{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T09:02:05Z","timestamp":1780995725057,"version":"3.54.1"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"The Swiss National Foundation","award":["320030_189280"],"award-info":[{"award-number":["320030_189280"]}]},{"name":"Astra Zeneca"},{"name":"Clinic of Pneumology, University Hospital Basel"},{"name":"Universit\u00e4tsklinikum Freiburg"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Lung"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Purpose<\/jats:title>\n                <jats:p>The response to glucocorticoids is hampered in many COPD patients by a yet unknown mechanism. Earlier we reported that short-term heat exposure of primary human bronchial epithelial cells (BEC) and airway smooth muscle cells (ASMC) of asthma patients increased the expression and secretion of extracellular heat shock proteins (eHSPs) resulting in increased expression of glucocorticoid receptor (GR) in BEC and inhibition of ASMC remodeling. The aim of the present study was to assess if the same mechanism is also present in primary airway wall cells of COPD patients.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Primary BEC and ASMC were established from endobronchial biopsies obtained from COPD patients (<jats:italic>n<\/jats:italic>\u2009=\u200973), who participated in the HISTORIC study, an investigator-initiated and driven clinical trial. Secretion and protein expression of HSPs was assessed by ELISA and Western blotting. Expression of total GR, its isoforms GR\u03b1 and GR\u03b2 and toll-like receptor 4 (TLR4) was determined by Western-blotting.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Short heat exposure (65\u00a0\u00b0C, 10\u00a0s) of BEC resulted in a significant increase of the secretion of eHSP70 and eHSP90, while the intracellular protein was not altered. Heat treatment or exposure to eHSP70 or eHSP90 had no effect on the expression of GR and GR-isoforms. However, eHSP70 and eHSP90 significantly reduced the expression of TLR4.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The results of this study indicate that primary airway cells from COPD patients respond differently to heat exposure and extracellular HSP70 or HSP90 than cells from asthma patients regarding the expression of GR and this may explain the reduced response to glucocorticoids in patients with COPD.<\/jats:p>\n                <jats:p><jats:italic>Trial Registration<\/jats:italic>: ISRCTN11017699<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00408-024-00680-8","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T20:33:51Z","timestamp":1713558831000},"page":"235-243","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Heat-Induced Secretion of Heat Shock Proteins 70 and 90 Does not Affect the Expression of the Glucocorticoid Receptor in Primary Airway Cells in COPD"],"prefix":"10.1007","volume":"202","clinical-trial-number":[{"clinical-trial-number":"isrctn11017699","registry":"10.18810\/isrctn"}],"author":[{"given":"Liang","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Roth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni","family":"Papakonstantinou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Tamm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiana","family":"Stolz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,19]]},"reference":[{"issue":"5","key":"680_CR1","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1016\/S2213-2600(21)00555-5","volume":"10","author":"A Agusti","year":"2022","unstructured":"Agusti A, Melen E, DeMeo DL, Breyer-Kohansal R, Faner R (2022) Pathogenesis of chronic obstructive pulmonary disease: understanding the contributions of gene-environment interactions across the lifespan. Lancet Respir Med 10(5):512\u2013524","journal-title":"Lancet Respir Med"},{"issue":"4","key":"680_CR2","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1183\/13993003.00239-2023","volume":"61","author":"A Agusti","year":"2023","unstructured":"Agusti A, Celli BR, Criner GJ, Halpin D, Anzueto A, Barnes P et al (2023) Global initiative for chronic obstructive lung disease 2023 report: GOLD executive summary. Eur Respir J 61(4):819\u2013837","journal-title":"Eur Respir J"},{"issue":"2 Pt 1","key":"680_CR3","first-page":"342","volume":"161","author":"PJ Barnes","year":"2000","unstructured":"Barnes PJ (2000) Inhaled corticosteroids are not beneficial