{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:26:14Z","timestamp":1787682374545,"version":"build-2784847793"},"reference-count":73,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade Federal De S\u00e3o Carlos"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Comp Physiol B"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Estuarine fishes experience marked environmental variability and rely on tightly regulated osmoregulatory mechanisms to maintain internal homeostasis. Settleable atmospheric particulate matter (SePM), a complex mixture of metals and metalloids, is an emerging stressor in aquatic systems and may interfere with regulatory processes. This study investigated the effects of SePM exposure on osmoregulatory homeostasis in the estuarine fish\n                    <jats:italic>Centropomus parallelus<\/jats:italic>\n                    . Fish were exposed for 96\u00a0h to either 0\u00a0g.L\u207b\u00b9 (control) or 1\u00a0g.L\u207b\u00b9 SePM, and key physiological endpoints associated with ion and water balance were assessed, including plasma osmolality and Na\u207a, Cl\u207b, and K\u207a concentrations; Na\u207a\/K\u207a-ATPase (NKA), V-type H\n                    <jats:sup>+<\/jats:sup>\n                    -ATPase (VAT), and total ATPase activities in the gills and kidneys; and ionocyte abundance and transporter protein levels in gill ionocytes and renal tubules. Aluminum, iron, vanadium, and chromium levels were accumulated in the gills and kidneys. SePM exposure disrupted osmoregulatory homeostasis, as evidenced by reduced plasma osmolality and decreased Cl\n                    <jats:sup>\u2013<\/jats:sup>\n                    and K\n                    <jats:sup>+<\/jats:sup>\n                    concentrations. NKA activity was upregulated in the gills, accompanied by an increase in the density of NKA-positive ionocytes. Conversely, total ATPase and VAT activities were downregulated in the kidneys, whereas the abundance of NKA, the symporter Na\n                    <jats:sup>+<\/jats:sup>\n                    ,K\n                    <jats:sup>+<\/jats:sup>\n                    ,2Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    (NKCC), and the cystic fibrosis transmembrane conductance regulator (CFTR), a chloride (Cl\u207b) channel, remained unchanged in renal tubules. These findings suggest that SePM exposure is associated with tissue-specific modulation of osmoregulatory parameters, including increased branchial Na\u207a\/K\u207a-ATPase activity and NKA-positive ionocyte density, and reduced renal ATPase activities, accompanied by alterations in plasma osmolality and ion concentrations. The physiological disturbances associated with SePM exposure may impair organismal performance and reduce ecological fitness in estuarine fishes inhabiting metal-impacted coastal environments.\n                  <\/jats:p>","DOI":"10.1007\/s00360-026-01698-5","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T11:52:57Z","timestamp":1785153177000},"page":"535-551","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Osmoregulatory disruption and metal bioaccumulation in the estuarine fish Centropomus parallelus exposed to settleable atmospheric particulate matter"],"prefix":"10.1007","volume":"196","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8653-9709","authenticated-orcid":false,"given":"Anieli Cristina","family":"Maraschi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Aurelio Miranda","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane Gomes","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iara Costa","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena Victoria","family":"Monferran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Alberto","family":"Wunderlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiano","family":"Bendhack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Amaral","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa Narciso","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"1698_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2022.159168","volume":"856","author":"HA Adorno","year":"2023","unstructured":"Adorno HA, Souza IC, Monferr\u00e1n MV, Wunderlin DA, Fernandes MN, Monteiro DA (2023) A multi-biomarker approach to assess the sublethal effects of settleable atmospheric particulate matter from an industrial area on Nile tilapia (Oreochromis niloticus). Sci Total Environ 