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This study investigates how fiber molecular structure affects resistance to digestion using in vitro models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>High-, medium-, and low-molecular-weight (Mw) corn arabinoxylans, resistant maltodextrin, and inulin were characterized by carbohydrate composition following acid hydrolysis and analyzed for sugar release during simulated digestion using the standardized InfoGest protocol and rat small intestinal extract (RSIE).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>High-Mw corn arabinoxylan (208.8\u00a0kDa) remained largely stable, with minor, non-significant increases in glucose and galactose. Medium- (25.5\u00a0kDa) and low-Mw (2.5\u00a0kDa) arabinoxylans showed partial hydrolysis, with increases in trisaccharides and maltose and decreases in glucose and arabinose. Resistant maltodextrin (1.9\u00a0kDa) displayed significant increases in glucose, trisaccharides, and maltose. Inulin (0.75\u00a0kDa) remained mostly intact, with only partial decreases in fructose and sucrose. Sugar release during 4\u00a0h of simulated digestion followed these trends: high-Mw arabinoxylan released 11.1\u00a0mg\/g, medium-Mw released 5.20\u00a0mg\/g\/h, low-Mw released 9.84\u00a0mg\/g, resistant maltodextrin released 6.83\u00a0mg\/g\/h, and inulin released 59.9\u00a0mg\/g.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings demonstrate that fiber structural variations critically influence the degree of hydrolysis during digestion and the resulting simple sugar release.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00394-025-03853-0","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T10:28:27Z","timestamp":1765016907000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Impact of fiber molecular structure on resistance to digestion using the infogest and rat small intestine extract protocols"],"prefix":"10.1007","volume":"65","author":[{"given":"Fatma","family":"Boukid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"M\u00e9ndez-Albi\u00f1ana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"S\u00e1nchez-Baca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar","family":"Villamiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,6]]},"reference":[{"issue":"8","key":"3853_CR1","doi-asserted-by":"publisher","first-page":"2389","DOI":"10.1007\/S00394-015-1045-4","volume":"55","author":"MS Abell\u00e1n Ruiz","year":"2015","unstructured":"Abell\u00e1n Ruiz MS, Espinosa B, Contreras Fern\u00e1ndez MD, Luque Rubia CJ, S\u00e1nchez Ayll\u00f3n AJ, Aldeguer F, Garc\u00eda M, Garc\u00eda Santamar\u00eda C, L\u00f3pez Rom\u00e1n FJ (2015) Digestion-resistant maltodextrin effects on colonic transit time and stool weight: a randomized controlled clinical study. 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