{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:10:42Z","timestamp":1792782642514,"version":"4.5.0"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003125","name":"Suomen Kulttuurirahasto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005633","name":"Syd\u00e4ntutkimuss\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010133","name":"Aarne Koskelon S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010133","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Finnish Foundation for Laboratory Medicine"},{"DOI":"10.13039\/100010135","name":"Finska L\u00e4kares\u00e4llskapet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010135","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010116","name":"Medicinska Underst\u00f6dsf\u00f6reningen Liv och H\u00e4lsa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010116","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006306","name":"Sigrid Jus\u00e9liuksen S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006306","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Finnish state funding for university-level research"},{"name":"University of Helsinki","id":[{"id":"https:\/\/ror.org\/040af2s02","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Basic Res Cardiol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In contrast to humans, zebrafish hearts after cryoinjury undergo transient fibrotic scarring that subsequently resolves and renews with functional cardiomyocytes. To understand the molecular mechanisms underlying cardiac scarring and fibrotic resolution in zebrafish, we investigated cardiac transcriptomic responses in zebrafish at 14 days post-cryoinjury. Principal component analyses revealed distinct transcriptome clusters corresponding to healthy and injured hearts, highlighting significant changes during cardiac regeneration. Gene set enrichment analyses indicated that extracellular matrix organization and inflammatory response pathways were activated in cryoinjured hearts, while mitochondrial organization and oxidative phosphorylation pathways were downregulated. Notably, miR-145-5p was downregulated and\n                    <jats:italic>col5a1<\/jats:italic>\n                    upregulated in the cryoinjured hearts, contrasting with the expression patterns of miR-145-5p and COL5\u03b11 in the left ventricles of ischemic cardiomyopathy patients. Prediction of miRNA-mRNA interaction and a dual luciferase assay identified\n                    <jats:italic>COL5A1<\/jats:italic>\n                    as a target of miR-145-5p. Inhibition of miR-145-5p in human cardiac fibroblasts increased COL5A1 and \u03b1-SMA, decreased COL1A, and stimulated fibroblast differentiation, proliferation, and migration. Conversely, overexpression of miR-145-5p led to the opposite effects. Downregulation of COL5A1 suppressed fibroblast proliferation and migration, which could be rescued by inhibition of miR-145-5p. Moreover, downregulation of\n                    <jats:italic>col5a1<\/jats:italic>\n                    attenuated cardiac fibrotic scar resolution in zebrafish. Interestingly, downregulation of COL5A1 or inhibition of miR-145-5p diminished the expression of integrin subunits ITG\u03b23 and ITG\u03b25. Furthermore, inhibition of miR-145-5p potentiated the suppression of TGF-\u03b2\/SMAD signalling and the enhancement of fibroblast proliferation mediated by inhibiting integrin \u03b1v\u03b23 and \u03b1v\u03b25 with cilengitide. Collectively, miR-145-5p modulates collagen production and fibroblast behaviour partially via targeting COL5A1 and through integrin-mediated pathways.