{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T20:37:57Z","timestamp":1793738277687,"version":"4.6.1"},"reference-count":72,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["HT94252310109"],"award-info":[{"award-number":["HT94252310109"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Basic Res Cardiol"],"DOI":"10.1007\/s00395-025-01112-1","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T17:07:00Z","timestamp":1746119220000},"page":"559-578","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A new model of heart failure with preserved ejection fraction induced by metabolic syndrome in Ossabaw miniature swine"],"prefix":"10.1007","volume":"120","author":[{"given":"Xian-Liang","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mouhamad","family":"Alloosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghui","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devendra K.","family":"Agrawal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh K.","family":"Kalra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Sturek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9160-8614","authenticated-orcid":false,"given":"Roberto","family":"Bolli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,1]]},"reference":[{"key":"1112_CR1","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1161\/circulationaha.121.057641","volume":"145","author":"RRE Abouleisa","year":"2022","unstructured":"Abouleisa RRE, Salama ABM, Ou Q, Tang XL, Solanki M, Guo Y, Nong Y, McNally L, Lorkiewicz PK, Kassem KM, Ahern BM, Choudhary K, Thomas R, Huang Y, Juhardeen HR, Siddique A, Ifthikar Z, Hammad SK, Elbaz AS, Ivey KN, Conklin DJ, Satin J, Hill BG, Srivastava D, Bolli R, Mohamed TMA (2022) Transient cell cycle induction in cardiomyocytes to treat subacute ischemic heart failure. Circulation 145:1339\u20131355. https:\/\/doi.org\/10.1161\/circulationaha.121.057641","journal-title":"Circulation"},{"key":"1112_CR2","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/s12967-018-1431-9","volume":"16","author":"JK Badin","year":"2018","unstructured":"Badin JK, Kole A, Stivers B, Progar V, Pareddy A, Alloosh M, Sturek M (2018) Alloxan-induced diabetes exacerbates coronary atherosclerosis and calcification in Ossabaw miniature swine with metabolic syndrome. J Transl Med 16:58. https:\/\/doi.org\/10.1186\/s12967-018-1431-9","journal-title":"J Transl Med"},{"key":"1112_CR3","doi-asserted-by":"publisher","first-page":"G746","DOI":"10.1152\/ajpgi.00485.2009","volume":"298","author":"LN Bell","year":"2010","unstructured":"Bell LN, Lee L, Saxena R, Bemis KG, Wang M, Theodorakis JL, Vuppalanchi R, Alloosh M, Sturek M, Chalasani N (2010) Serum proteomic analysis of diet-induced steatohepatitis and metabolic syndrome in the Ossabaw miniature swine. Am J Physiol Gastrointest Liver Physiol 298:G746-754. https:\/\/doi.org\/10.1152\/ajpgi.00485.2009","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"1112_CR4","doi-asserted-by":"publisher","first-page":"912","DOI":"10.1016\/j.yjmcc.2011.07.004","volume":"52","author":"ZC Berwick","year":"2012","unstructured":"Berwick ZC, Dick GM, Moberly SP, Kohr MC, Sturek M, Tune JD (2012) Contribution of voltage-dependent K\u207a channels to metabolic control of coronary blood flow. J Mol Cell Cardiol 52:912\u2013919. https:\/\/doi.org\/10.1016\/j.yjmcc.2011.07.004","journal-title":"J Mol Cell Cardiol"},{"key":"1112_CR5","unstructured":"BGI-shenzhen (2022) Genome assembly ASM2471841v1. https:\/\/www.ncbi.nlm.nih.gov\/datasets\/genome\/GCA_024718415.1\/. Accessed 4\/20\/2025."},{"key":"1112_CR6","doi-asserted-by":"publisher","first-page":"H966","DOI":"10.1152\/ajpheart.00876.2009","volume":"298","author":"L Borbouse","year":"2010","unstructured":"Borbouse L, Dick GM, Payne GA, Payne BD, Svendsen MC, Neeb ZP, Alloosh M, Bratz IN, Sturek M, Tune JD (2010) Contribution of BK(Ca) channels to local metabolic coronary vasodilation: effects of metabolic syndrome. Am J Physiol Heart Circ Physiol 298:H966-973. https:\/\/doi.org\/10.1152\/ajpheart.00876.2009","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"1112_CR7","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1016\/s0140-6736(23)02756-3","volume":"403","author":"P Campbell","year":"2024","unstructured":"Campbell P, Rutten FH, Lee MM, Hawkins NM, Petrie MC (2024) Heart failure with preserved ejection fraction: everything the clinician needs to know. Lancet 403:1083\u20131092. https:\/\/doi.org\/10.1016\/s0140-6736(23)02756-3","journal-title":"Lancet"},{"key":"1112_CR8","doi-asserted-by":"publisher","first-page":"197","DOI":"10.7570\/jomes23052","volume":"32","author":"WK Chan","year":"2023","unstructured":"Chan