856:159168. https:\/\/doi.org\/10.1016\/j.scitotenv.2022.159168","journal-title":"Sci Total Environ"},{"issue":"2","key":"1698_CR2","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1016\/j.cbpc.2006.12.012","volume":"145","author":"G Atli","year":"2007","unstructured":"Atli G, Canli M (2007) Enzymatic responses to metal exposures in a freshwater fish Oreochromis niloticus. Comp Biochem Physiol C Toxicol Pharmacol 145(2):282\u2013287. https:\/\/doi.org\/10.1016\/j.cbpc.2006.12.012","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"issue":"12","key":"1698_CR3","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1002\/tox.21893","volume":"28","author":"G Atli","year":"2013","unstructured":"Atli G, Canli M (2013) Metals (Ag+, Cd2+, Cr6+) affect ATPase activity in the gill, kidney, and muscle of freshwater fish Oreochromis niloticus following acute and chronic exposures. Environ Toxicol 28(12):707\u2013717. https:\/\/doi.org\/10.1002\/tox.21893","journal-title":"Environ Toxicol"},{"key":"1698_CR4","volume-title":"Theory and practice of histological techniques","year":"2008","unstructured":"Bancroft JD, Gamble M (eds) (2008) Theory and practice of histological techniques, 6th edn. Elsevier Health Sciences, Philadelphia","edition":"6th edn"},{"issue":"1\u20132","key":"1698_CR5","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1\u20132):248\u2013254. https:\/\/doi.org\/10.1016\/0003-2697(76)90527-3","journal-title":"Anal Biochem"},{"issue":"9","key":"1698_CR6","doi-asserted-by":"publisher","first-page":"e04691","DOI":"10.1016\/j.heliyon.2020.e04691","volume":"6","author":"J Briffa","year":"2020","unstructured":"Briffa J, Sinagra E, Blundell R (2020) Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon 6(9):e04691. https:\/\/doi.org\/10.1016\/j.heliyon.2020.e04691","journal-title":"Heliyon"},{"issue":"1","key":"1698_CR7","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.aquatox.2009.05.017","volume":"94","author":"MMP Camargo","year":"2009","unstructured":"Camargo MMP, Fernandes MN, Martinez CBR (2009) How aluminium exposure promotes osmoregulatory disturbances in the neotropical freshwater fish Prochilus lineatus. Aquat Toxicol 94(1):40\u201346. https:\/\/doi.org\/10.1016\/j.aquatox.2009.05.017","journal-title":"Aquat Toxicol"},{"issue":"23","key":"1698_CR8","doi-asserted-by":"publisher","first-page":"22963","DOI":"10.1007\/s11356-018-2393-4","volume":"25","author":"TLL Carmo","year":"2018","unstructured":"Carmo TLL, Azevedo VC, de Siqueira PR, Galv\u00e3o TD, dos Santos FA, dos Reis Martinez CB, Appoloni CR, Fernandes MN (2018) Reactive oxygen species and other biochemical and morphological biomarkers in the gills and kidneys of the Neotropical freshwater fish, Prochilodus lineatus, exposed to titanium dioxide (TiO2) nanoparticles. Environ Sci Pollut Res 25(23):22963\u201322976. https:\/\/doi.org\/10.1007\/s11356-018-2393-4","journal-title":"Environ Sci Pollut Res"},{"issue":"4","key":"1698_CR9","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1002\/tox.22699","volume":"34","author":"TLL Carmo","year":"2019","unstructured":"Carmo TLL, Siqueira PR, Azevedo VC, Tavares D, Pesenti EC, Cestari MM, Martinez CBR, Fernandes MN (2019) Overview of the toxic effects of titanium dioxide nanoparticles in blood, liver, muscles, and brain of a Neotropical detritivorous fish. Environ Toxicol 34(4):457\u2013468. https:\/\/doi.org\/10.1002\/tox.22699","journal-title":"Environ Toxicol"},{"key":"1698_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.114159","author":"T Chandrapalan","year":"2020","unstructured":"Chandrapalan T, Kwong RWM (2020) Influence of dietary iron exposure on trace metal homeostasis and expression of metal transporters during development in zebrafish. Environ Pollut. https:\/\/doi.org\/10.1016\/j.envpol.2020.114159","journal-title":"Environ Pollut"},{"key":"1698_CR11","doi-asserted-by":"publisher","first-page":"122846","DOI":"10.1016\/j.envpol.2023.122846","volume":"340","author":"RL Costa","year":"2024","unstructured":"Costa RL, Souza IC, Morozesk M, de Carvalho LB, Carvalho CS, Monferr\u00e1n MV, Wunderlin DA, Fernandes MN, Monteiro DA (2024) Toxic, genotoxic, mutagenic, and bioaccumulative effects of metal mixture from settleable particulate matter on American bullfrog tadpoles (Lithobates catesbeianus). Environ Pollut 340:122846. https:\/\/doi.org\/10.1016\/j.envpol.2023.122846","journal-title":"Environ Pollut"},{"key":"1698_CR12","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1002\/cphy.c170018","volume":"8","author":"E