\n                  <\/jats:p>","DOI":"10.1007\/s00395-026-01199-0","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:58:52Z","timestamp":1785783532000},"page":"965-986","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MiR-145-5p modulates collagen production and fibroblast behaviour after cardiac injury partially via COL5A1"],"prefix":"10.1007","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5700-6786","authenticated-orcid":false,"given":"Hong","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jere","family":"Paavola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanni","family":"Perttunen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katariina","family":"Immonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"H\u00e4gerstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suneeta","family":"Narumanchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karri","family":"Kalervo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Tolva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juha","family":"Sinisalo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikko I.","family":"M\u00e4yr\u00e4np\u00e4\u00e4","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heikki","family":"Ruskoaho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riikka","family":"Kosonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vesa M.","family":"Olkkonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Laine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilkka","family":"Tikkanen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e4ivi","family":"Lakkisto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"1199_CR1","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1038\/s41569-019-0315-x","volume":"17","author":"L Adamo","year":"2020","unstructured":"Adamo L, Rocha-Resende C, Prabhu SD, Mann DL (2020) Reappraising the role of inflammation in heart failure. Nat Rev Cardiol 17:269\u2013285. https:\/\/doi.org\/10.1038\/s41569-019-0315-x","journal-title":"Nat Rev Cardiol"},{"key":"1199_CR2","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.1172\/JCI72181","volume":"124","author":"AB Aurora","year":"2014","unstructured":"Aurora AB, Porrello ER, Tan W, Mahmoud AI, Hill JA, Bassel-Duby R, Sadek HA, Olson EN (2014) Macrophages are required for neonatal heart regeneration. J Clin Invest 124:1382\u20131392. https:\/\/doi.org\/10.1172\/JCI72181","journal-title":"J Clin Invest"},{"key":"1199_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2022.101310","volume":"31","author":"SS Baksh","year":"2022","unstructured":"Baksh SS, Hodgkinson CP (2022) Conservation of miR combo based direct cardiac reprogramming. Biochem Biophys Rep 31:101310. https:\/\/doi.org\/10.1016\/j.bbrep.2022.101310","journal-title":"Biochem Biophys Rep"},{"key":"1199_CR4","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202004198","volume":"219","author":"M Benito-Jard\u00f3n","year":"2020","unstructured":"Benito-Jard\u00f3n M, Strohmeyer N, Ortega-Sanch\u00eds S, Bharadwaj M, Moser M, M\u00fcller DJ, F\u00e4ssler R, Costell M (2020) \u0391v-class integrin binding to fibronectin is solely mediated by RGD and unaffected by an RGE mutation. J Cell Biol 219:e202004198. https:\/\/doi.org\/10.1083\/jcb.202004198","journal-title":"J Cell Biol"},{"key":"1199_CR5","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/S0968-4328(00)00043-3","volume":"32","author":"DE Birk","year":"2001","unstructured":"Birk DE (2001) Type V collagen: heterotypic type I\/V collagen interactions in the regulation of fibril assembly. Micron 32:223\u2013237. https:\/\/doi.org\/10.1016\/S0968-4328(00)00043-3","journal-title":"Micron"},{"key":"1199_CR6","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","volume":"30","author":"AM Bolger","year":"2014","unstructured":"Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114\u20132120. https:\/\/doi.org\/10.1093\/bioinformatics\/btu170","journal-title":"Bioinformatics"},{"key":"1199_CR7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-68223-8","volume":"10","author":"M Bouvet","year":"2020","unstructured":"Bouvet M, Claude O, Roux M, Skelly D, Masurkar N, Mougenot N, Nadaud S, Blanc C, Delacroix C, Chardonnet S, Pionneau C, Perret C, Yaniz-Galende E, Rosenthal N, Tr\u00e9gou\u00ebt D-A, Marazzi G, Silvestre J-S, Sassoon D, Hulot J-S (2020) Anti-integrin \u03b1v therapy improves cardiac fibrosis after myocardial infarction by blunting cardiac PW1+ stromal cells. Sci Rep 10:11404. https:\/\/doi.org\/10.1038\/s41598-020-68223-8","journal-title":"Sci Rep"},{"key":"1199_CR8","doi-asserted-by":"publisher","first-page":"2841","DOI":"10.1172\/JCI98890","volume":"129","author":"MD Burkhalter","year":"2019","unstructured":"Burkhalter MD, Sridhar A, Sampaio P, Jacinto R, Burczyk MS, Donow C, Angenendt M, Competence