{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T20:33:46Z","timestamp":1778790826192,"version":"3.51.4"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Georgius Agricola Stiftung Ruhr","award":["na"],"award-info":[{"award-number":["na"]}]},{"name":"Universit\u00e4tsklinikum Knappschaftskrankenhaus Bochum GmbH"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ophthalmologie"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:sec>\n                <jats:title>Hintergrund<\/jats:title>\n                <jats:p>Retinale Isch\u00e4mie spielt bei zahlreichen Augenerkrankungen wie beispielsweise dem Glaukom eine zentrale pathophysiologische Rolle. Neben der Apoptose z\u00e4hlen Autophagie, Nekroptose und Ferroptose zu den Zelltodmechanismen, die der Isch\u00e4mie nachgeschaltet sind. Ihre Rolle ist jedoch nicht eindeutig gekl\u00e4rt und wird kontrovers diskutiert.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Ziel der Arbeit<\/jats:title>\n                <jats:p>Ziel dieser Studie ist es, ein verbessertes Verst\u00e4ndnis \u00fcber die Rolle alternativer Zelltodmechanismen wie Autophagie und Nekroptose im Tiermodell der Isch\u00e4mie-Reperfusion zu erhalten. Diese Erkenntnisse k\u00f6nnten als Basis f\u00fcr einen autophagie- oder nekroptosebasierten Therapieansatz dienen.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Material und Methoden<\/jats:title>\n                <jats:p>Die retinale Isch\u00e4mie wurde bei 6 bis 8\u00a0Wochen alten Ratten in einem Auge durch das vorr\u00fcbergehende Erh\u00f6hen des Augeninnendrucks auf 140\u202fmm\u202fHg induziert (60\u202fmin), gefolgt von der Reperfusion. Das unbehandelte kontralaterale Auge diente als Kontrolle. Die Retinae wurden 7\u00a0Tage nach Isch\u00e4mie-Reperfusion immunhistochemisch mit Markern f\u00fcr retinale Ganglienzellen (RGZ), Makroglia (GFAP) sowie einem Autophagiemarker (LAMP1) und einem Nekroptosemarker (RIPK3) untersucht (<jats:italic>n<\/jats:italic>\u202f=\u20096\/Gruppe).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Ergebnisse<\/jats:title>\n                <jats:p>Die Isch\u00e4mie-Reperfusion f\u00fchrte nach 7\u00a0Tagen sowohl zu einem signifikanten RGZ-Verlust (<jats:italic>p<\/jats:italic>\u202f\u2264\u20090,001) als auch zu einer signifikanten Zunahme der Astrozytenfl\u00e4che (<jats:italic>p<\/jats:italic>\u202f=\u20090,026). Interessanterweise war die Anzahl autophagischer LAMP1-positiver Zellen 7\u00a0Tage nach Isch\u00e4mie unver\u00e4ndert (<jats:italic>p<\/jats:italic>\u202f=\u20090,272), wohingegen die Anzahl nekroptotischer RIPK3-positiver Zellen signifikant erh\u00f6ht vorlag (<jats:italic>p<\/jats:italic>\u202f\u2264\u20090,001).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Diskussion<\/jats:title>\n                <jats:p>Sieben Tage nach Isch\u00e4mie-Reperfusion scheinen nekroptotische Prozesse aktiviert zu sein und so zum retinalen Zelltod und zur Aktivierung der Astrozyten beizutragen. Sp\u00e4te autophagische Prozesse sind 7\u00a0Tage nach Isch\u00e4mie nicht aktiviert. Nekroptose-assoziierte Parameter k\u00f6nnten daher als fr\u00fchzeitiger therapeutischer Ansatz nach Isch\u00e4mie in Betracht gezogen werden.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00347-024-02063-z","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T14:04:45Z","timestamp":1719410685000},"page":"644-649","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Zellverlust bei retinaler Isch\u00e4mie ist assoziiert mit vermehrter Nekroptose","Cell loss in retinal ischemia is associated with increased necroptosis"],"prefix":"10.1007","volume":"121","author":[{"given":"Teresa","family":"Tsai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonie","family":"Deppe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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Burkhard Dick falsch strukturiert. \u201aH. Burkhard\u2018 ist korrekterweise der Vorname und \u201aDick\u2018 der Familienname.","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Einhaltung ethischer Richtlinien"}},{"value":"T.\u00a0Tsai, L.\u00a0Deppe, H.\u00a0B.\u00a0Dick und S.C.\u00a0Joachim geben an, dass kein Interessenkonflikt besteht.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Interessenkonflikt"}},{"value":"Alle nationalen Richtlinien zur Haltung und zum Umgang mit Labortieren wurden eingehalten, und die notwendigen Zustimmungen der zust\u00e4ndigen Beh\u00f6rden liegen vor.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Interessenkonflikt"}}]}}