10.1007","volume":"45","author":[{"given":"Di","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengjing","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinfang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7404-2145","authenticated-orcid":false,"given":"Weibiao","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"issue":"5","key":"3954_CR1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12990","volume":"76","author":"MS Aghdam","year":"2024","unstructured":"Aghdam MS, Arnao MB (2024) Phytomelatonin: from intracellular signaling to global horticulture market. J Pineal Res 76(5):e12990. https:\/\/doi.org\/10.1111\/jpi.12990","journal-title":"J Pineal Res"},{"issue":"1","key":"3954_CR2","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.tplants.2018.10.010","volume":"24","author":"MB Arnao","year":"2019","unstructured":"Arnao MB, Hern\u00e1ndez-Ruiz J (2019) Melatonin: a new plant hormone and\/or a plant master regulator? Trends Plant Sci 24(1):38\u201348. https:\/\/doi.org\/10.1016\/j.tplants.2018.10.010","journal-title":"Trends Plant Sci"},{"issue":"2","key":"3954_CR3","doi-asserted-by":"publisher","first-page":"327","DOI":"10.3390\/antiox11020327","volume":"11","author":"A Aroca","year":"2022","unstructured":"Aroca A, Gotor C (2022) Hydrogen sulfide: a key role in autophagy regulation from plants to mammalians. Antioxidants (Basel) 11(2):327. https:\/\/doi.org\/10.3390\/antiox11020327","journal-title":"Antioxidants (Basel)"},{"issue":"1","key":"3954_CR4","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1104\/pp.15.00009","volume":"168","author":"\u00c1 Aroca","year":"2015","unstructured":"Aroca \u00c1, Serna A, Gotor C, Romero LC (2015) S-sulfhydration: a cysteine posttranslational modification in plant systems. Plant Physiol 168(1):334\u2013342. https:\/\/doi.org\/10.1104\/pp.15.00009","journal-title":"Plant Physiol"},{"issue":"17","key":"3954_CR5","doi-asserted-by":"publisher","first-page":"4915","DOI":"10.1093\/jxb\/erx294","volume":"68","author":"A Aroca","year":"2017","unstructured":"Aroca A, Benito JM, Gotor C, Romero LC (2017) Persulfidation proteome reveals the regulation of protein function by hydrogen sulfide in diverse biological processes in Arabidopsis. J Exp Bot 68(17):4915\u20134927. https:\/\/doi.org\/10.1093\/jxb\/erx294","journal-title":"J Exp Bot"},{"issue":"2","key":"3954_CR6","doi-asserted-by":"publisher","first-page":"48","DOI":"10.3390\/antiox8020048","volume":"8","author":"D Benchoam","year":"2019","unstructured":"Benchoam D, Cuevasanta E, M\u00f6ller MN, Alvarez B (2019) Hydrogen sulfide and persulfides oxidation by biologically relevant oxidizing species. Antioxidants (Basel) 8(2):48. https:\/\/doi.org\/10.3390\/antiox8020048","journal-title":"Antioxidants (Basel)"},{"issue":"1","key":"3954_CR7","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22001-w","volume":"12","author":"N Bithi","year":"2021","unstructured":"Bithi N, Link C, Henderson YO, Kim S, Yang J, Li L, Wang R, Willard B, Hine C (2021) Dietary restriction transforms the mammalian protein persulfidome in a tissue-specific and cystathionine \u03b3-lyase-dependent manner. Nat Commun 12(1):1745. https:\/\/doi.org\/10.1038\/s41467-021-22001-w","journal-title":"Nat Commun"},{"issue":"2","key":"3954_CR8","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1111\/nph.18159","volume":"235","author":"C Canc\u00e9","year":"2022","unstructured":"Canc\u00e9 C, Martin-Arevalillo R, Boubekeur K, Dumas R (2022) Auxin response factors are keys to the many auxin doors. New Phytol 235(2):402\u2013419. https:\/\/doi.org\/10.1111\/nph.18159","journal-title":"New Phytol"},{"key":"3954_CR9","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2025.109644","volume":"221","author":"R Carrillo","year":"2025","unstructured":"Carrillo R, Moreno I, Romero LC, Aroca A, Gotor C (2025) Hydrogen sulfide-induced barley resilience to drought and salinity through protein persulfidation. Plant Physiol Biochem 221:109644. https:\/\/doi.org\/10.1016\/j.plaphy.2025.109644","journal-title":"Plant Physiol Biochem"},{"issue":"5","key":"3954_CR10","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1016\/j.molp.2020.01.004","volume":"13","author":"S Chen","year":"2020","unstructured":"Chen S, Jia H, Wang X, Shi C, Wang X, Ma P, Wang J, Ren M, Li J (2020) Hydrogen sulfide positively regulates abscisic acid signaling through persulfidation of