{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:01:16Z","timestamp":1791576076177,"version":"4.3.4"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Gynecol Obstet"],"published-print":{"date-parts":[[2021,4]]},"DOI":"10.1007\/s00404-020-05845-3","type":"journal-article","created":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T17:10:06Z","timestamp":1614100206000},"page":"1049-1056","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Quick assessment of DNA damage in cervical epithelial cells using a chromatin dispersion test"],"prefix":"10.1007","volume":"303","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5025-1284","authenticated-orcid":false,"given":"Elva I.","family":"Cort\u00e9s-Guti\u00e9rrez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha I.","family":"D\u00e1vila-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Homero","family":"S\u00e1nchez-D\u00e1vila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Garc\u00eda de laVega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo M.","family":"Cerda-Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Gos\u00e1lvez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,23]]},"reference":[{"issue":"1","key":"5845_CR1","first-page":"197","volume":"56","author":"MR Mullokandov","year":"1996","unstructured":"Mullokandov MR, Kholodilov NG, Atkin NB, Burk RD, Johnson AB, Klinger HP (1996) Genomic alterations in cervical carcinoma: losses of chromosome heterozygosity and human papilloma virus tumor status. Cancer Res 56(1):197\u2013205","journal-title":"Cancer Res"},{"issue":"2","key":"5845_CR2","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1093\/carcin\/bgi218","volume":"27","author":"AJ Olaharski","year":"2006","unstructured":"Olaharski AJ, Sotelo R, Solorza-Luna G, Eastmond DA (2006) Tetraploidy and chromosomal instability are early events during cervical carcinogenesis. Carcinogenesis 27(2):337\u2013343","journal-title":"Carcinogenesis"},{"issue":"1\u20132","key":"5845_CR3","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/S1383-5718(01)00348-5","volume":"515","author":"CH Leal-Garza","year":"2002","unstructured":"Leal-Garza CH, Cerda-Flores RM, Leal-Elizondo E, Cort\u00e9s-Guti\u00e9rrez EI (2002) Micronuclei in cervical smears and peripheral blood lymphocytes from women with and without cervical uterine cancer. Mutat Res 515(1\u20132):57\u201362","journal-title":"Mutat Res"},{"issue":"2","key":"5845_CR4","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/S0165-4608(00)00286-7","volume":"122","author":"EI Cort\u00e9s-Guti\u00e9rrez","year":"2000","unstructured":"Cort\u00e9s-Guti\u00e9rrez EI, Cerda-Flores RM, Leal-Garza CH (2000) Sister chromatid exchanges in peripheral lymphocytes from women with carcinoma of the uterine cervix. Cancer Genet Cytogenet 122(2):121\u2013123","journal-title":"Cancer Genet Cytogenet"},{"issue":"4","key":"5845_CR5","first-page":"207","volume":"32","author":"EI Cort\u00e9s-Guti\u00e9rrez","year":"2010","unstructured":"Cort\u00e9s-Guti\u00e9rrez EI, D\u00e1vila-Rodr\u00edguez MI, Zamudio-Gonz\u00e1lez EA, Aguado-Barrera ME, Vargas-Villarreal J, Cerda-Flores RM (2010) DNA damage in Mexican women with cervical dysplasia evaluated by comet assay. Anal Quant Cytol Histol 32(4):207\u2013213","journal-title":"Anal Quant Cytol Histol"},{"issue":"2","key":"5845_CR6","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/S1383-5718(97)00164-2","volume":"412","author":"A Udumudi","year":"1998","unstructured":"Udumudi A, Jaiswal M, Rajeswari N, Desai N, Jain S, Balakrishna N, Rao KV, Ahuja YR (1998) Risk assessment in cervical dysplasia patients by single cell gel electrophoresis assay: a study of DNA damage and repair. Mutat Res 412(2):195\u2013205","journal-title":"Mutat Res"},{"key":"5845_CR7","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1007\/978-1-4939-2013-6_22","volume":"1249","author":"EI Cort\u00e9s-Guti\u00e9rrez","year":"2015","unstructured":"Cort\u00e9s-Guti\u00e9rrez EI, Fern\u00e1ndez JL, D\u00e1vila-Rodr\u00edguez MI, L\u00f3pez-Fern\u00e1ndez C, Gos\u00e1lvez J (2015) Use of DBD-FISH for the study of cervical cancer progression. Methods Mol Biol 1249:291\u2013301","journal-title":"Methods Mol Biol"},{"issue":"4","key":"5845_CR8","first-page":"501","volume":"30","author":"SA Latt","year":"1978","unstructured":"Latt SA, Schreck RR, Loveday KS, Shuler CF (1978) In vitro and in vivo analysis of sister chromatid exchange. Pharmacol Rev 30(4):501\u2013535","journal-title":"Pharmacol Rev"},{"issue":"2","key":"5845_CR9","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/0165-1110(80)90014-7","volume":"75","author":"D Jenssen","year":"1980","unstructured":"Jenssen D, Ramel C (1980) The micronucleus test as part of a short term mutagenicity test program for the prediction of carcinogenicity evaluated by 143 agents tested. Mutat Res 75(2):191\u2013202","journal-title":"Mutat Res"},{"issue":"2","key":"5845_CR10","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/S1383-5718(02)00086-4","volume":"518","author":"CK Grisolia","year":"2002","unstructured":"Grisolia CK (2002) A comparison between mouse and fish micronucleus test using cyclophosphamide, mitomycin C and various pesticides. Mutat Res 518(2):145\u2013150","journal-title":"Mutat Res"},{"key":"5845_CR11","first-page":"203","volume":"203","author":"JL Fernandez","year":"2002","unstructured":"Fernandez JL, Gos\u00e1lvez J (2002) Application of FISH to detect DNA damage. DNA breakage detection-FISH (DBD-FISH). Methods Mol Biol 203:203\u2013216","journal-title":"Methods Mol Biol"},{"issue":"4","key":"5845_CR12","doi-asserted-by":"publisher","first-page":"e49","DOI":"10.4081\/ejh.2012.e49","volume":"56","author":"EI Cort\u00e9s-Gutierrez","year":"2012","unstructured":"Cort\u00e9s-Gutierrez EI, Davila-Rodriguez MI, Fernandez JL, Lopez-Fernandez C, Gos\u00e1lvez J (2012) DNA breakage detection fluorescence in situ hybridization in buccal cells. Eur J Histochem 56(4):e49","journal-title":"Eur J Histochem"},{"issue":"2","key":"5845_CR13","doi-asserted-by":"publisher","first-page":"4135","DOI":"10.3390\/ijms14024135","volume":"14","author":"EI Cort\u00e9s-Gutierrez","year":"2013","unstructured":"Cort\u00e9s-Gutierrez EI, Ortiz-Hernandez BL, Davila-Rodriguez MI, Cerda- Flores RM, Fernandez JL, Lopez-Fernandez C, Gosalvez J (2013) 5-bp classical satellite DNA loci from chromosome-1 instability in cervical neoplasia detected by DNA breakage detection\/fluorescence in situ hybridization (DBD-FISH). Int J Mol Sci 14(2):4135\u20134147","journal-title":"Int J Mol Sci"},{"key":"5845_CR14","volume-title":"Sperm chromatin","author":"J Gos\u00e1lvez","year":"2011","unstructured":"Gos\u00e1lvez J, L\u00f3pez-Fern\u00e1ndez C, Fern\u00e1ndez JL (2011) Sperm Chromatin dispersion test: technical aspects and clinical applications. In: Zini A, Agarwal A (eds) Sperm chromatin. Springer, New York"},{"issue":"5","key":"5845_CR15","first-page":"277","volume":"61","author":"P Barcena","year":"2015","unstructured":"Barcena P, L\u00f3pez-Fern\u00e1ndez C, Garc\u00eda-Ochoa C, Obradors A, Vernaev V, Gos\u00e1lvez J, Vassena R (2015) Detection of DNA damage in cumulus cells