{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:26:52Z","timestamp":1787682412241,"version":"build-2784847793"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T00:00:00Z","timestamp":1782518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T00:00:00Z","timestamp":1782518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade Estadual Paulista J\u00falio De Mesquita Filho"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Comp Physiol B"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1007\/s00360-026-01671-2","type":"journal-article","created":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T05:25:48Z","timestamp":1782537948000},"page":"499-508","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Carbohydrate handling in frugivorous tambaqui (Colossoma macropomum): enzymatic and glycemic responses to dietary sugars"],"prefix":"10.1007","volume":"196","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3274-2012","authenticated-orcid":false,"given":"Gabriela Castellani","family":"Carli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1204-1718","authenticated-orcid":false,"given":"Thaise Mota","family":"S\u00e1tiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4645-2996","authenticated-orcid":false,"given":"Douglas","family":"de Souza Graciano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9051-4213","authenticated-orcid":false,"given":"Maria Karolaine Moriman","family":"Delgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3982-3912","authenticated-orcid":false,"given":"Affonso Gama Souza","family":"Pinheiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4382-9575","authenticated-orcid":false,"given":"Cristiele","family":"da Silva Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5820-188X","authenticated-orcid":false,"given":"Leonardo Susumu","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,27]]},"reference":[{"key":"1671_CR3","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.14060","author":"GC Carli","year":"2024","unstructured":"Carli GC, Palma M, S\u00e1tiro TM, Soares HHD, Ferrari JEC, Viegas I, Takahashi LS (2024) The protein\/carbohydrate ratio in the diet affects zootechnical performance and the regulation of intermediary metabolism in juveniles tambaqui (Colossoma macropomum). J Anim Physiol Anim Nutr (Berl). https:\/\/doi.org\/10.1111\/jpn.14060","journal-title":"J Anim Physiol Anim Nutr (Berl)"},{"key":"1671_CR4","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1016\/0006-291X(67)90369-5","volume":"28","author":"HC Chang","year":"1967","unstructured":"Chang HC, Seidman TG, Lane DM (1967) Liver acetyl-CoA-carboxylase and fatty synthase relative activities in the normal state and in hereditary obesity. Biochim Biophys Res Commun 28:682\u2013686. https:\/\/doi.org\/10.1016\/0006-291X(67)90369-5","journal-title":"Biochim Biophys Res Commun"},{"key":"1671_CR5","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2019.734538","volume":"515","author":"JX Chen","year":"2020","unstructured":"Chen JX, Feng JY, Zhu J, Luo L, Lin SM, Wang DS et al (2020) Starch to protein ratios in practical diets for genetically improved farmed Nile tilapia Oreochromis niloticus: effects on growth, body composition, peripheral glucose metabolism and glucose tolerance. Aquaculture 515:734538. https:\/\/doi.org\/10.1016\/j.aquaculture.2019.734538","journal-title":"Aquaculture"},{"key":"1671_CR34","doi-asserted-by":"publisher","unstructured":"Eames SC, Philipson LH, Prince VE, Kinkel MD. Blood sugar measurement in zebrafish reveals dynamics of glucose homeostasis. Zebrafish. 