{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T18:39:55Z","timestamp":1792435195904,"version":"4.5.0"},"reference-count":103,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82502274"],"award-info":[{"award-number":["82502274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Sichuan Postdoctoral Research Project Special Funding","award":["TB2025017"],"award-info":[{"award-number":["TB2025017"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Int J Legal Med"],"DOI":"10.1007\/s00414-026-04046-y","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T01:07:57Z","timestamp":1792372077000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single-cell strategies for the interpretation of complex forensic DNA mixtures: isolation techniques, genotype data integration, and prospects for application"],"prefix":"10.1007","author":[{"given":"Ruijia","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3419-1741","authenticated-orcid":false,"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1356-0073","authenticated-orcid":false,"given":"Jing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"issue":"11","key":"4046_CR1","doi-asserted-by":"publisher","first-page":"107961","DOI":"10.1016\/j.isci.2023.107961","volume":"26","author":"K Huffman","year":"2023","unstructured":"Huffman K, Ballantyne J (2023) Single cell genomics applications in forensic science: Current state and future directions. iScience 26(11):107961. https:\/\/doi.org\/10.1016\/j.isci.2023.107961","journal-title":"iScience"},{"issue":"6","key":"4046_CR2","doi-asserted-by":"publisher","first-page":"730","DOI":"10.13618\/j.issn.1001-5728.2024.06.017","volume":"39","author":"AQ Chen","year":"2024","unstructured":"Chen AQ, Zhang SH (2024) Role of high-throughput sequencing technology: a key tool in resolving forensic challenges in biological sample identification. Chin J Forensic Med 39(6):730\u2013740. https:\/\/doi.org\/10.13618\/j.issn.1001-5728.2024.06.017","journal-title":"Chin J Forensic Med"},{"key":"4046_CR3","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1007\/s00194-018-0291-1","volume":"29","author":"K Anslinger","year":"2019","unstructured":"Anslinger K, Graw M, Bayer B (2019) Deconvolution of blood-blood mixtures using DEPArray\u2122 separated single cell STR profiling. Rechtsmedizin 29:30\u201340. https:\/\/doi.org\/10.1007\/s00194-018-0291-1","journal-title":"Rechtsmedizin"},{"issue":"7","key":"4046_CR4","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.3390\/genes13071117","volume":"13","author":"K Huffman","year":"2022","unstructured":"Huffman K, Hanson E, Ballantyne J (2022) Cell Subsampling Recovers Probative DNA Profile Information from Unresolvable\/Undetectable Minor Donors in Mixtures. Genes 13(7):1117. https:\/\/doi.org\/10.3390\/genes13071117","journal-title":"Genes"},{"issue":"2","key":"4046_CR5","doi-asserted-by":"publisher","first-page":"176","DOI":"10.16467\/j.1008-3650.2021.0166","volume":"47","author":"Y Shi","year":"2022","unstructured":"Shi Y, Shang JB, Luo PY, Li LR, Zhao Y, Liu YX, Wang BJ, Yao J (2022) Research progress of seminal and vaginal mixed stains about their separation and detection in forensic evidential materials. Forensic Sci Technol 47(2):176\u2013184. https:\/\/doi.org\/10.16467\/j.1008-3650.2021.0166","journal-title":"Forensic Sci Technol"},{"issue":"1","key":"4046_CR6","doi-asserted-by":"publisher","first-page":"7054","DOI":"10.1038\/s41598-021-86271-6","volume":"11","author":"DRL Watkins","year":"2021","unstructured":"Watkins DRL, Myers D, Xavier HE, Marciano MA (2021) Revisiting single cell analysis in forensic science. Sci Rep 11(1):7054. https:\/\/doi.org\/10.1038\/s41598-021-86271-6","journal-title":"Sci Rep"},{"issue":"6","key":"4046_CR7","doi-asserted-by":"publisher","first-page":"1875","DOI":"10.1111\/1556-4029.15344","volume":"68","author":"J Schulte","year":"2023","unstructured":"Schulte J, Marciano MA, Scheurer E, Schulz I (2023) A systematic approach to improve downstream single-cell analysis for the DEPArray\u2122 technology. J Forensic Sci 68(6):1875\u20131893. https:\/\/doi.org\/10.1111\/1556-4029.15344","journal-title":"J Forensic