SnRK2.6 in guard cells. Mol Plant 13(5):732\u2013744. https:\/\/doi.org\/10.1016\/j.molp.2020.01.004","journal-title":"Mol Plant"},{"issue":"1","key":"3954_CR11","volume":"2","author":"FK Choudhury","year":"2025","unstructured":"Choudhury FK, DeNicola GM (2025) Best practices for cysteine analysis. Ferroptosis Oxidative Stress 2(1):202508","journal-title":"Ferroptosis Oxidative Stress"},{"key":"3954_CR12","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.707797","volume":"9","author":"CE Cooper","year":"2021","unstructured":"Cooper CE, Bird M, Sheng X, Choi JW, Silkstone GG, Simons M, Bettati S (2021) Stability of maleimide-PEG and mono-sulfone-PEG conjugation to a novel engineered cysteine in the human hemoglobin alpha subunit. Front Chem 9:707797. https:\/\/doi.org\/10.3389\/fchem.2021.707797","journal-title":"Front Chem"},{"key":"3954_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2020.126688","volume":"245","author":"F Diao","year":"2021","unstructured":"Diao F, Dang Z, Xu J, Ding S, Hao B, Zhang Z, Zhang J, Wang L, Guo W (2021) Effect of arbuscular mycorrhizal symbiosis on ion homeostasis and salt tolerance-related gene expression in halophyte Suaeda salsa under salt treatments. Microbiol Res 245:126688. https:\/\/doi.org\/10.1016\/j.micres.2020.126688","journal-title":"Microbiol Res"},{"key":"3954_CR14","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1007\/978-1-4939-9528-85","volume":"2007","author":"\u00c9 D\u00f3ka","year":"2019","unstructured":"D\u00f3ka \u00c9, Arn\u00e9r ES, Schmidt EE, Nagy P (2019) ProPerDP: a protein persulfide detection protocol. In Vascular Effects Hydrogen Sulfide Methods Protocols 2007:51\u201377. https:\/\/doi.org\/10.1007\/978-1-4939-9528-85","journal-title":"In Vascular Effects Hydrogen Sulfide Methods Protocols"},{"issue":"7","key":"3954_CR15","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1016\/j.molp.2023.06.008","volume":"16","author":"SM Dubey","year":"2023","unstructured":"Dubey SM, Han S, Stutzman N, Prigge MJ, Medveck\u00e1 E, Platre MP, Busch W, Fendrych M, Estelle M (2023) The AFB1 auxin receptor controls the cytoplasmic auxin response pathway in Arabidopsis thaliana. Mol Plant 16(7):1120\u20131130. https:\/\/doi.org\/10.1016\/j.molp.2023.06.008","journal-title":"Mol Plant"},{"issue":"4","key":"3954_CR16","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1016\/j.tplants.2018.01.003","volume":"23","author":"M Dubois","year":"2018","unstructured":"Dubois M, Van den Broeck L, Inz\u00e9 D (2018) The pivotal role of ethylene in plant growth. Trends Plant Sci 23(4):311\u2013323. https:\/\/doi.org\/10.1016\/j.tplants.2018.01.003","journal-title":"Trends Plant Sci"},{"issue":"3","key":"3954_CR17","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1021\/acs.chemrev.7b00205","volume":"118","author":"MR Filipovic","year":"2018","unstructured":"Filipovic MR, Zivanovic J, Alvarez B, Banerjee R (2018) Chemical biology of H2S signaling through persulfidation. Chem Rev 118(3):1253\u20131337. https:\/\/doi.org\/10.1021\/acs.chemrev.7b00205","journal-title":"Chem Rev"},{"key":"3954_CR18","doi-asserted-by":"crossref","unstructured":"Filipovic, M. R. 2015. Persulfidation (S-sulfhydration) and H2S. Chemistry, Biochemistry and Pharmacology of Hydrogen Sulfide: 29\u201359.","DOI":"10.1007\/978-3-319-18144-8_2"},{"key":"3954_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2024.113159","volume":"331","author":"A Ghorbani","year":"2024","unstructured":"Ghorbani A, Pehlivan N, Zargar M, Chen M (2024) Synergistic role of melatonin and hydrogen sulfide in modulating secondary metabolites and metal uptake\/sequestration in arsenic-stressed tomato plants. Sci Hortic 331:113159. https:\/\/doi.org\/10.1016\/j.scienta.2024.113159","journal-title":"Sci Hortic"},{"issue":"16","key":"3954_CR20","doi-asserted-by":"publisher","first-page":"4251","DOI":"10.1093\/jxb\/erz225","volume":"70","author":"C Gotor","year":"2019","unstructured":"Gotor C, Garc\u00eda I, Aroca \u00c1, Laureano-Mar\u00edn AM, Arenas-Alfonseca L, Jurado-Flores A, Moren I, Romero LC (2019) Signaling by hydrogen sulfide and cyanide through post-translational modification. J Exp Bot 70(16):4251\u20134265. https:\/\/doi.org\/10.1093\/jxb\/erz225","journal-title":"J Exp Bot"},{"key":"3954_CR21","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2023.115444","volume":"209","author":"B He","year":"2023","unstructured":"He