{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T04:09:46Z","timestamp":1744862986405,"version":"3.40.4"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Int J Legal Med"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1007\/s00414-024-03400-2","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T01:48:47Z","timestamp":1734313727000},"page":"1105-1112","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression of RIPK-1 and S-100B in traumatic brain injury\u2013 exploring a forensic cases series"],"prefix":"10.1007","volume":"139","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5248-6896","authenticated-orcid":false,"given":"Gabriele","family":"Napoletano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Marinelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Palla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Zaami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aniello","family":"Maiese","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"3400_CR1","doi-asserted-by":"publisher","first-page":"1080","DOI":"10.3171\/2017.10.JNS17352","volume":"130","author":"MC Dewan","year":"2018","unstructured":"Dewan MC, Rattani A, Gupta S, Baticulon RE, Hung Y-C, Punchak M, Agrawal A, Adeleye AO, Shrime MG, Rubiano AM et al (2018) Estimating the global incidence of traumatic brain injury. J Neurosurg 130:1080\u20131097. https:\/\/doi.org\/10.3171\/2017.10.JNS17352","journal-title":"J Neurosurg"},{"key":"3400_CR2","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1111\/j.1365-2990.2006.00794.x","volume":"33","author":"T Hortob\u00e1gyi","year":"2007","unstructured":"Hortob\u00e1gyi T, Wise S, Hunt N, Cary N, Djurovic V, Fegan-Earl A, Shorrock K, Rouse D, Al-Sarraj S (2007) Traumatic axonal damage in the brain can be detected using beta-APP immunohistochemistry within 35 min after head injury to human adults. Neuropathol Appl Neurobiol 33:226\u2013237. https:\/\/doi.org\/10.1111\/j.1365-2990.2006.00794.x","journal-title":"Neuropathol Appl Neurobiol"},{"key":"3400_CR3","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1007\/s00414-022-02807-z","volume":"136","author":"S Al-Sarraj","year":"2022","unstructured":"Al-Sarraj S, Troakes C, Rutty GN (2022) Axonal injury is detected by \u03b2APP immunohistochemistry in rapid death from head injury following road traffic collision. Int J Legal Med 136:1321\u20131339. https:\/\/doi.org\/10.1007\/s00414-022-02807-z","journal-title":"Int J Legal Med"},{"key":"3400_CR4","doi-asserted-by":"publisher","first-page":"2167","DOI":"10.1007\/s00414-020-02384-z","volume":"134","author":"S Bohnert","year":"2020","unstructured":"Bohnert S, Seiffert A, Trella S, Bohnert M, Distel L, Ondruschka B, Monoranu C.-M. (2020) TMEM119 as a specific marker of microglia reaction in traumatic brain injury in postmortem examination. Int J Legal Med 134:2167\u20132176. https:\/\/doi.org\/10.1007\/s00414-020-02384-z","journal-title":"Int J Legal Med"},{"key":"3400_CR5","doi-asserted-by":"publisher","first-page":"13651","DOI":"10.1038\/s41598-024-64312-0","volume":"14","author":"S Bohnert","year":"2024","unstructured":"Bohnert S, Reinert C, Trella S, Cattaneo A, Prei\u00df U, Bohnert M, Zwirner J, B\u00fcttner A, Schmitz W, Ondruschka B (2024) Neuroforensomics: metabolites as valuable biomarkers in cerebrospinal fluid of lethal traumatic brain injuries. Sci Rep 14:13651. https:\/\/doi.org\/10.1038\/s41598-024-64312-0","journal-title":"Sci Rep"},{"key":"3400_CR6","doi-asserted-by":"publisher","first-page":"795","DOI":"10.14670\/HH-18-319","volume":"36","author":"M dell\u2019Aquila","year":"2021","unstructured":"dell\u2019Aquila M, Maiese A, De Matteis A, Viola RV, Arcangeli M, La