using a chromatin dispersion assay. Syst Biol Reprod Med 61(5):277\u2013285","journal-title":"Syst Biol Reprod Med"},{"issue":"spec num","key":"5845_CR16","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/ng0497supp-417","volume":"15","author":"F Mitelman","year":"1997","unstructured":"Mitelman F, Mertens F, Johansson B (1997) A breakpoint map of recurrent chromosomal rearrangements in human neoplasia. Nat Genet 15(spec num):417\u2013474","journal-title":"Nat Genet"},{"key":"5845_CR17","doi-asserted-by":"publisher","first-page":"385245","DOI":"10.1155\/2012\/385245","volume":"2012","author":"EI Cort\u00e9s-Guti\u00e9rrez","year":"2012","unstructured":"Cort\u00e9s-Guti\u00e9rrez EI, Hern\u00e1ndez-Garza F, Garc\u00eda-P\u00e9rez JO, D\u00e1vila-Rodr\u00edguez MI, Aguado-Barrera ME, Cerda-Flores RM (2012) Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques. J Biomed Biotechnol 2012:385245","journal-title":"J Biomed Biotechnol"},{"issue":"3","key":"5845_CR18","first-page":"54","volume":"48","author":"EI Cort\u00e9s-Guti\u00e9rrez","year":"2014","unstructured":"Cort\u00e9s-Guti\u00e9rrez EI, D\u00e1vila-Rodr\u00edguez MI, Cerda-Flores RM (2014) Chromosomal damage as prognosis marker in cervical carcinogenesis. Tsitol Genet 48(3):54\u201363","journal-title":"Tsitol Genet"},{"issue":"5035","key":"5845_CR19","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1126\/science.1659743","volume":"254","author":"H Zur Hausen","year":"1991","unstructured":"Zur Hausen H (1991) Viruses in human cancers. Science 254(5035):1167\u20131173","journal-title":"Science"},{"issue":"1","key":"5845_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/CMR.16.1.1-17.2003","volume":"16","author":"EM Burd","year":"2003","unstructured":"Burd EM (2003) Human papillomavirus and cervical cancer. Clin Microbiol Rev 16(1):1\u201317","journal-title":"Clin Microbiol Rev"},{"issue":"6712","key":"5845_CR21","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1038\/25292","volume":"396","author":"C Lengauer","year":"1998","unstructured":"Lengauer C, Kinzler KW, Vogelstein B (1998) Genetic instabilities in human cancers. Nature 396(6712):643\u2013649","journal-title":"Nature"},{"issue":"1","key":"5845_CR22","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1073\/pnas.93.1.479","volume":"93","author":"K Heselmeyer","year":"1996","unstructured":"Heselmeyer K, Schrock E, du Manoir S, Blegen H, Shah K, Steinbeck R, Auer G, Ried T (1996) Gain of chromosome 3q defines the transition from severe dysplasia to invasive carcinoma of the uterine cervix. Proc Natl Acad Sci USA 93(1):479\u2013484","journal-title":"Proc Natl Acad Sci USA"},{"issue":"2","key":"5845_CR23","first-page":"2773","volume":"61","author":"EI Cort\u00e9s-Gutierrez","year":"2017","unstructured":"Cort\u00e9s-Gutierrez EI, Garcia-Vielma C, Aguilar-Lemorroy A, Vallejo-Ruiz V, Pi\u00f1a-S\u00e1nchez P, Zapata-Benavides P, Gos\u00e1lvez J (2017) Expression of the HPV18\/E6 oncoprotein induces DNA damage. Eur J Histochem 61(2):2773","journal-title":"Eur J Histochem"},{"issue":"6","key":"5845_CR24","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1111\/j.1532-5415.1987.tb01400.x","volume":"35","author":"J Vijg","year":"1987","unstructured":"Vijg J, Knook DL (1987) DNA repair in relation to the aging process. J Am Geriatr Soc 35(6):532\u2013541","journal-title":"J Am Geriatr Soc"},{"issue":"1","key":"5845_CR25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0921-8734(89)90035-0","volume":"219","author":"TBL Kirkwood","year":"1989","unstructured":"Kirkwood TBL (1989) DNA mutation