B, Zhang Z, Huang Z, Duan X, Wang Y, Cao J, Li L, He K, Nice EC, He W, Gao W, Shen Z (2023) Protein persulfidation: rewiring the hydrogen sulfide signaling in cell stress response. Biochem Pharmacol 209:115444. https:\/\/doi.org\/10.1016\/j.bcp.2023.115444","journal-title":"Biochem Pharmacol"},{"issue":"86","key":"3954_CR22","doi-asserted-by":"publisher","DOI":"10.1126\/stke.2001.86.pl1","volume":"2001","author":"SR Jaffrey","year":"2001","unstructured":"Jaffrey SR, Snyder SH (2001) The biotin switch method for the detection of S-nitrosylated proteins. Sci STKE 2001(86):pl1. https:\/\/doi.org\/10.1126\/stke.2001.86.pl1","journal-title":"Sci STKE"},{"issue":"3","key":"3954_CR23","doi-asserted-by":"publisher","first-page":"2445","DOI":"10.1111\/pce.15309","volume":"48","author":"S Jaiswal","year":"2025","unstructured":"Jaiswal S, Singh SP, Singh S, Gupta R, Tripathi DK, Corpas FJ, Singh VP (2025) Hydrogen sulphide: a key player in plant development and stress resilience. Plant Cell Environ 48(3):2445\u20132459. https:\/\/doi.org\/10.1111\/pce.15309","journal-title":"Plant Cell Environ"},{"key":"3954_CR24","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01517","volume":"9","author":"H Jia","year":"2018","unstructured":"Jia H, Chen S, Liu D, Liesche J, Shi C, Wang J, Ren M, Wang X, Yang J, Shi W, Li J (2018) Ethylene-induced hydrogen sulfide negatively regulates ethylene biosynthesis by persulfidation of ACO in tomato under osmotic stress. Front Plant Sci 9:1517. https:\/\/doi.org\/10.3389\/fpls.2018.01517","journal-title":"Front Plant Sci"},{"key":"3954_CR25","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00678","author":"J Jiang","year":"2019","unstructured":"Jiang J, Tian Y, Li L, Yu M, Hou R, Ren X (2019) H2S alleviates salinity stress in cucumber by maintaining the Na+\/K+ balance and regulating H2S metabolism and oxidative stress response. Front Plant Sci. https:\/\/doi.org\/10.3389\/fpls.2019.00678","journal-title":"Front Plant Sci"},{"key":"3954_CR26","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2023.108231","volume":"206","author":"MN Khan","year":"2024","unstructured":"Khan MN, Siddiqui MH, AlSolami MA, Siddiqui ZH (2024) Melatonin-regulated heat shock proteins and mitochondrial ATP synthase induce drought tolerance through sustaining ROS homeostasis in H2S-dependent manner. Plant Physiol Biochem 206:108231. https:\/\/doi.org\/10.1016\/j.plaphy.2023.108231","journal-title":"Plant Physiol Biochem"},{"key":"3954_CR27","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.jplph.2018.05.010","volume":"228","author":"N Kou","year":"2018","unstructured":"Kou N, Xiang Z, Cui W, Li L, Shen W (2018) Hydrogen sulfide acts downstream of methane to induce cucumber adventitious root development. J Plant Physiol 228:113\u2013120. https:\/\/doi.org\/10.1016\/j.jplph.2018.05.010","journal-title":"J Plant Physiol"},{"issue":"203","key":"3954_CR28","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2002329","volume":"4","author":"N Krishnan","year":"2011","unstructured":"Krishnan N, Fu C, Pappin DJ, Tonks NK (2011) H2S-induced sulfhydration of the phosphatase PTP1B and its role in the endoplasmic reticulum stress response. Sci Signal 4(203):ra86. https:\/\/doi.org\/10.1126\/scisignal.2002329","journal-title":"Sci Signal"},{"key":"3954_CR29","doi-asserted-by":"crossref","unstructured":"Laureano-Mar\u00edn, A. M., \u00c1. Aroca, M. E. P\u00e9rez-P\u00e9rez, I. Yruela, A. Jurado-Flores, I. Moreno, J. L. Crespo, L. C. Romero and C. Gotor. 2020. Abscisic acid-triggered persulfidation of the cys protease ATG4 mediates regulation of autophagy by sulfide. The Plant Cell, 32(12): 3902\u20133920. https:\/\/www.jstor.org\/stable\/27014372","DOI":"10.1105\/tpc.20.00766"},{"issue":"17","key":"3954_CR30","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25179679","volume":"25","author":"M Li","year":"2024","unstructured":"Li M, Xiao L, Sun K, Qiu T, Lai S, Chen G, Geng L, Huang S, Xie Y (2024) Insights from structure-based simulations into the persulfidation of uridine diphosphate-glycosyltransferase 71c5 facilitating the reversible inactivation of abscisic acid. Int J Mol Sci 25(17):9679. https:\/\/doi.org\/10.3390\/ijms25179679","journal-title":"Int J Mol Sci"},{"issue":"4","key":"3954_CR31","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1042\/ebc20230095","volume":"68","author":"H Liu","year":"2024","unstructured":"Liu