Network for Congenital Heart Defects Investigators, Hempel M, Walther P, Pennekamp P, Omran H, Lopes SS, Ware SM, Philipp M (2019) Imbalanced mitochondrial function provokes heterotaxy via aberrant ciliogenesis. J Clin Invest 129:2841\u20132855. https:\/\/doi.org\/10.1172\/JCI98890","journal-title":"J Clin Invest"},{"key":"1199_CR9","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","volume":"29","author":"A Dobin","year":"2013","unstructured":"Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR (2013) STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29:15\u201321. https:\/\/doi.org\/10.1093\/bioinformatics\/bts635","journal-title":"Bioinformatics"},{"key":"1199_CR10","doi-asserted-by":"publisher","first-page":"85","DOI":"10.3109\/00365513.2014.981855","volume":"75","author":"Y-M Dong","year":"2015","unstructured":"Dong Y-M, Liu X-X, Wei G-Q, Da Y-N, Cha L, Ma C-S (2015) Prediction of long-term outcome after acute myocardial infarction using circulating miR-145. Scand J Clin Lab Invest 75:85\u201391. https:\/\/doi.org\/10.3109\/00365513.2014.981855","journal-title":"Scand J Clin Lab Invest"},{"key":"1199_CR11","doi-asserted-by":"publisher","first-page":"1184","DOI":"10.1038\/nprot.2009.97","volume":"4","author":"S Durinck","year":"2009","unstructured":"Durinck S, Spellman PT, Birney E, Huber W (2009) Mapping identifiers for the integration of genomic datasets with the R\/Bioconductor package biomaRt. Nat Protoc 4:1184\u20131191. https:\/\/doi.org\/10.1038\/nprot.2009.97","journal-title":"Nat Protoc"},{"key":"1199_CR12","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1038\/nature11739","volume":"492","author":"A Eulalio","year":"2012","unstructured":"Eulalio A, Mano M, Ferro MD, Zentilin L, Sinagra G, Zacchigna S, Giacca M (2012) Functional screening identifies miRNAs inducing cardiac regeneration. Nature 492:376\u2013381. https:\/\/doi.org\/10.1038\/nature11739","journal-title":"Nature"},{"key":"1199_CR13","doi-asserted-by":"publisher","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","volume":"32","author":"P Ewels","year":"2016","unstructured":"Ewels P, Magnusson M, Lundin S, K\u00e4ller M (2016) MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics 32:3047\u20133048. https:\/\/doi.org\/10.1093\/bioinformatics\/btw354","journal-title":"Bioinformatics"},{"key":"1199_CR14","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/nrcardio.2014.28","volume":"11","author":"NG Frangogiannis","year":"2014","unstructured":"Frangogiannis NG (2014) The inflammatory response in myocardial injury, repair, and remodelling. Nat Rev Cardiol 11:255\u2013265. https:\/\/doi.org\/10.1038\/nrcardio.2014.28","journal-title":"Nat Rev Cardiol"},{"key":"1199_CR15","doi-asserted-by":"publisher","first-page":"1600","DOI":"10.1172\/JCI87491","volume":"127","author":"NG Frangogiannis","year":"2017","unstructured":"Frangogiannis NG (2017) The extracellular matrix in myocardial injury, repair, and remodeling. J Clin Invest 127:1600\u20131612. https:\/\/doi.org\/10.1172\/JCI87491","journal-title":"J Clin Invest"},{"key":"1199_CR16","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201949752","volume":"21","author":"R Fukuda","year":"2020","unstructured":"Fukuda R, Mar\u00edn-Juez R, El-Sammak H, Beisaw A, Ramadass R, Kuenne C, Guenther S, Konzer A, Bhagwat AM, Graumann J, Stainier DY (2020) Stimulation of glycolysis promotes cardiomyocyte proliferation after injury in adult zebrafish. EMBO Rep 21:e49752. https:\/\/doi.org\/10.15252\/embr.201949752","journal-title":"EMBO Rep"},{"key":"1199_CR17","doi-asserted-by":"publisher","first-page":"2","DOI":"10.3390\/jcdd4010002","volume":"4","author":"L Gamba","year":"2017","unstructured":"Gamba L, Amin-Javaheri A, Kim J, Warburton D, Lien C-L (2017) Collagenolytic Activity Is Associated with Scar Resolution in Zebrafish Hearts after Cryoinjury. J Cardiovasc Dev Dis 4:2. https:\/\/doi.org\/10.3390\/jcdd4010002","journal-title":"J Cardiovasc Dev Dis"},{"key":"1199_CR18","doi-asserted-by":"publisher","first-page":"1745","DOI":"10.1074\/mcp.RA118.001193","volume":"18","author":"A Garcia-Puig","year":"2019","unstructured":"Garcia-Puig A, Mosquera JL, Jim\u00e9nez-Delgado S, Garc\u00eda-Pastor C, Jorba