Delpire","year":"2018","unstructured":"Delpire E, Gagnon KB (2018) Na+-K+-2Cl\u2212 Cotransporter (NKCC) Physiological Function in Nonpolarized Cells and Transporting Epithelia. Compr Physiol 8:871\u2013901. https:\/\/doi.org\/10.1002\/cphy.c170018","journal-title":"Compr Physiol"},{"issue":"6","key":"1698_CR13","doi-asserted-by":"publisher","DOI":"10.3390\/biology14060667","volume":"14","author":"T Dildar","year":"2025","unstructured":"Dildar T, Cui W, Ikhwanuddin M, Ma H (2025) Aquatic organisms in response to salinity stress: ecological impacts, adaptive mechanisms, and resilience strategies. Biology (Basel) 14(6):667. https:\/\/doi.org\/10.3390\/biology14060667","journal-title":"Biology (Basel)"},{"key":"1698_CR14","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1016\/j.cbpa.2013.07.022","volume":"166","author":"RM Duarte","year":"2013","unstructured":"Duarte RM, Ferreira MS, Wood CM, Val AL (2013) Effect of low pH exposure on Na+ regulation in two cichlid fish species of the Amazon. Comp Biochem Physiol A Mol Integr Physiol 166:441\u2013448. https:\/\/doi.org\/10.1016\/j.cbpa.2013.07.022","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"key":"1698_CR15","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1023\/A:1023295413119","volume":"25","author":"B Dussault","year":"2001","unstructured":"Dussault B, Playle RC, Dixon DG, McKinley RS (2001) Effects of sublethal, acidic aluminum exposure on blood ions and metabolites, cardiac output, heart rate, and stroke volume of rainbow trout, Oncorhynchus mykiss. Fish Physiol Biochem 25:197\u2013206. https:\/\/doi.org\/10.1023\/A:1023295413119","journal-title":"Fish Physiol Biochem"},{"key":"1698_CR16","unstructured":"Eisler R (1989) Molybdenum hazards to fish, wildlife, and invertebrates: a synoptic review (No. 19). Fish and Wildlife Service, US Department of the Interior."},{"key":"1698_CR17","unstructured":"EPA (1994) Method 200.8, Revision 5.4. Determination of Trace Elements in Waters and Wastes by Inductively Coupled Plasma \u2013 Mass Spectrometry. US Environmental Protection Agency. https:\/\/www.epa.gov\/sites\/default\/files\/2015-06\/documents\/method_200-8_rev_5-4_1994.pdf"},{"issue":"1","key":"1698_CR18","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1152\/physrev.00050.2003","volume":"85","author":"DH Evans","year":"2005","unstructured":"Evans DH, Piermarini PM, Choe KP (2005) The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85(1):97\u2013177. https:\/\/doi.org\/10.1152\/physrev.00050.2003","journal-title":"Physiol Rev"},{"key":"1698_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2024.141576","author":"IF Fernandes","year":"2024","unstructured":"Fernandes IF, Fujiwara GH, Moraes Utsunomiya HS, Souza IC, Monteiro DA, Monferr\u00e1n MV, Wunderlin DA, Fernandes MN, Carvalho CS (2024) Oxidative stress and neurotoxicity induced by exposure to settleable atmospheric particulate matter in bullfrog tadpoles, Aquarana catesbeiana. Chemosphere. https:\/\/doi.org\/10.1016\/j.chemosphere.2024.141576","journal-title":"Chemosphere"},{"key":"1698_CR20","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.166119","author":"WMPA Fortes","year":"2023","unstructured":"Fortes WMPA, Souza IC, Azevedo VC, Griboff J, Monferr\u00e1n MV, Wunderlin DA, Matsumoto ST, Fernandes MN (2023) Metal\/metalloid bioconcentration dynamics in fish and the risk to human health due to water contamination with atmospheric particulate matter from a metallurgical industrial area. Sci Total Environ. https:\/\/doi.org\/10.1016\/j.scitotenv.2023.166119","journal-title":"Sci Total Environ"},{"key":"1698_CR72","doi-asserted-by":"publisher","unstructured":"Gantayat RR, Elumalai V (2024) Salinity-induced changes in heavy metal behavior and mobility in semi-arid coastal aquifers: a comprehensive review. Water, 16(7), 1052. https:\/\/doi.org\/10.3390\/w16071052","DOI":"10.3390\/w16071052"},{"issue":"1","key":"1698_CR21","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1242\/jeb.143.1.475","volume":"143","author":"A Gibbs","year":"1989","unstructured":"Gibbs A, Somero GN (1989) Pressure adaptation of Na+\/K+-ATPase in gills of marine teleosts. J Exp Biol 143(1):475\u2013492","journal-title":"J Exp Biol"},{"issue":"3","key":"1698_CR22","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1007\/s00360-011-0624-9","volume":"182","author":"RJ Gonzalez","year":"2012","unstructured":"Gonzalez RJ (2012) The physiology of hyper-salinity tolerance in teleost fish: a