H, Negoita F, Brook M, Sakamoto K, Morton NM (2024) Quantification of persulfidation on specific proteins: are we nearly there yet? Essays Biochem 68(4):467\u2013478. https:\/\/doi.org\/10.1042\/ebc20230095","journal-title":"Essays Biochem"},{"issue":"5","key":"3954_CR32","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1111\/jipb.13851","volume":"67","author":"H Liu","year":"2025","unstructured":"Liu H, Liang X, Liu R, Liu C, Luo S, Zhang Z, Liu Z, Xue S (2025) Hydrogen sulfide inhibits Arabidopsis inward potassium channels via protein persulfidation. J Integr Plant Biol 67(5):1217\u20131219. https:\/\/doi.org\/10.1111\/jipb.13851","journal-title":"J Integr Plant Biol"},{"issue":"1","key":"3954_CR33","doi-asserted-by":"publisher","DOI":"10.1038\/srep29808","volume":"6","author":"S Longen","year":"2016","unstructured":"Longen S, Richter F, K\u00f6hler Y, Wittig I, Beck KF, Pfeilschifter J (2016) Quantitative persulfide site identification (qPerS-SID) reveals protein targets of H2S releasing donors in mammalian cells. Sci Rep 6(1):29808. https:\/\/doi.org\/10.1038\/srep29808","journal-title":"Sci Rep"},{"issue":"13\u201315","key":"3954_CR34","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1089\/ars.2022.0064","volume":"39","author":"S Luo","year":"2023","unstructured":"Luo S, Kong C, Ye D, Liu X, Wang Y, Meng G, Ji Y (2023) Protein persulfidation: recent progress and future directions. Antioxid Redox Signal 39(13\u201315):829\u2013852. https:\/\/doi.org\/10.1089\/ars.2022.0064","journal-title":"Antioxid Redox Signal"},{"issue":"3","key":"3954_CR35","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1111\/jipb.13030","volume":"63","author":"Y Ma","year":"2021","unstructured":"Ma Y, Cao J, Chen Q, He J, Liu Z, Wang J, Li X, Yang Y (2021b) Abscisic acid receptors maintain abscisic acid homeostasis by modulating UGT71C5 glycosylation activity. J Integr Plant Biol 63(3):543\u2013552. https:\/\/doi.org\/10.1111\/jipb.13030","journal-title":"J Integr Plant Biol"},{"issue":"8","key":"3954_CR36","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1007\/s00299-023-03029-2","volume":"42","author":"Y Ma","year":"2023","unstructured":"Ma Y, Li F, Yi Y, Wang X, Li T, Wang X, Sun H, Li L, Ren M, Han S, Zhang L, Chen Y, Tang H, Jia H, Li J (2023) Hydrogen sulfide improves salt tolerance through persulfidation of PMA1 in Arabidopsis. Plant Cell Rep 42(8):1265\u20131277. https:\/\/doi.org\/10.1007\/s00299-023-03029-2","journal-title":"Plant Cell Rep"},{"key":"3954_CR37","doi-asserted-by":"publisher","unstructured":"Ma, X., L. Zhang, Z. Pei, L. Zhang, Z. Liu, D. Liu, X. Hao, Z. Jin, and Y. Pei. 2021a. Hydrogen sulfide promotes flowering in heading Chinese cabbage by S-sulfhydration of BraFLCs. Horticulture Research, 8. https:\/\/doi.org\/10.1038\/s41438-020-00453-3","DOI":"10.1038\/s41438-020-00453-3"},{"issue":"17","key":"3954_CR38","doi-asserted-by":"publisher","first-page":"4215","DOI":"10.1093\/jxb\/ery201","volume":"69","author":"P Manishankar","year":"2018","unstructured":"Manishankar P, Wang N, K\u00f6ster P, Alatar AA, Kudla J (2018) Calcium signaling during salt stress and in the regulation of ion homeostasis. J Exp Bot 69(17):4215\u20134226. https:\/\/doi.org\/10.1093\/jxb\/ery201","journal-title":"J Exp Bot"},{"issue":"1","key":"3954_CR39","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-017-1110-7","volume":"17","author":"Y Mei","year":"2017","unstructured":"Mei Y, Chen H, Shen W, Shen W, Huang L (2017) Hydrogen peroxide is involved in hydrogen sulfide-induced lateral root formation in tomato seedlings. BMC Plant Biol 17(1):162. https:\/\/doi.org\/10.1186\/s12870-017-1110-7","journal-title":"BMC Plant Biol"},{"issue":"1","key":"3954_CR40","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-66795-5","volume":"17","author":"JL Miljkovic","year":"2025","unstructured":"Miljkovic JL, Burger N, Yu CS, Harkiss AH, Warrington S, Caldwell ST, James AM (2025) Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H2S in biological samples. Nat Commun 17(1):85. https:\/\/doi.org\/10.1038\/s41467-025-66795-5","journal-title":"Nat Commun"},{"key":"3954_CR41","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.niox.2018.11.003","volume":"82","author":"S Mukherjee","year":"2019","unstructured":"Mukherjee S (2019) Recent advancements in the mechanism of nitric oxide signaling associated with hydrogen sulfide