I, Navajas D, Canals F, Raya A (2019) Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration*[S]. Mol Cell Proteomics 18:1745\u20131755. https:\/\/doi.org\/10.1074\/mcp.RA118.001193","journal-title":"Mol Cell Proteomics"},{"key":"1199_CR19","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1002\/reg2.83","volume":"4","author":"JM Gonzalez-Rosa","year":"2017","unstructured":"Gonzalez-Rosa JM, Burns CE, Burns CG (2017) Zebrafish heart regeneration: 15 years of discoveries. Regeneration (Oxford) 4:105\u2013123. https:\/\/doi.org\/10.1002\/reg2.83","journal-title":"Regeneration (Oxford)"},{"key":"1199_CR20","doi-asserted-by":"publisher","first-page":"1663","DOI":"10.1242\/dev.060897","volume":"138","author":"JM Gonz\u00e1lez-Rosa","year":"2011","unstructured":"Gonz\u00e1lez-Rosa JM, Mart\u00edn V, Peralta M, Torres M, Mercader N (2011) Extensive scar formation and regression during heart regeneration after cryoinjury in zebrafish. Development 138:1663\u20131674. https:\/\/doi.org\/10.1242\/dev.060897","journal-title":"Development"},{"key":"1199_CR21","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1038\/s41569-018-0036-6","volume":"15","author":"H Hashimoto","year":"2018","unstructured":"Hashimoto H, Olson EN, Bassel-Duby R (2018) Therapeutic approaches for cardiac regeneration and repair. Nat Rev Cardiol 15:585\u2013600. https:\/\/doi.org\/10.1038\/s41569-018-0036-6","journal-title":"Nat Rev Cardiol"},{"key":"1199_CR22","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1186\/1471-2407-14-108","volume":"14","author":"M Hayashi","year":"2014","unstructured":"Hayashi M, Nomoto S, Hishida M, Inokawa Y, Kanda M, Okamura Y, Nishikawa Y, Tanaka C, Kobayashi D, Yamada S, Nakayama G, Fujii T, Sugimoto H, Koike M, Fujiwara M, Takeda S, Kodera Y (2014) Identification of the collagen type 1 \u03b1 1 gene (COL1A1) as a candidate survival-related factor associated with hepatocellular carcinoma. BMC Cancer 14:108. https:\/\/doi.org\/10.1186\/1471-2407-14-108","journal-title":"BMC Cancer"},{"key":"1199_CR23","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50163","volume":"8","author":"H Honkoop","year":"2019","unstructured":"Honkoop H, de Bakker DE, Aharonov A, Kruse F, Shakked A, Nguyen PD, de Heus C, Garric L, Muraro MJ, Shoffner A, Tessadori F, Peterson JC, Noort W, Bertozzi A, Weidinger G, Posthuma G, Gr\u00fcn D, van der Laarse WJ, Klumperman J, Jaspers RT, Poss KD, van Oudenaarden A, Tzahor E, Bakkers J (2019) Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart. Elife 8:e50163. https:\/\/doi.org\/10.7554\/eLife.50163","journal-title":"Elife"},{"key":"1199_CR24","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/j.jacbts.2019.02.006","volume":"4","author":"C Humeres","year":"2019","unstructured":"Humeres C, Frangogiannis NG (2019) Fibroblasts in the infarcted, remodeling, and failing heart. JACC Basic Transl Sci 4:449\u2013467. https:\/\/doi.org\/10.1016\/j.jacbts.2019.02.006","journal-title":"JACC Basic Transl Sci"},{"key":"1199_CR25","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1038\/nature08899","volume":"464","author":"C Jopling","year":"2010","unstructured":"Jopling C, Sleep E, Raya M, Mart\u00ed M, Raya A, Izpis\u00faa Belmonte JC (2010) Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation. Nature 464:606\u2013609. https:\/\/doi.org\/10.1038\/nature08899","journal-title":"Nature"},{"key":"1199_CR26","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1530\/JOE-10-0377","volume":"209","author":"S-H Kim","year":"2011","unstructured":"Kim S-H, Turnbull J, Guimond S (2011) Extracellular matrix and cell signalling: the dynamic cooperation of integrin, proteoglycan and growth factor receptor. J Endocrinol 209:139\u2013151. https:\/\/doi.org\/10.1530\/JOE-10-0377","journal-title":"J Endocrinol"},{"key":"1199_CR27","doi-asserted-by":"publisher","DOI":"10.3390\/biom9010011","volume":"9","author":"H Klett","year":"2019","unstructured":"Klett H, J\u00fcrgensen L, Most P, Busch M, G\u00fcnther F, Dobreva G, Leuschner F, Hassel D, Busch H, Boerries M (2019) Delineating the dynamic transcriptome response of mRNA and microRNA during zebrafish heart regeneration. Biomolecules 