Russa R, Fineschi V (2021) Traumatic brain injury: estimate of the age of the injury based on neuroinflammation, endothelial activation markers and adhesion molecules. Histol Histopathol 36:795\u2013806. https:\/\/doi.org\/10.14670\/HH-18-319","journal-title":"Histol Histopathol"},{"key":"3400_CR7","unstructured":"Adams and Victor\u2019s Principles of Neurology, 11e| AccessNeurology| McGraw Hill Medical Available online: https:\/\/neurology.mhmedical.com\/content.aspx?bookid=1477&sectionid=85536145 (accessed on 11 June 2024)"},{"key":"3400_CR8","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.jflm.2011.12.015","volume":"19","author":"S Al-Sarraj","year":"2012","unstructured":"Al-Sarraj S, Fegan-Earl A, Ugbade A, Bodi I, Chapman R, Poole S, Swift B, Jerreat P, Cary N (2012) Focal traumatic brain stem injury is a rare type of head injury resulting from assault: a forensic neuropathological study. J Forensic Leg Med 19:144\u2013151. https:\/\/doi.org\/10.1016\/j.jflm.2011.12.015","journal-title":"J Forensic Leg Med"},{"key":"3400_CR9","doi-asserted-by":"publisher","unstructured":"Neri M, B\u00fcttner A, Fineschi V (2017) Brain injury due to mechanical trauma and ischemic-hypoxic insult: biomarkers of brain injury and oxidative stress. Oxid Med Cell Longev 2017:8923472, https:\/\/doi.org\/10.1155\/2017\/8923472","DOI":"10.1155\/2017\/8923472"},{"key":"3400_CR10","doi-asserted-by":"publisher","first-page":"10865","DOI":"10.3390\/ijms221910865","volume":"22","author":"M Palmieri","year":"2021","unstructured":"Palmieri M, Frati A, Santoro A, Frati P, Fineschi V, Pesce A (2021) Diffuse axonal injury: clinical prognostic factors, molecular experimental models and the impact of the trauma related oxidative stress. An extensive review concerning milestones and advances. Int J Mol Sci 22:10865. https:\/\/doi.org\/10.3390\/ijms221910865","journal-title":"Int J Mol Sci"},{"key":"3400_CR11","doi-asserted-by":"publisher","unstructured":"Frati A, Cerretani D, Fiaschi AI, Frati P, Gatto V, La Russa R, Pesce A, Pinchi E, Santurro A, Fraschetti F et al (2017) Diffuse axonal injury and oxidative stress: a comprehensive review. Int J Mol Sci 18. https:\/\/doi.org\/10.3390\/ijms18122600","DOI":"10.3390\/ijms18122600"},{"key":"3400_CR12","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1007\/s00414-006-0126-6","volume":"121","author":"J Dressler","year":"2007","unstructured":"Dressler J, Hanisch U, Kuhlisch E, Geiger KD (2007) Neuronal and glial apoptosis in human traumatic brain injury. Int J Legal Med 121:365\u2013375. https:\/\/doi.org\/10.1007\/s00414-006-0126-6","journal-title":"Int J Legal Med"},{"key":"3400_CR13","doi-asserted-by":"publisher","first-page":"2584","DOI":"10.3390\/ijms24032584","volume":"24","author":"A Maiese","year":"2023","unstructured":"Maiese A, Spina F, Visi G, Del Duca F, De Matteis A, La Russa R, Di Paolo M, Frati P, Fineschi V (2023) The expression of foxo3a as a forensic diagnostic tool in cases of traumatic brain injury: an immunohistochemical study. Int J Mol Sci 24:2584. https:\/\/doi.org\/10.3390\/ijms24032584","journal-title":"Int J Mol Sci"},{"key":"3400_CR14","doi-asserted-by":"publisher","first-page":"1862","DOI":"10.1089\/neu.2013.2895","volume":"30","author":"B Ondruschka","year":"2013","unstructured":"Ondruschka B, Pohlers D, Sommer G, Schober K, Teupser D, Franke H, Dressler J (2013) S100B and NSE as useful postmortem biochemical markers of traumatic brain injury in autopsy cases. J Neurotrauma 30:1862\u20131871. https:\/\/doi.org\/10.1089\/neu.2013.2895","journal-title":"J Neurotrauma"},{"key":"3400_CR15","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.legalmed.2011.12.007","volume":"14","author":"D-R