WK, Chuah KH, Rajaram RB, Lim LL, Ratnasingam J, Vethakkan SR (2023) Metabolic dysfunction-associated steatotic liver disease (MASLD): a state-of-the-art review. J Obes Metab Syndr 32:197\u2013213. https:\/\/doi.org\/10.7570\/jomes23052","journal-title":"J Obes Metab Syndr"},{"key":"1112_CR9","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1002\/ehf2.12536","volume":"7","author":"CJ Charles","year":"2020","unstructured":"Charles CJ, Lee P, Li RR, Yeung T, Ibraham Mazlan SM, Tay ZW, Abdurrachim D, Teo XQ, Wang WH, de Kleijn DPV, Cozzone PJ, Lam CSP, Richards AM (2020) A porcine model of heart failure with preserved ejection fraction: magnetic resonance imaging and metabolic energetics. ESC Heart Fail 7:92\u2013102. https:\/\/doi.org\/10.1002\/ehf2.12536","journal-title":"ESC Heart Fail"},{"key":"1112_CR10","doi-asserted-by":"publisher","first-page":"E366","DOI":"10.1152\/ajpendo.00413.2021","volume":"322","author":"GL Cluzel","year":"2022","unstructured":"Cluzel GL, Ryan PM, Herisson FM, Caplice NM (2022) High-fidelity porcine models of metabolic syndrome: a contemporary synthesis. Am J Physiol Endocrinol Metab 322:E366-e381. https:\/\/doi.org\/10.1152\/ajpendo.00413.2021","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"1112_CR11","doi-asserted-by":"publisher","first-page":"H892","DOI":"10.1152\/ajpheart.00287.2022","volume":"323","author":"G de Couto","year":"2022","unstructured":"de Couto G, Mesquita T, Wu X, Rajewski A, Huang F, Akhmerov A, Na N, Wu D, Wang Y, Li L, Tran M, Kilfoil P, Cingolani E, Marb\u00e1n E (2022) Cell therapy attenuates endothelial dysfunction in hypertensive rats with heart failure and preserved ejection fraction. Am J Physiol Heart Circ Physiol 323:H892-h903. https:\/\/doi.org\/10.1152\/ajpheart.00287.2022","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"1112_CR12","first-page":"35","volume":"56","author":"MC Dyson","year":"2006","unstructured":"Dyson MC, Alloosh M, Vuchetich JP, Mokelke EA, Sturek M (2006) Components of metabolic syndrome and coronary artery disease in female Ossabaw swine fed excess atherogenic diet. Comp Med 56:35\u201345","journal-title":"Comp Med"},{"key":"1112_CR13","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1093\/cvr\/cvp308","volume":"85","author":"JM Edwards","year":"2010","unstructured":"Edwards JM, Neeb ZP, Alloosh MA, Long X, Bratz IN, Peller CR, Byrd JP, Kumar S, Obukhov AG, Sturek M (2010) Exercise training decreases store-operated Ca2+entry associated with metabolic syndrome and coronary atherosclerosis. Cardiovasc Res 85:631\u2013640. https:\/\/doi.org\/10.1093\/cvr\/cvp308","journal-title":"Cardiovasc Res"},{"key":"1112_CR14","doi-asserted-by":"publisher","first-page":"H408","DOI":"10.1152\/ajpheart.00719.2023","volume":"326","author":"C Eickelmann","year":"2024","unstructured":"Eickelmann C, Lieder HR, Sturek M, Heusch G, Kleinbongard P (2024) Differences in vasomotor function of mesenteric arteries between Ossabaw minipigs with predisposition to metabolic syndrome and G\u00f6ttingen minipigs. Am J Physiol Heart Circ Physiol 326:H408-h417. https:\/\/doi.org\/10.1152\/ajpheart.00719.2023","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"1112_CR15","doi-asserted-by":"publisher","first-page":"H1687","DOI":"10.1152\/ajpheart.00610.2011","volume":"301","author":"CA Emter","year":"2011","unstructured":"Emter CA, Tharp DL, Ivey JR, Ganjam VK, Bowles DK (2011) Low-intensity interval exercise training attenuates coronary vascular dysfunction and preserves Ca2\u207a-sensitive K\u207a current in miniature swine with LV hypertrophy. Am J Physiol Heart Circ Physiol 301:H1687-1694. https:\/\/doi.org\/10.1152\/ajpheart.00610.2011","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"1112_CR16","unstructured":"Ensembl (2024) Ossabaw miniature (Sus scrofa) genome assembly (ASM2471841v1). https:\/\/useast.ensembl.org\/Sus_scrofa_ossabaw_miniature\/Info\/Index. Accessed 4\/20\/2025"},{"key":"1112_CR17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2024.150525","volume":"737","author":"Y Feng","year":"2024","unstructured":"Feng Y, Xin Y, Tang W, Zhang P, Jiang Y, Li H, Gong Y, Chen F, Xu Z, Liu Z, Gao L (2024) Repeat administration of human umbilical cord mesenchymal stem cells improves left ventricular diastolic function in mice with heart failure with preserved ejection fraction. Biochem Biophys Res Commun 737:150525. https:\/\/doi.org\/10.1016\/j.bbrc.2024.150525","journal-title":"Biochem Biophys Res Commun"},{"key":"1112_CR18","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1097\/MPA.0b013e3182061583","volume":"40","author":"AM Fullenkamp","year":"2011","unstructured":"Fullenkamp