9:11. https:\/\/doi.org\/10.3390\/biom9010011","journal-title":"Biomolecules"},{"key":"1199_CR28","doi-asserted-by":"publisher","first-page":"D68","DOI":"10.1093\/nar\/gkt1181","volume":"42","author":"A Kozomara","year":"2014","unstructured":"Kozomara A, Griffiths-Jones S (2014) miRBase: annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Res 42:D68\u2013D73. https:\/\/doi.org\/10.1093\/nar\/gkt1181","journal-title":"Nucleic Acids Res"},{"key":"1199_CR29","doi-asserted-by":"publisher","first-page":"16029","DOI":"10.1073\/pnas.1406508111","volume":"111","author":"KJ Lavine","year":"2014","unstructured":"Lavine KJ, Epelman S, Uchida K, Weber KJ, Nichols CG, Schilling JD, Ornitz DM, Randolph GJ, Mann DL (2014) Distinct macrophage lineages contribute to disparate patterns of cardiac recovery and remodeling in the neonatal and adult heart. Proc Natl Acad Sci U S A 111:16029\u201316034. https:\/\/doi.org\/10.1073\/pnas.1406508111","journal-title":"Proc Natl Acad Sci U S A"},{"key":"1199_CR30","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","volume":"30","author":"Y Liao","year":"2014","unstructured":"Liao Y, Smyth GK, Shi W (2014) FeatureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics 30:923\u2013930. https:\/\/doi.org\/10.1093\/bioinformatics\/btt656","journal-title":"Bioinformatics"},{"key":"1199_CR31","doi-asserted-by":"publisher","first-page":"H1274","DOI":"10.1152\/ajpheart.1990.258.5.H1274","volume":"258","author":"GD Lopaschuk","year":"1990","unstructured":"Lopaschuk GD, Spafford MA (1990) Energy substrate utilization by isolated working hearts from newborn rabbits. Am J Physiol 258:H1274-1280. https:\/\/doi.org\/10.1152\/ajpheart.1990.258.5.H1274","journal-title":"Am J Physiol"},{"key":"1199_CR32","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15:550. https:\/\/doi.org\/10.1186\/s13059-014-0550-8","journal-title":"Genome Biol"},{"key":"1199_CR33","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1164\/rccm.201704-0732OC","volume":"197","author":"F Lutful Kabir","year":"2018","unstructured":"Lutful Kabir F, Ambalavanan N, Liu G, Li P, Solomon GM, Lal CV, Mazur M, Halloran B, Szul T, Gerthoffer WT, Rowe SM, Harris WT (2018) MicroRNA-145 antagonism reverses TGF-\u03b2 inhibition of F508del CFTR correction in airway epithelia. Am J Respir Crit Care Med 197:632\u2013643. https:\/\/doi.org\/10.1164\/rccm.201704-0732OC","journal-title":"Am J Respir Crit Care Med"},{"key":"1199_CR34","doi-asserted-by":"publisher","first-page":"753","DOI":"10.2174\/187152010794728639","volume":"10","author":"C Mas-Moruno","year":"2010","unstructured":"Mas-Moruno C, Rechenmacher F, Kessler H (2010) Cilengitide: the first anti-angiogenic small molecule drug candidate design, synthesis and clinical evaluation. Anticancer Agents Med Chem 10:753\u2013768. https:\/\/doi.org\/10.2174\/187152010794728639","journal-title":"Anticancer Agents Med Chem"},{"key":"1199_CR35","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/s00395-010-0123-2","volume":"106","author":"B Meder","year":"2011","unstructured":"Meder B, Keller A, Vogel B, Haas J, Sedaghat-Hamedani F, Kayvanpour E, Just S, Borries A, Rudloff J, Leidinger P, Meese E, Katus HA, Rottbauer W (2011) MicroRNA signatures in total peripheral blood as novel biomarkers for acute myocardial infarction. Basic Res Cardiol 106:13\u201323. https:\/\/doi.org\/10.1007\/s00395-010-0123-2","journal-title":"Basic Res Cardiol"},{"key":"1199_CR36","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd6020016","volume":"6","author":"S Narumanchi","year":"2019","unstructured":"Narumanchi S, Kalervo K, Perttunen S, Wang H, Immonen K, Kosonen R, Laine M, Ruskoaho H, Tikkanen I, Lakkisto P, Paavola J (2019) Inhibition of let-7c regulates cardiac regeneration after cryoinjury in adult zebrafish. J Cardiovasc Dev Dis 6:16. https:\/\/doi.org\/10.3390\/jcdd6020016","journal-title":"J Cardiovasc Dev Dis"},{"key":"1199_CR37","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1093\/bioinformatics\/btv566","volume":"32","author":"K Okonechnikov","year":"2016","unstructured":"Okonechnikov K, Conesa A, Garc\u00eda-Alcalde F (2016) Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data. Bioinformatics 32:292\u2013294. https:\/\/doi.org\/10.1093\/bioinformatics\/btv566","journal-title":"Bioinformatics"},{"key":"1199_CR38","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1186\/s12967-018-1730-1","volume":"16","author":"GL Perrucci","year":"2018","unstructured":"Perrucci GL, Barbagallo VA, Corlian\u00f2 M, Tosi D, Santoro R, Nigro P, Poggio P, Bulfamante G, Lombardi F, Pompilio G (2018) Integrin \u03b1\u03bd\u03b25 in vitro inhibition limits pro-fibrotic response in cardiac fibroblasts of spontaneously hypertensive rats. J Transl Med 16:352. https:\/\/doi.org\/10.1186\/s12967-018-1730-1","journal-title":"J Transl Med"},{"key":"1199_CR39","doi-asserted-by":"publisher","first-page":"2188","DOI":"10.1126\/science.1077857","volume":"298","author":"KD Poss","year":"2002","unstructured":"Poss KD, Wilson LG, Keating MT (2002) Heart regeneration in zebrafish. Science 298:2188\u20132190. https:\/\/doi.org\/10.1126\/science.1077857","journal-title":"Science"},{"key":"1199_CR40","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/j.cell.2014.03.032","volume":"157","author":"BN Puente","year":"2014","unstructured":"Puente BN, Kimura W, Muralidhar SA, Moon J, Amatruda JF, Phelps KL, Grinsfelder D, Rothermel BA, Chen R, Garcia JA, Santos CX, Thet S, Mori E, Kinter MT, Rindler PM, Zacchigna S, Mukherjee S, Chen DJ, Mahmoud AI, Giacca M, Rabinovitch PS, Aroumougame A, Shah AM, Szweda LI, Sadek HA (2014) The oxygen-rich postnatal environment induces cardiomyocyte cell-cycle arrest through DNA damage response. Cell 157:565\u2013579. https:\/\/doi.org\/10.1016\/j.cell.2014.03.032","journal-title":"Cell"},{"key":"1199_CR41","doi-asserted-by":"publisher","first-page":"1629","DOI":"10.1042\/BST20140263","volume":"42","author":"J Saif","year":"2014","unstructured":"Saif J, Emanueli C (2014) MiRNAs in post-ischaemic angiogenesis and vascular remodelling. Biochem Soc Trans 42:1629\u20131636. https:\/\/doi.org\/10.1042\/BST20140263","journal-title":"Biochem Soc Trans"},{"key":"1199_CR42","doi-asserted-by":"publisher","first-page":"4188","DOI":"10.1073\/pnas.1716713115","volume":"115","author":"H S\u00e1nchez-Iranzo","year":"2018","unstructured":"S\u00e1nchez-Iranzo H, Galardi-Castilla M, Sanz-Morej\u00f3n A, Gonz\u00e1lez-Rosa JM, Costa R, Ernst A, Sainz de Aja J, Langa X, Mercader N (2018) Transient fibrosis resolves via fibroblast inactivation in the regenerating zebrafish heart. Proc Natl Acad Sci U S A 115:4188\u20134193. https:\/\/doi.org\/10.1073\/pnas.1716713115","journal-title":"Proc Natl Acad Sci U S A"},{"key":"1199_CR43","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.gde.2020.05.020","volume":"64","author":"A Sanz-Morej\u00f3n","year":"2020","unstructured":"Sanz-Morej\u00f3n A, Mercader N (2020) Recent insights into zebrafish cardiac regeneration. Curr Opin Genet Dev 64:37\u201343. https:\/\/doi.org\/10.1016\/j.gde.2020.05.020","journal-title":"Curr Opin Genet Dev"},{"key":"1199_CR44","doi-asserted-by":"publisher","first-page":"4287","DOI":"10.1242\/jcs.113.23.4287","volume":"113","author":"J Sottile","year":"2000","unstructured":"Sottile J, Hocking DC, Langenbach KJ (2000) Fibronectin polymerization stimulates cell growth by RGD-dependent and-independent mechanisms. J Cell Sci 113:4287\u20134299. https:\/\/doi.org\/10.1242\/jcs.113.23.4287","journal-title":"J Cell Sci"},{"key":"1199_CR45","doi-asserted-by":"publisher","first-page":"15545","DOI":"10.1073\/pnas.0506580102","volume":"102","author":"A Subramanian","year":"2005","unstructured":"Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, Paulovich A, Pomeroy SL, Golub TR, Lander ES, Mesirov JP (2005) Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci U S A 102:15545\u201315550. https:\/\/doi.org\/10.1073\/pnas.0506580102","journal-title":"Proc Natl Acad Sci U S A"},{"key":"1199_CR46","doi-asserted-by":"publisher","first-page":"4096","DOI":"10.1242\/jcs.091363","volume":"124","author":"M Sun","year":"2011","unstructured":"Sun M, Chen S, Adams SM, Florer JB, Liu H, Kao WW-Y, Wenstrup RJ, Birk DE (2011) Collagen V is a dominant regulator of collagen fibrillogenesis: dysfunctional regulation of structure and function in a corneal-stroma-specific Col5a1-null mouse model. J Cell Sci 124:4096\u20134105. https:\/\/doi.org\/10.1242\/jcs.091363","journal-title":"J