2010 Jun;7(2):205-13. https:\/\/doi.org\/10.1089\/zeb.2009.0640","DOI":"10.1089\/zeb.2009.0640"},{"key":"1671_CR7","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1007\/s10695-008-9259-5","volume":"35","author":"P Enes","year":"2009","unstructured":"Enes P, Panserat S, Kaushik S, Oliva-Teles A (2009) Nutritional regulation of hepatic glucose metabolism in fish. Fish Physiol Biochem 35:519\u2013539. https:\/\/doi.org\/10.1007\/s10695-008-9259-5","journal-title":"Fish Physiol Biochem"},{"key":"1671_CR8","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2021.115020","volume":"279","author":"CVT Farias","year":"2021","unstructured":"Farias CVT, Oshiro AM, Sousa LS, Almeida VNS, Takahashi LS (2021) Amylose and amylopectin levels affect the growth performance and metabolism in pacu Piaractus mesopotamicus. Anim Feed Sci Technol 279:115020. https:\/\/doi.org\/10.1016\/j.anifeedsci.2021.115020","journal-title":"Anim Feed Sci Technol"},{"key":"1671_CR9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2021.100928","volume":"41","author":"JEC Ferrari","year":"2022","unstructured":"Ferrari JEC, Palma M, Carli G, S\u00e1tiro TM, Tavares LC, Viegas I, Takahashi LS (2022) Carbohydrate tolerance in Amazon tambaqui (Colossoma macropomum) revealed by NMR-metabolomics: are glucose and fructose different sugars for fruit-eating fish? Comp Biochem Physiol D Genomics Proteomics 41:100928. https:\/\/doi.org\/10.1016\/j.cbd.2021.100928","journal-title":"Comp Biochem Physiol D Genomics Proteomics"},{"key":"1671_CR10","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1093\/clinchem\/18.7.673","volume":"18","author":"CS Frings","year":"1972","unstructured":"Frings CS, Fendley TW, Dunn RT, Queen CA (1972) Improved determination of total lipids by the sulpho-phospho-vanillin reaction. Clin Chem 18:673\u2013674. https:\/\/doi.org\/10.1093\/clinchem\/18.7.673","journal-title":"Clin Chem"},{"key":"1671_CR11","first-page":"398","volume":"29","author":"S Fu","year":"2005","unstructured":"Fu S, Xie X (2005) Effect of dietary carbohydrate levels on growth performance in Silurus meridionalis Chen. Acta Hydrobiol Sin 29:398","journal-title":"Acta Hydrobiol Sin"},{"key":"1671_CR12","doi-asserted-by":"publisher","first-page":"R609","DOI":"10.1152\/ajpregu.1991.261.3.R609","volume":"261","author":"JS Harmon","year":"1991","unstructured":"Harmon JS, Eilertson CD, Sheridan MA, Plisetskaya EM (1991) Insulin suppression is associated with hypersomatostatinemia and hyperglucagonemia in glucose-injected rainbow trout. Am J Physiol Regul Integr Comp Physiol 261:R609\u2013R613. https:\/\/doi.org\/10.1152\/ajpregu.1991.261.3.R609","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"1671_CR13","doi-asserted-by":"publisher","first-page":"993","DOI":"10.1111\/raq.12638","volume":"14","author":"AWS Hilsdorf","year":"2022","unstructured":"Hilsdorf AWS, Hallerman E, Vallad\u00e3o EMR et al (2022) The farming and husbandry of Colossoma macropomum: from Amazonian waters to sustainable production. Rev Aquac 14:993\u20131027. https:\/\/doi.org\/10.1111\/raq.12638","journal-title":"Rev Aquac"},{"key":"1671_CR15","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.aquaculture.2016.02.007","volume":"467","author":"BS Kalamalan","year":"2017","unstructured":"Kalamalan BS, M\u00e9dale F, Panserat S (2017) Utilisation of dietary carbohydrates in farmed fishes: new insights on influencing factors, biological limitations and future strategies. Aquaculture 467:3\u201327. https:\/\/doi.org\/10.1016\/j.aquaculture.2016.02.007","journal-title":"Aquaculture"},{"key":"1671_CR16","doi-asserted-by":"publisher","first-page":"3735","DOI":"10.1111\/are.13841","volume":"49","author":"Y Liu","year":"2018","unstructured":"Liu Y, Chen Q, Tan B, Dong X, Yang Q, Zhang S, Chen L (2018) Effects of dietary carbohydrate levels on growth, glucose tolerance, glucose homeostasis and GLUT4 gene expression in Tilapia nilotica. Aquac Res 