in chronic obstructive pulmonary disease. Am J Respir Crit Care Med 161(2 Pt 1):342\u2013344","journal-title":"Am J Respir Crit Care Med"},{"issue":"13","key":"680_CR4","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1056\/NEJMra1900475","volume":"381","author":"A Agusti","year":"2019","unstructured":"Agusti A, Hogg JC (2019) Update on the pathogenesis of chronic obstructive pulmonary disease. N Engl J Med 381(13):1248\u20131256","journal-title":"N Engl J Med"},{"issue":"7893","key":"680_CR5","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1038\/s41586-021-04252-1","volume":"601","author":"RY Wang","year":"2022","unstructured":"Wang RY, Noddings CM, Kirschke E, Myasnikov AG, Johnson JL, Agard DA (2022) Structure of Hsp90-Hsp70-Hop-GR reveals the Hsp90 client-loading mechanism. Nature 601(7893):460\u2013464","journal-title":"Nature"},{"issue":"3","key":"680_CR6","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1016\/j.amjmed.2004.05.006","volume":"117","author":"GP Chrousos","year":"2004","unstructured":"Chrousos GP (2004) The glucocorticoid receptor gene, longevity, and the complex disorders of western societies. Am J Med 117(3):204\u2013207","journal-title":"Am J Med"},{"key":"680_CR7","first-page":"229","volume-title":"Encyclopedia of Endocrine diseases","author":"Chrousos ECTKGP","year":"2004","unstructured":"Chrousos ECTKGP (2004) Glucocorticoid receptor. In: Martini L (ed) Encyclopedia of Endocrine diseases. Elsevier, Amsterdam, pp 229\u2013234"},{"issue":"12","key":"680_CR8","doi-asserted-by":"publisher","first-page":"3062","DOI":"10.1158\/1535-7163.MCT-06-0344","volume":"5","author":"B Sanchez-Vega","year":"2006","unstructured":"Sanchez-Vega B, Krett N, Rosen ST, Gandhi V (2006) Glucocorticoid receptor transcriptional isoforms and resistance in multiple myeloma cells. Mol Cancer Ther 5(12):3062\u20133070","journal-title":"Mol Cancer Ther"},{"issue":"39","key":"680_CR9","doi-asserted-by":"publisher","first-page":"27857","DOI":"10.1074\/jbc.274.39.27857","volume":"274","author":"RH Oakley","year":"1999","unstructured":"Oakley RH, Jewell CM, Yudt MR, Bofetiado DM, Cidlowski JA (1999) The dominant negative activity of the human glucocorticoid receptor beta isoform. Specificity and mechanisms of action. J Biol Chem 274(39):27857\u201327866","journal-title":"J Biol Chem"},{"issue":"1","key":"680_CR10","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1515\/HMBCI.2010.039","volume":"3","author":"D Duma","year":"2010","unstructured":"Duma D, Cidlowski JA (2010) Generating diversity in glucocorticoid receptor signaling: mechanisms, receptor isoforms, and post-translational modifications. Horm Mol Biol Clin Investig 3(1):319\u2013328","journal-title":"Horm Mol Biol Clin Investig"},{"issue":"1","key":"680_CR11","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/S0952-7915(01)00297-7","volume":"14","author":"Z Li","year":"2002","unstructured":"Li Z, Menoret A, Srivastava P (2002) Roles of heat-shock proteins in antigen presentation and cross-presentation. Curr Opin Immunol 14(1):45\u201351","journal-title":"Curr Opin Immunol"},{"issue":"11","key":"680_CR12","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1038\/s41580-019-0133-3","volume":"20","author":"R Rosenzweig","year":"2019","unstructured":"Rosenzweig R, Nillegoda NB, Mayer MP, Bukau B (2019) The Hsp70 chaperone network. Nat Rev Mol Cell Biol 20(11):665\u2013680","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"6","key":"680_CR13","doi-asserted-by":"publisher","first-page":"2109","DOI":"10.1074\/jbc.REV118.002806","volume":"294","author":"O Genest","year":"2019","unstructured":"Genest O, Wickner S, Doyle SM (2019) Hsp90 and Hsp70 chaperones: collaborators in protein remodeling. J Biol Chem 294(6):2109\u20132120","journal-title":"J Biol Chem"},{"issue":"5","key":"680_CR14","doi-asserted-by":"publisher","first-page":"1249","DOI":"10.1016\/j.jaci.2021.02.022","volume":"148","author":"L Fang","year":"2021","unstructured":"Fang L, Li J, Papakonstantinou E, Karakioulaki M, Sun Q, Schumann D et al (2021) Secreted heat shock proteins control airway remodeling: Evidence from bronchial thermoplasty. J Allergy Clin Immunol 148(5):1249\u20131261","journal-title":"J Allergy Clin Immunol"},{"issue":"1","key":"680_CR15","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1186\/s12931-021-01774-0","volume":"22","author":"E Papakonstantinou","year":"2021","unstructured":"Papakonstantinou