{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T22:25:20Z","timestamp":1793139920691,"version":"4.5.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Istanbul University"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Urol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS) is a prevalent urological condition in young men, significantly affecting quality of life due to persistent discomfort and neuropsychological symptoms. Despite its high prevalence, the etiology of CP\/CPPS remains poorly understood. This study investigated urinary microbiota differences between CP\/CPPS patients and healthy controls to identify microbial contributors, antibiotic resistance genes (ARGs), and virulence factors of dominant bacteria, as well as to explore potential therapeutic targets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Urine samples were collected from 58 CP\/CPPS patients and 25 controls. Symptom severity was assessed by a specialist urologist using the NIH Chronic Prostatitis Symptom Index and UPOINT classification. Bacterial-specific 16\u00a0S rRNA sequencing was performed using nanopore technology, with bioinformatics analyses conducted via ONT guppy 5.0.11, NCBI and SLV 16\u00a0S bacterial taxonomic databases, UPGMA hierarchical clustering, and the Bacterial and Viral Bioinformatics Resource Center (BV-BRC). Pairwise comparisons were analyzed using the Mann-Whitney U test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Distinct microbial diversity patterns were observed between patients and controls.\n                      <jats:italic>Bacillus<\/jats:italic>\n                      species were significantly enriched in CP\/CPPS patients, while\n                      <jats:italic>Enterococcus<\/jats:italic>\n                      species predominated in controls. Younger patients exhibited unique microbiome profiles compared to older groups. Bioinformatics analyses identified ARGs and virulence factors associated with\n                      <jats:italic>Bacillus<\/jats:italic>\n                      species, implicating them in localized inflammation. Antibiotics like pleuromutilin or vancomycin were identified as potential therapeutic options, though experimental validation was beyond the study\u2019s scope.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings highlight microbial imbalances and provide a foundation for microbiome-targeted therapeutic strategies for CP\/CPPS management in the future. Additionally, the identification of bacterial virulence factors and ARG provides insights into the potential mechanisms driving persistent symptoms. Future research with larger cohorts and experimental validation of the suggested therapeutic options may contribute to more effective treatment for CP\/CPPS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00345-025-05514-7","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T11:59:57Z","timestamp":1743249597000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Bioinformatic strategies in metagenomics of chronic prostatitis"],"prefix":"10.1007","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9767-3206","authenticated-orcid":false,"given":"Elmira","family":"Davasaz Tabrizi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5461-2151","authenticated-orcid":false,"given":"Mushteba","family":"Sevil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1676-5919","authenticated-orcid":false,"given":"Ercan","family":"Arican","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"issue":"3","key":"5514_CR1","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1001\/jama.282.3.236","volume":"282","author":"JN Krieger","year":"1999","unstructured":"Krieger JN, Nyberg L Jr, Nickel JC (1999) NIH consensus definition