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Choline serves in parenchyma formation by membrane phosphatidylcholine (PC), plasma transport of poly-unsaturated fatty acids (PUFA) via PC, and methylation processes via betaine. PUFA-PC concentrations are high in brain, liver and lung, and deficiency may result in developmental disorders. We compared different deuterated (D9-) choline components for kinetics of D9-choline, D9-betaine and D9-PC.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Prospective study (1\/2021\u201312\/2021) in 32 enterally fed preterm infants (28 0\/7\u201332 0\/7\u00a0weeks gestation). Patients were randomized to receive enterally a single dose of 2.7\u00a0mg\/kg D9-choline-equivalent as D9-choline chloride, D9-phosphoryl-choline, D9-glycerophosphorylcholine (D9-GPC) or D9-1-palmitoyl-2-oleoyl-PC(D9-POPC), followed by blood sampling at 1\u2009+\u200924\u00a0h or 12\u2009+\u200960\u00a0h after administration. Plasma concentrations were analyzed by tandem mass spectrometry. Results are expressed as median (25th\/75th percentile).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>At 1\u00a0h, plasma D9-choline was 1.8 (0.9\/2.2) \u00b5mol\/L, 1.3 (0.9\/1.5) \u00b5mol\/L and 1.2 (0.7\/1.4) \u00b5mol\/L for D9-choline chloride, D9-GPC and D9-phosphoryl-choline, respectively. D9-POPC did not result in plasma D9-choline. Plasma D9-betaine was maximal at 12\u00a0h, with lowest concentrations after D9-POPC. Maximum plasma D9-PC values at 12\u00a0h were the highest after D9-POPC (14.4 (9.1\/18.9) \u00b5mol\/L), compared to the other components (D9-choline chloride: 8.1 [5.6\/9.9] \u00b5mol\/L; D9-GPC: 8.4 (6.2\/10.3) \u00b5mol\/L; D9-phosphoryl-choline: 9.8 (8.6\/14.5) \u00b5mol\/L). Predominance of D9-PC comprising linoleic, rather than oleic acid, indicated fatty-acyl remodeling of administered D9-POPC prior to systemic delivery.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>D9-Choline chloride, D9-GPC and D9-phosphoryl-choline equally increased plasma D9-choline and D9-betaine. D9-POPC shifted metabolism from D9-betaine to D9-PC. Combined supplementation of GPC and (PO) PC may be best suited to optimize choline supply in preterm infants. Due to fatty acid remodeling of (PO) PC during its assimilation, PUFA co-supplementation with (PO) PC may increase PUFA-delivery to critical organs. This study was registered (22.01.2020) at the Deutsches Register Klinischer Studien (DRKS) (German Register for Clinical Studies), DRKS00020502.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Study registration<\/jats:title>\n                <jats:p>This study was registered at the Deutsches Register Klinischer Studien (DRKS) (German Register for Clinical Studies), DRKS00020502.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00394-022-03059-8","type":"journal-article","created":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T03:03:12Z","timestamp":1670036592000},"page":"1195-1205","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Choline supplementation for preterm infants: metabolism of four Deuterium-labeled choline compounds"],"prefix":"10.1007","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2839-3585","authenticated-orcid":false,"given":"Katrin A.","family":"B\u00f6ckmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Bernhard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Minarski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Shunova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Wiechers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian F.","family":"Poets","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Axel R.","family":"Franz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,12,3]]},"reference":[{"issue":"3","key":"3059_CR1","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1007\/s00394-018-1834-7","volume":"58","author":"W Bernhard","year":"2019","unstructured":"Bernhard W, Poets CF, Franz AR (2019) Choline and choline-related nutrients in regular and preterm infant growth. 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