review. J Comp Physiol B 182(3):321\u2013329. https:\/\/doi.org\/10.1007\/s00360-011-0624-9","journal-title":"J Comp Physiol B"},{"issue":"16","key":"1698_CR23","doi-asserted-by":"publisher","first-page":"3148","DOI":"10.1242\/jeb.083550","volume":"216","author":"C Grassie","year":"2013","unstructured":"Grassie C, Braithwaite VA, Nilsson J, Nilsen TO, Teien HC, Handeland SO, Ebbesson LOE (2013) Aluminum exposure impacts brain plasticity and behavior in Atlantic salmon (Salmo salar). J Exp Biol 216(16):3148\u20133155. https:\/\/doi.org\/10.1242\/jeb.083550","journal-title":"J Exp Biol"},{"issue":"8","key":"1698_CR25","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1242\/jeb.205.8.1179","volume":"205","author":"M Grosell","year":"2002","unstructured":"Grosell M, Wood CM (2002) Copper uptake across rainbow trout gills: mechanisms of apical entry. J Exp Biol 205(8):1179\u20131188. https:\/\/doi.org\/10.1242\/jeb.205.8.1179","journal-title":"J Exp Biol"},{"issue":"1\u20132","key":"1698_CR24","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/S1532-0456(02)00085-6","volume":"133","author":"M Grosell","year":"2002","unstructured":"Grosell M, Nielsen C, Bianchini A (2002) Sodium turnover rate determines sensitivity to acute copper and silver exposure in freshwater animals. Comp Biochem Physiol C Toxicol Pharmacol 133(1\u20132):287\u2013303. https:\/\/doi.org\/10.1016\/S1532-0456(02)00085-6","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"key":"1698_CR26","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2019.103536","volume":"104","author":"B Hernroth","year":"2020","unstructured":"Hernroth B, Tassidis H, Baden SP (2020) Immunosuppression of aquatic organisms exposed to elevated levels of manganese: from global to molecular perspective. Dev Comp Immunol 104:103536. https:\/\/doi.org\/10.1016\/j.dci.2019.103536","journal-title":"Dev Comp Immunol"},{"issue":"3","key":"1698_CR27","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.resp.2012.07.019","volume":"184","author":"J Hiroi","year":"2012","unstructured":"Hiroi J, McCormick SD (2012) New insights into gill ionocyte and ion transporter function in euryhaline and diadromous fish. Respir Physiol Neurobiol 184(3):257\u2013268. https:\/\/doi.org\/10.1016\/j.resp.2012.07.019","journal-title":"Respir Physiol Neurobiol"},{"key":"1698_CR28","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2023.106592","author":"JL Horng","year":"2023","unstructured":"Horng JL, Lee YS, Lin LY (2023) Exposure to silver impairs the osmoregulatory capability of euryhaline medaka (Oryzias latipes) subjected to salinity changes. Aquat Toxicol. https:\/\/doi.org\/10.1016\/j.aquatox.2023.106592","journal-title":"Aquat Toxicol"},{"issue":"1","key":"1698_CR29","doi-asserted-by":"publisher","first-page":"R28","DOI":"10.1152\/ajpregu.00047.2011","volume":"301","author":"PP Hwang","year":"2011","unstructured":"Hwang PP, Lee TH, Lin LY (2011) Ion regulation in fish gills: recent progress in the cellular and molecular mechanisms. Am J Physiol Regul Integr Comp Physiol 301(1):R28\u2013R47. https:\/\/doi.org\/10.1152\/ajpregu.00047.2011","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"1698_CR30","unstructured":"IEMA (2021) Instituto de Meio Ambiente e Recursos H\u00eddricos. https:\/\/iema.es.gov.br\/"},{"key":"1698_CR31","doi-asserted-by":"publisher","first-page":"1883","DOI":"10.1242\/jeb.198.9.1883","volume":"198","author":"D Kultz","year":"1995","unstructured":"Kultz D, Somero GN (1995) Osmotic and thermal effects on in situ ATPase activity in permeabilized gill epithelial cells of the fish Gillichthys mirabilis. J Exp Biol 198:1883\u20131894. https:\/\/doi.org\/10.1242\/jeb.198.9.1883","journal-title":"J Exp Biol"},{"issue":"1","key":"1698_CR32","doi-asserted-by":"publisher","first-page":"8505","DOI":"10.1038\/s41598-023-35787-0","volume":"13","author":"N Kumar","year":"2023","unstructured":"Kumar N, Thorat ST, Reddy KS (2023) Multi biomarker approach to assess manganese and manganese nanoparticles toxicity in Pangasianodon hypophthalmus. Sci Rep 13(1):8505. https:\/\/doi.org\/10.1038\/s41598-023-35787-0","journal-title":"Sci Rep"},{"issue":"5","key":"1698_CR33","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1007\/s00360-024-01565-1","volume":"194","author":"RWM Kwong","year":"2024","unstructured":"Kwong RWM (2024) Trace metals in the teleost fish gill: biological roles, uptake regulation, and detoxification mechanisms. J Comp Physiol B 