E, Koletsa T, Zhou L, Fang L, Roth M, Karakioulaki M et al (2021) Bronchial thermoplasty in asthma: an exploratory histopathological evaluation in distinct asthma endotypes\/phenotypes. Respir Res 22(1):186","journal-title":"Respir Res"},{"issue":"14","key":"680_CR16","doi-asserted-by":"publisher","first-page":"11700","DOI":"10.3390\/ijms241411700","volume":"24","author":"L Zhou","year":"2023","unstructured":"Zhou L, Fang L, Tamm M, Stolz D, Roth M (2023) Extracellular heat shock protein 70 increases the glucocorticoid receptor and dual-specificity phosphatase 1 via toll-like receptor 4 and attenuates inflammation in airway epithelial cells. Int J Mol Sci 24(14):11700","journal-title":"Int J Mol Sci"},{"issue":"1","key":"680_CR17","doi-asserted-by":"publisher","first-page":"2350218","DOI":"10.1183\/13993003.00218-2023","volume":"62","author":"D Stolz","year":"2023","unstructured":"Stolz D, Papakonstantinou E, Pascarella M, Jahn K, Siebeneichler A, Darie AM et al (2023) Airway smooth muscle area to predict steroid responsiveness in COPD patients receiving triple therapy (HISTORIC): a randomised, placebo-controlled, double-blind, investigator-initiated trial. Eur Respir J. 62(1):2350218","journal-title":"Eur Respir J."},{"issue":"7","key":"680_CR18","doi-asserted-by":"publisher","first-page":"1737","DOI":"10.1111\/all.15714","volume":"78","author":"A Yorgancioglu","year":"2023","unstructured":"Yorgancioglu A, Reddel HK, Directors GBo, Committee GS (2023) Global initiative for asthma: 30 years of promoting evidence-based asthma care. Allergy. 78(7):1737\u20131739","journal-title":"Allergy."},{"key":"680_CR19","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1146\/annurev.pathol.4.110807.092145","volume":"4","author":"JC Hogg","year":"2009","unstructured":"Hogg JC, Timens W (2009) The pathology of chronic obstructive pulmonary disease. Annu Rev Pathol 4:435\u2013459","journal-title":"Annu Rev Pathol"},{"issue":"3","key":"680_CR20","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1038\/nri2254","volume":"8","author":"PJ Barnes","year":"2008","unstructured":"Barnes PJ (2008) Immunology of asthma and chronic obstructive pulmonary disease. Nat Rev Immunol 8(3):183\u2013192","journal-title":"Nat Rev Immunol"},{"issue":"7","key":"680_CR21","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1152\/japplphysiol.00950.2012","volume":"114","author":"DC Doeing","year":"2013","unstructured":"Doeing DC, Solway J (2013) Airway smooth muscle in the pathophysiology and treatment of asthma. J Appl Physiol 114(7):834\u2013843","journal-title":"J Appl Physiol"},{"issue":"8","key":"680_CR22","doi-asserted-by":"publisher","first-page":"1551","DOI":"10.1038\/sj.leu.2403027","volume":"17","author":"M Lauten","year":"2003","unstructured":"Lauten M, Beger C, Gerdes K, Asgedom G, Kardinal C, Welte K et al (2003) Expression of heat-shock protein 90 in glucocorticoid-sensitive and -resistant childhood acute lymphoblastic leukaemia. Leukemia 17(8):1551\u20131556","journal-title":"Leukemia"},{"issue":"1","key":"680_CR23","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.yexcr.2012.09.018","volume":"319","author":"KH Lee","year":"2013","unstructured":"Lee KH, Jeong J, Yoo CG (2013) Positive feedback regulation of heat shock protein 70 (Hsp70) is mediated through Toll-like receptor 4-PI3K\/Akt-glycogen synthase kinase-3beta pathway. Exp Cell Res 319(1):88\u201395","journal-title":"Exp Cell Res"},{"issue":"2","key":"680_CR24","doi-asserted-by":"publisher","first-page":"229","DOI":"10.3390\/cells11020229","volume":"11","author":"CS Fan","year":"2022","unstructured":"Fan CS, Chen CC, Chen LL, Chua KV, Hung HC, Hsu JT et al (2022) Extracellular HSP90alpha induces MyD88-IRAK complex-associated IKKalpha\/beta-NF-kappaB\/IRF3 and JAK2\/TYK2-STAT-3 signaling in macrophages for tumor-promoting M2-polarization. Cells 11(2):229","journal-title":"Cells"},{"issue":"2","key":"680_CR25","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1002\/1521-4141(200202)32:2<322::AID-IMMU322>3.0.CO;2-0","volume":"32","author":"D Lipsker","year":"2002","unstructured":"Lipsker D, Ziylan U, Spehner D, Proamer F, Bausinger H, Jeannin P et al (2002) Heat shock proteins 70 and 60 share common receptors which are expressed on human monocyte-derived but not epidermal dendritic cells. Eur J Immunol 32(2):322\u2013332","journal-title":"Eur J