{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T02:55:41Z","timestamp":1723258541195},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2012,10,30]],"date-time":"2012-10-30T00:00:00Z","timestamp":1351555200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Legal Med"],"published-print":{"date-parts":[[2013,3]]},"DOI":"10.1007\/s00414-012-0787-2","type":"journal-article","created":{"date-parts":[[2012,10,29]],"date-time":"2012-10-29T21:54:57Z","timestamp":1351547697000},"page":"335-344","source":"Crossref","is-referenced-by-count":20,"title":["Apparent versus true gene expression changes of three hypoxia-related genes in autopsy derived tissue and the importance of normalisation"],"prefix":"10.1007","volume":"127","author":[{"given":"Antje","family":"Huth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedikt","family":"Vennemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony","family":"Fracasso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Lutz-Bonengel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marielle","family":"Vennemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,10,30]]},"reference":[{"key":"787_CR1","doi-asserted-by":"crossref","unstructured":"Bustin SA, Benes V, Garson JA et al (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55(4):611\u2013622","DOI":"10.1373\/clinchem.2008.112797"},{"key":"787_CR2","doi-asserted-by":"crossref","unstructured":"Thellin O, Zorzi W, Lakaye B et al (1999) Housekeeping genes as internal standards: use and limits. J Biotechnol 75:291\u2013295","DOI":"10.1016\/S0168-1656(99)00163-7"},{"key":"787_CR3","doi-asserted-by":"crossref","unstructured":"Vandesompele J, De Preter K, Pattyn F et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. BMC Mol Biol. 3:research0034-research0034.11","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"787_CR4","doi-asserted-by":"crossref","unstructured":"Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169\u201319","DOI":"10.1677\/jme.0.0250169"},{"key":"787_CR5","doi-asserted-by":"crossref","unstructured":"Deo SS, Bhagat AR, Shah RN (2011) Genetic variations in nalp1 mRNA expressions in human vitiligo. Indian J Dermatol 56(3):266\u201371","DOI":"10.4103\/0019-5154.82478"},{"key":"787_CR6","doi-asserted-by":"crossref","unstructured":"Mauro MO, Sartori D, Oliveira RJ, Ishii PL, Mantovani MS, Ribeiro LR (2011) Activity of selenium on cell proliferation, cytotoxicity, and apoptosis and on the expression of CASP9, BCL-XL and APC in intestinal adenocarcinoma cells. Mutat Res 715(1\u20132):7\u201312","DOI":"10.1016\/j.mrfmmm.2011.06.015"},{"key":"787_CR7","doi-asserted-by":"crossref","unstructured":"Huggett J, Dheda K, Bustin S, Zumla A (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6(4):279\u2013284","DOI":"10.1038\/sj.gene.6364190"},{"key":"787_CR8","doi-asserted-by":"crossref","unstructured":"Schmittgen TD, Zakrajsek BA (2000) Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative PCR. J Biochem Biophys Methods 46:69\u201381","DOI":"10.1016\/S0165-022X(00)00129-9"},{"key":"787_CR9","doi-asserted-by":"crossref","unstructured":"Valenti MT, Bertoldo F, Dalle Carbonare L et al (2006) The effect of biphosphonates on gene expression: GAPDH as a housekeeping or a new target gene? BMC Cancer 6:49","DOI":"10.1186\/1471-2407-6-49"},{"key":"787_CR10","doi-asserted-by":"crossref","unstructured":"Tanic N, Perovic M, Mladenovic A, Ruzdijic S, Kanazir S (2007) Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus-evaluation by real-time RT-PCR. J Mol Neurosci 32(1):38\u201346","DOI":"10.1007\/s12031-007-0006-7"},{"key":"787_CR11","doi-asserted-by":"crossref","unstructured":"Goidin D, Mamessier A, Staquet MJ, Schmitt D, Berthier-Vergnes O (2001) Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations. Anal Biochem 