AM, Bell LN, Robbins RD, Lee L, Saxena R, Alloosh M, Klaunig JE, Mirmira RG, Sturek M, Chalasani N (2011) Effect of different obesogenic diets on pancreatic histology in Ossabaw miniature swine. Pancreas 40:438\u2013443. https:\/\/doi.org\/10.1097\/MPA.0b013e3182061583","journal-title":"Pancreas"},{"key":"1112_CR19","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.jacbts.2016.01.003","volume":"1","author":"R Gallet","year":"2016","unstructured":"Gallet R, de Couto G, Simsolo E, Valle J, Sun B, Liu W, Tseliou E, Zile MR, Marb\u00e1n E (2016) Cardiosphere-derived cells reverse heart failure with preserved ejection fraction (HFpEF) in rats by decreasing fibrosis and inflammation. JACC Basic Transl Sci 1:14\u201328. https:\/\/doi.org\/10.1016\/j.jacbts.2016.01.003","journal-title":"JACC Basic Transl Sci"},{"key":"1112_CR20","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1007\/s00395-023-00995-2","volume":"118","author":"R Gallet","year":"2023","unstructured":"Gallet R, Su JB, Corboz D, Chiaroni PM, Biz\u00e9 A, Dai J, Panel M, Boucher M, Pallot G, Brehat J, Sambin L, Thery G, Mouri N, de Pommereau A, Denormandie P, Germain S, Lacampagne A, Teiger E, Marb\u00e1n E, Ghaleh B (2023) Three-vessel coronary infusion of cardiosphere-derived cells for the treatment of heart failure with preserved ejection fraction in a pre-clinical pig model. Basic Res Cardiol 118:26. https:\/\/doi.org\/10.1007\/s00395-023-00995-2","journal-title":"Basic Res Cardiol"},{"key":"1112_CR21","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1007\/s10741-022-10232-y","volume":"28","author":"L Grosman-Rimon","year":"2023","unstructured":"Grosman-Rimon L, Wright E, Sabovich S, Rimon J, Gleitman S, Sudarsky D, Lubovich A, Gabizon I, Lalonde SD, Tsuk S, McDonald MA, Rao V, Gutterman D, Jorde UP, Carasso S, Kachel E (2023) Relationships among norepinephrine levels, exercise capacity, and chronotropic responses in heart failure patients. Heart Fail Rev 28:35\u201345. https:\/\/doi.org\/10.1007\/s10741-022-10232-y","journal-title":"Heart Fail Rev"},{"key":"1112_CR22","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1038\/s41572-024-00540-y","volume":"10","author":"CE Hamo","year":"2024","unstructured":"Hamo CE, DeJong C, Hartshorne-Evans N, Lund LH, Shah SJ, Solomon S, Lam CSP (2024) Heart failure with preserved ejection fraction. Nat Rev Dis Primers 10:55. https:\/\/doi.org\/10.1038\/s41572-024-00540-y","journal-title":"Nat Rev Dis Primers"},{"key":"1112_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00395-022-00909-8","volume":"117","author":"G Heusch","year":"2022","unstructured":"Heusch G (2022) Coronary blood flow in heart failure: cause, consequence and bystander. Basic Res Cardiol 117:1. https:\/\/doi.org\/10.1007\/s00395-022-00909-8","journal-title":"Basic Res Cardiol"},{"key":"1112_CR24","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1002\/ejhf.270","volume":"17","author":"S Kadota","year":"2015","unstructured":"Kadota S, Carey J, Reinecke H, Leggett J, Teichman S, Laflamme MA, Murry CE, Regnier M, Mahairas GG (2015) Ribonucleotide reductase-mediated increase in dATP improves cardiac performance via myosin activation in a large animal model of heart failure. Eur J Heart Fail 17:772\u2013781. https:\/\/doi.org\/10.1002\/ejhf.270","journal-title":"Eur J Heart Fail"},{"key":"1112_CR25","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1007\/s00395-022-00965-0","volume":"117","author":"P Kleinbongard","year":"2022","unstructured":"Kleinbongard P, Lieder HR, Skyschally A, Alloosh M, G\u00f6decke A, Rahmann S, Sturek M, Heusch G (2022) Non-responsiveness to cardioprotection by ischaemic preconditioning in Ossabaw minipigs with genetic predisposition to, but without the phenotype of the metabolic syndrome. Basic Res Cardiol 117:58. https:\/\/doi.org\/10.1007\/s00395-022-00965-0","journal-title":"Basic Res Cardiol"},{"key":"1112_CR26","doi-asserted-by":"publisher","DOI":"10.3791\/51269","author":"S Koudstaal","year":"2014","unstructured":"Koudstaal S, Jansen-Lorkeers S, Gho JM, van Hout GP, Jansen MS, Gr\u00fcndeman PF, Pasterkamp G, Doevendans PA, Hoefer IE, Chamuleau SA (2014) Myocardial infarction and functional outcome assessment in pigs. J Vis Exp. https:\/\/doi.org\/10.3791\/51269","journal-title":"J Vis Exp"},{"key":"1112_CR27","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1002\/hep.22904","volume":"50","author":"L Lee","year":"2009","unstructured":"Lee L, Alloosh M, Saxena R, Van Alstine W, Watkins BA, Klaunig JE, Sturek M, Chalasani N (2009) Nutritional model of steatohepatitis and metabolic syndrome in the Ossabaw miniature swine. Hepatology 