{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:35:05Z","timestamp":1777620905311,"version":"3.51.4"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870710"],"award-info":[{"award-number":["81870710"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur Arch Otorhinolaryngol"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1007\/s00405-021-06997-x","type":"journal-article","created":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T09:03:12Z","timestamp":1626944592000},"page":"4893-4899","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The integrity and barrier function of porcine vocal fold epithelium: its susceptibility to damage by deoxycholic acid compared with pepsin"],"prefix":"10.1007","volume":"278","author":[{"given":"Zhewei","family":"Lou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Silverman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaxia","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3070-4952","authenticated-orcid":false,"given":"Jack J.","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,7,22]]},"reference":[{"issue":"4","key":"6997_CR1","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1007\/s00405-011-1905-3","volume":"269","author":"M Saruc","year":"2012","unstructured":"Saruc M, Aksoy EA, Vardereli E, Karaaslan M, Cicek B, Ince U, Oz F, Tozun N (2012) Risk factors for laryngopharyngeal reflux. Eur Arch Otorhinolaryngol 269(4):1189\u20131194","journal-title":"Eur Arch Otorhinolaryngol"},{"issue":"1","key":"6997_CR2","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/S0194-5998(97)70350-4","volume":"116","author":"DC Metz","year":"1997","unstructured":"Metz DC, Childs ML, Ruiz C, Weinstein GS (1997) Pilot study of the oral omeprazole test for reflux laryngitis. Otolaryngol Head Neck Surg 116(1):41\u201346","journal-title":"Otolaryngol Head Neck Surg"},{"issue":"11","key":"6997_CR3","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1097\/00007611-199711000-00012","volume":"90","author":"GY Shaw","year":"1997","unstructured":"Shaw GY, Searl JP (1997) Laryngeal manifestations of gastroesophageal reflux before and after treatment with omeprazole. South Med J 90(11):1115\u20131122","journal-title":"South Med J"},{"issue":"12","key":"6997_CR4","first-page":"2160","volume":"92","author":"JM Wo","year":"1997","unstructured":"Wo JM, Grist WJ, Gussack G, Delgaudio JM, Waring JP (1997) Empiric trial of high-dose omeprazole in patients with posterior laryngitis: a prospective study. Am J Gastroenterol 92(12):2160\u20132165","journal-title":"Am J Gastroenterol"},{"issue":"5","key":"6997_CR5","doi-asserted-by":"publisher","first-page":"A636","DOI":"10.1016\/S0016-5085(01)80581-3","volume":"120","author":"MF Vaezi","year":"2001","unstructured":"Vaezi MF, Hicks DM, Ours TM, Richter JE (2001) ENT manifestations of GERD: a large prospective study assessing treatment outcome and predictors of response. Gastroenterology 120(5):A636","journal-title":"Gastroenterology"},{"issue":"12","key":"6997_CR6","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1136\/jcp.2004.016972","volume":"58","author":"GA Gill","year":"2005","unstructured":"Gill GA, Buda A, Moorghen M, Dettmar PW, Pignatelli M (2005) Characterisation of adherens and tight junctional molecules in normal animal larynx; determining a suitable model for studying molecular abnormalities in human laryngopharyngeal reflux. J Clin Pathol 58(12):1265\u20131270","journal-title":"J Clin Pathol"},{"issue":"4","key":"6997_CR7","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1002\/lary.20665","volume":"120","author":"DM Bulmer","year":"2010","unstructured":"Bulmer DM, Ali MS, Brownlee IA, Dettmar PW, Pearson JP (2010) Laryngeal mucosa: its susceptibility to damage by acid and pepsin. Laryngoscope 120(4):777\u2013782","journal-title":"Laryngoscope"},{"issue":"5","key":"6997_CR8","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1007\/s00232-014-9651-2","volume":"247","author":"EE Levendoski","year":"2014","unstructured":"Levendoski EE, Sivasankar MP (2014) Vocal fold ion transport and mucin expression following acrolein exposure. J Membr Biol 247(5):441\u2013450","journal-title":"J Membr Biol"},{"issue":"11","key":"6997_CR9","doi-asserted-by":"publisher","first-page":"2098","DOI":"10.1111\/j.1572-0241.2004.40170.x","volume":"99","author":"T Adhami","year":"2004","unstructured":"Adhami T, Goldblum JR, Richter JE, Vaezi MF (2004) The role of gastric and duodenal agents in laryngeal injury: an experimental canine model. Am J Gastroenterol 99(11):2098\u20132106","journal-title":"Am J Gastroenterol"},{"issue":"1","key":"6997_CR10","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/S0194-5998(99)70155-5","volume":"121","author":"MY