Li","year":"2012","unstructured":"Li D-R, Zhang F, Wang Y, Tan X-H, Qiao D-F, Wang H-J, Michiue T, Maeda H (2012) Quantitative analysis of GFAP- and S100 protein-immunopositive astrocytes to investigate the severity of traumatic brain injury. Leg Med (Tokyo) 14:84\u201392. https:\/\/doi.org\/10.1016\/j.legalmed.2011.12.007","journal-title":"Leg Med (Tokyo)"},{"key":"3400_CR16","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1089\/neu.2014.3524","volume":"32","author":"M Krohn","year":"2015","unstructured":"Krohn M, Dre\u00dfler J, Bauer M, Schober K, Franke H, Ondruschka B (2015) Immunohistochemical investigation of S100 and NSE in cases of traumatic brain injury and its application for survival time determination. J Neurotrauma 32:430\u2013440. https:\/\/doi.org\/10.1089\/neu.2014.3524","journal-title":"J Neurotrauma"},{"key":"3400_CR17","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.legalmed.2005.09.002","volume":"8","author":"D-R Li","year":"2006","unstructured":"Li D-R, Zhu B-L, Ishikawa T, Zhao D, Michiue T, Maeda H (2006) Immunohistochemical distribution of S-100 protein in the cerebral cortex with regard to the cause of death in forensic autopsy. Leg Med (Tokyo) 8:78\u201385. https:\/\/doi.org\/10.1016\/j.legalmed.2005.09.002","journal-title":"Leg Med (Tokyo)"},{"key":"3400_CR18","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.jare.2021.12.002","volume":"40","author":"X Hu","year":"2022","unstructured":"Hu X, Xu Y, Zhang H, Li Y, Wang X, Xu C, Ni W, Zhou K (2022) Role of necroptosis in traumatic brain and spinal cord injuries. J Adv Res 40:125\u2013134. https:\/\/doi.org\/10.1016\/j.jare.2021.12.002","journal-title":"J Adv Res"},{"key":"3400_CR19","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1186\/s40478-021-01236-0","volume":"9","author":"AC Wehn","year":"2021","unstructured":"Wehn AC, Khalin I, Duering M, Hellal F, Culmsee C, Vandenabeele P, Plesnila N, Terpolilli NA (2021) RIPK1 or RIPK3 deletion prevents progressive neuronal cell death and improves memory function after traumatic brain injury. Acta Neuropathol Commun 9:138. https:\/\/doi.org\/10.1186\/s40478-021-01236-0","journal-title":"Acta Neuropathol Commun"},{"key":"3400_CR20","doi-asserted-by":"publisher","first-page":"24547","DOI":"10.1038\/srep24547","volume":"6","author":"T Liu","year":"2016","unstructured":"Liu T, Zhao D, Cui H, Chen L, Bao Y, Wang Y, Jiang J (2016) Therapeutic hypothermia attenuates tissue damage and cytokine expression after traumatic brain injury by inhibiting necroptosis in the rat. Sci Rep 6:24547. https:\/\/doi.org\/10.1038\/srep24547","journal-title":"Sci Rep"},{"key":"3400_CR21","doi-asserted-by":"publisher","first-page":"9605","DOI":"10.3390\/ijms24119605","volume":"24","author":"F Michetti","year":"2023","unstructured":"Michetti F, Clementi ME, Di Liddo R, Valeriani F, Ria F, Rende M, Di Sante G, Romano Spica, V. (2023) The S100B protein: a multifaceted pathogenic factor more than a biomarker. Int J Mol Sci 24:9605. https:\/\/doi.org\/10.3390\/ijms24119605","journal-title":"Int J Mol Sci"},{"key":"3400_CR22","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1002\/jnr.21211","volume":"85","author":"J Sen","year":"2007","unstructured":"Sen J, Belli A (2007) S100B in neuropathologic states: the CRP of the brain? J Neurosci Res 85:1373\u20131380. https:\/\/doi.org\/10.1002\/jnr.21211","journal-title":"J Neurosci Res"},{"key":"3400_CR23","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.legalmed.2005.07.004","volume":"8","author":"D-R Li","year":"2006","unstructured":"Li D-R, Zhu B-L, Ishikawa T, Zhao D, Michiue T, Maeda H (2006) Postmortem serum protein s100b levels with regard