295(1):17\u201321","DOI":"10.1006\/abio.2001.5171"},{"key":"787_CR12","doi-asserted-by":"crossref","unstructured":"Lupberger J, Kreuzer KA, Baskaynak G, Peters UR, le Coutre P, Schmidt CA (2002) Quantitative analysis of beta-actin, beta-2-microglobulin and porphobilinogen deaminase mRNA and their comparison as control transcripts for RT-PCR. Mol Cell Probes 16:25\u201330","DOI":"10.1006\/mcpr.2001.0392"},{"key":"787_CR13","doi-asserted-by":"crossref","unstructured":"Gorzelniak K, Janke J, Engeli S, Sharma AM (2001) Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes. Horm Metab Res 33(10):625\u2013627","DOI":"10.1055\/s-2001-17911"},{"key":"787_CR14","doi-asserted-by":"crossref","unstructured":"Bonanomi A, Kojic D, Giger B et al (2003) Quantitative cytokine gene expression in human tonsils at excision and during histoculture assessed by standardized and calibrated real-time PCR and novel data processing. J Immunol Methods 283(1\u20132):27\u201343","DOI":"10.1016\/j.jim.2003.08.002"},{"key":"787_CR15","doi-asserted-by":"crossref","unstructured":"Stamova BS, Apperson M, Walker WL et al (2009) Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood. BMC Med Genomics 2:49","DOI":"10.1186\/1755-8794-2-49"},{"key":"787_CR16","doi-asserted-by":"crossref","unstructured":"Meller M, Vadachkoria S, Luthy DA, Williams MA (2005) Evaluation of housekeeping genes in placental comparative expression studies. Placenta 26(8\u20139):601\u2013607","DOI":"10.1016\/j.placenta.2004.09.009"},{"key":"787_CR17","doi-asserted-by":"crossref","unstructured":"Lagnease J, John R, Schweizer H, Ebmeyer U, Keilhoff G (2008) Selection of reference genes for quantitative real-time PCR in rat asphyxial cardiac arrest model. BMC Mol Biol 9:53","DOI":"10.1186\/1471-2199-9-53"},{"key":"787_CR18","doi-asserted-by":"crossref","unstructured":"Kreth S, Heyn J, Grau S, Ketzschmar HA, Egensperger R, Kreth FW (2010) Identification of valid endogenous control genes for determining gene expression in human glioma. Neuro Oncol 12(6):570\u2013579","DOI":"10.1093\/neuonc\/nop072"},{"key":"787_CR19","doi-asserted-by":"crossref","unstructured":"Hsiao LL, Dangond F, Yoshida T et al (2001) A compendium of gene expression in normal human tissues. Physiol Genomics 7(2):97\u2013104","DOI":"10.1152\/physiolgenomics.00040.2001"},{"key":"787_CR20","doi-asserted-by":"crossref","unstructured":"Bas A, Forsberg G, Hammarstr\u00f6m S, Hammarstr\u00f6m M-L (2004) Utility of the housekeeping genes 18SrRNA, \u03b2-actin and glyceraldehyde-3-phosphate-dehydrogenase for normalization in real-time quantitative reverse transcriptase-polymerase chain reaction analysis of gene expression in human T lymphocytes. Scand J of Immunol 59:566\u2013573","DOI":"10.1111\/j.0300-9475.2004.01440.x"},{"key":"787_CR21","doi-asserted-by":"crossref","unstructured":"Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A (2004) Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques, 37(1): 112\u20134, 116, 118\u20139","DOI":"10.2144\/04371RR03"},{"key":"787_CR22","doi-asserted-by":"crossref","unstructured":"Caradec J, Sirab N, Revaud D, Keumeugni C, Loric S (2010) \u201cDesperate house genes\u201d: the dramatic example of hypoxia. Br J Cancer 102:1037\u20131043","DOI":"10.1038\/sj.bjc.6605573"},{"key":"787_CR23","doi-asserted-by":"crossref","unstructured":"Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64(15):5245\u20135250","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"787_CR24","doi-asserted-by":"crossref","unstructured":"Jiang BH, Semenza GL, Bauer C, Marti HH (1996) Hypoxia-inducible factor 1 levels vary exponentially over a physiologically relevant range of O2 tension. Am J Physiol 271(4 Pt 1):C1172\u201380","DOI":"10.1152\/ajpcell.1996.271.4.C1172"},{"key":"787_CR25","unstructured":"Ozaki H, Yu AY, Della