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Although cytoskeletal alterations are linked to HF, the tubulin signature of common experimental models has been incompletely defined. Here, we assessed the tubulin signature in a large set of human cardiac samples and myocardium of animal models with cardiac remodeling caused by pressure overload, myocardial infarction or a gene defect. We studied levels of total, acetylated, and detyrosinated \u03b1-tubulin and desmin in cardiac tissue from hypertrophic (HCM) and dilated cardiomyopathy (DCM) patients with an idiopathic (<jats:italic>n<\/jats:italic>\u2009=\u20097), ischemic (<jats:italic>n<\/jats:italic>\u2009=\u20097) or genetic origin (<jats:italic>n<\/jats:italic>\u2009=\u200959), and in a pressure-overload concentric hypertrophic pig model (<jats:italic>n<\/jats:italic>\u2009=\u200932), pigs with a myocardial infarction (<jats:italic>n<\/jats:italic>\u2009=\u200928), mature pigs (<jats:italic>n<\/jats:italic>\u2009=\u20096), and mice (<jats:italic>n<\/jats:italic>\u2009=\u200915) carrying the HCM-associated <jats:italic>MYBPC3<\/jats:italic><jats:sub>2373insG<\/jats:sub> mutation. In the human samples, detyrosinated \u03b1-tubulin was increased 4-fold in end-stage HCM and 14-fold in pediatric DCM patients. Acetylated \u03b1-tubulin was increased twofold in ischemic patients. Across different animal models, the tubulin signature remained mostly unaltered. Only mature pigs were characterized by a 0.5-fold decrease in levels of total, acetylated, and detyrosinated \u03b1-tubulin. Moreover, we showed increased desmin levels in biopsies from NYHA class II HCM patients (2.5-fold) and the pressure-overload pig model (0.2\u20130.3-fold). Together, our data suggest that desmin levels increase early on in concentric hypertrophy and that animal models only partially recapitulate the proliferated and modified tubulin signature observed clinically. Our data warrant careful consideration when studying maladaptive responses to changes in the tubulin content in animal models.<\/jats:p>\n                <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s00360-023-01509-1","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T20:29:55Z","timestamp":1693340995000},"page":"581-595","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The microtubule signature in cardiac disease: etiology, disease stage, and age dependency"],"prefix":"10.1007","volume":"193","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3513-8643","authenticated-orcid":false,"given":"S\u0131la","family":"Alg\u00fcl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa M.","family":"Dorsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oana","family":"Sorop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aryan","family":"Vink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Michels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristobal G.","family":"dos Remedios","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiel","family":"Dalinghaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daphne","family":"Merkus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk J.","family":"Duncker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diederik W. 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