to the cause of death involving brain damage in medicolegal autopsy cases. Leg Med 8:71\u201377. https:\/\/doi.org\/10.1016\/j.legalmed.2005.07.004","journal-title":"Leg Med"},{"key":"3400_CR24","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1007\/s00414-022-02785-2","volume":"136","author":"J Zwirner","year":"2022","unstructured":"Zwirner J, Kulakofsky R, Fitzek A, Schr\u00f6der AS, Bohnert S, Franke H, Renn\u00e9 T, Tse R, Ondruschka B (2022) Forensic biomarkers of lethal traumatic brain injury. Int J Legal Med 136:871\u2013886. https:\/\/doi.org\/10.1007\/s00414-022-02785-2","journal-title":"Int J Legal Med"},{"key":"3400_CR25","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1016\/j.neubiorev.2021.04.035","volume":"127","author":"F Michetti","year":"2021","unstructured":"Michetti F, Di Sante G, Clementi ME, Sampaolese B, Casalbore P, Volont\u00e9 C, Romano Spica V, Parnigotto PP, Di Liddo R, Amadio S et al (2021) Growing role of S100B protein as a putative therapeutic target for neurological- and nonneurological-disorders. Neurosci Biobehav Rev 127:446\u2013458. https:\/\/doi.org\/10.1016\/j.neubiorev.2021.04.035","journal-title":"Neurosci Biobehav Rev"},{"key":"3400_CR26","doi-asserted-by":"publisher","first-page":"265","DOI":"10.2174\/1570159X18666200729100427","volume":"19","author":"U Langeh","year":"2021","unstructured":"Langeh U, Singh S, Targeting (2021) S100B protein as a surrogate biomarker and its role in various neurological disorders. Curr Neuropharmacol 19:265\u2013277. https:\/\/doi.org\/10.2174\/1570159X18666200729100427","journal-title":"Curr Neuropharmacol"},{"key":"3400_CR27","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.jflm.2018.02.016","volume":"55","author":"M Sieber","year":"2018","unstructured":"Sieber M, Dre\u00dfler J, Franke H, Pohlers D, Ondruschka B (2018) Post-mortem biochemistry of NSE and S100B: a supplemental tool for detecting a lethal traumatic brain injury? J Forensic Leg Med 55:65\u201373. https:\/\/doi.org\/10.1016\/j.jflm.2018.02.016","journal-title":"J Forensic Leg Med"},{"key":"3400_CR28","doi-asserted-by":"publisher","first-page":"1443","DOI":"10.1002\/glia.22533","volume":"61","author":"L Nobs","year":"2013","unstructured":"Nobs L, Nestel S, Kulik A, Nitsch C, Atanasoski S (2013) Cyclin D1 is required for proliferation of Olig2-expressing progenitor cells in the injured cerebral cortex. Glia 61:1443\u20131455. https:\/\/doi.org\/10.1002\/glia.22533","journal-title":"Glia"},{"key":"3400_CR29","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1038\/cdd.2008.150","volume":"16","author":"G Kroemer","year":"2009","unstructured":"Kroemer G, Galluzzi L, Vandenabeele P, Abrams J, Alnemri ES, Baehrecke EH, Blagosklonny MV, El-Deiry WS, Golstein P, Green DR et al (2009) Classification of cell death: recommendations of the nomenclature committee on cell death 2009. Cell Death Differ 16:3\u201311. https:\/\/doi.org\/10.1038\/cdd.2008.150","journal-title":"Cell Death Differ"},{"key":"3400_CR30","doi-asserted-by":"publisher","first-page":"1114103","DOI":"10.3389\/fimmu.2023.1114103","volume":"14","author":"X Liu","year":"2023","unstructured":"Liu X, Tang A-L, Cheng J, Gao N, Zhang G, Xiao C (2023) RIPK1 in the inflammatory response and sepsis: recent advances, drug discovery and beyond. Front Immunol 14:1114103. https:\/\/doi.org\/10.3389\/fimmu.2023.1114103","journal-title":"Front Immunol"},{"key":"3400_CR31","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1038\/cdd.2011.164","volume":"19","author":"N Vanlangenakker","year":"2012","unstructured":"Vanlangenakker N, Vanden Berghe T, Vandenabeele P (2012) Many stimuli pull the necrotic trigger, an overview. Cell