N, Ozaki K et al (1999) Hypoxia inducible factor-1alpha is increased in ischemic retina: temporal and spatial correlation with VEGF expression. Invest Ophthalmol Vis Sci 40(1):182\u20139"},{"key":"787_CR26","doi-asserted-by":"crossref","unstructured":"Zhao D, Zhu BL, Ishikawa T, Li DR, Michiue T, Maeda H (2006) Quantitative RT-PCR assays of hypoxia-inducible factor-1alpha, erythropoietin and vascular endothelial growth factor mRNA transcripts in the kidneys with regard to the cause of death in medicolegal autopsy. Leg Med (Tokyo) 8(5):258\u201363","DOI":"10.1016\/j.legalmed.2006.05.004"},{"key":"787_CR27","doi-asserted-by":"crossref","unstructured":"Zhao D, Zhu BL, Ishikawa T, Quan L, Li DR, Maeda H (2006) Real-time RT-PCR quantitative assays and postmortem degradation profiles of erythropoietin, vascular endothelial growth factor and hypoxia-inducible factor 1 alpha mRNA transcripts in forensic autopsy materials. Leg Med (Tokyo) 8(2):132\u20136","DOI":"10.1016\/j.legalmed.2005.09.001"},{"key":"787_CR28","doi-asserted-by":"crossref","unstructured":"Zhao D, Ishikawa T, Quan L et al (2008) Tissue-specific differences in mRNA quantification of glucose transporter 1 and vascular endothelial growth factor with special regard to death investigations of fatal injuries. Forensic Sci Int 177(2\u20133):176\u201383","DOI":"10.1016\/j.forsciint.2007.12.004"},{"key":"787_CR29","doi-asserted-by":"crossref","unstructured":"Zhu BL, Tanaka S, Ishikawa T et al (2008) Forensic pathological investigation of myocardial hypoxia-inducible factor-1 alpha, erythropoietin and vascular endothelial growth factor in cardiac death. Leg Med (Tokyo) 10(1):11\u20139","DOI":"10.1016\/j.legalmed.2007.06.002"},{"key":"787_CR30","unstructured":"Brahimi Horn C, Pouss\u00e9gur J (2006) The role of the hypoxia-inducible factor in tumor metabolism growth and invasion. Bull Cancer 93(8):E73\u201380"},{"key":"787_CR31","doi-asserted-by":"crossref","unstructured":"Hirota K, Semenza GL (2005) Regulation of hypoxia-inducible factor 1 by prolyl and asparaginyl hydroxylases. Biochem Biophys Res Commun 338(1):610\u2013616","DOI":"10.1016\/j.bbrc.2005.08.193"},{"key":"787_CR32","doi-asserted-by":"crossref","unstructured":"Tai TC, Wong-Faull DC, Claycomb R, Wong DL (2009) Hypoxic stress-induced changes in adrenergic function: role of HIF1 alpha. J Neurochem 109(2):513\u2013524","DOI":"10.1111\/j.1471-4159.2009.05978.x"},{"key":"787_CR33","doi-asserted-by":"crossref","unstructured":"Huang LE, Bunn HF (2003) Hypoxia-inducible factor and its biomedical relevance. J Biol Chem 278(22):19575\u201319578","DOI":"10.1074\/jbc.R200030200"},{"key":"787_CR34","doi-asserted-by":"crossref","unstructured":"Semenza GL (2007) Hypoxia-inducible factor 1 (HIF1) pathway. Sci STKE. 2007(407): cm8","DOI":"10.1126\/stke.4072007cm8"},{"key":"787_CR35","doi-asserted-by":"crossref","unstructured":"Burrows N, Babur M, Resch J, Williams KJ, Brabant G (2011) Hypoxia-inducible factor in thyroid carcinoma. J Thyroid Res 2011:762905","DOI":"10.4061\/2011\/762905"},{"key":"787_CR36","doi-asserted-by":"crossref","unstructured":"Liu LX, Lu H, Luo Y et al (2002) Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1. Biochem Biophys Res Commun 291(4):908\u2013914","DOI":"10.1006\/bbrc.2002.6551"},{"key":"787_CR37","doi-asserted-by":"crossref","unstructured":"Loor G, Schumacker PT (2008) Role of hypoxia-inducible factor in cell survival during myocardial ischemia\u2013reperfusion. Cell Death Differ 15(4):686\u2013690","DOI":"10.1038\/cdd.2008.13"},{"key":"787_CR38","doi-asserted-by":"crossref","unstructured":"Tan SC, Carr CA, Yeoh KK, Schofield CJ, Davies KE, Clarke K (2012) Identification of valid housekeeping genes for quantitative RT-PCR analysis of cardiosphere-derived cells preconditioned under hypoxia or with