50:56\u201367. https:\/\/doi.org\/10.1002\/hep.22904","journal-title":"Hepatology"},{"key":"1112_CR28","doi-asserted-by":"publisher","first-page":"1099453","DOI":"10.3389\/fcvm.2023.1099453","volume":"10","author":"K Li","year":"2023","unstructured":"Li K, Cardoso C, Moctezuma-Ramirez A, Elgalad A, Perin E (2023) Evaluation of large animal models for preclinical studies of heart failure with preserved ejection fraction using clinical score systems. Front Cardiovasc Med 10:1099453. https:\/\/doi.org\/10.3389\/fcvm.2023.1099453","journal-title":"Front Cardiovasc Med"},{"key":"1112_CR29","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1007\/s00395-017-0643-0","volume":"112","author":"W Li","year":"2017","unstructured":"Li W, Chen X, Riley AM, Hiett SC, Temm CJ, Beli E, Long X, Chakraborty S, Alloosh M, White FA, Grant MB, Sturek M, Obukhov AG (2017) Long-term spironolactone treatment reduces coronary TRPC expression, vasoconstriction, and atherosclerosis in metabolic syndrome pigs. Basic Res Cardiol 112:54. https:\/\/doi.org\/10.1007\/s00395-017-0643-0","journal-title":"Basic Res Cardiol"},{"key":"1112_CR30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124173","volume":"10","author":"T Liang","year":"2015","unstructured":"Liang T, Alloosh M, Bell LN, Fullenkamp A, Saxena R, Van Alstine W, Bybee P, Werling K, Sturek M, Chalasani N, Masuoka HC (2015) Liver injury and fibrosis induced by dietary challenge in the Ossabaw miniature Swine. PLoS ONE 10:e0124173. https:\/\/doi.org\/10.1371\/journal.pone.0124173","journal-title":"PLoS ONE"},{"key":"1112_CR31","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.ijcard.2023.05.026","volume":"386","author":"HR Lieder","year":"2023","unstructured":"Lieder HR, Adam V, Skyschally A, Sturek M, Kleinbongard P, Heusch G (2023) Attenuation of ST-segment elevation by ischemic preconditioning: Reflection of cardioprotection in G\u00f6ttingen but not in Ossabaw minipigs. Int J Cardiol 386:109\u2013117. https:\/\/doi.org\/10.1016\/j.ijcard.2023.05.026","journal-title":"Int J Cardiol"},{"key":"1112_CR32","doi-asserted-by":"publisher","first-page":"H1365","DOI":"10.1152\/ajpheart.00580.2022","volume":"323","author":"HR Lieder","year":"2022","unstructured":"Lieder HR, Skyschally A, Sturek M, Heusch G, Kleinbongard P (2022) Remote ischemic conditioning in Ossabaw minipigs induces the release of humoral cardioprotective triggers, but the myocardium does not respond with reduced infarct size. Am J Physiol Heart Circ Physiol 323:H1365-h1375. https:\/\/doi.org\/10.1152\/ajpheart.00580.2022","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"1112_CR33","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1124\/jpet.110.170803","volume":"335","author":"X Long","year":"2010","unstructured":"Long X, Mokelke EA, Neeb ZP, Alloosh M, Edwards JM, Sturek M (2010) Adenosine receptor regulation of coronary blood flow in Ossabaw miniature swine. J Pharmacol Exp Ther 335:781\u2013787. https:\/\/doi.org\/10.1124\/jpet.110.170803","journal-title":"J Pharmacol Exp Ther"},{"key":"1112_CR34","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1152\/japplphysiol.01059.2012","volume":"114","author":"KD Marshall","year":"1985","unstructured":"Marshall KD, Muller BN, Krenz M, Hanft LM, McDonald KS, Dellsperger KC, Emter CA (1985) Emter CA (2013) Heart failure with preserved ejection fraction: chronic low-intensity interval exercise training preserves myocardial O2 balance and diastolic function. J Appl Physiol 114:131\u2013147. https:\/\/doi.org\/10.1152\/japplphysiol.01059.2012","journal-title":"J Appl Physiol"},{"key":"1112_CR35","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1016\/j.soard.2022.10.023","volume":"19","author":"R Mazor","year":"2023","unstructured":"Mazor R, Babkin A, Littrup PJ, Alloush M, Sturek M, Byrd JP, Hernandez E, Bays H, Grunvald E, Mattar SG (2023) Mesenteric fat cryolipolysis attenuates insulin resistance in the Ossabaw swine model of the metabolic syndrome. Surg Obes Relat Dis 19:374\u2013383. https:\/\/doi.org\/10.1016\/j.soard.2022.10.023","journal-title":"Surg Obes Relat Dis"},{"key":"1112_CR36","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1111\/joa.13117","volume":"236","author":"C M\u00fchlfeld","year":"2020","unstructured":"M\u00fchlfeld C, Rajces A, Manninger M, Alogna A, Wierich MC, Scherr D, Post H, Schipke J (2020) A transmural gradient of myocardial remodeling in early-stage heart failure with preserved ejection fraction in the pig. J Anat 236:531\u2013539. https:\/\/doi.org\/10.1111\/joa.13117","journal-title":"J Anat"},{"key":"1112_CR37","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.echo.2016.01.011","volume":"29","author":"SF