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Next, we analyzed the relationship between Glut-1 and H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase expression with the clinicopathological features of laryngeal carcinoma.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Glut-1 and H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase expression levels in HVCLCs were determined after treatment with artificial gastric juice containing pepsin and laryngeal carcinoma tissues.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Exposure to pepsin-containing artificial gastric juice significantly enhanced the migration and proliferation of VSCLCs in a time-dependent manner. The apoptotic rate of VSCLCs decreased over time after exposure to pepsin and reached a nadir on day 7 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). With increasing duration of exposure to pepsin, the proportion of VSCLCs in G0\/G1 phase decreased and the proportions in the S and G2\/M phases significantly increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). After treatment with pepsin-containing artificial gastric juice, RT-PCR and Western blotting showed that the expression of Glut-1 and H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase \u03b1, \u03b2 significantly increased in HVCLCs compared to in the absence of pepsin (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). The expression of Glut-1 and H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase \u03b1, \u03b2 gradually increased from vocal cord leukoplakia (VLC) to laryngeal carcinoma (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Lentivirus-mediated inhibition of Glut-1 expression in VCL significantly inhibited the cells\u2019 migration and proliferation (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) but enhanced their apoptosis (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Also, inhibition of Glut-1 expression resulted in an increased proportion of cells in G0\/G1 phase and a significantly decreased proportion in G2\/M phase (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Elevated Glut-1 expression may promote the development of VCL by upregulating laryngeal H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase expression to reactivate absorbed pepsin, thus damaging the laryngeal mucosa.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00405-021-07172-y","type":"journal-article","created":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T15:02:26Z","timestamp":1637420546000},"page":"1413-1424","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Role and mechanism of Glut-1 and H+\/K+-ATPase expression in pepsin-induced development of vocal cord leukoplakia"],"prefix":"10.1007","volume":"279","author":[{"given":"Yin-Jie","family":"Ao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Ting","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zai-Zai","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7163-2289","authenticated-orcid":false,"given":"Shui-Hong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang-Yang","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Fang","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,11,20]]},"reference":[{"issue":"1","key":"7172_CR1","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1177\/0194599820938654","volume":"164","author":"YL Chen","year":"2021","unstructured":"Chen YL, Bao YY, Zhou SH, Yao HT, Chen Z (2021) Relationship between pepsin expression and dysplasia grade in patients with vocal cord leukoplakia. 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