and aging. Mutat Res 219(1):1\u20137","journal-title":"Mutat Res"},{"issue":"4","key":"5845_CR26","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.cell.2005.01.028","volume":"120","author":"DB Lombard","year":"2005","unstructured":"Lombard DB, Chua KF, Mostoslavsky R, Franco S, Gostissa M, Alt FW (2005) DNA repair, genome stability, and aging. Cell 120(4):497\u2013512","journal-title":"Cell"},{"issue":"6","key":"5845_CR27","doi-asserted-by":"publisher","first-page":"432","DOI":"10.18632\/aging.100667","volume":"6","author":"JP Soares","year":"2014","unstructured":"Soares JP, Cortinha A, Bento T, Leitao JC, Collins AR, Gaivao I, Mota MP (2014) Aging and DNA damage in humans: a meta-analysis study. Aging 6(6):432\u2013439","journal-title":"Aging"},{"key":"5845_CR28","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.exger.2015.01.001","volume":"62","author":"JP Soares","year":"2015","unstructured":"Soares JP, Silva AM, Fonseca S, Oliveira MM, Peixoto F, Gaiv\u00e3o I, Mota MP (2015) How can age and lifestyle variables affect DNA damage, repair capacity and endogenous biomarkers of oxidative stress? Exp Gerontol 62:45\u201352","journal-title":"Exp Gerontol"},{"issue":"1","key":"5845_CR29","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1006\/gyno.1996.0285","volume":"63","author":"EE Sheets","year":"1996","unstructured":"Sheets EE, Crum CP, Yeh J (1996) Association between cervical neoplasia and apoptosis as detected by in situ nuclear labeling. Gynecol Oncol 63(1):94\u2013100","journal-title":"Gynecol Oncol"},{"issue":"9","key":"5845_CR30","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1038\/modpathol.3800927","volume":"20","author":"W Feng","year":"2007","unstructured":"Feng W, Xiao J, Zhang Z, Rosen DG, Brown RE, Liu J, Duan X (2007) Senescence and apoptosis in carcinogenesis of cervical squamous carcinoma. Mod Pathol 20(9):961\u2013966","journal-title":"Mod Pathol"},{"issue":"1","key":"5845_CR31","first-page":"214","volume":"1","author":"L Pray","year":"2008","unstructured":"Pray L (2008) DNA replication and causes of mutation. Nat Educ 1(1):214","journal-title":"Nat Educ"},{"key":"5845_CR32","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1016\/j.freeradbiomed.2016.11.039","volume":"107","author":"R Abbotts","year":"2017","unstructured":"Abbotts R, Wilson DM 3rd (2017) Coordination of DNA single strand break repair. Free Radical Biol Med 107:228\u2013244","journal-title":"Free Radical Biol Med"},{"issue":"6","key":"5845_CR33","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1023\/A:1020811522100","volume":"18","author":"V Calini","year":"2002","unstructured":"Calini V, Urani C, Camatini M (2002) Comet assay evaluation of DNA single- and double-strand breaks induction and repair in C3H10T1\/2 cells. Cell Biol Toxicol 18(6):369\u2013379","journal-title":"Cell Biol Toxicol"},{"issue":"10","key":"5845_CR34","doi-asserted-by":"publisher","first-page":"5357","DOI":"10.1073\/pnas.090013497","volume":"97","author":"D Gisselsson","year":"2002","unstructured":"Gisselsson D, Pettersson L, Hoglund M, Heidenblad M, Gorunova L, Wiegant J, Mertens F, Dal Cin P, Mitlman F, Mandahl N (2002) Chromosomal breakage fusionbridge events cause genetic intratumor heterogeneity. Proc Natl Acad Sci USA 97(10):5357\u20135362","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5","key":"5845_CR35","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1002\/ijc.10627","volume":"101","author":"A Zhang","year":"2002","unstructured":"Zhang A, M\u00e1ner S, Betz R, Angstrom T, Stendahl U, Bergman F, Zetterberg