49:3735\u20133745. https:\/\/doi.org\/10.1111\/are.13841","journal-title":"Aquac Res"},{"key":"1671_CR17","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1016\/j.physbeh.2008.12.015","volume":"96","author":"JF L\u00f3pez-Olmeda","year":"2009","unstructured":"L\u00f3pez-Olmeda JF, Egea-Alvarez M, S\u00e1nchez-V\u00e1zquez FJ (2009) Glucose tolerance in fish: is the daily feeding time important? Physiol Behav 96:631\u2013636. https:\/\/doi.org\/10.1016\/j.physbeh.2008.12.015","journal-title":"Physiol Behav"},{"key":"1671_CR18","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2023.1310415","volume":"10","author":"Q Ma","year":"2023","unstructured":"Ma Q, Xu H, Limbu SM, Wei Y, Liang M (2023) Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance. Front Mar Sci 10:1310415. https:\/\/doi.org\/10.3389\/fmars.2023.1310415","journal-title":"Front Mar Sci"},{"key":"1671_CR19","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/S1096-4959(01)00316-5","volume":"129","author":"TW Moon","year":"2001","unstructured":"Moon TW (2001) Glucose intolerance in teleost fish: fact or fiction? Comp Biochem Physiol B Biochem Mol Biol 129:243\u2013249. https:\/\/doi.org\/10.1016\/S1096-4959(01)00316-5","journal-title":"Comp Biochem Physiol B Biochem Mol Biol"},{"key":"1671_CR20","doi-asserted-by":"publisher","first-page":"2189","DOI":"10.1139\/z89-309","volume":"67","author":"TW Moon","year":"1989","unstructured":"Moon TW, Foster GD, Plisetskaya EM (1989) Changes in peptide hormones and liver enzymes in the rainbow trout deprived of food 6 weeks. Can J Zool 67:2189\u20132193. https:\/\/doi.org\/10.1139\/z89-309","journal-title":"Can J Zool"},{"key":"1671_CR21","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/0300-9629(90)90014-J","volume":"95","author":"AE Morales","year":"1990","unstructured":"Morales AE, Garcia L, Higuera M (1990) Influence of handling and\/or anesthesia on stress response in rainbow trout: effects on liver primary metabolism. Comp Biochem Physiol A 95:87\u201393. https:\/\/doi.org\/10.1016\/0300-9629(90)90014-J","journal-title":"Comp Biochem Physiol A"},{"key":"1671_CR22","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.cca.2004.10.008","volume":"139","author":"AE Morales","year":"2004","unstructured":"Morales AE, P\u00e9rez-Jim\u00e9nez A, Hidalgo MC, Abell\u00e1n E, Cardenete G (2004) Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comp Biochem Physiol A Mol Integr Physiol 139:153\u2013161. https:\/\/doi.org\/10.1016\/j.cca.2004.10.008","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"key":"1671_CR24","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/S0304-4165(99)00213-5","volume":"1474","author":"S Panserat","year":"2000","unstructured":"Panserat S, Blin C, M\u00e9dale F, Plagnes-Juan E, Breque J, Krishnamoorthy J, Kaushik S (2000) Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio). Biochim Biophys Acta Gen Subj 1474:61\u201369. https:\/\/doi.org\/10.1016\/S0304-4165(99)00213-5","journal-title":"Biochim Biophys Acta Gen Subj"},{"key":"1671_CR25","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1111\/j.1095-8649.2001.tb00144.x","volume":"59","author":"S Panserat","year":"2001","unstructured":"Panserat S, Capilla E, Gutierrez J, Vachot C, Plagnes-Juan E, Aguirre P et al (2001) Dietary fructose does not specifically induce hepatic glucokinase expression in rainbow trout. J Fish Biol 59:455\u2013458. https:\/\/doi.org\/10.1111\/j.1095-8649.2001.tb00144.x","journal-title":"J Fish Biol"},{"key":"1671_CR27","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1007\/s00360-012-0658-7","volume":"182","author":"S Polakof","year":"2012","unstructured":"Polakof S, Panserat S, Soengas JL, Moon TW (2012) Glucose metabolism in fish: a review. J Comp Physiol B 182:1015\u20131045. https:\/\/doi.org\/10.1007\/s00360-012-0658-7","journal-title":"J