Chen","year":"1999","unstructured":"Chen MY, Ott DJ, Koufman JA (1999) Correlation of laryngeal and pharyngeal carcinomas and 24\u00a0hour pH monitoring of the esophagus and pharynx. Otolaryngol Head Neck Surg 121(1):168","journal-title":"Otolaryngol Head Neck Surg"},{"issue":"12","key":"6997_CR11","first-page":"1503","volume":"84","author":"GJ Wiener","year":"1989","unstructured":"Wiener GJ, Koufman JA, Wu WC, Cooper JB, Richter JE, Castell DO (1989) Chronic hoarseness secondary to gastroesophageal reflux disease: documentation with 24\u00a0h ambulatory pH monitoring. Am J Gastroenterol 84(12):1503\u20131508","journal-title":"Am J Gastroenterol"},{"issue":"5 Pt 1","key":"6997_CR12","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1177\/000348948509400521","volume":"94","author":"FB Little","year":"1985","unstructured":"Little FB, Koufman JA, Kohut RI, Marshall RB (1985) Effect of gastric acid on the pathogenesis of subglottic stenosis. Ann Otol Rhinol Laryngol 94(5 Pt 1):516\u2013519","journal-title":"Ann Otol Rhinol Laryngol"},{"issue":"6","key":"6997_CR13","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1007\/s00405-018-4973-9","volume":"275","author":"C Zhang","year":"2018","unstructured":"Zhang C, Paddock K, Chou A, Scholp A, Gong T, Jiang JJ (2018) Prolonged phonation impairs the integrity and barrier function of porcine vocal fold epithelium: a preliminary study. Eur Arch Otorhinolaryngol 275(6):1547\u20131556","journal-title":"Eur Arch Otorhinolaryngol"},{"issue":"6","key":"6997_CR14","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1177\/000348940311200601","volume":"112","author":"N Johnston","year":"2003","unstructured":"Johnston N, Bulmer D, Gill GA, Panetti M, Ross PE, Pearson JP, Pignatelli M, Axford SE, Dettmar PW, Koufman JA (2003) Cell biology of laryngeal epithelial defenses in health and disease: further studies. Ann Otol Rhinol Laryngol 112(6):481\u2013491","journal-title":"Ann Otol Rhinol Laryngol"},{"issue":"5","key":"6997_CR15","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1136\/gut.44.5.598","volume":"44","author":"D Nehra","year":"1999","unstructured":"Nehra D, Howell P, Williams CP, Pye JK, Beynon J (1999) Toxic bile acids in gastro-oesophageal reflux disease: influence of gastric acidity. Gut 44(5):598\u2013602","journal-title":"Gut"},{"issue":"1","key":"6997_CR16","first-page":"79","volume":"84","author":"JW Harmon","year":"1978","unstructured":"Harmon JW, Doong T, Gadacz TR (1978) Bile acids are not equally damaging to the gastric mucosa. Surgery 84(1):79","journal-title":"Surgery"},{"issue":"10","key":"6997_CR17","doi-asserted-by":"publisher","first-page":"1366","DOI":"10.1136\/gut.2007.141804","volume":"57","author":"R Farre","year":"2008","unstructured":"Farre R, van Malenstein H, De Vos R, Geboes K, Depoortere I, Vanden Berghe P, Fornari F, Blondeau K, Mertens V, Tack J, Sifrim D (2008) Short exposure of oesophageal mucosa to bile acids, both in acidic and weakly acidic conditions, can impair mucosal integrity and provoke dilated intercellular spaces. Gut 57(10):1366\u20131374","journal-title":"Gut"},{"issue":"8","key":"6997_CR18","doi-asserted-by":"publisher","first-page":"1900","DOI":"10.1111\/j.1572-0241.2006.00630.x","volume":"101","author":"N Vakil","year":"2006","unstructured":"Vakil N, van Zanten SV, Kahrilas P, Dent J, Jones R, Global Consensus G (2006) The Montreal definition and classification of gastroesophageal reflux disease: a global evidence-based consensus. Am J Gastroenterol 101(8):1900\u20131920 (quiz 1943)","journal-title":"Am J Gastroenterol"},{"issue":"1","key":"6997_CR19","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1002\/lary.28643","volume":"131","author":"CA McCormick","year":"2021","unstructured":"McCormick CA, Samuels TL, Battle MA, Frolkis T, Blumin JH, Bock JM, Wells C, Yan K, Altman KW, Johnston N (2021) H+\/K+ATPase expression in the larynx of laryngopharyngeal reflux and laryngeal cancer patients. Laryngoscope 131(1):130\u2013135","journal-title":"Laryngoscope"},{"issue":"11","key":"6997_CR20","doi-asserted-by":"publisher","first-page":"1941","DOI":"10.1288\/00005537-196811000-00008","volume":"78","author":"JE Delahunty","year":"1968","unstructured":"Delahunty JE, Cherry J (1968) Experimentally produced vocal cord granulomas. Laryngoscope 78(11):1941\u20131947","journal-title":"Laryngoscope"},{"issue":"4 Pt 2 Suppl 53","key":"6997_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/lary.1991.101.s53.1","volume":"101","author":"JA Koufman","year":"1991","unstructured":"Koufman JA (1991) The otolaryngologic manifestations of gastroesophageal reflux disease (GERD): a clinical