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Early diagnosis is essential for timely intervention and improved outcomes. This study evaluated accessible biomarkers for the diagnosis of BA, including serum gamma-glutamyl transferase (GGT) and ultrasonographic (US) parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective study included infants with suspected BA treated between 2014 and 2024, at a tertiary pediatric center in Brazil. Serum GGT levels and US parameters were analyzed and the diagnosis was confirmed by surgical findings. Diagnostic accuracy was assessed using a ROC curve and logistic regression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Sixty-seven infants were included, of whom 45 (67.2%) had confirmed BA. Median serum GGT levels were higher in BA cases (607 U\/L) than in non-BA cases (243 U\/L). ROC analysis identified a cutoff value of 311 U\/L, with a sensitivity of 82.2% and specificity of 68.2%. US parameters were present in all BA cases, with 100% sensitivity and 86% specificity (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Combining GGT with abnormal US findings increased specificity to 95.5%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Ultrasonographic parameters alone, as well as their combination with elevated serum GGT levels, demonstrated high diagnostic value for identification of biliary atresia in infants with neonatal cholestasis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00383-026-06484-6","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T09:12:01Z","timestamp":1781773921000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ultrasonographic and serum biomarkers for diagnosis of biliary atresia"],"prefix":"10.1007","volume":"42","author":[{"given":"Betina Carla","family":"Bertrand Sim\u00f5es","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivonete","family":"Siviero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Renata Rezende","family":"Penna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elazir Barbosa Mota","family":"Di Puglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra Val\u00e9ria Coelho","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Eduardo Ferreira","family":"Manso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"6484_CR1","doi-asserted-by":"publisher","first-page":"6601","DOI":"10.3390\/jcm11216601","volume":"11","author":"M Davenport","year":"2022","unstructured":"Davenport M, Madadi-Sanjani O, Chardot C et al (2022) Surgical and Medical Aspects of the Initial Treatment of Biliary Atresia: Position Paper. 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