Death Differ 19:75\u201386. https:\/\/doi.org\/10.1038\/cdd.2011.164","journal-title":"Cell Death Differ"},{"key":"3400_CR32","doi-asserted-by":"publisher","unstructured":"Bao Z, Fan L, Zhao L, Xu X, Liu Y, Chao H, Liu N, You Y, Liu Y, Wang X et al (2019) Silencing of A20 aggravates neuronal death and inflammation after traumatic brain injury: a potential trigger of necroptosis. Front Mol Neurosci 12. https:\/\/doi.org\/10.3389\/fnmol.2019.00222","DOI":"10.3389\/fnmol.2019.00222"},{"key":"3400_CR33","doi-asserted-by":"publisher","first-page":"674","DOI":"10.3389\/fnins.2019.00674","volume":"13","author":"H Ni","year":"2019","unstructured":"Ni H, Rui Q, Lin X, Li D, Liu H, Chen G (2019) 2-BFI provides neuroprotection against inflammation and necroptosis in a rat model of traumatic brain injury. Front Neurosci 13:674. https:\/\/doi.org\/10.3389\/fnins.2019.00674","journal-title":"Front Neurosci"},{"key":"3400_CR34","doi-asserted-by":"publisher","first-page":"263310552090640","DOI":"10.1177\/2633105520906402","volume":"15","author":"H Kanno","year":"2020","unstructured":"Kanno H, Ozawa H, Handa K, Murakami T, Itoi E (2020) Changes in expression of receptor-interacting protein kinase 1 in secondary neural tissue damage following spinal cord injury. J Exp Neurosci 15:2633105520906402. https:\/\/doi.org\/10.1177\/2633105520906402","journal-title":"J Exp Neurosci"},{"key":"3400_CR35","doi-asserted-by":"publisher","unstructured":"Hagmeyer S, Rom\u00e3o MA, Crist\u00f3v\u00e3o JS, Vilella A, Zoli M, Gomes CM, Grabrucker AM (2019) Distribution and relative abundance of S100 proteins in the brain of the APP23 Alzheimer\u2019s disease model mice. Front Neurosci 13. https:\/\/doi.org\/10.3389\/fnins.2019.00640","DOI":"10.3389\/fnins.2019.00640"},{"key":"3400_CR36","doi-asserted-by":"publisher","first-page":"463","DOI":"10.3389\/fnins.2019.00463","volume":"13","author":"JS Crist\u00f3v\u00e3o","year":"2019","unstructured":"Crist\u00f3v\u00e3o JS, Gomes CM (2019) S100 proteins in Alzheimer\u2019s disease. Front Neurosci 13:463. https:\/\/doi.org\/10.3389\/fnins.2019.00463","journal-title":"Front Neurosci"},{"key":"3400_CR37","doi-asserted-by":"publisher","first-page":"1236","DOI":"10.1038\/nn.4608","volume":"20","author":"A Caccamo","year":"2017","unstructured":"Caccamo A, Branca C, Piras IS, Ferreira E, Huentelman MJ, Liang WS, Readhead B, Dudley JT, Spangenberg EE, Green KN et al (2017) Necroptosis activation in Alzheimer\u2019s disease. Nat Neurosci 20:1236\u20131246. https:\/\/doi.org\/10.1038\/nn.4608","journal-title":"Nat Neurosci"}],"container-title":["International Journal of Legal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00414-024-03400-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00414-024-03400-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00414-024-03400-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T12:08:53Z","timestamp":1744805333000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00414-024-03400-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,16]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["3400"],"URL":"https:\/\/doi.org\/10.1007\/s00414-024-03400-2","relation":{},"ISSN":["0937-9827","1437-1596"],"issn-type":[{"type":"print","value":"0937-9827"},{"type":"electronic","value":"1437-1596"}],"subject":[],"published":{"date-parts":[[2024,12,16]]},"assertion":[{"value":"18 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"N\/A.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"N\/A.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"N\/A.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no conflicts of interest.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}