194(5):749\u2013763. https:\/\/doi.org\/10.1007\/s00360-024-01565-1","journal-title":"J Comp Physiol B"},{"issue":"1","key":"1698_CR34","doi-asserted-by":"publisher","first-page":"H1","DOI":"10.1152\/ajpheart.00446.2025","volume":"330","author":"TTN Le","year":"2026","unstructured":"Le TTN, Sepp\u00e4nen E, Haverinen J, Anttila K (2026) Modifying the electrical excitability of cardiomyocytes in rainbow trout (Oncorhynchus mykiss) through exercise training. Am J Physiol Heart Circ Physiol 330(1):H1\u2013H11. https:\/\/doi.org\/10.1152\/ajpheart.00446.2025","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"1698_CR35","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2023.109757","author":"FA Leone","year":"2023","unstructured":"Leone FA, Fabri LM, Costa MIC, Moraes CM, Gar\u00e7on DP, McNamara JC (2023) Differential effects of cobalt ions in vitro on gill (Na+, K+)-ATPase kinetics in the Blue crab Callinectes danae (Decapoda, Brachyura). Comp Biochem Physiol C Toxicol Pharmacol. https:\/\/doi.org\/10.1016\/j.cbpc.2023.109757","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"key":"1698_CR36","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2022.153862","volume":"825","author":"Q Li","year":"2022","unstructured":"Li Q, Wang Y, Li Y, Li L, Tang M, Hu W, Chen L, Ai S (2022) Speciation of heavy metals in soils and their immobilization at micro-scale interfaces among diverse soil components. Sci Total Environ 825:153862. https:\/\/doi.org\/10.1016\/j.scitotenv.2022.153862","journal-title":"Sci Total Environ"},{"key":"1698_CR73","doi-asserted-by":"publisher","unstructured":"Luoma SN, Rainbow PS (2005) Why is metal bioaccumulation so variable? Biodynamics as a unifying concept. Environ Sci Technol 39(7):1921\u201331. https:\/\/doi.org\/10.1021\/es048947e","DOI":"10.1021\/es048947e"},{"key":"1698_CR40","doi-asserted-by":"publisher","first-page":"135316","DOI":"10.1016\/j.jhazmat.2024.135316","volume":"477","author":"AC Maraschi","year":"2024","unstructured":"Maraschi AC, Rubio-Lopez C, Snitman SM, Souza IC, Pichardo-Casales B, Alcaraz G, Monferr\u00e1n MV, Wunderlin DA, Caamal-Monsreal C, Rosas C, Fernandes MN, Capparelli MV (2024c) The impact of settleable atmospheric particulate on the energy metabolism, biochemical processes, and behavior of a sentinel mangrove crab. J Hazard Mater 477:135316. https:\/\/doi.org\/10.1016\/j.jhazmat.2024.135316","journal-title":"J Hazard Mater"},{"key":"1698_CR37","doi-asserted-by":"publisher","first-page":"177423","DOI":"10.1016\/j.scitotenv.2024.177423","volume":"956","author":"AC Maraschi","year":"2024","unstructured":"Maraschi AC, Adorno HA, Gon\u00e7alves YC, Souza IC, Monferr\u00e1n MV, Wunderlin DA, Fernandes MN, Monteiro DA (2024b) Effects of metallic dust on Nile tilapia: Exploring the relationship between metal bioaccumulation, metallothionein levels, and oxidative stress responses. Sci Total Environ 956:177423. https:\/\/doi.org\/10.1016\/j.scitotenv.2024.177423","journal-title":"Sci Total Environ"},{"issue":"6","key":"1698_CR39","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1007\/s10641-024-01556-9","volume":"107","author":"AC Maraschi","year":"2024","unstructured":"Maraschi AC, Gutierre SM, Prodocimo V (2024a) Osmoregulation and HSP70 abundance in freshwater catfish Rhamdia quelen under acute salt exposure. Environ Biol Fishes 107(6):631\u2013644. https:\/\/doi.org\/10.1007\/s10641-024-01556-9","journal-title":"Environ Biol Fishes"},{"issue":"6","key":"1698_CR38","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1007\/s00128-025-04151-5","volume":"115","author":"AC Maraschi","year":"2025","unstructured":"Maraschi AC, Moro IC, Dos Santos VG, Souza IC, Monferran MV, Wunderlin DA, Bendhack F, Fernandes MN, Monteiro DA (2025) Air-Water Cross-Contamination by Metalliferous Atmospheric Particulates: Bioaccumulation and Hematological Impairments in the Estuarine Fish Centropomus parallelus. Bull Environ Contam Toxicol 115(6):76. https:\/\/doi.org\/10.1007\/s00128-025-04151-5","journal-title":"Bull Environ Contam Toxicol"},{"issue":"3","key":"1698_CR42","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1111\/j.1748-1716.2010.02189.x","volume":"202","author":"WS Marshall","year":"2011","unstructured":"Marshall WS (2011) Mechanosensitive signalling in fish gill and other ion transporting epithelia. Acta Physiol 202(3):487\u2013499. https:\/\/doi.org\/10.1111\/j.1748-1716.2010.02189.x","journal-title":"Acta Physiol"},{"key":"1698_CR41","first-page":"pp 177","volume-title":"Physiology of