investigation of 225 patients using ambulatory 24\u00a0hour pH monitoring and an experimental investigation of the role of acid and pepsin in the development of laryngeal injury. Laryngoscope 101(4 Pt 2 Suppl 53):1\u201378","journal-title":"Laryngoscope"},{"issue":"7","key":"6997_CR22","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1080\/00365521.1984.12005823","volume":"19","author":"JA Salo","year":"1984","unstructured":"Salo JA, Kivilaakso E (1984) Contribution of trypsin and cholate to the pathogenesis of experimental alkaline reflux esophagitis. Scand J Gastroenterol 19(7):875\u2013881","journal-title":"Scand J Gastroenterol"},{"issue":"3","key":"6997_CR23","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/S1091-255X(98)80021-8","volume":"2","author":"M Fein","year":"1998","unstructured":"Fein M, Peters JH, Chandrasoma P, Ireland AP, Oberg S, Ritter MP, Bremner CG, Hagen JA, DeMeester TR (1998) Duodenoesophageal reflux induces esophageal adenocarcinoma without exogenous carcinogen. J Gastrointest Surg 2(3):260\u2013268","journal-title":"J Gastrointest Surg"},{"issue":"1","key":"6997_CR24","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/S1091-255X(00)80032-3","volume":"4","author":"J Theisen","year":"2000","unstructured":"Theisen J, Nehra D, Citron D, Johansson J, Hagen JA, Crookes PF, DeMeester SR, Bremner CG, DeMeester TR, Peters JH (2000) Suppression of gastric acid secretion in patients with gastroesophageal reflux disease results in gastric bacterial overgrowth and deconjugation of bile acids. J Gastrointest Surg 4(1):50\u201354","journal-title":"J Gastrointest Surg"},{"issue":"5","key":"6997_CR25","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1093\/glycob\/cwr190","volume":"22","author":"AM Byrne","year":"2012","unstructured":"Byrne AM, Sharma R, Duggan G, Kelleher D, Long A (2012) Deoxycholic acid impairs glycosylation and fucosylation processes in esophageal epithelial cells. Glycobiology 22(5):638\u2013648","journal-title":"Glycobiology"},{"issue":"2","key":"6997_CR26","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1208\/s12249-011-9621-2","volume":"12","author":"T Caon","year":"2011","unstructured":"Caon T, Simoes CM (2011) Effect of freezing and type of mucosa on ex vivo drug permeability parameters. AAPS PharmSciTech 12(2):587\u2013592","journal-title":"AAPS PharmSciTech"},{"issue":"12","key":"6997_CR27","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.3109\/02713683.2014.904361","volume":"39","author":"XC Gao","year":"2014","unstructured":"Gao XC, Qi HP, Bai JH, Huang L, Cui H (2014) Effects of oleic acid on the corneal permeability of compounds and evaluation of its ocular irritation of rabbit eyes. Curr Eye Res 39(12):1161\u20131168","journal-title":"Curr Eye Res"},{"issue":"7","key":"6997_CR28","doi-asserted-by":"publisher","first-page":"G487","DOI":"10.1152\/ajpgi.00370.2015","volume":"310","author":"NA Pardon","year":"2016","unstructured":"Pardon NA, Vicario M, Vanheel H, Vanuytsel T, Ceulemans LJ, Vieth M, Jimenez M, Tack J, Farre R (2016) A weakly acidic solution containing deoxycholic acid induces esophageal epithelial apoptosis and impairs integrity in an in vivo perfusion rabbit model. Am J Physiol Gastrointest Liver Physiol 310(7):G487-496","journal-title":"Am J Physiol Gastrointest Liver Physiol"}],"container-title":["European Archives of Oto-Rhino-Laryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00405-021-06997-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00405-021-06997-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00405-021-06997-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T03:38:34Z","timestamp":1635478714000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00405-021-06997-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,22]]},"references-count":28,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["6997"],"URL":"https:\/\/doi.org\/10.1007\/s00405-021-06997-x","relation":{},"ISSN":["0937-4477","1434-4726"],"issn-type":[{"value":"0937-4477","type":"print"},{"value":"1434-4726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,22]]},"assertion":[{"value":"21 May 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 July 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"No conflict of interest exists in the submission of this manuscript.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Ethical approval was obtained from the ethics committee of Eye, Ear, Nose and Throat Hospital of Fudan University (2017061).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Informed consent was obtained from all individual participants included in the study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}