and classification of prostatitis. JAMA 282(3):236\u2013237. https:\/\/doi.org\/10.1001\/jama.282.3.236","journal-title":"JAMA"},{"issue":"11\u201312","key":"5514_CR2","doi-asserted-by":"publisher","first-page":"403","DOI":"10.5489\/cuaj.2179","volume":"8","author":"A Krsmanovic","year":"2014","unstructured":"Krsmanovic A, Tripp DA, Nickel JC, Shoskes DA, Pontari M, Litwin MS, McNaughton-Collins MF (2014) Psychosocial mechanisms of the pain and quality of life relationship for chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS). Can Urol Association J = J de l\u2019Association des urologues du Can 8(11\u201312):403\u2013408. https:\/\/doi.org\/10.5489\/cuaj.2179","journal-title":"Can Urol Association J = J de l\u2019Association des urologues du Can"},{"issue":"4","key":"5514_CR3","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1007\/s00345-013-1075-6","volume":"31","author":"DA Shoskes","year":"2013","unstructured":"Shoskes DA, Nickel JC (2013) Classification and treatment of men with chronic prostatitis\/chronic pelvic pain syndrome using the UPOINT system. World J Urol 31(4):755\u2013760. https:\/\/doi.org\/10.1007\/s00345-013-1075-6","journal-title":"World J Urol"},{"key":"5514_CR4","doi-asserted-by":"crossref","unstructured":"Bryk DJ, Shoskes DA (2021) Using the UPOINT system to manage men with chronic pelvic pain syndrome. Arab J Urol. Jul 23;19(3):387\u2013393. Doi: 10.1080\/2090598X.2021.1955546. PMID: 34552790; PMCID: PMC8451687","DOI":"10.1080\/2090598X.2021.1955546"},{"issue":"5","key":"5514_CR5","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1016\/j.amjmed.01.043","volume":"121","author":"BC Taylor","year":"2008","unstructured":"Taylor BC, Noorbaloochi S, McNaughton-Collins M, Saigal CS, Sohn MW, Pontari MA, Litwin MS, Wilt TJ, Urologic Diseases in America Project (2008) Excessive antibiotic use in men with prostatitis. Am J Med 121(5):444\u2013449. 10.1016\/j. amjmed. 01.043","journal-title":"Am J Med"},{"issue":"1","key":"5514_CR6","doi-asserted-by":"publisher","first-page":"13588","DOI":"10.1038\/s41598-020-70491-3","volume":"10","author":"A Latorre-P\u00e9rez","year":"2020","unstructured":"Latorre-P\u00e9rez A, Villalba-Bermell P, Pascual J, Vilanova C (2020) Assembly methods for nanopore-based metagenomic sequencing: a comparative study. Sci Rep 10(1):13588. https:\/\/doi.org\/10.1038\/s41598-020-70491-3","journal-title":"Sci Rep"},{"key":"5514_CR7","doi-asserted-by":"crossref","unstructured":"Hu, C., Yang, H., Zhao, Y., Chen, X., Dong, Y., Li, L.,\u2026 Dai, J. (2016). The role of inflammatory cytokines and ERK1\/2 signaling in chronic prostatitis\/chronic pelvic pain syndrome with related mental health disorders. Scientific Reports, 6(1), 28608. https:\/\/doi.org\/10.1038\/srep28608","DOI":"10.1038\/srep28608"},{"issue":"1","key":"5514_CR8","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1093\/clinchem\/hvab173","volume":"68","author":"S Miller","year":"2022","unstructured":"Miller S, Chiu C (2022) The role of metagenomics and next-generation sequencing in infectious disease diagnosis. Clin Chem 68(1):115\u2013124. https:\/\/doi.org\/10.1093\/clinchem\/hvab173","journal-title":"Clin Chem"},{"key":"5514_CR9","doi-asserted-by":"publisher","unstructured":"Nickel JC, Alexander RB, Schaeffer AJ, Landis JR, Knauss JS, Propert KJ, Chronic Prostatitis Collaborative Research Network Study Group (2003) &. Leukocytes and bacteria in men with chronic prostatitis\/chronic pelvic pain syndrome compared to asymptomatic controls. The Journal of urology. 