Nagueh","year":"2016","unstructured":"Nagueh SF, Smiseth OA, Appleton CP, Byrd BF 3rd, Dokainish H, Edvardsen T, Flachskampf FA, Gillebert TC, Klein AL, Lancellotti P, Marino P, Oh JK, Popescu BA, Waggoner AD (2016) Recommendations for the evaluation of left ventricular diastolic function by echocardiography: an update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging. J Am Soc Echocardiogr 29:277\u2013314. https:\/\/doi.org\/10.1016\/j.echo.2016.01.011","journal-title":"J Am Soc Echocardiogr"},{"key":"1112_CR38","doi-asserted-by":"publisher","first-page":"112","DOI":"10.4239\/wjd.v7.i6.112","volume":"7","author":"K Nakajima","year":"2016","unstructured":"Nakajima K (2016) Low serum amylase and obesity, diabetes and metabolic syndrome: a novel interpretation. World J Diabetes 7:112\u2013121. https:\/\/doi.org\/10.4239\/wjd.v7.i6.112","journal-title":"World J Diabetes"},{"key":"1112_CR39","first-page":"300","volume":"60","author":"ZP Neeb","year":"2010","unstructured":"Neeb ZP, Edwards JM, Alloosh M, Long X, Mokelke EA, Sturek M (2010) Metabolic syndrome and coronary artery disease in Ossabaw compared with Yucatan swine. Comp Med 60:300\u2013315","journal-title":"Comp Med"},{"key":"1112_CR40","volume-title":"Applied linear statistical models","year":"1990","unstructured":"Neter J, Wasserman W, Kutner H (eds) (1990) Applied linear statistical models. Irwin, Boston"},{"key":"1112_CR41","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1016\/j.jacbts.2019.02.004","volume":"4","author":"TD Olver","year":"2019","unstructured":"Olver TD, Edwards JC, Jurrissen TJ, Veteto AB, Jones JL, Gao C, Rau C, Warren CM, Klutho PJ, Alex L, Ferreira-Nichols SC, Ivey JR, Thorne PK, McDonald KS, Krenz M, Baines CP, Solaro RJ, Wang Y, Ford DA, Domeier TL, Padilla J, Rector RS, Emter CA (2019) Western diet-fed, aortic-banded Ossabaw Swine: A PRECLINICAL MODEL OF CARDIO-METABOLIC HEART FAilure. JACC Basic Transl Sci 4:404\u2013421. https:\/\/doi.org\/10.1016\/j.jacbts.2019.02.004","journal-title":"JACC Basic Transl Sci"},{"key":"1112_CR42","first-page":"53","volume":"53","author":"CR Otis","year":"2003","unstructured":"Otis CR, Wamhoff BR, Sturek M (2003) Hyperglycemia-induced insulin resistance in diabetic dyslipidemic Yucatan swine. Comp Med 53:53\u201364","journal-title":"Comp Med"},{"key":"1112_CR43","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1152\/japplphysiol.00146.2019","volume":"127","author":"A Ouyang","year":"1985","unstructured":"Ouyang A, Olver TD, Emter CA (1985) Fleenor BS (2019) Chronic exercise training prevents coronary artery stiffening in aortic-banded miniswine: role of perivascular adipose-derived advanced glycation end products. J Appl Physiol 127:816\u2013827. https:\/\/doi.org\/10.1152\/japplphysiol.00146.2019","journal-title":"J Appl Physiol"},{"key":"1112_CR44","doi-asserted-by":"publisher","first-page":"H1100","DOI":"10.1152\/ajpheart.00697.2019","volume":"318","author":"DC Poole","year":"2020","unstructured":"Poole DC, Copp SW, Colburn TD, Craig JC, Allen DL, Sturek M, O\u2019Leary DS, Zucker IH, Musch TI (2020) Guidelines for animal exercise and training protocols for cardiovascular studies. Am J Physiol Heart Circ Physiol 318:H1100-h1138. https:\/\/doi.org\/10.1152\/ajpheart.00697.2019","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"1112_CR45","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1001\/jama.2023.2020","volume":"329","author":"MM Redfield","year":"2023","unstructured":"Redfield MM, Borlaug BA (2023) Heart failure with preserved ejection fraction: a review. JAMA 329:827\u2013838. https:\/\/doi.org\/10.1001\/jama.2023.2020","journal-title":"JAMA"},{"key":"1112_CR46","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1186\/s12968-016-0283-9","volume":"18","author":"U Reiter","year":"2016","unstructured":"Reiter U, Reiter G, Manninger M, Adelsmayr G, Schipke J, Alogna A, Rajces A, Stalder AF, Greiser A, M\u00fchlfeld C, Scherr D, Post H, Pieske B, Fuchsj\u00e4ger M (2016) Early-stage heart failure with preserved ejection fraction in the pig: a cardiovascular magnetic resonance study. J Cardiovasc Magn Reson 18:63. https:\/\/doi.org\/10.1186\/s12968-016-0283-9","journal-title":"J Cardiovasc Magn Reson"},{"key":"1112_CR47","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1093\/stcltm\/szab004","volume":"11","author":"AC Rieger","year":"2022","unstructured":"Rieger AC, Tompkins BA, Natsumeda M, Florea V, Banerjee MN, Rodriguez J, Rosado M, Porras V, Valasaki K, Takeuchi LM, Collon K, Desai S, Bellio MA, Khan A, Kashikar ND, Landin AM, Hardin DV, Rodriguez DA, Balkan W, Hare JM, Schulman IH (2022) Allogeneic cell combination therapy