and melatonin crosstalk during ethylene-induced fruit ripening in plants. Nitric Oxide 82:25\u201334. https:\/\/doi.org\/10.1016\/j.niox.2018.11.003","journal-title":"Nitric Oxide"},{"issue":"96","key":"3954_CR42","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000464","volume":"2","author":"AK Mustafa","year":"2009","unstructured":"Mustafa AK, Gadalla MM, Sen N, Kim S, Mu W, Gazi SK, Snyder SH (2009) H2S signals through protein S-sulfhydration. Sci Signal 2(96):ra72. https:\/\/doi.org\/10.1126\/scisignal.2000464","journal-title":"Sci Signal"},{"issue":"6","key":"3954_CR43","doi-asserted-by":"publisher","first-page":"1110","DOI":"10.1021\/cb4001052","volume":"8","author":"J Pan","year":"2013","unstructured":"Pan J, Carroll KS (2013) Persulfide reactivity in the detection of protein S-sulfhydration. ACS Chem Biol 8(6):1110\u20131116. https:\/\/doi.org\/10.1021\/cb4001052","journal-title":"ACS Chem Biol"},{"issue":"11","key":"3954_CR44","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/j.tibs.2015.08.007","volume":"40","author":"BD Paul","year":"2015","unstructured":"Paul BD, Snyder SH (2015) H2S: a novel gasotransmitter that signals by sulfhydration. Trends Biochem Sci 40(11):687\u2013700. https:\/\/doi.org\/10.1016\/j.tibs.2015.08.007","journal-title":"Trends Biochem Sci"},{"issue":"23","key":"3954_CR45","doi-asserted-by":"publisher","first-page":"6150","DOI":"10.1111\/febs.12535","volume":"280","author":"JA Reisz","year":"2013","unstructured":"Reisz JA, Bechtold E, King SB, Poole LB, Furdui CM (2013) Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids. FEBS J 280(23):6150\u20136161. https:\/\/doi.org\/10.1111\/febs.12535","journal-title":"FEBS J"},{"issue":"2","key":"3954_CR46","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1093\/jxb\/erx237","volume":"69","author":"M Roosjen","year":"2018","unstructured":"Roosjen M, Paque S, Weijers D (2018) Auxin response factors: output control in auxin biology. J Exp Bot 69(2):179\u2013188. https:\/\/doi.org\/10.1093\/jxb\/erx237","journal-title":"J Exp Bot"},{"issue":"30","key":"3954_CR47","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202203684","volume":"134","author":"D Schilling","year":"2022","unstructured":"Schilling D, Barayeu U, Steimbach RR, Talwar D, Miller AK, Dick TP (2022) Commonly used alkylating agents limit persulfide detection by converting protein persulfides into thioethers. Angew Chem Int Ed Engl 134(30):e202203684. https:\/\/doi.org\/10.1002\/anie.202203684","journal-title":"Angew Chem Int Ed Engl"},{"issue":"1","key":"3954_CR48","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.molcel.2011.10.021","volume":"45","author":"N Sen","year":"2012","unstructured":"Sen N, Paul BD, Gadalla MM, Mustafa AK, Sen T, Xu R, Snyder SH (2012) Hydrogen sulfide-linked sulfhydration of NF-\u03baB mediates its antiapoptotic actions. Mol Cell 45(1):13\u201324. https:\/\/doi.org\/10.1016\/j.molcel.2011.10.021","journal-title":"Mol Cell"},{"key":"3954_CR49","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1007\/978-1-4939-7695-9_16","volume-title":"Nitric oxide: methods and protocols","author":"A Shekariesfahlan","year":"2018","unstructured":"Shekariesfahlan A, Lindermayr C (2018) Detection of S-nitrosated nuclear proteins in pathogen-treated Arabidopsis cell cultures using biotin switch technique. In: Mengel A, Lindermayr C (eds) Nitric oxide: methods and protocols. Springer New York, New York, NY, pp 205\u2013221. https:\/\/doi.org\/10.1007\/978-1-4939-7695-9_16"},{"issue":"4","key":"3954_CR50","doi-asserted-by":"publisher","first-page":"1000","DOI":"10.1105\/tpc.19.00826","volume":"32","author":"J Shen","year":"2020","unstructured":"Shen J, Zhang J, Zhou M, Zhou H, Cui B, Gotor C, Romero L, Fu L, Yang J, Foyer C, Pan Q, Shen W, Xie Y (2020) Persulfidation-based modification of cysteine desulfhydrase and the NADPH oxidase RBOHD controls guard cell abscisic acid signaling. Plant Cell 32(4):1000\u20131017. https:\/\/doi.org\/10.1105\/tpc.19.00826","journal-title":"Plant Cell"},{"key":"3954_CR51","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2023.108306","volume":"206","author":"Y Shi","year":"2024","unstructured":"Shi Y, Li Y, Liu T, Guo C, Liang W, Ma F, Li C (2024) \u03b3-Aminobutyric acid enhances salt tolerance by sustaining ion homeostasis in apples. Plant Physiol Biochem 206:108306. https:\/\/doi.org\/10.1016\/j.plaphy.2023.108306","journal-title":"Plant