170(3), 818\u2013822. https:\/\/doi.org\/10.1097\/01.ju.0000082252.49374.e9. PMID: 12913707","DOI":"10.1097\/01.ju.0000082252.49374.e9"},{"issue":"8","key":"5514_CR10","doi-asserted-by":"publisher","first-page":"1615","DOI":"10.3390\/microorganisms9081615","volume":"9","author":"A Torres-S\u00e1nchez","year":"2021","unstructured":"Torres-S\u00e1nchez A, Pardo-Cacho J, L\u00f3pez-Moreno A, Ruiz-Moreno \u00c1, Cerk K, Aguilera M (2021) Antimicrobial effects of potential probiotics of Bacillus spp. Isolated from human microbiota: in Vitro and in Silico methods. Microorganisms 9(8):1615. https:\/\/doi.org\/10.3390\/microorganisms9081615","journal-title":"Microorganisms"},{"issue":"9","key":"5514_CR11","doi-asserted-by":"publisher","first-page":"2138","DOI":"10.1016\/j.drudis.2021.04.016","volume":"26","author":"L Maryam","year":"2021","unstructured":"Maryam L, Usmani SS, Raghava GP (2021) Computational resources in the management of antibiotic resistance: speeding up drug discovery. Drug Discovery Today 26(9):2138\u20132151","journal-title":"Drug Discovery Today"},{"issue":"D1","key":"5514_CR12","doi-asserted-by":"publisher","first-page":"D678","DOI":"10.1093\/nar\/gkac1003","volume":"51","author":"RD Olson","year":"2023","unstructured":"Olson RD, Assaf R, Brettin T, Conrad N, Cucinell C, Davis JJ, Stevens RL (2023) Introducing the bacterial and viral bioinformatics resource center (BV-BRC): a resource combining PATRIC, IRD and ViPR. Nucleic Acids Res 51(D1):D678\u2013D689","journal-title":"Nucleic Acids Res"},{"issue":"7","key":"5514_CR13","doi-asserted-by":"publisher","first-page":"1697","DOI":"10.1007\/s00467-020-04742-w","volume":"36","author":"J Diviney","year":"2021","unstructured":"Diviney J, Jaswon MS (2021) Urine collection methods and dipstick testing in non-toilet-trained children. Pediatr Nephrol 36(7):1697\u20131708. https:\/\/doi.org\/10.1007\/s00467-020-04742-w","journal-title":"Pediatr Nephrol"},{"key":"5514_CR14","volume-title":"Campbell-Walsh-Wein Urology","author":"EP Castle","year":"2021","unstructured":"Castle EP, Wolter CE, Woods ME (2021) Evaluation of the urologic patient: testing and imaging. In: Partin AW, Dmochowski RR, Kavoussi LR, Peters CA (eds) Campbell-Walsh-Wein Urology, 12th edn. Elsevier, Philadelphia, PA. Chap. 2.","edition":"12"},{"issue":"3","key":"5514_CR15","doi-asserted-by":"publisher","first-page":"e0264291","DOI":"10.1371\/journal.pone.0264291","volume":"17","author":"C Baek","year":"2022","unstructured":"Baek C, Kim WJ, Moon J, Moon SY, Kim W, Hu HJ, Min J (2022) Differences in the gut microbiome composition of Korean children and adult samples based on different DNA isolation kits. PLoS ONE 17(3):e0264291. https:\/\/doi.org\/10.1371\/journal.pone.0264291","journal-title":"PLoS ONE"},{"issue":"4","key":"5514_CR16","doi-asserted-by":"publisher","first-page":"491","DOI":"10.33073\/pjm-2022-042","volume":"71","author":"TM Potindji","year":"2022","unstructured":"Potindji TM, Momani OA, Omowumi BB, Baddal B (2022) Screening of toxin genes in Methicillin-Resistant Staphylococcus aureus Clinical isolates from a hospital setting in a Tertiary Hospital in Northern Cyprus. Pol J Microbiol 71(4):491. https:\/\/doi.org\/10.33073\/pjm-2022-042","journal-title":"Pol J Microbiol"},{"key":"5514_CR17","doi-asserted-by":"publisher","unstructured":"Lee J, Kerkhof (2021) Is Oxford Nanopore sequencing ready for analyzing complex microbiomes? FEMS Microbiology Ecology. 