Fishes","author":"WS Marshall","year":"2005","unstructured":"Marshall WS, Grosell M (2005) Ion transport, osmoregulation and acid-base balance. In: Evans D, Claiborne JB (eds) Physiology of Fishes, vol vol 3. CRC Press, Boca Raton, FL, p pp 177-230"},{"key":"1698_CR43","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.atmosres.2016.08.014","volume":"183","author":"S Mbengue","year":"2017","unstructured":"Mbengue S, Alleman LY, Flament P (2017) Metal-bearing fine particle sources in a coastal industrialized environment. Atmospheric Research 183:202\u2013211. https:\/\/doi.org\/10.1016\/j.atmosres.2016.08.014","journal-title":"Atmospheric Research"},{"key":"1698_CR44","first-page":"pp 247","volume-title":"Fish ecophysiology","author":"SD McCormick","year":"1993","unstructured":"McCormick SD (1993) Methods for nonlethal gill biopsy and measurement of Na\u207a\/K\u207a-ATPase activity. In: Rankin JC, Jensen FB (eds) Fish ecophysiology. Chapman & Hall, London, p pp 247-254"},{"key":"1698_CR45","first-page":"285","volume-title":"Fish physiology","author":"SD McCormick","year":"1995","unstructured":"McCormick SD (1995) Hormonal control of gill Na\u207a\/K\u207a-ATPase and chloride cell function. In: Wood CM, Shuttleworth TJ (eds) Fish physiology, vol Vol 14. Academic Press, San Diego, pp 285\u2013315"},{"key":"1698_CR46","doi-asserted-by":"publisher","unstructured":"McDonald DG, Wood CM (1993) Branchial mechanisms of acclimation to metals in freshwater fish. In: Rankin JC, Jensen FB (eds) Fish Ecophysiology. Chapman & Hall Fish and Fisheries Series, vol 9. Springer, Dordrecht, pp 297\u2013321. https:\/\/doi.org\/10.1007\/978-94-011-2304-4_12","DOI":"10.1007\/978-94-011-2304-4_12"},{"issue":"3","key":"1698_CR47","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/S0166-445X(99)00105-8","volume":"50","author":"JC McGeer","year":"2000","unstructured":"McGeer JC, Szebedinszky C, McDonald DG, Wood CM (2000) Effects of chronic sublethal exposure to waterborne Cu, Cd or Zn in rainbow trout. 1: Iono-regulatory disturbance and metabolic costs. Aquat Toxicol 50(3):231\u2013243. https:\/\/doi.org\/10.1016\/S0166-445X(99)00105-8","journal-title":"Aquat Toxicol"},{"key":"1698_CR48","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.atmosenv.2013.11.011","volume":"83","author":"K Mohiuddin","year":"2014","unstructured":"Mohiuddin K, Strezov V, Nelson PF, Stelcer E (2014) Characterisation of trace metals in atmospheric particles in the vicinity of iron and steelmaking industries in Australia. Atmospheric Environ 83:72\u201379. https:\/\/doi.org\/10.1016\/j.atmosenv.2013.11.011","journal-title":"Atmospheric Environ"},{"key":"1698_CR49","doi-asserted-by":"publisher","first-page":"138715","DOI":"10.1016\/j.chemosphere.2023.138715","volume":"330","author":"R Monteiro","year":"2023","unstructured":"Monteiro R, Souza IC, Morozesk M, Soares MP, De Angelis CF, Vieira NS, Bendhack F, Monferr\u00e1n MV, Wunderlin DA, Fernandes MN (2023) Metalliferous atmospheric settleable particulate matter action on the fat snook fish (Centropomus parallelus): Metal bioaccumulation, antioxidant responses and histological changes in gills, hepatopancreas and kidneys. Chemosphere 330:138715. https:\/\/doi.org\/10.1016\/j.chemosphere.2023.138715","journal-title":"Chemosphere"},{"key":"1698_CR50","doi-asserted-by":"publisher","first-page":"129751","DOI":"10.1016\/j.chemosphere.2021.129751","volume":"274","author":"L Negral","year":"2021","unstructured":"Negral L, Su\u00e1rez-Pe\u00f1a B, Amado \u00c1, Megido L, Lara R, Mara\u00f1\u00f3n E, Castrill\u00f3n L (2021) Settleable matter in a highly industrialized area: Chemistry and health risk assessment. Chemosphere 274:129751. https:\/\/doi.org\/10.1016\/j.chemosphere.2021.129751","journal-title":"Chemosphere"},{"key":"1698_CR51","doi-asserted-by":"publisher","first-page":"173295","DOI":"10.1016\/j.scitotenv.2024.173295","volume":"938","author":"CR Nobre","year":"2024","unstructured":"Nobre CR, de Souza Pa\u00e7o M, de Almeida Duarte LF et al (2024) Systemic effects of settleable atmospheric particulate matter (SePM) on swamp ghost crab Ucides cordatus. Sci Total Environ 938:173295. https:\/\/doi.org\/10.1016\/j.scitotenv.2024.173295","journal-title":"Sci Total Environ"},{"key":"1698_CR52","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2020.108705","volume":"230","author":"LS Nogueira","year":"2020","unstructured":"Nogueira LS, Chen CC, Wood CM, Kelly SP (2020) Effects of copper on a reconstructed