{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T00:20:09Z","timestamp":1793665209609,"version":"4.5.1"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pediatr Surg Int"],"DOI":"10.1007\/s00383-025-06043-5","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T02:49:31Z","timestamp":1752806971000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Exosomes derived from colostrum and mature human breast milk protect against experimental necrotizing enterocolitis"],"prefix":"10.1007","volume":"41","author":[{"given":"Runnan","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mashriq","family":"Alganabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Yamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueni","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijing","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agostino","family":"Pierro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"issue":"3","key":"6043_CR1","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1056\/NEJMra1005408","volume":"364","author":"J Neu","year":"2011","unstructured":"Neu J, Walker WA (2011) Necrotizing enterocolitis. N Engl J Med 364(3):255\u2013264","journal-title":"N Engl J Med"},{"key":"6043_CR2","doi-asserted-by":"publisher","first-page":"F1000","DOI":"10.12688\/f1000research.6888.1","volume":"4","author":"A Zani","year":"2015","unstructured":"Zani A, Pierro A (2015) Necrotizing enterocolitis: controversies and challenges. F1000Res 4:F1000","journal-title":"F1000Res"},{"issue":"1","key":"6043_CR3","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1053\/j.semperi.2016.09.016","volume":"41","author":"D Maffei","year":"2017","unstructured":"Maffei D, Schanler RJ (2017) Human milk is the feeding strategy to prevent necrotizing enterocolitis! Semin Perinatol 41(1):36\u201340","journal-title":"Semin Perinatol"},{"issue":"18","key":"6043_CR4","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1001\/jama.2016.16144","volume":"316","author":"DL O'Connor","year":"2016","unstructured":"O\u2019Connor DL, Gibbins S, Kiss A, Bando N, Brennan-Donnan J, Ng E et al (2016) Effect of supplemental donor human milk compared with preterm formula on neurodevelopment of very low-birth-weight infants at 18 months: a randomized clinical trial. JAMA 316(18):1897\u20131905","journal-title":"JAMA"},{"key":"6043_CR5","doi-asserted-by":"publisher","first-page":"92","DOI":"10.3389\/fgene.2018.00092","volume":"9","author":"GC de la Torre","year":"2018","unstructured":"de la Torre GC, Goreham RV, Bech Serra JJ, Nann T, Kussmann M (2018) \u201cExosomics\u201d-a review of biophysics, biology and biochemistry of exosomes with a focus on human breast milk. Front Genet 9:92","journal-title":"Front Genet"},{"issue":"5","key":"6043_CR6","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1089\/bfm.2012.0067","volume":"7","author":"AI Eidelman","year":"2012","unstructured":"Eidelman AI (2012) Breastfeeding and the use of human milk: an analysis of the American academy of pediatrics 2012 breastfeeding policy statement. Breastfeed Med 7(5):323\u2013324","journal-title":"Breastfeed Med"},{"issue":"11","key":"6043_CR7","doi-asserted-by":"publisher","first-page":"166229","DOI":"10.1016\/j.bbadis.2021.166229","volume":"1867","author":"JCW de Jong","year":"2021","unstructured":"de Jong JCW, Ijssennagger N, van Mil SWC (2021) Breast milk nutrients driving intestinal epithelial layer maturation via wnt and notch signaling: Implications for necrotizing enterocolitis. Biochim Biophys Acta Mol Basis Dis 1867(11):166229","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"issue":"6478","key":"6043_CR8","doi-asserted-by":"publisher","first-page":"eaau6977","DOI":"10.1126\/science.aau6977","volume":"367","author":"R Kalluri","year":"2020","unstructured":"Kalluri R, LeBleu VS (2020) The biology, function, and biomedical applications of exosomes. Science 367(6478):eaau6977","journal-title":"Science"},{"issue":"10","key":"6043_CR9","doi-asserted-by":"publisher","first-page":"e2000845","DOI":"10.1002\/mnfr.202000845","volume":"65","author":"W Chen","year":"2021","unstructured":"Chen W, Chen X, Qian Y, Wang X, Zhou Y, Yan X et al (2021) Lipidomic profiling of human milk derived exosomes and their emerging roles