97(3):fiab001. https:\/\/doi.org\/10.1093\/femsec\/fiab001","DOI":"10.1093\/femsec\/fiab001"},{"key":"5514_CR18","doi-asserted-by":"publisher","first-page":"806476","DOI":"10.3389\/fcimb.2021.806476","volume":"11","author":"M Rozas","year":"2022","unstructured":"Rozas M, Brillet F, Callewaert C, Paetzold B (2022) MinION\u2122 nanopore sequencing of skin microbiome 16S and 16S-23S rRNA gene amplicons. Front Cell Infect Microbiol 11:806476. https:\/\/doi.org\/10.3389\/fcimb.2021.806476","journal-title":"Front Cell Infect Microbiol"},{"issue":"11","key":"5514_CR19","doi-asserted-by":"publisher","first-page":"e0224650","DOI":"10.1371\/journal.pone.0224650","volume":"14","author":"K Bhatnagar","year":"2019","unstructured":"Bhatnagar K, Wong A (2019) The mutational landscape of quinolone resistance in Escherichia coli. PLoS ONE 14(11):e0224650. https:\/\/doi.org\/10.1371\/journal.pone.0224650","journal-title":"PLoS ONE"},{"key":"5514_CR20","doi-asserted-by":"publisher","unstructured":"Linda Kherroubi J, Bacon (2024) Navigating fluoroquinolone resistance in Gram-negative bacteria: a comprehensive evaluation, JAC-Antimicrobial resistance, 6, issue 4. August dlae127 https:\/\/doi.org\/10.1093\/jacamr\/dlae127. Khondaker Miraz Rahman","DOI":"10.1093\/jacamr\/dlae127"},{"key":"5514_CR21","doi-asserted-by":"publisher","unstructured":"Torres-Puig S, Garc\u00eda V, St\u00e6rk K, Andersen TE, M\u00f8ller-Jensen J, Olsen JE, Herrero-Fresno A (2022) Omics technologies-what have they told us about uropathogenic Escherichia coli fitness and virulence during urinary tract infection? Frontiers in cellular and infection microbiology. 12:824039. https:\/\/doi.org\/10.3389\/fcimb.2022.824039","DOI":"10.3389\/fcimb.2022.824039"},{"issue":"9","key":"5514_CR22","doi-asserted-by":"publisher","first-page":"794","DOI":"10.3390\/pathogens13090794","volume":"13","author":"K Sung","year":"2024","unstructured":"Sung K, Nawaz M, Park M, Chon J, Khan SA, Alotaibi K, Khan AA (2024) Comprehensive genomic analysis of Uropathogenic E. Coli: virulence factors, Antimicrobial Resistance, and Mobile Genetic Elements. Pathogens 13(9):794. https:\/\/doi.org\/10.3390\/pathogens13090794","journal-title":"Pathogens"},{"key":"5514_CR23","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/978-1-0716-3072-3_3","volume-title":"Metagenomic Data Analysis","author":"D Wang","year":"2023","unstructured":"Wang D (2023) Metagenomics Databases for Bacteria. Metagenomic Data Analysis. Springer US, New York, NY, pp 55\u201367. https:\/\/doi.org\/10.1007\/978-1-0716-3072-3_3"},{"key":"5514_CR24","doi-asserted-by":"publisher","first-page":"303","DOI":"10.3354\/meps07841","volume":"372","author":"PJ Somerfield","year":"2008","unstructured":"Somerfield PJ (2008) Identification of the Bray-Curtis similarity index: comment on Yoshioka. Mar Ecol Prog Ser 372:303\u2013306. https:\/\/doi.org\/10.3354\/meps07841","journal-title":"Mar Ecol Prog Ser"},{"key":"5514_CR25","doi-asserted-by":"publisher","unstructured":"MacFarland TW, Yates JM, MacFarland TW, Yates JM (2016) Mann\u2013Whitney u test. Introduction Nonparametric Stat Biol Sci Using R 103\u2013132. https:\/\/doi.org\/10.1007\/978-3-319-30634-6_4","DOI":"10.1007\/978-3-319-30634-6_4"},{"issue":"1","key":"5514_CR26","doi-asserted-by":"publisher","first-page":"3","DOI":"10.5213\/inj.2040174.087","volume":"25","author":"MS Kim","year":"2021","unstructured":"Kim MS, Jung SI (2021) The urinary tract microbiome in male genitourinary diseases: focusing on benign prostate hyperplasia and lower urinary tract symptoms. Int Neurourol J 25(1):3. https:\/\/doi.org\/10.5213\/inj.2040174.087","journal-title":"Int Neurourol J"},{"issue":"2","key":"5514_CR27","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.prnil.2022.03.004","volume":"10","author":"M Miyake","year":"2022","unstructured":"Miyake M, Tatsumi Y, Ohnishi K, Fujii T, Nakai Y, Tanaka N, Fujimoto K (2022) Prostate diseases and microbiome in the prostate, gut, and urine. Prostate Int 10(2):96\u2013107. https:\/\/doi.org\/10.1016\/j.prnil.2022.03.004","journal-title":"Prostate Int"},{"issue":"1\u20132","key":"5514_CR28","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S0005-2736(03)00029-4","volume":"1611","author":"C Carrillo","year":"2003","unstructured":"Carrillo