Cell Sci"},{"key":"1199_CR47","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1186\/s12864-018-5213-9","volume":"19","author":"ME Sweet","year":"2018","unstructured":"Sweet ME, Cocciolo A, Slavov D, Jones KL, Sweet JR, Graw SL, Reece TB, Ambardekar AV, Bristow MR, Mestroni L, Taylor MRG (2018) Transcriptome analysis of human heart failure reveals dysregulated cell adhesion in dilated cardiomyopathy and activated immune pathways in ischemic heart failure. BMC Genomics 19:812. https:\/\/doi.org\/10.1186\/s12864-018-5213-9","journal-title":"BMC Genomics"},{"key":"1199_CR48","doi-asserted-by":"publisher","first-page":"F121","DOI":"10.1152\/ajprenal.00313.2022","volume":"325","author":"A Tabei","year":"2023","unstructured":"Tabei A, Sakairi T, Hamatani H, Ohishi Y, Watanabe M, Nakasatomi M, Ikeuchi H, Kaneko Y, Kopp JB, Hiromura K (2023) The miR-143\/145 cluster induced by TGF-\u03b21 suppresses Wilms\u2019 tumor 1 expression in cultured human podocytes. Am J Physiol Renal Physiol 325:F121\u2013F133. https:\/\/doi.org\/10.1152\/ajprenal.00313.2022","journal-title":"Am J Physiol Renal Physiol"},{"key":"1199_CR49","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1007\/s00441-016-2431-9","volume":"365","author":"V Talman","year":"2016","unstructured":"Talman V, Ruskoaho H (2016) Cardiac fibrosis in myocardial infarction\u2014from repair and remodeling to regeneration. Cell Tissue Res 365:563\u2013581. https:\/\/doi.org\/10.1007\/s00441-016-2431-9","journal-title":"Cell Tissue Res"},{"key":"1199_CR50","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.919355","volume":"9","author":"C Wang","year":"2022","unstructured":"Wang C, Taskinen JH, Segersv\u00e4rd H, Immonen K, Kosonen R, Tolva JM, M\u00e4yr\u00e4np\u00e4\u00e4 MI, Kovanen PT, Olkkonen VM, Sinisalo J, Laine M, Tikkanen I, Lakkisto P (2022) Alterations of cardiac protein kinases in cyclic nucleotide-dependent signaling pathways in human ischemic heart failure. Front Cardiovasc Med 9:919355. https:\/\/doi.org\/10.3389\/fcvm.2022.919355","journal-title":"Front Cardiovasc Med"},{"key":"1199_CR51","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231710059","volume":"23","author":"H Wang","year":"2022","unstructured":"Wang H, Segersv\u00e4rd H, Siren J, Perttunen S, Immonen K, Kosonen R, Chen Y-C, Tolva J, Laivuori M, M\u00e4yr\u00e4np\u00e4\u00e4 MI, Kovanen PT, Sinisalo J, Laine M, Tikkanen I, Lakkisto P (2022) Tankyrase inhibition attenuates cardiac dilatation and dysfunction in ischemic heart failure. Int J Mol Sci 23:10059. https:\/\/doi.org\/10.3390\/ijms231710059","journal-title":"Int J Mol Sci"},{"key":"1199_CR52","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.18243","volume":"28","author":"H Wang","year":"2024","unstructured":"Wang H, Siren J, Perttunen S, Immonen K, Chen Y-C, Narumanchi S, Kosonen R, Paavola J, Laine M, Tikkanen I, Lakkisto P (2024) Deficiency of heme oxygenase 1a causes detrimental effects on cardiac function. J Cell Mol Med 28:e18243. https:\/\/doi.org\/10.1111\/jcmm.18243","journal-title":"J Cell Mol Med"},{"key":"1199_CR53","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1242\/dmm.026989","volume":"10","author":"LW Wang","year":"2017","unstructured":"Wang LW, Huttner IG, Santiago CF, Kesteven SH, Yu Z-Y, Feneley MP, Fatkin D (2017) Standardized echocardiographic assessment of cardiac function in normal adult zebrafish and heart disease models. Dis Model Mech 10:63\u201376. https:\/\/doi.org\/10.1242\/dmm.026989","journal-title":"Dis Model Mech"},{"key":"1199_CR54","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.yjmcc.2013.08.007","volume":"66","author":"Y-S Wang","year":"2014","unstructured":"Wang Y-S, Li S-H, Guo J, Mihic A, Wu J, Sun L, Davis K, Weisel RD, Li R-K (2014) Role of miR-145 in cardiac myofibroblast differentiation. J Mol Cell Cardiol 66:94\u2013105. https:\/\/doi.org\/10.1016\/j.yjmcc.2013.08.007","journal-title":"J Mol Cell Cardiol"},{"key":"1199_CR55","doi-asserted-by":"publisher","first-page":"53331","DOI":"10.1074\/jbc.M409622200","volume":"279","author":"RJ Wenstrup","year":"2004","unstructured":"Wenstrup RJ, Florer JB, Brunskill EW, Bell SM, Chervoneva I, Birk DE (2004) Type V collagen controls the