in the prevention of necrotizing enterocolitis. Mol Nutr Food Res 65(10):e2000845","journal-title":"Mol Nutr Food Res"},{"issue":"5","key":"6043_CR10","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1016\/j.jpedsurg.2017.01.032","volume":"52","author":"A Hock","year":"2017","unstructured":"Hock A, Miyake H, Li B, Lee C, Ermini L, Koike Y et al (2017) Breast milk-derived exosomes promote intestinal epithelial cell growth. J Pediatr Surg 52(5):755\u2013759","journal-title":"J Pediatr Surg"},{"issue":"1","key":"6043_CR11","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.jpedsurg.2019.09.052","volume":"55","author":"C Pisano","year":"2020","unstructured":"Pisano C, Galley J, Elbahrawy M, Wang Y, Farrell A, Brigstock D et al (2020) Human breast milk-derived extracellular vesicles in the protection against experimental necrotizing enterocolitis. J Pediatr Surg 55(1):54\u201358","journal-title":"J Pediatr Surg"},{"issue":"12","key":"6043_CR12","doi-asserted-by":"publisher","first-page":"1363","DOI":"10.1007\/s00383-019-04562-6","volume":"35","author":"R Gao","year":"2019","unstructured":"Gao R, Zhang R, Qian T, Peng X, He W, Zheng S et al (2019) A comparison of exosomes derived from different periods breast milk on protecting against intestinal organoid injury. Pediatr Surg Int 35(12):1363\u20131368","journal-title":"Pediatr Surg Int"},{"issue":"1","key":"6043_CR13","doi-asserted-by":"publisher","first-page":"e0211431","DOI":"10.1371\/journal.pone.0211431","volume":"14","author":"B Li","year":"2019","unstructured":"Li B, Hock A, Wu RY, Minich A, Botts SR, Lee C et al (2019) Bovine milk-derived exosomes enhance goblet cell activity and prevent the development of experimental necrotizing enterocolitis. PLoS ONE 14(1):e0211431","journal-title":"PLoS ONE"},{"issue":"6","key":"6043_CR14","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1055\/s-2008-1038951","volume":"18","author":"A Zani","year":"2008","unstructured":"Zani A, Cordischi L, Cananzi M, De Coppi P, Smith VV, Eaton S et al (2008) Assessment of a neonatal rat model of necrotizing enterocolitis. Eur J Pediatr Surg 18(6):423\u2013426","journal-title":"Eur J Pediatr Surg"},{"issue":"1","key":"6043_CR15","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s00383-015-3813-x","volume":"32","author":"A Zani","year":"2016","unstructured":"Zani A, Zani-Ruttenstock E, Peyvandi F, Lee C, Li B, Pierro A (2016) A spectrum of intestinal injury models in neonatal mice. Pediatr Surg Int 32(1):65\u201370","journal-title":"Pediatr Surg Int"},{"issue":"10","key":"6043_CR16","doi-asserted-by":"publisher","first-page":"1933","DOI":"10.1016\/j.jpedsurg.2010.05.002","volume":"45","author":"A Radulescu","year":"2010","unstructured":"Radulescu A, Zhang H-Y, Yu X, Olson JK, Darbyshire AK, Chen Y et al (2010) Heparin-binding epidermal growth factor\u2013like growth factor overexpression in transgenic mice increases resistance to necrotizing enterocolitis. J Pediatr Surg 45(10):1933\u20131939","journal-title":"J Pediatr Surg"},{"issue":"10","key":"6043_CR17","doi-asserted-by":"publisher","first-page":"3769","DOI":"10.1039\/C7FO00539C","volume":"8","author":"KA Dingess","year":"2017","unstructured":"Dingess KA, de Waard M, Boeren S, Vervoort J, Lambers TT, van Goudoever JB et al (2017) Human milk peptides differentiate between the preterm and term infant and across varying lactational stages. Food Funct 8(10):3769\u20133782","journal-title":"Food Funct"},{"issue":"2","key":"6043_CR18","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/s00383-019-04599-7","volume":"36","author":"H Miyake","year":"2020","unstructured":"Miyake H, Lee C, Chusilp S, Bhalla M, Li B, Pitino M et al (2020) Human breast milk exosomes attenuate intestinal damage. Pediatr Surg Int 36(2):155\u2013163","journal-title":"Pediatr Surg Int"},{"key":"6043_CR19","doi-asserted-by":"publisher","first-page":"e1801247","DOI":"10.1002\/mnfr.201801247","volume":"63","author":"X Wang","year":"2019","unstructured":"Wang X, Yan X, Zhang L, Cai J, Zhou Y, Liu H et al (2019) Identification and peptidomic profiling of exosomes in preterm human milk: insights into necrotizing enterocolitis prevention. Mol Nutr Food Res 63:e1801247","journal-title":"Mol Nutr Food