Immunol"},{"issue":"8","key":"680_CR26","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1084\/jem.20022194","volume":"198","author":"MW Hornef","year":"2003","unstructured":"Hornef MW, Normark BH, Vandewalle A, Normark S (2003) Intracellular recognition of lipopolysaccharide by toll-like receptor 4 in intestinal epithelial cells. J Exp Med 198(8):1225\u20131235","journal-title":"J Exp Med"},{"issue":"7","key":"680_CR27","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1038\/s41419-022-05048-5","volume":"13","author":"Z Wu","year":"2022","unstructured":"Wu Z, Xu Z, Zhou X, Li H, Zhao L, Lv Y et al (2022) sGRP78 enhances selective autophagy of monomeric TLR4 to regulate myeloid cell death. Cell Death Dis 13(7):587","journal-title":"Cell Death Dis"},{"key":"680_CR28","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.exger.2019.01.016","volume":"118","author":"M Ferraro","year":"2019","unstructured":"Ferraro M, Di Vincenzo S, Dino P, Bucchieri S, Cipollina C, Gjomarkaj M et al (2019) Budesonide, aclidinium and formoterol in combination limit inflammaging processes in bronchial epithelial cells exposed to cigarette smoke. Exp Gerontol 118:78\u201387","journal-title":"Exp Gerontol"},{"issue":"6","key":"680_CR29","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1007\/s12192-015-0618-8","volume":"20","author":"B Qu","year":"2015","unstructured":"Qu B, Jia Y, Liu Y, Wang H, Ren G, Wang H (2015) The detection and role of heat shock protein 70 in various nondisease conditions and disease conditions: a literature review. Cell Stress Chaperones 20(6):885\u2013892","journal-title":"Cell Stress Chaperones"},{"issue":"3","key":"680_CR30","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1007\/s12192-015-0572-5","volume":"20","author":"X Cui","year":"2015","unstructured":"Cui X, Xing J, Liu Y, Zhou Y, Luo X, Zhang Z et al (2015) COPD and levels of Hsp70 (HSPA1A) and Hsp27 (HSPB1) in plasma and lymphocytes among coal workers: a case-control study. Cell Stress Chaperones 20(3):473\u2013481","journal-title":"Cell Stress Chaperones"},{"issue":"5","key":"680_CR31","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1007\/s12192-022-01294-w","volume":"27","author":"A Hulina-Tomaskovic","year":"2022","unstructured":"Hulina-Tomaskovic A, Somborac-Bacura A, Grdic Rajkovic M, Hlapcic I, Jonker MR, Heijink IH et al (2022) Extracellular Hsp70 modulates 16HBE cells\u2019 inflammatory responses to cigarette smoke and bacterial components lipopolysaccharide and lipoteichoic acid. Cell Stress Chaperones 27(5):587\u2013597","journal-title":"Cell Stress Chaperones"},{"key":"680_CR32","volume":"32","author":"J Luo","year":"2022","unstructured":"Luo J, Wang Y, Dong X, Wang W, Mu Y, Sun Y et al (2022) miR-642a-5p increases glucocorticoid sensitivity by suppressing the TLR4 signalling pathway in THP-1 cells. Biochem Biophys Rep 32:101356","journal-title":"Biochem Biophys Rep"},{"key":"680_CR33","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.976454","volume":"13","author":"Z Li","year":"2022","unstructured":"Li Z, Hadlich F, Wimmers K, Murani E (2022) Glucocorticoid receptor hypersensitivity enhances inflammatory signaling and inhibits cell cycle progression in porcine PBMCs. Front Immunol 13:976454","journal-title":"Front Immunol"},{"issue":"6","key":"680_CR34","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1055\/s-2007-980191","volume":"39","author":"LM Colli","year":"2007","unstructured":"Colli LM, do Amaral FC, Torres N, de Castro M (2007) Interindividual glucocorticoid sensitivity in young healthy subjects: the role of glucocorticoid receptor alpha and beta isoforms ratio. Horm Metab Res 39(6):425\u2013429","journal-title":"Horm Metab Res"},{"issue":"3","key":"680_CR35","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1016\/j.intimp.2013.09.003","volume":"17","author":"J Dong","year":"2013","unstructured":"Dong J, Guo L, Liao Z, Zhang M, Zhang M, Wang T et al (2013) Increased expression of heat shock protein 70 in chronic obstructive pulmonary disease. Int Immunopharmacol 17(3):885\u2013893","journal-title":"Int Immunopharmacol"},{"key":"680_CR36","first-page":"139","volume":"9","author":"FL Ramos","year":"2014","unstructured":"Ramos FL, Krahnke JS, Kim V (2014) Clinical issues of mucus accumulation in COPD. Int J Chron Obstruct Pulmon Dis 9:139\u2013150","journal-title":"Int J Chron Obstruct Pulmon Dis"},{"issue":"1","key":"680_CR37","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1186\/1465-9921-12-118","volume":"12","author":"KL Raiford","year":"2011","unstructured":"Raiford KL, Park J, Lin KW, Fang S, Crews AL, Adler KB (2011) Mucin granule-associated proteins in human bronchial epithelial cells: the airway goblet cell \u201cgranulome.