prolyl-4-hydroxylase inhibitors. Mol Biol Rep 39(4):4857\u201367","DOI":"10.1007\/s11033-011-1281-5"},{"key":"787_CR39","doi-asserted-by":"crossref","unstructured":"Zhong H, Simons JW (1999) Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Biochem Biophys Res Commun 259(3):523\u20136","DOI":"10.1006\/bbrc.1999.0815"},{"key":"787_CR40","doi-asserted-by":"crossref","unstructured":"Said HM, Hagemann C, Stojic J (2007) GAPDH is not regulated in human glioblastoma under hypoxic conditions. BMC Mol Biol 8:55","DOI":"10.1186\/1471-2199-8-55"},{"key":"787_CR41","doi-asserted-by":"crossref","unstructured":"Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8(2):R19","DOI":"10.1186\/gb-2007-8-2-r19"},{"key":"787_CR42","doi-asserted-by":"crossref","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and 2\u2212\u0394\u0394Ct method. Methods 25(4):402\u2013408","DOI":"10.1006\/meth.2001.1262"},{"key":"787_CR43","doi-asserted-by":"crossref","unstructured":"Koppelkamm A, Vennemann B, Fracasso T, Lutz-Bonengel S, Schmidt U, Heinrich M (2010) Validation of adequate endogenous reference genes for the normalisation of qPCR gene expression data in human post mortem tissue. Int J Leg Med 124(5):371\u2013380","DOI":"10.1007\/s00414-010-0433-9"},{"key":"787_CR44","doi-asserted-by":"crossref","unstructured":"Koppelkamm A, Vennemann B, Lutz-Bonengel S, Fracasso T, Vennemann M (2011) RNA integrity in post-mortem samples: influencing parameters and implications on RT-qPCR assays. Int J Legal Med 125(4):573\u2013580","DOI":"10.1007\/s00414-011-0578-1"},{"key":"787_CR45","doi-asserted-by":"crossref","unstructured":"Heinrich M, Matt K, Lutz-Bonengel S (2007) Schmidt U (2007) Successful RNA extraction from various human postmortem tissues. Int J Legal Med 121(2):136\u201342","DOI":"10.1007\/s00414-006-0131-9"},{"key":"787_CR46","doi-asserted-by":"crossref","unstructured":"Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45","DOI":"10.1093\/nar\/29.9.e45"},{"key":"787_CR47","doi-asserted-by":"crossref","unstructured":"Jang M, Kang HJ, Lee SY et al (2009) Glyceraldehyde-3-phosphate, a glycolytic intermediate, plays a key role in controlling cell fate via inhibition of caspase activity. Mol Cells 28(6):559\u201363","DOI":"10.1007\/s10059-009-0151-7"},{"key":"787_CR48","doi-asserted-by":"crossref","unstructured":"Higashimura Y, Nakajima Y, Yamaji R et al (2011) Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells. Arch Biochem Biophys 509:1\u20138","DOI":"10.1016\/j.abb.2011.02.011"},{"key":"787_CR49","doi-asserted-by":"crossref","unstructured":"Tricarico C, Pinzani P, Bianchi S et al (2002) Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem 309(2):293\u2013300","DOI":"10.1016\/S0003-2697(02)00311-1"},{"key":"787_CR50","doi-asserted-by":"crossref","unstructured":"Lu S, Gu X, Hoestje S (2002) Epner DE (2002) Identification of an additional hypoxia responsive element in the glyceraldehyde-3-phosphate dehydrogenase gene promoter. Biochim Biophys Acta 1574(2):152\u20136","DOI":"10.1016\/S0167-4781(01)00359-1"}],"container-title":["International Journal of Legal Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00414-012-0787-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00414-012-0787-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00414-012-0787-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T21:33:36Z","timestamp":1714512816000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00414-012-0787-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,30]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["787"],"URL":"https:\/\/doi.org\/10.1007\/s00414-012-0787-2","relation":{},"ISSN":["0937-9827","1437-1596"],"issn-type":[{"value":"0937-9827","type":"print"},{"value":"1437-1596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,30]]}}}