Res"},{"key":"6043_CR20","doi-asserted-by":"publisher","first-page":"658505","DOI":"10.3389\/fphar.2021.658505","volume":"12","author":"KK Ayyar","year":"2021","unstructured":"Ayyar KK, Moss AC (2021) Exosomes in Intestinal Inflammation. Front Pharmacol 12:658505","journal-title":"Front Pharmacol"},{"issue":"1","key":"6043_CR21","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.pcl.2012.10.002","volume":"60","author":"O Ballard","year":"2013","unstructured":"Ballard O, Morrow AL (2013) Human milk composition: nutrients and bioactive factors. Pediatr Clin North Am 60(1):49\u201374","journal-title":"Pediatr Clin North Am"},{"key":"6043_CR22","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700082","author":"Y Liao","year":"2017","unstructured":"Liao Y, Du X, Li J, L\u00f6nnerdal B (2017) Human milk exosomes and their microRNAs survive digestion in vitro and are taken up by human intestinal cells. Mol Nutr Food Res. https:\/\/doi.org\/10.1002\/mnfr.201700082","journal-title":"Mol Nutr Food Res"},{"issue":"34","key":"6043_CR23","doi-asserted-by":"publisher","first-page":"9477","DOI":"10.1021\/acs.jafc.9b02925","volume":"67","author":"MY Xie","year":"2019","unstructured":"Xie MY, Hou LJ, Sun JJ, Zeng B, Xi QY, Luo JY et al (2019) Porcine milk exosome MiRNAs attenuate LPS-induced apoptosis through inhibiting TLR4\/NF-\u03baB and p53 pathways in intestinal epithelial cells. J Agric Food Chem 67(34):9477\u20139491","journal-title":"J Agric Food Chem"},{"key":"6043_CR24","doi-asserted-by":"publisher","first-page":"113898","DOI":"10.1016\/j.bcp.2020.113898","volume":"175","author":"MY Xie","year":"2020","unstructured":"Xie MY, Chen T, Xi QY, Hou LJ, Luo JY, Zeng B et al (2020) Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage. Biochem Pharmacol 175:113898","journal-title":"Biochem Pharmacol"},{"issue":"1\u20132","key":"6043_CR25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1439-0396.2005.00614.x","volume":"90","author":"JW Blum","year":"2006","unstructured":"Blum JW (2006) Nutritional physiology of neonatal calves. J Anim Physiol Anim Nutr (Berl) 90(1\u20132):1\u201311","journal-title":"J Anim Physiol Anim Nutr (Berl)"},{"issue":"S1","key":"6043_CR26","doi-asserted-by":"publisher","first-page":"s133","DOI":"10.1017\/S1751731119003148","volume":"14","author":"HM Hammon","year":"2020","unstructured":"Hammon HM, Liermann W, Frieten D, Koch C (2020) Review: importance of colostrum supply and milk feeding intensity on gastrointestinal and systemic development in calves. Animal 14(S1):s133\u2013s143","journal-title":"Animal"},{"issue":"2","key":"6043_CR27","doi-asserted-by":"publisher","first-page":"312","DOI":"10.3390\/cells10020312","volume":"10","author":"A Venkatraman","year":"2021","unstructured":"Venkatraman A, Yu W, Nitkin C, Sampath V (2021) Intestinal stem cell development in the neonatal gut: pathways regulating development and relevance to necrotizing enterocolitis. Cells 10(2):312","journal-title":"Cells"},{"issue":"7575","key":"6043_CR28","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1038\/nature15382","volume":"526","author":"A Gregorieff","year":"2015","unstructured":"Gregorieff A, Liu Y, Inanlou MR, Khomchuk Y, Wrana JL (2015) Yap-dependent reprogramming of Lgr5(+) stem cells drives intestinal regeneration and cancer. Nature 526(7575):715\u2013718","journal-title":"Nature"}],"container-title":["Pediatric Surgery International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00383-025-06043-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00383-025-06043-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00383-025-06043-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T09:25:20Z","timestamp":1763630720000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00383-025-06043-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,18]]},"references-count":28,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["6043"],"URL":"https:\/\/doi.org\/10.1007\/s00383-025-06043-5","relation":{},"ISSN":["1437-9813"],"issn-type":[{"value":"1437-9813","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,18]]},"assertion":[{"value":"4 May 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 July 2025","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"218"}}