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Rodent models have yet to produce clinical therapies, and the exploration of larger and more diverse models remains relatively scarce. We investigated the potential for brain injury after headbutting in two combative bovid species by assessing neuromorphology and neuropathology through immunohistochemistry and stereological quantification. Postmortem brains of muskoxen (<jats:italic>Ovibos moschatus<\/jats:italic>,<jats:italic>n<\/jats:italic>\u2009=\u20093) and bighorn sheep (<jats:italic>Ovis canadensis<\/jats:italic>,<jats:italic>n<\/jats:italic>\u2009=\u20094) were analyzed by high-resolution MRI and processed histologically for evidence of TBI. Exploratory histological protocols investigated potential abnormalities in neurons, microglia, and astrocytes in the prefrontal and parietal cortex. Phosphorylated tau protein, a TBI biomarker found in the cerebrospinal fluid and in neurodegenerative lesions, was used to detect possible cellular consequences of chronic or acute TBI. MRI revealed no abnormal neuropathological changes; however, high amounts of tau-immunoreactive neuritic thread clusters, neurites, and neurons were concentrated in the superficial layers of the neocortex, preferentially at the bottom of the sulci in the muskoxen and occasionally around blood vessels. Tau-immunoreactive lesions were rare in the bighorn sheep. Additionally, microglia and astrocytes showed no grouping around tau-immunoreactive cells in either species. Our preliminary findings indicate that muskoxen and possibly other headbutting bovids suffer from chronic or acute brain trauma and that the males\u2019 thicker skulls may protect them to a certain extent.<\/jats:p>","DOI":"10.1007\/s00401-022-02427-2","type":"journal-article","created":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T10:09:07Z","timestamp":1652782147000},"page":"5-26","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Evidence of traumatic brain injury in headbutting bovids"],"prefix":"10.1007","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8336-1888","authenticated-orcid":false,"given":"Nicole L.","family":"Ackermans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1517-3903","authenticated-orcid":false,"given":"Merina","family":"Varghese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8170-009X","authenticated-orcid":false,"given":"Terrie M.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9085-3474","authenticated-orcid":false,"given":"Nicholas","family":"Grimaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5350-4566","authenticated-orcid":false,"given":"Enna","family":"Selmanovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7981-6808","authenticated-orcid":false,"given":"Akbar","family":"Alipour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7709-5835","authenticated-orcid":false,"given":"Priti","family":"Balchandani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4180-7156","authenticated-orcid":false,"given":"Joy S.","family":"Reidenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3208-1154","authenticated-orcid":false,"given":"Patrick R.","family":"Hof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,5,17]]},"reference":[{"key":"2427_CR1","unstructured":"Centers for Disease Control and Prevention (2019) Surveillance Report of Traumatic Brain Injury-related Emergency Department Visits, Hospitalizations, and Deaths\u2014United States, 2014., ed. 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