C, Teruel JA, Aranda FJ, Ortiz A (2003) Molecular mechanism of membrane permeabilization by the peptide antibiotic surfactin. Biochim et Biophys Acta (BBA)-Biomembranes 1611(1\u20132):91\u201397. https:\/\/doi.org\/10.1016\/S0005-2736(03)00029-4","journal-title":"Biochim et Biophys Acta (BBA)-Biomembranes"},{"issue":"1","key":"5514_CR29","doi-asserted-by":"publisher","first-page":"39303","DOI":"10.1038\/srep39303","volume":"6","author":"P Gan","year":"2016","unstructured":"Gan P, Gao Z, Zhao X, Qi G (2016) Surfactin inducing mitochondria-dependent ROS to activate MAPKs, NF-\u03baB and inflammasomes in macrophages for adjuvant activity. Sci Rep 6(1):39303. https:\/\/doi.org\/10.1038\/srep39303","journal-title":"Sci Rep"},{"key":"5514_CR30","doi-asserted-by":"crossref","unstructured":"Min, J. K., Kim, Y. M., Kim, S. W., Kwon, M. C., Kong, Y. Y., Hwang, I. K.,\u2026 Kwon,Y. G. (2005). TNF-related activation-induced cytokine enhances leukocyte adhesiveness:induction of ICAM-1 and VCAM-1 via TNF receptor-associated factor and protein kinase C-dependent NF-\u03baB activation in endothelial cells. The Journal of Immunology, 175(1), 531\u2013540. https:\/\/doi.org\/10.4049\/jimmunol.175.1.531","DOI":"10.4049\/jimmunol.175.1.531"},{"key":"5514_CR31","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1186\/1471-230X-9-53","volume":"9","author":"I Treede","year":"2009","unstructured":"Treede I, Braun A, Jeliaskova P et al (2009) TNF-\u03b1-induced up-regulation of pro-inflammatory cytokines is reduced by phosphatidylcholine in intestinal epithelial cells. BMC Gastroenterol 9:53. https:\/\/doi.org\/10.1186\/1471-230X-9-53","journal-title":"BMC Gastroenterol"},{"issue":"7","key":"5514_CR32","doi-asserted-by":"publisher","first-page":"2667","DOI":"10.1529\/biophysj.107.114090","volume":"94","author":"M Deleu","year":"2008","unstructured":"Deleu M, Paquot M, Nylander T (2008) Effect of fengycin, a lipopeptide produced by Bacillus subtilis, on model biomembranes. Biophys J 94(7):2667\u20132679","journal-title":"Biophys J"},{"issue":"10","key":"5514_CR33","doi-asserted-by":"publisher","first-page":"1587","DOI":"10.3390\/microorganisms8101587","volume":"8","author":"JY Yoo","year":"2020","unstructured":"Yoo JY, Groer M, Dutra SVO, Sarkar A, McSkimming DI (2020) Gut microbiota and immune system interactions. Microorganisms 8(10):1587. https:\/\/doi.org\/10.3390\/microorganisms8101587","journal-title":"Microorganisms"},{"issue":"10","key":"5514_CR34","doi-asserted-by":"publisher","first-page":"934","DOI":"10.3390\/antiox9100934","volume":"9","author":"MM Khan","year":"2020","unstructured":"Khan MM, Kim YK, Bilkis T, Suh JW, Lee DY, Yoo JC (2020) Reduction of oxidative stress through activating the Nrf2 mediated HO-1 antioxidant efficacy signaling pathway by MS15, an antimicrobial peptide from Bacillus velezensis. Antioxidants 9(10):934. https:\/\/doi.org\/10.3390\/antiox9100934","journal-title":"Antioxidants"},{"issue":"5","key":"5514_CR35","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1016\/j.jaci.2004.07.060","volume":"114","author":"CE Reed","year":"2004","unstructured":"Reed CE, Kita H (2004) The role of protease activation of inflammation in allergic respiratory diseases. J Allergy Clin Immunol 114(5):997\u20131009. https:\/\/doi.org\/10.1016\/j.jaci.2004.07.060","journal-title":"J Allergy Clin Immunol"},{"key":"5514_CR36","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.jbiotec.2014.11.033","volume":"194","author":"Y Zhao","year":"2015","unstructured":"Zhao Y, Sangare L, Wang Y, Folly YME, Selvaraj JN, Xing F, Liu Y (2015) Complete genome sequence of Bacillus subtilis SG6 antagonistic against Fusarium Graminearum. J Biotechnol 194:10\u201311. https:\/\/doi.org\/10.1016\/j.jbiotec.2014.11.033","journal-title":"J