freshwater rainbow trout gill epithelium: paracellular and intracellular aspects. Comp Biochem Physiol C Toxicol Pharmacol 230:108705. https:\/\/doi.org\/10.1016\/j.cbpc.2020.108705","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"key":"1698_CR53","doi-asserted-by":"publisher","DOI":"10.1787\/9789264069961-en","volume-title":"OECD Guidelines for the Testing of Chemicals, Section 2","author":"OECD","year":"2019","unstructured":"OECD (2019) Test no. 203: fish, acute toxicity test. OECD Guidelines for the Testing of Chemicals, Section 2. OECD Publishing, Paris. https:\/\/doi.org\/10.1787\/9789264069961-en"},{"key":"1698_CR54","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1007\/BF00694378","volume":"159","author":"RC Playle","year":"1989","unstructured":"Playle RC, Wood CM (1989) Water pH and aluminum chemistry in the gill micro-environment of rainbow trout during acid and aluminum exposures. J Comp Physiol B 159:539\u2013550. https:\/\/doi.org\/10.1007\/BF00694378","journal-title":"J Comp Physiol B"},{"key":"1698_CR55","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2020.106284","author":"HM Queiroz","year":"2021","unstructured":"Queiroz HM, Ying SC, Abernathy M, Barcellos D, Gabriel FA, Otero XL, N\u00f3brega GN, Bernardino AF, Ferreira TO (2021) Manganese: The overlooked contaminant in the world largest mine tailings dam collapse. Environ Int. https:\/\/doi.org\/10.1016\/j.envint.2020.106284","journal-title":"Environ Int"},{"key":"1698_CR56","unstructured":"R Core Team (2024) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. https:\/\/www.R-project.org\/"},{"issue":"5","key":"1698_CR57","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1039\/C4MT00304G","volume":"7","author":"D Rehder","year":"2015","unstructured":"Rehder D (2015) The role of vanadium in biology. Metallomics 7(5):730\u2013742","journal-title":"Metallomics"},{"key":"1698_CR58","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.ecoenv.2013.02.020","volume":"92","author":"D Saglam","year":"2013","unstructured":"Saglam D, Atli G, Canli M (2013) Investigations on the osmoregulation of freshwater fish (Oreochromis niloticus) following exposures to metals (Cd, Cu) in differing hardness. Ecotoxicol Environ Saf 92:79\u201386. https:\/\/doi.org\/10.1016\/j.ecoenv.2013.02.020","journal-title":"Ecotoxicol Environ Saf"},{"issue":"27","key":"1698_CR59","doi-asserted-by":"publisher","first-page":"22026","DOI":"10.1007\/s11356-017-9677-y","volume":"24","author":"JM Santos","year":"2017","unstructured":"Santos JM, Reis NC, Galv\u00e3o ES, Silveira A, Goulart EV, Lima AT (2017) Source apportionment of settleable particles in an impacted urban and industrialized region in Brazil. Environ Sci Pollut Res 24(27):22026\u201322039. https:\/\/doi.org\/10.1007\/s11356-017-9677-y","journal-title":"Environ Sci Pollut Res"},{"key":"1698_CR60","doi-asserted-by":"publisher","first-page":"125900","DOI":"10.1016\/j.chemosphere.2020.125900","volume":"247","author":"MH Sayadi","year":"2020","unstructured":"Sayadi MH, Mansouri B, Shahri E, Tyler CR, Shekari H, Kharkan J (2020) Exposure effects of iron oxide nanoparticles and iron salts in blackfish (Capoeta fusca): Acute toxicity, bioaccumulation, depuration, and tissue histopathology. Chemosphere 247:125900. https:\/\/doi.org\/10.1016\/j.chemosphere.2020.125900","journal-title":"Chemosphere"},{"key":"1698_CR61","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671\u2013675. https:\/\/doi.org\/10.1038\/nmeth.2089","journal-title":"Nat Methods"},{"issue":"7","key":"1698_CR62","doi-asserted-by":"publisher","first-page":"6597","DOI":"10.3390\/ijms24076597","volume":"24","author":"SW Shih","year":"2023","unstructured":"Shih SW, Yan JJ, Lu SW, Chuang YT, Lin HW, Chou MY, Hwang PP (2023) Molecular physiological evidence for the role of Na+-Cl \u2212 Co-Transporter in branchial Na+ uptake in freshwater teleosts. Int J Mol Sci 24(7):6597. https:\/\/doi.org\/10.3390\/ijms24076597","journal-title":"Int J Mol Sci"},{"key":"1698_CR63","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.envpol.2013.05.017","volume":"180","author":"IC Souza","year":"2013","unstructured":"Souza IC, Duarte ID, Pimentel NQ, Rocha LD, Morozesk M, Bonomo MM, Fernandes MN (2013) Matching metal pollution with bioavailability, bioaccumulation and biomarkers response in fish (Centropomus parallelus) resident in neotropical estuaries. Environ Pollut 180:136\u2013144. https:\/\/doi.org\/10.1016\/j.envpol.2013.05.017","journal-title":"Environ Pollut"},{"key":"1698_CR65","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.144651","volume":"770","author":"IC Souza","year":"2021","unstructured":"Souza IC, Arrivabene HP, Azevedo VC, Duarte ID, Rocha LD, Matsumoto ST, Franco A, Elliott M, Wunderlin DA, Monferr\u00e1n MV, Fernandes MN (2021b) Different trophodynamics between two proximate estuaries with differing degrees of pollution. Sci Total Environ 770:144651. https:\/\/doi.org\/10.1016\/j.scitotenv.2020.144651","journal-title":"Sci Total Environ"},{"key":"1698_CR64","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.141976","volume":"753","author":"IC Souza","year":"2021","unstructured":"Souza IC, Morozesk M, Mansano AS, Mendes VAS, Azevedo VC, Matsumoto ST, Elliott M, Monferr\u00e1n MV, Wunderlin DA, Fernandes MN (2021a) Atmospheric particulate matter from an industrial area as a source of metal nanoparticle contamination in aquatic ecosystems. Sci Total Environ 753:141976. https:\/\/doi.org\/10.1016\/j.scitotenv.2020.141976","journal-title":"Sci Total Environ"},{"issue":"15","key":"1698_CR66","doi-asserted-by":"publisher","first-page":"8869","DOI":"10.1021\/es401380p","volume":"47","author":"TM Uren Webster","year":"2013","unstructured":"Uren Webster TM, Bury N, van Aerle R, Santos EM (2013) Global transcriptome profiling reveals molecular mechanisms of metal tolerance in a chronically exposed wild population of brown trout. Environ Sci Technol 47(15):8869\u20138877. https:\/\/doi.org\/10.1021\/es401380p","journal-title":"Environ Sci Technol"},{"issue":"2","key":"1698_CR67","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1515\/reveh.2009.24.2.129","volume":"24","author":"V Velma","year":"2009","unstructured":"Velma V, Vutukuru SS, Tchounwou PB (2009) Ecotoxicology of hexavalent chromium in freshwater fish: a critical review. Reviews on environmental health 24(2):129. https:\/\/doi.org\/10.1515\/reveh.2009.24.2.129","journal-title":"Reviews on environmental health"},{"key":"1698_CR69","doi-asserted-by":"publisher","unstructured":"Wood CM (2011). An introduction to metals in fish physiology and toxicology: basic principles. In: Wood, C.M., Farrell, A.P., Brauner, C.J. (eds) Fish Physiology, Vol. 31. Academic Press. https:\/\/doi.org\/10.1016\/S1546-5098(11)31001-1","DOI":"10.1016\/S1546-5098(11)31001-1"},{"key":"1698_CR68","doi-asserted-by":"publisher","unstructured":"Wood CM (1989). The physiological problems of fish in acid waters. In: Morris, R. et al. (eds) Acid Toxicity and Aquatic Animals. Cambridge University Press. https:\/\/doi.org\/10.1017\/CBO9780511983344.010","DOI":"10.1017\/CBO9780511983344.010"},{"issue":"2","key":"1698_CR70","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.aquatox.2010.04.012","volume":"99","author":"CM Wood","year":"2010","unstructured":"Wood CM, Grosell M, McDonald MD, Playle RC, Walsh PJ (2010) Effects of waterborne silver in a marine teleost, the gulf toadfish (Opsanus beta): Effects of feeding and chronic exposure on bioaccumulation and physiological responses. Aquat Toxicol 99(2):138\u2013148. https:\/\/doi.org\/10.1016\/j.aquatox.2010.04.012","journal-title":"Aquat Toxicol"},{"issue":"1","key":"1698_CR71","doi-asserted-by":"publisher","first-page":"7654","DOI":"10.1038\/s41598-020-64519-x","volume":"10","author":"G Zhang","year":"2020","unstructured":"Zhang G, Ding C, Jiang X, Pan G, Wei X, Sun Y (2020) Chemical compositions and sources contribution of atmospheric particles at a typical steel industrial urban site. Scientific reports 10(1):7654. https:\/\/doi.org\/10.1038\/s41598-020-64519-x","journal-title":"Scientific reports"}],"container-title":["Journal of Comparative Physiology B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00360-026-01698-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00360-026-01698-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00360-026-01698-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:02:24Z","timestamp":1787680944000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00360-026-01698-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,27]]},"references-count":73,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["1698"],"URL":"https:\/\/doi.org\/10.1007\/s00360-026-01698-5","relation":{},"ISSN":["0174-1578","1432-136X"],"issn-type":[{"value":"0174-1578","type":"print"},{"value":"1432-136X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,27]]},"assertion":[{"value":"13 April 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}}]}}