A, Wallin KL (2002) Genetic alterations in cervical carcinomas: Frequent low-level amplifications of oncogenes are associated with human papillomavirus infection. Int J Cancer 101(5):427\u2013433","journal-title":"Int J Cancer"},{"issue":"1","key":"5845_CR36","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1089\/scd.2016.0209","volume":"26","author":"L Barrachina","year":"2017","unstructured":"Barrachina L, Remacha AR, Romero A, Vazquez FJ, Albareda J, Prades M, Gos\u00e1lvez J, Roy R, Zaragoza P, Mart\u00edn-Burriel I, Rodellar C (2017) Priming equine bone marrow-derived mesenchymal stem cells with proinflammatory cytokines: implications in immunomodulation-immunogenicity balance, cell viability, and differentiation potential. Stem Cells Dev 26(1):15\u201324","journal-title":"Stem Cells Dev"},{"key":"5845_CR37","volume-title":"A clinician\u00b4s guide to sperm DNA and chromatin damage","author":"JL Fern\u00e1ndez","year":"2018","unstructured":"Fern\u00e1ndez JL, Johnston S, Gos\u00e1lvez J (2018) Sperm chromatin dispersion (SCD) assay. In: Zini A, Agarwal A (eds) A clinician\u00b4s guide to sperm DNA and chromatin damage. Springer, Cham"},{"key":"5845_CR38","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1007\/978-1-4939-0820-2_15","volume":"753","author":"J Gos\u00e1lvez","year":"2014","unstructured":"Gos\u00e1lvez J, Holt WV, Johnston SD (2014) Sperm DNA fragmentation and its role in wildlife conservation. Adv Exp Med Biol 753:357\u2013384","journal-title":"Adv Exp Med Biol"},{"issue":"2","key":"5845_CR39","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1089\/mdr.2016.0023","volume":"23","author":"F Otero","year":"2017","unstructured":"Otero F, Santiso R, Tamayo M, Fern\u00e1ndez JL, Bou G, Lepe JA, McConnell M, Gos\u00e1lvez J, Cisneros JM (2017) Rapid detection of antibiotic resistance in Gram negative bacteria through assessment of changes in cellular morphology. Microb Drug Resist 23(2):157\u2013162","journal-title":"Microb Drug Resist"},{"issue":"11","key":"5845_CR40","doi-asserted-by":"publisher","first-page":"3033","DOI":"10.1158\/1055-9965.EPI-08-0508","volume":"17","author":"A Szarewski","year":"2008","unstructured":"Szarewski A, Ambroisine L, Cadman L, Austin J, Ho I, Terry G, Liddle S, Dina R, McCarthy J, Buckley H, Bergeron C, Soutter P, Deirdre L, Cuzick J (2008) Comparison of predictors for high-grade cervical intraepithelial neoplasia in women with abnormal smears. Cancer Epidemiol Biomarkers Prev 17(11):3033\u20133042","journal-title":"Cancer Epidemiol Biomarkers Prev"}],"container-title":["Archives of Gynecology and Obstetrics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00404-020-05845-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00404-020-05845-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00404-020-05845-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T13:05:46Z","timestamp":1616418346000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00404-020-05845-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,23]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["5845"],"URL":"https:\/\/doi.org\/10.1007\/s00404-020-05845-3","relation":{},"ISSN":["0932-0067","1432-0711"],"issn-type":[{"value":"0932-0067","type":"print"},{"value":"1432-0711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,23]]},"assertion":[{"value":"3 March 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declared that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"From all human participants Informed consent was obtained.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}