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Its validation against dietary intake and objective biomarkers is limited. This study aimed to validate the FCS using 24-hour recalls, erythrocyte fatty acids, a long-term biomarker of fatty acids intake, and MedDietScore as a healthiness index.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Apparently healthy subjects were recruited. Two 24\u00a0h-recalls and a food-frequency questionnaire were administered. Erythrocyte fatty acids were determined by gas chromatography. Energy adjusted Spearman correlations were performed. In addition, simple and age\/sex\/BMI adjusted linear regression models were applied with FCS as dependent and energy-adjusted nutrients or biomarkers as independent variables.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      One hundred and six subjects (48 men, 44\u2009\u00b1\u200913 years; 58 women, 44\u2009\u00b1\u200914 years) participated. The mean FCS of the study participants was 55.9\u2009\u00b1\u20099.5. In fully adjusted models the FCS was positively associated with % monounsaturated fatty acids (% of total energy intake) (B\u2009=\u20090.401, 95% CI:0.170\u20130.632,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001), % fat (% of total energy intake) (B\u2009=\u20090.230, 95% CI:0.036\u20130.425,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02), vitamin C (B\u2009=\u20090.022, 95% CI:0.005\u20130.038,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01), \u0251-tocopherol (B\u2009=\u20090.427, 95% CI:0.0070\u20130.785,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02), vitamin K (B\u2009=\u20095.225, 95% CI:1.670\u20138.840,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.005), potassium intakes (B\u2009=\u20090.002, 95% CI:0.0008\u20130.004,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.04), and the MedDietScore (B\u2009=\u20090.805, 95% CI:0.473\u20131.137,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). It was also negatively associated with % carbohydrate intake (% of total energy intake) (B = \u2212\u00a00.209, 95% CI: \u2212\u00a00.391 to \u2212\u00a00.027,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.025). In addition, the FCS was associated with unsaturated\/ SFA ratio, dietary fiber\/carbohydrate ratio, and potassium\/sodium ratio after adjustment for age, sex, BMI and energy intake. The FCS was positively associated with the % erythrocyte EPA (B = 13.814, 95% CI:5.632\u201321.996,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001), DHA (B = 1.769, 95% CI:0.332\u20133.205,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01), omega-3 fatty acids (B = 1.380, 95% CI:0.333\u20132.428,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01) and the omega-3 index (B = 1.581, 95% CI: 0.358\u20132.805,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The correlations of the FCS with the MedDietScore, nutrients and erythrocyte fatty acids investigated suggest that FCS is a valid index encouraging its use for clinical and consumer guidance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00394-026-03912-0","type":"journal-article","created":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T10:53:45Z","timestamp":1772016825000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Validation of the food compass score through 24\u00a0h recalls and measurement of erythrocyte fatty acids in a mediterranean population"],"prefix":"10.1007","volume":"65","author":[{"given":"P.","family":"Detopoulou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Yannakoulia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Fragopoulou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Kalogeropoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Nomikos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Antonopoulou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"3912_CR1","doi-asserted-by":"publisher","first-page":"771","DOI":"10.3390\/nu14040771","volume":"14","author":"L Muzzioli","year":"2022","unstructured":"Muzzioli L, Penzavecchia C, Donini LM, Pinto A (2022) Are front-of-pack labels health policy tool?. 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