{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:46:44Z","timestamp":1791564404950,"version":"4.3.4"},"reference-count":11,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"GOSH Charity and LifeArc Translational Research Accelerator"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pediatr Surg Int"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Oesophageal substitution following atresia repair, caustic damage or cancer of the oesophagus can be challenging. We and others are working on engineering oesophageal tissue using a combination of decellularised oesophagi and cell injection. So far this has been achieved using highly operator-dependent techniques. This study aimed to establish a reproducible method for cell delivery into scaffolds.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To improve consistency, a stereotaxic robotic platform was adapted to deliver a suspension of porcine gelatin and cells in a 1:1 ratio. The scaffold was mounted on a 3D-printed rod linked to a stepper motor, enabling automated 36\u00b0 rotation for circumferential coverage. Two circumferential rows, each rotated 36\u00b0, with 3\u2009\u2212\u20092 points at 3-mm intervals, ensured even seeding. Injection depth was calibrated to target the inner layer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Cells injected robotically remained viable, with no significant difference from manual injection. Post-injection analyses confirmed cell viability and distribution within the scaffold.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Automated robotic injection provides a reliable, reproducible alternative to manual methods, reducing operator bias.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00383-026-06309-6","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T09:38:54Z","timestamp":1771580334000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Oesophageal tissue engineering: optimisation of stereotactic robotic cell injection in decellularised oesophageal scaffolds"],"prefix":"10.1007","volume":"42","author":[{"given":"Koji","family":"Yamada","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Perea Paizal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Canovai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casper","family":"Orens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Marcoccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Durkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Caciolli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Ieiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Eaton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Mantero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"De Coppi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Pellegrini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"issue":"6","key":"6309_CR1","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1136\/adc.68.6.743","volume":"68","author":"A Depaepe","year":"1993","unstructured":"Depaepe A, Dolk H, Lechat MF (1993) The epidemiology of tracheo-oesophageal fistula and oesophageal Atresia in Europe. 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