\u201d Respir Res 12(1):118","journal-title":"Respir Res"},{"issue":"1","key":"680_CR38","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1186\/s12931-022-02198-0","volume":"23","author":"SS Hussain","year":"2022","unstructured":"Hussain SS, Edwards YJK, Libby EF, Stanford D, Byzek SA, Sin DD et al (2022) Comparative transcriptomics in human COPD reveals dysregulated genes uniquely expressed in ferrets. Respir Res 23(1):277","journal-title":"Respir Res"},{"issue":"3","key":"680_CR39","doi-asserted-by":"publisher","first-page":"962","DOI":"10.1182\/blood-2007-01-066027","volume":"110","author":"Y Wang","year":"2007","unstructured":"Wang Y, Chen T, Han C, He D, Liu H, An H et al (2007) Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Blood 110(3):962\u2013971","journal-title":"Blood"},{"issue":"5","key":"680_CR40","doi-asserted-by":"publisher","first-page":"e01492","DOI":"10.1128\/mBio.01492-14","volume":"5","author":"KA Heyl","year":"2014","unstructured":"Heyl KA, Klassert TE, Heinrich A, Muller MM, Klaile E, Dienemann H et al (2014) Dectin-1 is expressed in human lung and mediates the proinflammatory immune response to nontypeable Haemophilus influenzae. mBio 5(5):e01492-01414","journal-title":"mBio"},{"issue":"5","key":"680_CR41","doi-asserted-by":"publisher","first-page":"874","DOI":"10.3390\/biom13050874","volume":"13","author":"S Safi","year":"2023","unstructured":"Safi S, Messner L, Kliebisch M, Eggert L, Ceylangil C, Lennartz P et al (2023) Circulating Hsp70 levels and the immunophenotype of peripheral blood lymphocytes as potential biomarkers for advanced lung cancer and therapy failure after surgery. Biomolecules 13(5):874","journal-title":"Biomolecules"},{"issue":"6","key":"680_CR42","doi-asserted-by":"publisher","first-page":"3085","DOI":"10.21037\/jtd-20-545","volume":"12","author":"Z Liang","year":"2020","unstructured":"Liang Z, Wang F, Zhang D, Long F, Yang Y, Gu W et al (2020) Sputum and serum autoantibody profiles and their clinical correlation patterns in COPD patients with and without eosinophilic airway inflammation. J Thorac Dis 12(6):3085\u20133100","journal-title":"J Thorac Dis"},{"issue":"15","key":"680_CR43","doi-asserted-by":"publisher","first-page":"8072","DOI":"10.3390\/ijms23158072","volume":"23","author":"J Liu","year":"2022","unstructured":"Liu J, Jiang Y, Jiang Z, Feng Y, Zhao R (2022) Distinct patterns of GR transcriptional regulation in liver and muscle of LPS-challenged weaning piglets. Int J Mol Sci 23(15):8072","journal-title":"Int J Mol Sci"}],"container-title":["Lung"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00408-024-00680-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00408-024-00680-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00408-024-00680-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T19:03:28Z","timestamp":1717182208000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00408-024-00680-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,19]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["680"],"URL":"https:\/\/doi.org\/10.1007\/s00408-024-00680-8","relation":{},"ISSN":["0341-2040","1432-1750"],"issn-type":[{"value":"0341-2040","type":"print"},{"value":"1432-1750","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,19]]},"assertion":[{"value":"10 December 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 February 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 April 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no conflict of interest. The funders had no influence on the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The study was conducted in accordance with the Declaration of Helsinki, and approved by the Institutional Ethics Committee (Ethikkommission Nordwest- und Zentralschweiz, EKNZ) protocol code EKNZ 2016-6-01880 and was registered with ISRCTN registry (ISRCTN11017699).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"All volunteers gave written informed consent for the use of one additional biopsy for translational scientific studies.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}}]}}