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However, inter- and intra-observer variability can affect DAE reliability, particularly when evaluating panoramic radiographs influenced by magnification, distortion, and anatomical superimposition. In the staging process, consciously or unconsciously, experts analyze dental development within the available peripheral anatomic features of the tooth and its peripheral region. This study investigated whether isolating teeth as regions of interest (cropped images) influences the reproducibility of Demirjian\u2019s stage allocation. An observational cross-sectional study was conducted using 301 panoramic radiographs from subjects aged 7\u201314 years. Three experienced forensic odontologists independently staged all seven left mandibular teeth (#31\u2013#37) and, when present, also the left mandibular third molar (#38) using the Demirjian\u2019s staging system. Each tooth was evaluated twice, two weeks apart, in both full panoramic radiograph and cropped formats. Inter- and intra-observer agreement was assessed using kappa statistics. Intra-observer agreement was above 0.81. Cropped images resulted in only marginal changes, with a slight decrease in stage reproducibility (\u0394\u2009\u2212\u20090.017 and \u2212\u20090.023). Inter-observer agreement was nearly identical between non-cropped (AC2\u2009=\u20090.985) and cropped images (AC2\u2009=\u20090.986). A slightly lower proportion of cropped images was assessable by all observers (90.7% vs. 91.7%). Isolating teeth as regions of interest did not meaningfully reduce reproducibility and only small intra-observer decreases were observed and concentrated in the anterior teeth. These findings indicate that, among experienced observers, cropping does not degrade the reliability of Demirjian stage allocation. Future DAE staging systems should consider being more descriptive, including not only tooth-related developmental details, but surrounding information, such as the distance between the analyzed tooth and the occlusal plane and its relationship with adjacent developed teeth.<\/jats:p>","DOI":"10.1007\/s00414-026-03929-4","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T01:46:49Z","timestamp":1784771209000},"page":"4483-4493","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The influence of adjacent anatomical context in tooth staging: observational study of non-cropped vs. cropped radiographs for dental age estimation"],"prefix":"10.1007","volume":"140","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8511-4645","authenticated-orcid":false,"given":"Nikolaos","family":"Angelakopoulos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5045-6773","authenticated-orcid":false,"given":"Rizky Merdietio","family":"Boedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1725-0853","authenticated-orcid":false,"given":"Stefano","family":"De Luca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2603-5950","authenticated-orcid":false,"given":"Israel Soriano","family":"V\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1417-2781","authenticated-orcid":false,"given":"Ademir","family":"Franco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"3929_CR1","doi-asserted-by":"publisher","first-page":"1881","DOI":"10.1007\/s00414-024-03254-8","volume":"138","author":"F Thicot","year":"2024","unstructured":"Thicot F, Egger C, Castiglioni C et al (2024) Forensic age estimation at the University Center of Legal Medicine Lausanne-Geneva: a retrospective study over 12 years. 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