Biotechnol"},{"key":"5514_CR37","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/j.jbiotec.2016.06.013","volume":"231","author":"M Kr\u00f6ber","year":"2016","unstructured":"Kr\u00f6ber M, Verwaaijen B, Wibberg D, Winkler A, P\u00fchler A, Schl\u00fcter A (2016) Comparative transcriptome analysis of the biocontrol strain Bacillus amyloliquefaciens FZB42 as response to biofilm formation analyzed by RNA sequencing. J Biotechnol 231:212\u2013223. https:\/\/doi.org\/10.1016\/j.jbiotec.2016.06.013","journal-title":"J Biotechnol"},{"key":"5514_CR38","doi-asserted-by":"publisher","unstructured":"Priadko, K., Romano, L., Olivieri, S., Romeo, M., Barone, B., Sciorio, C.,\u2026 Napolitano,L. (2022). Intestinal microbiota, intestinal permeability and the urogenital tract:is there a pathophysiological link? Journal of Physiology & Pharmacology, 73(5). DOI: https:\/\/doi.org\/10.26402\/jpp.2022.5.01","DOI":"10.26402\/jpp.2022.5.01"},{"key":"5514_CR39","unstructured":"Pendegast H, Leslie S, Rosario D (2024) Chronic prostatitis and chronic pelvic pain syndrome in men. StatPearls"},{"key":"5514_CR40","doi-asserted-by":"publisher","unstructured":"Shah H, Bakthavachalam V, Motika S, Hergenrother P, Munirathinam G (2021) Evaluation of Ferroptocide (FTC) as a potential immunogenic cell death inducing Agent in prostate Cancer cells. FASEB J 35. https:\/\/doi.org\/10.1096\/fasebj.2021.35.S1.03356","DOI":"10.1096\/fasebj.2021.35.S1.03356"},{"issue":"6","key":"5514_CR41","first-page":"490","volume":"12","author":"H Wang","year":"2006","unstructured":"Wang H, Chen ZH, Zhu YY, Wang T, Wu XJ (2006) Penetrability and therapeutic effect of Vancomycin to the prostates of rats with bacterial prostatitis (BP) or BPH-BP. Zhonghua Nan Ke xue\u2009=\u2009National. J Androl 12(6):490\u2013495","journal-title":"J Androl"},{"issue":"6","key":"5514_CR42","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1002\/jcph.1800","volume":"61","author":"T Onita","year":"2021","unstructured":"Onita T, Ikawa K, Nakamura K, Nishikawa G, Kobayashi I, Ishihara N, Tamaki H, Yano T, Naora K, Morikawa N (2021) Prostatic Pharmacokinetic\/Pharmacodynamic Evaluation of Ampicillin-Sulbactam for Bacterial Prostatitis and Preoperative Prophylaxis. J Clin Pharmacol 61(6):820\u2013831. https:\/\/doi.org\/10.1002\/jcph.1800","journal-title":"J Clin Pharmacol"},{"issue":"7","key":"5514_CR43","doi-asserted-by":"publisher","first-page":"2355","DOI":"10.1128\/aac.48.7.2355-2363.2004","volume":"48","author":"DD Levy","year":"2004","unstructured":"Levy DD, Sharma B, Cebula TA (2004) Single-nucleotide polymorphism mutation spectra and resistance to quinolones in Salmonella enterica Serovar Enteritidis with a mutator phenotype. Antimicrob Agents Chemother 48(7):2355\u20132363. https:\/\/doi.org\/10.1128\/aac.48.7.2355-2363.2004","journal-title":"Antimicrob Agents Chemother"}],"container-title":["World Journal of Urology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00345-025-05514-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00345-025-05514-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00345-025-05514-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T13:50:41Z","timestamp":1767966641000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00345-025-05514-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,26]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["5514"],"URL":"https:\/\/doi.org\/10.1007\/s00345-025-05514-7","relation":{},"ISSN":["1433-8726"],"issn-type":[{"value":"1433-8726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,26]]},"assertion":[{"value":"25 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 February 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"188"}}