ameliorates chronic kidney disease-induced heart failure with preserved ejection fraction. Stem Cells Transl Med 11:59\u201372. https:\/\/doi.org\/10.1093\/stcltm\/szab004","journal-title":"Stem Cells Transl Med"},{"key":"1112_CR48","unstructured":"SAS Institute I (ed) (1988) SAS.STAT user's guide. SAS Institute, Cary, NC"},{"key":"1112_CR49","doi-asserted-by":"publisher","first-page":"H1407","DOI":"10.1152\/ajpheart.00542.2015","volume":"309","author":"M Schwarzl","year":"2015","unstructured":"Schwarzl M, Hamdani N, Seiler S, Alogna A, Manninger M, Reilly S, Zirngast B, Kirsch A, Steendijk P, Verderber J, Zweiker D, Eller P, H\u00f6fler G, Schauer S, Eller K, Maechler H, Pieske BM, Linke WA, Casadei B, Post H (2015) A porcine model of hypertensive cardiomyopathy: implications for heart failure with preserved ejection fraction. Am J Physiol Heart Circ Physiol 309:H1407-1418. https:\/\/doi.org\/10.1152\/ajpheart.00542.2015","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"1112_CR50","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1161\/circulationaha.119.041886","volume":"141","author":"SJ Shah","year":"2020","unstructured":"Shah SJ, Borlaug BA, Kitzman DW, McCulloch AD, Blaxall BC, Agarwal R, Chirinos JA, Collins S, Deo RC, Gladwin MT, Granzier H, Hummel SL, Kass DA, Redfield MM, Sam F, Wang TJ, Desvigne-Nickens P, Adhikari BB (2020) Research priorities for heart failure with preserved ejection fraction: national heart, lung, and blood institute working group summary. Circulation 141:1001\u20131026. https:\/\/doi.org\/10.1161\/circulationaha.119.041886","journal-title":"Circulation"},{"key":"1112_CR51","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/j.hfc.2014.04.008","volume":"10","author":"SJ Shah","year":"2014","unstructured":"Shah SJ, Katz DH, Deo RC (2014) Phenotypic spectrum of heart failure with preserved ejection fraction. Heart Fail Clin 10:407\u2013418. https:\/\/doi.org\/10.1016\/j.hfc.2014.04.008","journal-title":"Heart Fail Clin"},{"key":"1112_CR52","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.jacbts.2020.11.012","volume":"6","author":"TE Sharp 3rd","year":"2021","unstructured":"Sharp TE 3rd, Scarborough AL, Li Z, Polhemus DJ, Hidalgo HA, Schumacher JD, Matsuura TR, Jenkins JS, Kelly DP, Goodchild TT, Lefer DJ (2021) Novel G\u00f6ttingen Miniswine model of heart failure with preserved ejection fraction integrating multiple comorbidities. JACC Basic Transl Sci 6:154\u2013170. https:\/\/doi.org\/10.1016\/j.jacbts.2020.11.012","journal-title":"JACC Basic Transl Sci"},{"key":"1112_CR53","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1093\/cvr\/cvy038","volume":"114","author":"O Sorop","year":"2018","unstructured":"Sorop O, Heinonen I, van Kranenburg M, van de Wouw J, de Beer VJ, Nguyen ITN, Octavia Y, van Duin RWB, Stam K, van Geuns RJ, Wielopolski PA, Krestin GP, van den Meiracker AH, Verjans R, van Bilsen M, Danser AHJ, Paulus WJ, Cheng C, Linke WA, Joles JA, Verhaar MC, van der Velden J, Merkus D, Duncker DJ (2018) Multiple common comorbidities produce left ventricular diastolic dysfunction associated with coronary microvascular dysfunction, oxidative stress, and myocardial stiffening. Cardiovasc Res 114:954\u2013964. https:\/\/doi.org\/10.1093\/cvr\/cvy038","journal-title":"Cardiovasc Res"},{"key":"1112_CR54","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1152\/japplphysiol.00373.2011","volume":"111","author":"M Sturek","year":"2011","unstructured":"Sturek M (2011) Ca2+ regulatory mechanisms of exercise protection against coronary artery disease in metabolic syndrome and diabetes. J Appl Physiol 111:573\u2013586. https:\/\/doi.org\/10.1152\/japplphysiol.00373.2011","journal-title":"J Appl Physiol"},{"key":"1112_CR55","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1146\/annurev-bioeng-082919-053009","volume":"22","author":"M Sturek","year":"2020","unstructured":"Sturek M, Alloosh M, Sellke FW (2020) Swine disease models for optimal vascular engineering. Annu Rev Biomed Eng 22:25\u201349. https:\/\/doi.org\/10.1146\/annurev-bioeng-082919-053009","journal-title":"Annu Rev Biomed Eng"},{"key":"1112_CR56","unstructured":"Sturek M, Tune J, Alloosh M (2015) Ossabaw Island miniature swine: metabolic syndrome and cardiovascular assessment. In: Swine in the laboratory: surgery, anesthesia, imaging, and experimental techniques, pp 451\u2013465"},{"key":"1112_CR57","doi-asserted-by":"publisher","first-page":"1091","DOI":"10.1161\/CIRCRESAHA.115.307647","volume":"118","author":"XL Tang","year":"2016","unstructured":"Tang XL, Li Q, Rokosh G, Sanganalmath SK, Chen N, Ou Q, Stowers H, Hunt G, Bolli R (2016) Long-Term Outcome of