Physiol Biochem"},{"issue":"8","key":"3954_CR52","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1007\/s00299-021-02731-3","volume":"40","author":"MH Siddiqui","year":"2021","unstructured":"Siddiqui MH, Khan MN, Mukherjee S, Alamri S, Basahi RA, Al-Amri AA, Alsubaie QD, Al-Munqedhi BMA, Ali HM, Almohisen IAA (2021) Hydrogen sulfide (H2S) and potassium (K+) synergistically induce drought stress tolerance through regulation of H+-ATPase activity, sugar metabolism, and antioxidative defense in tomato seedlings. Plant Cell Rep 40(8):1543\u20131564. https:\/\/doi.org\/10.1007\/s00299-021-02731-3","journal-title":"Plant Cell Rep"},{"key":"3954_CR53","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.plaphy.2021.07.023","volume":"167","author":"Y Sun","year":"2021","unstructured":"Sun Y, Ma C, Kang X, Zhang L, Wang J, Zheng S, Zhang T (2021) Hydrogen sulfide and nitric oxide are involved in melatonin-induced salt tolerance in cucumber. Plant Physiol Biochem 167:101\u2013112. https:\/\/doi.org\/10.1016\/j.plaphy.2021.07.023","journal-title":"Plant Physiol Biochem"},{"issue":"1","key":"3954_CR54","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1093\/plphys\/kiad070","volume":"192","author":"C Sun","year":"2023","unstructured":"Sun C, Yao G, Li L, Li T, Zhao Y, Hu K, Zhang C, Zhang H (2023) E3 ligase BRG3 persulfidation delays tomato ripening by reducing ubiquitination of the repressor WRKY71. Plant Physiol 192(1):616\u2013632. https:\/\/doi.org\/10.1093\/plphys\/kiad070","journal-title":"Plant Physiol"},{"issue":"4","key":"3954_CR55","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.70000","volume":"121","author":"Y Sun","year":"2025","unstructured":"Sun Y, Zhang M, Geng M, Geng Z, Lu Z, Liu N, Liu Z, Zeng D, Yao G, Hu K (2025) Hydrogen sulfide interferes with ethylene biosynthesis and signaling pathway in tomato by the mediation of SlERF. D2 persulfidation. Plant J 121(4):e70000. https:\/\/doi.org\/10.1111\/tpj.70000","journal-title":"Plant J"},{"issue":"1\u20133","key":"3954_CR56","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1089\/ars.2023.0352","volume":"39","author":"T Vignane","year":"2023","unstructured":"Vignane T, Filipovic MR (2023) Emerging chemical biology of protein persulfidation. Antioxid Redox Signal 39(1\u20133):19\u201339. https:\/\/doi.org\/10.1089\/ars.2023.0352","journal-title":"Antioxid Redox Signal"},{"issue":"42","key":"3954_CR57","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2504783122","volume":"122","author":"GM Visani","year":"2025","unstructured":"Visani GM, Pun MN, Minervina AA, Bradley P, Thomas PG, Nourmohammad A (2025) T cell receptor specificity landscape revealed through de novo peptide design. Proc Natl Acad Sci U S A 122(42):e2504783122. https:\/\/doi.org\/10.1073\/pnas.2504783122","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"3954_CR58","doi-asserted-by":"publisher","first-page":"1631","DOI":"10.3390\/antiox10101631","volume":"10","author":"P Wang","year":"2021","unstructured":"Wang P, Fang H, Gao R, Liao W (2021a) Protein persulfidation in plants: function and mechanism. MDPI Antioxidants 10(10):1631. https:\/\/doi.org\/10.3390\/antiox10101631","journal-title":"MDPI Antioxidants"},{"issue":"2","key":"3954_CR59","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1007\/s00299-021-02813-2","volume":"41","author":"Z Wang","year":"2021","unstructured":"Wang Z, Mu Y, Hao X, Yang J, Zhang D, Jin Z, Pei Y (2021b) H2S aids osmotic stress resistance by S-sulfhydration of melatonin production-related enzymes in Arabidopsis thaliana. Plant Cell Rep 41(2):365\u2013376. https:\/\/doi.org\/10.1007\/s00299-021-02813-2","journal-title":"Plant Cell Rep"},{"issue":"2","key":"3954_CR60","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1111\/nph.19188","volume":"240","author":"X Wang","year":"2023","unstructured":"Wang X, Shi C, Hu Y, Ma Y, Yi Y, Jia H, Li F, Sun H, Li T, Wang X, Li T, Li J (2023) Persulfidation maintains cytosolic G6PDs activity through changing tetrameric structure and competing cysteine sulfur oxidation under salt stress in Arabidopsis and tomato. New Phytol 240(2):626\u2013643. https:\/\/doi.org\/10.1111\/nph.19188","journal-title":"New Phytol"},{"issue":"3","key":"3954_CR61","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1016\/j.hpj.2023.03.011","volume":"10","author":"X Wang","year":"2024","unstructured":"Wang X, Cao