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This cohort study examines the persistence of high-risk cfDNA in the third pregnancy trimester after exclusion of fetal involvement, and the concordance of these results with CPM in the postpartum placenta.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Pregnant individuals receiving a false-positive primary cfDNA result were recruited from Monash Health and Monash Ultrasound for Women in Melbourne, Australia, between August 2023 and December 2024. Participants underwent genome-wide repeat cfDNA screening (\n                      <jats:italic>r<\/jats:italic>\n                      -cfDNA) after 30\u2009+\u20090\u00a0weeks\u2019 gestation. Placental samples were collected and cytogenetically analysed postpartum.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      This cohort included 21 individuals, of which 33.3% (7\/21) screened high-risk on\n                      <jats:italic>r<\/jats:italic>\n                      -cfDNA. There was no significant association between\n                      <jats:italic>r<\/jats:italic>\n                      -cfDNA and CPM in the postpartum placenta (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.397), as five (5\/12, 41.7%) cases involving CPM were screened \u2018low-risk\u2019. Fetal-fraction was significantly lower (5.0% [IQR\u2009=\u20094.6\u20138.0%] vs. 9.0% [IQR\u2009=\u20097.0\u201313.5%],\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.025), and maternal BMI (Kg\/m2) higher (29.0 [IQR\u2009=\u200925.7\u201331.2] vs. 23.3 [IQR\u2009=\u200922.0\u201324.9],\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.006), in false-positive cases not attributable to CPM detected by\n                      <jats:italic>r<\/jats:italic>\n                      -cfDNA or postpartum. High-risk\n                      <jats:italic>r<\/jats:italic>\n                      -cfDNA results were associated with smaller babies (median birthweight percentile\u2009=\u200912.3 [IQR\u2009=\u20094.4\u201321.7] vs. 31.9 [IQR\u2009=\u200921.5\u201355.5],\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.009), though the majority of outcomes were favorable.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      High-risk\n                      <jats:italic>r<\/jats:italic>\n                      -cfDNA is not a sensitive predictor of CPM, though high mosaic ratios are associated with adverse obstetric outcomes. An alternative explanation for false-positive cfDNA besides CPM may be low fetal fraction, particularly in the context of high maternal BMI.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00404-025-08162-9","type":"journal-article","created":{"date-parts":[[2025,8,31]],"date-time":"2025-08-31T10:59:33Z","timestamp":1756637973000},"page":"1679-1687","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Repeat cell-free DNA screening after initial false-positive results and term placental analysis for confined mosaicism: a prospective cohort study"],"prefix":"10.1007","volume":"312","author":[{"given":"Yvette C.","family":"Raymond","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shavi","family":"Fernando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melody","family":"Menezes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben W.","family":"Mol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tristan","family":"Hardy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kara","family":"Levin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"McLennan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Lorber Rolnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,8,31]]},"reference":[{"issue":"4","key":"8162_CR1","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1086\/301800","volume":"62","author":"YD Lo","year":"1998","unstructured":"Lo YD, Tein MS, Lau TK, Haines CJ, Leung TN, Poon PM et al (1998) Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis. 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