initiation of collagen fibril assembly. J Biol Chem 279:53331\u201353337. https:\/\/doi.org\/10.1074\/jbc.M409622200","journal-title":"J Biol Chem"},{"key":"1199_CR56","doi-asserted-by":"publisher","first-page":"D710","DOI":"10.1093\/nar\/gkv1157","volume":"44","author":"A Yates","year":"2016","unstructured":"Yates A, Akanni W, Amode MR, Barrell D, Billis K, Carvalho-Silva D, Cummins C, Clapham P, Fitzgerald S, Gil L, Gir\u00f3n CG, Gordon L, Hourlier T, Hunt SE, Janacek SH, Johnson N, Juettemann T, Keenan S, Lavidas I, Martin FJ, Maurel T, McLaren W, Murphy DN, Nag R, Nuhn M, Parker A, Patricio M, Pignatelli M, Rahtz M, Riat HS, Sheppard D, Taylor K, Thormann A, Vullo A, Wilder SP, Zadissa A, Birney E, Harrow J, Muffato M, Perry E, Ruffier M, Spudich G, Trevanion SJ, Cunningham F, Aken BL, Zerbino DR, Flicek P (2016) Ensembl 2016. Nucleic Acids Res 44:D710\u2013D716. https:\/\/doi.org\/10.1093\/nar\/gkv1157","journal-title":"Nucleic Acids Res"},{"key":"1199_CR57","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/j.cell.2020.06.030","volume":"182","author":"T Yokota","year":"2020","unstructured":"Yokota T, McCourt J, Ma F, Ren S, Li S, Kim T-H, Kurmangaliyev YZ, Nasiri R, Ahadian S, Nguyen T, Tan XHM, Zhou Y, Wu R, Rodriguez A, Cohn W, Wang Y, Whitelegge J, Ryazantsev S, Khademhosseini A, Teitell MA, Chiou P-Y, Birk DE, Rowat AC, Crosbie RH, Pellegrini M, Seldin M, Lusis AJ, Deb A (2020) Type V collagen in scar tissue regulates the size of scar after heart injury. Cell 182:545-562.e23. https:\/\/doi.org\/10.1016\/j.cell.2020.06.030","journal-title":"Cell"},{"key":"1199_CR58","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1093\/cvr\/cvu057","volume":"102","author":"S Zacchigna","year":"2014","unstructured":"Zacchigna S, Giacca M (2014) Extra- and intracellular factors regulating cardiomyocyte proliferation in postnatal life. Cardiovasc Res 102:312\u2013320. https:\/\/doi.org\/10.1093\/cvr\/cvu057","journal-title":"Cardiovasc Res"},{"key":"1199_CR59","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1016\/j.bj.2021.11.012","volume":"45","author":"W Zhou","year":"2022","unstructured":"Zhou W, Ji L, Liu X, Tu D, Shi N, Yangqu W, Chen S, Gao P, Zhu H, Ruan C (2022) AIFM1, negatively regulated by miR-145-5p, aggravates hypoxia-induced cardiomyocyte injury. Biomed J 45:870\u2013882. https:\/\/doi.org\/10.1016\/j.bj.2021.11.012","journal-title":"Biomed J"}],"container-title":["Basic Research in Cardiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00395-026-01199-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00395-026-01199-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00395-026-01199-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:46:32Z","timestamp":1789994792000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00395-026-01199-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,3]]},"references-count":59,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["1199"],"URL":"https:\/\/doi.org\/10.1007\/s00395-026-01199-0","relation":{},"ISSN":["1435-1803"],"issn-type":[{"value":"1435-1803","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,3]]},"assertion":[{"value":"8 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 June 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 July 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 August 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that there is no conflict of interest associated with this manuscript.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Zebrafish experiments were approved by the Regional State Administrative Agency for Southern Finland in agreement with the ethical guidelines of the European convention (ESAVI\/2988\/04.https:\/\/doi.org\/10.07\/2014; ESAVI\/4131\/04. https:\/\/doi.org\/10.07\/2017\n; ESAVI\/16286\/2020).The investigation of human samples was conducted in accordance with the Declaration of Helsinki, and the protocol was approved by the Ethics Committee of Helsinki and Uusimaa Hospital District, Finland. Written informed consent was obtained from all patients. The National Authority for Medicolegal Affairs approved the usage of tissues from organ donors.","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}]}}