M, Li H, Liu Y, Fan S, Zhang N, Guo Y (2024a) Strategies and prospects for melatonin to alleviate abiotic stress in horticultural plants. Hortic Plant J 10(3):601\u2013614. https:\/\/doi.org\/10.1016\/j.hpj.2023.03.011","journal-title":"Hortic Plant J"},{"issue":"4","key":"3954_CR62","doi-asserted-by":"publisher","first-page":"1377","DOI":"10.1111\/nph.20122","volume":"244","author":"X Wang","year":"2024","unstructured":"Wang X, Liu C, Li T, Zhou F, Sun H, Li F, Ma Y, Jia H, Zhang X, Shi W, Gong C, Li J (2024b) Hydrogen sulfide antagonizes cytokinin to change root system architecture through persulfidation of CKX2 in Arabidopsis. New Phytol 244(4):1377\u20131390. https:\/\/doi.org\/10.1111\/nph.20122","journal-title":"New Phytol"},{"issue":"9","key":"3954_CR63","doi-asserted-by":"publisher","first-page":"1812","DOI":"10.1093\/treephys\/tpac042","volume":"42","author":"MY Wei","year":"2022","unstructured":"Wei MY, Li H, Zhang L, Guo Z, Liu J, Ding Q, Zhong Y, Li J, Ma D, Zheng H, Ball M (2022) Exogenous hydrogen sulfide mediates Na+ and K+ fluxes of salt gland in salt-secreting mangrove plant Avicennia marina. Tree Physiol 42(9):1812\u20131826. https:\/\/doi.org\/10.1093\/treephys\/tpac042","journal-title":"Tree Physiol"},{"issue":"6","key":"3954_CR64","doi-asserted-by":"publisher","first-page":"1369","DOI":"10.1111\/tpj.16198","volume":"114","author":"ZX Xiang","year":"2023","unstructured":"Xiang ZX, Li W, Lu YT, Yuan TT (2023) Hydrogen sulfide alleviates osmotic stress-induced root growth inhibition by promoting auxin homeostasis. Plant J 114(6):1369\u20131384. https:\/\/doi.org\/10.1111\/tpj.16198","journal-title":"Plant J"},{"issue":"49","key":"3954_CR65","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202513587","volume":"137","author":"S Xiao","year":"2025","unstructured":"Xiao S, Chang Y, Jia P, Li X, Li X, Li X, Yuan H, Li J, Zhang Z, Liu M (2025) Site-specific recognition of inosine-modified RNA by deoxyribozyme with distant cleavage site. Angew Chem 137(49):e202513587. https:\/\/doi.org\/10.1002\/anie.202513587","journal-title":"Angew Chem"},{"key":"3954_CR66","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2023.105354","volume":"211","author":"L Yang","year":"2023","unstructured":"Yang L, Yang J, Hou C, Shi P, Zhang Y, Gu X, Liu W (2023) Hydrogen sulfide alleviates salt stress through auxin signaling in Arabidopsis. Environ Exp Bot 211:105354. https:\/\/doi.org\/10.1016\/j.envexpbot.2023.105354","journal-title":"Environ Exp Bot"},{"key":"3954_CR67","unstructured":"Yapa Abeywardana M (2022) Chemical Proteomic Identification And Functional Studies Of Cardiac Protein Glutathionylation"},{"issue":"2","key":"3954_CR68","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1002\/anie.201305876","volume":"53","author":"D Zhang","year":"2014","unstructured":"Zhang D, Macinkovic I, Devarie-Baez NO, Pan J, Park CM, Carroll KS, Xian M (2014) Detection of protein S-sulfhydration by a tag-switch technique. Angew Chem Int Ed Engl 53(2):575\u2013581. https:\/\/doi.org\/10.1002\/anie.201305876","journal-title":"Angew Chem Int Ed Engl"},{"issue":"1","key":"3954_CR69","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01046-2","volume":"7","author":"P Zhang","year":"2017","unstructured":"Zhang P, Luo Q, Wang R, Xu J (2017) Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway. Sci Rep 7(1):868. https:\/\/doi.org\/10.1038\/s41598-017-01046-2","journal-title":"Sci Rep"},{"issue":"3","key":"3954_CR70","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1111\/pce.13685","volume":"43","author":"J Zhang","year":"2019","unstructured":"Zhang J, Zhou M, Ge Z, Shen J, Zhou C, Gotor C, Romero LC, Duan X, Liu X, Wu D, Yin X, Xie Y (2019) Abscisic acid-triggered guard cell L-cysteine desulfhydrase function and in situ hydrogen sulfide production contributes to heme oxygenase-modulated stomatal closure. Plant Cell Environ 43(3):624\u2013636. https:\/\/doi.org\/10.1111\/pce.13685","journal-title":"Plant Cell Environ"},{"issue":"1","key":"3954_CR71","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1093\/plphys\/kiae271","volume":"196","author":"M Zhang","year":"2024","unstructured":"Zhang M, Hu K, Ma L, Geng M, Zhang C, Yao G, Zhang H (2024) Persulfidation and phosphorylation of transcription factor SlWRKY6 differentially regulate tomato fruit ripening. Plant Physiol 196(1):210\u2013227. https:\/\/doi.org\/10.1093\/plphys\/kiae271","journal-title":"Plant