Administration of c-kit(POS) cardiac progenitor cells after acute myocardial infarction: transplanted cells do not become cardiomyocytes, but structural and functional improvement and proliferation of endogenous cells persist for at least one year. Circ Res 118:1091\u20131105. https:\/\/doi.org\/10.1161\/CIRCRESAHA.115.307647","journal-title":"Circ Res"},{"key":"1112_CR58","doi-asserted-by":"publisher","DOI":"10.1161\/jaha.117.007400","author":"XL Tang","year":"2018","unstructured":"Tang XL, Nakamura S, Li Q, Wysoczynski M, Gumpert AM, Wu WJ, Hunt G, Stowers H, Ou Q, Bolli R (2018) Repeated Administrations of Cardiac Progenitor cells are superior to a single administration of an equivalent cumulative dose. J Am Heart Assoc. https:\/\/doi.org\/10.1161\/jaha.117.007400","journal-title":"J Am Heart Assoc"},{"key":"1112_CR59","doi-asserted-by":"publisher","first-page":"2429","DOI":"10.1007\/s12015-023-10590-6","volume":"19","author":"XL Tang","year":"2023","unstructured":"Tang XL, Nasr M, Zheng S, Zoubul T, Stephan JK, Uchida S, Singhal R, Khan A, Gumpert A, Bolli R, Wysoczynski M (2023) Bone Marrow and Wharton\u2019s Jelly Mesenchymal stromal cells are ineffective for myocardial repair in an immunodeficient rat model of chronic ischemic cardiomyopathy. Stem Cell Rev Rep 19:2429\u20132446. https:\/\/doi.org\/10.1007\/s12015-023-10590-6","journal-title":"Stem Cell Rev Rep"},{"key":"1112_CR60","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1161\/CIRCULATIONAHA.109.871905","volume":"121","author":"XL Tang","year":"2010","unstructured":"Tang XL, Rokosh G, Sanganalmath SK, Yuan F, Sato H, Mu J, Dai S, Li C, Chen N, Peng Y, Dawn B, Hunt G, Leri A, Kajstura J, Tiwari S, Shirk G, Anversa P, Bolli R (2010) Intracoronary administration of cardiac progenitor cells alleviates left ventricular dysfunction in rats with a 30-day-old infarction. Circulation 121:293\u2013305. https:\/\/doi.org\/10.1161\/CIRCULATIONAHA.109.871905","journal-title":"Circulation"},{"key":"1112_CR61","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1007\/s11010-021-04283-2","volume":"477","author":"XL Tang","year":"2022","unstructured":"Tang XL, Wysoczynski M, Gumpert AM, Li Y, Wu WJ, Li H, Stowers H, Bolli R (2022) Effect of intravenous cell therapy in rats with old myocardial infarction. Mol Cell Biochem 477:431\u2013444. https:\/\/doi.org\/10.1007\/s11010-021-04283-2","journal-title":"Mol Cell Biochem"},{"key":"1112_CR62","doi-asserted-by":"publisher","first-page":"1939","DOI":"10.1093\/cvr\/cvae173","volume":"120","author":"XL Tang","year":"2024","unstructured":"Tang XL, Wysoczynski M, Gumpert AM, Solanki M, Li Y, Wu WJ, Zheng S, Ruble H, Li H, Stowers H, Zheng S, Ou Q, Tanveer N, Slezak J, Kalra DK, Bolli R (2024) Intravenous infusions of mesenchymal stromal cells have cumulative beneficial effects in a porcine model of chronic ischaemic cardiomyopathy. Cardiovasc Res 120:1939\u20131952. https:\/\/doi.org\/10.1093\/cvr\/cvae173","journal-title":"Cardiovasc Res"},{"key":"1112_CR63","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.1939-1676.1993.tb01015.x","volume":"7","author":"WP Thomas","year":"1993","unstructured":"Thomas WP, Gaber CE, Jacobs GJ, Kaplan PM, Lombard CW, Moise NS, Moses BL (1993) Recommendations for standards in transthoracic two-dimensional echocardiography in the dog and cat. Echocardiography Committee of the Specialty of Cardiology, American College of Veterinary Internal Medicine. J Vet Intern Med 7:247\u2013252. https:\/\/doi.org\/10.1111\/j.1939-1676.1993.tb01015.x","journal-title":"J Vet Intern Med"},{"key":"1112_CR64","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.116.308937","author":"Y Tokita","year":"2016","unstructured":"Tokita Y, Tang XL, Li Q, Wysoczynski M, Hong KU, Nakamura S, Wu W, Xie W, Li D, Hunt G, Ou Q, Stowers H, Bolli R (2016) Repeated administrations of cardiac progenitor cells are markedly more effective than a single administration: a new paradigm in cell therapy. Circ Res. https:\/\/doi.org\/10.1161\/circresaha.116.308937","journal-title":"Circ Res"},{"key":"1112_CR65","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1152\/japplphysiol.00604.2012","volume":"113","author":"AJ Trask","year":"2012","unstructured":"Trask AJ, Katz PS, Kelly AP, Galantowicz ML, Cismowski MJ, West TA, Neeb ZP, Berwick ZC, Goodwill AG, Alloosh M, Tune JD, Sturek M, Lucchesi PA (2012) Dynamic micro- and macrovascular remodeling in coronary circulation of obese Ossabaw pigs with metabolic syndrome. J Appl Physiol 113:1128\u20131140. https:\/\/doi.org\/10.1152\/japplphysiol.00604.2012","journal-title":"J Appl Physiol"},{"key":"1112_CR66","doi-asserted-by":"publisher","first-page":"e93","DOI":"10.1161\/cir.0000000000001123","volume":"147","author":"CW