Comp Physiol B"},{"key":"1671_CR28","doi-asserted-by":"publisher","unstructured":"Reinhold JG (1953) Total protein, albumin and globulin. In: Reiner M (ed) Standard methods of clinical chemistry, vol 1. Academic Press, pp 88. https:\/\/doi.org\/10.1016\/B978-0-12-609101-4.50019-8","DOI":"10.1016\/B978-0-12-609101-4.50019-8"},{"key":"1671_CR35","doi-asserted-by":"publisher","unstructured":"Shikata, T., Iwanaga, S., Shimeno, S., 1994. Effects of dietary glucose, fructose, andgalactose on hepatopancreatic enzyme activities and body composition in carp. Fish. Sci.60, 613\u2013617. https:\/\/doi.org\/10.2331\/fishsci.60.613","DOI":"10.2331\/fishsci.60.613"},{"key":"1671_CR29","doi-asserted-by":"publisher","DOI":"10.1590\/0001-3765202120201541","volume":"93","author":"AM Souza","year":"2021","unstructured":"Souza AM, Copatti CE, Campeche DFB, Melo FVST, Melo JFB (2021) Glucose tolerance in six fish species reared in Brazil: differences between carnivorous and omnivorous. An Acad Bras Cienc 93:e20201541. https:\/\/doi.org\/10.1590\/0001-3765202120201541","journal-title":"An Acad Bras Cienc"},{"key":"1671_CR31","doi-asserted-by":"publisher","first-page":"1182","DOI":"10.1111\/are.13571","volume":"49","author":"LS Takahashi","year":"2018","unstructured":"Takahashi LS, Ha N, Pereira MM, Biller-Takahashi JD, Urbinati EC (2018) Carbohydrate tolerance in the fruit-eating fish Piaractus mesopotamicus (Holmberg, 1887). Aquac Res 49:1182\u20131188. https:\/\/doi.org\/10.1111\/are.13571","journal-title":"Aquac Res"},{"key":"1671_CR32","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12111142","volume":"12","author":"I Viegas","year":"2022","unstructured":"Viegas I, Di Nunzio G, Belew GD, Torres AN, Silva JG, Perp\u00e9tuo L, Barosa C, Tavares LC, Jones JG (2022) Integration of liver glycogen and triglyceride NMR isotopomer analyses provides a comprehensive coverage of hepatic glucose and fructose metabolism. Metabolites 12:1142. https:\/\/doi.org\/10.3390\/metabo12111142","journal-title":"Metabolites"},{"key":"1671_CR33","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/0044-8486(90)90162-G","volume":"88","author":"MM Vijayan","year":"1990","unstructured":"Vijayan MM, Ballantyne JS, Leatherland JF (1990) High stocking density alters the energy metabolism of brook charr, Salvelinus fontinalis. Aquaculture 88:371\u2013381. https:\/\/doi.org\/10.1016\/0044-8486(90)90162-G","journal-title":"Aquaculture"}],"container-title":["Journal of Comparative Physiology B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00360-026-01671-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00360-026-01671-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00360-026-01671-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:03:47Z","timestamp":1787681027000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00360-026-01671-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,27]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["1671"],"URL":"https:\/\/doi.org\/10.1007\/s00360-026-01671-2","relation":{},"ISSN":["0174-1578","1432-136X"],"issn-type":[{"value":"0174-1578","type":"print"},{"value":"1432-136X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,27]]},"assertion":[{"value":"25 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 February 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 May 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 June 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experimental procedures in this study were approved by the Brazilian College of Animal Experimentation (COBEA) and Ethics Committee on Animal Use (CEUA), Sao Paulo State University (UNESP), protocol n\u00b0 07\/2024. R1\u2010CEUA.","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}]}}