Physiol"},{"issue":"6","key":"3954_CR72","doi-asserted-by":"publisher","first-page":"1433","DOI":"10.1111\/jipb.13886","volume":"67","author":"J Zhang","year":"2025","unstructured":"Zhang J, Li H, Xie Y (2025) A paradigm shift in stomatal regulation: H2S as a persulfidation-driven modulator of potassium channels. J Integr Plant Biol 67(6):1433\u20131434. https:\/\/doi.org\/10.1111\/jipb.13886","journal-title":"J Integr Plant Biol"},{"key":"3954_CR73","unstructured":"Zhang N (2023) Analytical approaches for the measurement of sulfide in biological and pharmaceutical fields"},{"key":"3954_CR74","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01366","volume":"9","author":"N Zhao","year":"2018","unstructured":"Zhao N, Zhu H, Zhang H, Sun J, Zhou J, Deng C, Zhang Y, Zhao R, Zhou X, Lu C, Lin S, Chen S (2018) Hydrogen sulfide mediates K+ and Na+ homeostasis in the roots of salt-resistant and salt-sensitive poplar species subjected to NaCl stress. Front Plant Sci 9:1366. https:\/\/doi.org\/10.3389\/fpls.2018.01366","journal-title":"Front Plant Sci"},{"key":"3954_CR75","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/j.plaphy.2020.08.006","volume":"155","author":"D Zhao","year":"2020","unstructured":"Zhao D, Zhang J, Zhou M, Zhou H, Gotor C, Romero LC, Shen J, Yuan X, Xie Y (2020) Current approaches for detection of hydrogen sulfide and persulfidation in biological systems. Plant Physiol Biochem 155:367\u2013373. https:\/\/doi.org\/10.1016\/j.plaphy.2020.08.006","journal-title":"Plant Physiol Biochem"},{"issue":"13","key":"3954_CR76","doi-asserted-by":"publisher","first-page":"3738","DOI":"10.1093\/jxb\/eraf100","volume":"76","author":"M Zhou","year":"2025","unstructured":"Zhou M, Xie Y, Van Breusegem F, Huang J (2025) Hydrogen sulfide and protein persulfidation in plant stress signaling. J Exp Bot 76(13):3738\u20133757. https:\/\/doi.org\/10.1093\/jxb\/eraf100","journal-title":"J Exp Bot"},{"issue":"6","key":"3954_CR77","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.1016\/j.cmet.2019.10.007","volume":"30","author":"J Zivanovic","year":"2019","unstructured":"Zivanovic J, Kouroussis E, Kohl JB, Adhikari B, Bursac B, Schott-Roux S, Petrovic D, Miljkovic JL, Thomas-Lopez D, Jung Y, Miler M, Mitchell S, Milosevic V, Gomes JE, Benhar M, Gonzalez-Zorn B, Ivanovic-Burmazovic I, Torregrossa R, Mitchell JR, Filipovic MR (2019) Selective persulfide detection reveals evolutionarily conserved antiaging effects of S-sulfhydration. Cell Metab 30(6):1152\u20131170. https:\/\/doi.org\/10.1016\/j.cmet.2019.10.007","journal-title":"Cell Metab"},{"key":"3954_CR78","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2023.107900","volume":"201","author":"D Zuo","year":"2023","unstructured":"Zuo D, Hu M, Zhou W, Lei F, Zhao J, Gu L (2023) EcAGL enhances cadmium tolerance in transgenic Arabidopsis thaliana through inhibits cadmium transport and ethylene synthesis pathway. Plant Physiol Biochem 201:107900. https:\/\/doi.org\/10.1016\/j.plaphy.2023.107900","journal-title":"Plant Physiol Biochem"}],"container-title":["Plant Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00299-026-03954-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00299-026-03954-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00299-026-03954-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T09:05:14Z","timestamp":1789635914000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00299-026-03954-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,27]]},"references-count":78,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["3954"],"URL":"https:\/\/doi.org\/10.1007\/s00299-026-03954-y","relation":{},"ISSN":["0721-7714","1432-203X"],"issn-type":[{"value":"0721-7714","type":"print"},{"value":"1432-203X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,27]]},"assertion":[{"value":"30 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 August 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"No competing interests have been identified. The authors declare no relevant financial or nonfinancial competing interests.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"No ethical issues.","order":2,"name":"Ethics","label":"Ethical approval and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All co-authors consent to the publication of the data.","order":3,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"269"}}