Tsao","year":"2023","unstructured":"Tsao CW, Aday AW, Almarzooq ZI, Anderson CAM, Arora P, Avery CL, Baker-Smith CM, Beaton AZ, Boehme AK, Buxton AE, Commodore-Mensah Y, Elkind MSV, Evenson KR, Eze-Nliam C, Fugar S, Generoso G, Heard DG, Hiremath S, Ho JE, Kalani R, Kazi DS, Ko D, Levine DA, Liu J, Ma J, Magnani JW, Michos ED, Mussolino ME, Navaneethan SD, Parikh NI, Poudel R, Rezk-Hanna M, Roth GA, Shah NS, St-Onge MP, Thacker EL, Virani SS, Voeks JH, Wang NY, Wong ND, Wong SS, Yaffe K, Martin SS (2023) Heart disease and stroke statistics-2023 update: a report from the American Heart Association. Circulation 147:e93\u2013e621. https:\/\/doi.org\/10.1161\/cir.0000000000001123","journal-title":"Circulation"},{"key":"1112_CR67","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1007\/s00395-022-00958-z","volume":"117","author":"JD Tune","year":"2022","unstructured":"Tune JD, Goodwill AG, Baker HE, Dick GM, Warne CM, Tucker SM, Essajee SI, Bailey CA, Klasing JA, Russell JJ, McCallinhart PE, Trask AJ, Bender SB (2022) Chronic high-rate pacing induces heart failure with preserved ejection fraction-like phenotype in Ossabaw swine. Basic Res Cardiol 117:50. https:\/\/doi.org\/10.1007\/s00395-022-00958-z","journal-title":"Basic Res Cardiol"},{"key":"1112_CR68","doi-asserted-by":"publisher","first-page":"844","DOI":"10.1016\/j.jacbts.2021.12.009","volume":"7","author":"WB van Ham","year":"2022","unstructured":"van Ham WB, Kessler EL, Oerlemans M, Handoko ML, Sluijter JPG, van Veen TAB, den Ruijter HM, de Jager SCA (2022) Clinical phenotypes of heart failure with preserved ejection fraction to select preclinical animal models. JACC Basic Transl Sci 7:844\u2013857. https:\/\/doi.org\/10.1016\/j.jacbts.2021.12.009","journal-title":"JACC Basic Transl Sci"},{"key":"1112_CR69","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1002\/hep.28431","volume":"64","author":"ZM Younossi","year":"2016","unstructured":"Younossi ZM, Koenig AB, Abdelatif D, Fazel Y, Henry L, Wymer M (2016) Global epidemiology of nonalcoholic fatty liver disease-meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology 64:73\u201384. https:\/\/doi.org\/10.1002\/hep.28431","journal-title":"Hepatology"},{"key":"1112_CR70","doi-asserted-by":"publisher","first-page":"103081","DOI":"10.1016\/j.isci.2021.103081","volume":"24","author":"Y Zhang","year":"2021","unstructured":"Zhang Y, Fan G, Liu X, Skovgaard K, Sturek M, Heegaard PMH (2021) The genome of the naturally evolved obesity-prone Ossabaw miniature pig. iScience 24:103081. https:\/\/doi.org\/10.1016\/j.isci.2021.103081","journal-title":"iScience"},{"key":"1112_CR71","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1161\/hc3201.094226","volume":"104","author":"MR Zile","year":"2001","unstructured":"Zile MR, Gaasch WH, Carroll JD, Feldman MD, Aurigemma GP, Schaer GL, Ghali JK, Liebson PR (2001) Heart failure with a normal ejection fraction: Is measurement of diastolic function necessary to make the diagnosis of diastolic heart failure? Circulation 104:779\u2013782. https:\/\/doi.org\/10.1161\/hc3201.094226","journal-title":"Circulation"},{"key":"1112_CR72","doi-asserted-by":"publisher","DOI":"10.1161\/circheartfailure.118.004969","volume":"11","author":"A Zweerink","year":"2018","unstructured":"Zweerink A, van der Lingen ACJ, Handoko ML, van Rossum AC, Allaart CP (2018) Chronotropic incompetence in chronic heart failure. Circ Heart Fail 11:e004969. https:\/\/doi.org\/10.1161\/circheartfailure.118.004969","journal-title":"Circ Heart Fail"}],"container-title":["Basic Research in Cardiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00395-025-01112-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00395-025-01112-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00395-025-01112-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T08:46:57Z","timestamp":1749631617000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00395-025-01112-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,1]]},"references-count":72,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["1112"],"URL":"https:\/\/doi.org\/10.1007\/s00395-025-01112-1","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.04.29.651284","asserted-by":"object"}]},"ISSN":["1435-1803"],"issn-type":[{"value":"1435-1803","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,1]]},"assertion":[{"value":"31 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 April 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 April 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 May 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}