Sci"},{"issue":"1","key":"4046_CR8","doi-asserted-by":"publisher","first-page":"2524","DOI":"10.1038\/s41598-024-83841-2","volume":"15","author":"C Costa","year":"2025","unstructured":"Costa C, Figueiredo C, Costa S, Ferreira PM, Amorim A, Prieto L, Pinto N (2025) The impact of parameter variation in the quantification of forensic genetic evidence. Sci Rep 15(1):2524. https:\/\/doi.org\/10.1038\/s41598-024-83841-2","journal-title":"Sci Rep"},{"issue":"4","key":"4046_CR9","doi-asserted-by":"publisher","first-page":"1813","DOI":"10.1007\/s12024-024-00872-x","volume":"21","author":"AC Minervino","year":"2025","unstructured":"Minervino AC, de Arag\u00e3o Gualda CM, Trindade BR, de Medeiros CEM, da Silva J\u00fanior RC (2025) Reanalyzing DNA mixture: a evaluation of EuroForMix for deconvolution and weight-of-evidence computing. Forensic Sci Med Pathol 21(4):1813\u20131819. https:\/\/doi.org\/10.1007\/s12024-024-00872-x","journal-title":"Forensic Sci Med Pathol"},{"key":"4046_CR10","doi-asserted-by":"publisher","first-page":"112242","DOI":"10.1016\/j.forsciint.2024.112242","volume":"364","author":"S Yamada","year":"2024","unstructured":"Yamada S, Dozen Y, Tatsumi K, Okamoto M, Ikemoto C, Michiue T, Nagai A (2024) Individual identification from mixed-blood spots by using four cells with single-cell genomic analysis. Forensic Sci Int 364:112242. https:\/\/doi.org\/10.1016\/j.forsciint.2024.112242","journal-title":"Forensic Sci Int"},{"issue":"1","key":"4046_CR11","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.fsigss.2007.10.186","volume":"1","author":"C Petit","year":"2008","unstructured":"Petit C, Martel-Petit V, Hienne R, Frackowiak S (2008) Single sperm cell isolation by micromanipulation for human identification in sexual assault. Forensic Sci Int Genet Suppl Ser 1(1):60\u201362. https:\/\/doi.org\/10.1016\/j.fsigss.2007.10.186","journal-title":"Forensic Sci Int Genet Suppl Ser"},{"key":"4046_CR12","doi-asserted-by":"publisher","first-page":"100240","DOI":"10.1016\/j.fsir.2021.100240","volume":"4","author":"GMG Theunissen","year":"2021","unstructured":"Theunissen GMG, Gibb A, Lin PKT, Rolf B, Forat S, J\u00e4ger R (2021) DNA profiling of single sperm cells after whole genome amplification. Forensic Sci Int Rep 4:100240. https:\/\/doi.org\/10.1016\/j.fsir.2021.100240","journal-title":"Forensic Sci Int Rep"},{"key":"4046_CR13","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1007\/978-1-0716-5060-8_4","volume":"3003","author":"GMG Theunissen","year":"2026","unstructured":"Theunissen GMG, J\u00e4ger R (2026) Micromanipulation and Multiple-Strand Displacement Amplification for Forensic DNA Profiling of Single Sperm Cells. Methods Mol Biol 3003:37\u201349. https:\/\/doi.org\/10.1007\/978-1-0716-5060-8_4","journal-title":"Methods Mol Biol"},{"issue":"1","key":"4046_CR14","doi-asserted-by":"publisher","first-page":"29","DOI":"10.13406\/j.cnki.cyxb.002383","volume":"45","author":"RY Wang","year":"2020","unstructured":"Wang RY, Meng Q (2020) Advances in laser capture microdissection and its clinical application. J Chongqing Med Univ 45(1):29\u201331. https:\/\/doi.org\/10.13406\/j.cnki.cyxb.002383","journal-title":"J Chongqing Med Univ"},{"issue":"9","key":"4046_CR15","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.jgg.2023.07.011","volume":"50","author":"W Guo","year":"2023","unstructured":"Guo W, Hu Y, Qian J, Zhu L, Cheng J, Liao J, Fan X (2023) Laser capture microdissection for biomedical research: towards high-throughput, multi-omics, and single-cell resolution. J Genet Genomics 50(9):641\u2013651. https:\/\/doi.org\/10.1016\/j.jgg.2023.07.011","journal-title":"J Genet Genomics"},{"issue":"7","key":"4046_CR16","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1016\/S0168-9525(98)01489-9","volume":"14","author":"NL Simone","year":"1998","unstructured":"Simone NL, Bonner RF, Gillespie JW, Emmert-Buck MR, Liotta LA (1998) Laser capture microdissection: opening the microscopic frontier to molecular analysis. Trends Genet 14(7):272\u2013276. https:\/\/doi.org\/10.1016\/S0168-9525(98)01489-9","journal-title":"Trends Genet"},{"issue":"4","key":"4046_CR17","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1111\/j.1556-4029.2006.00180.x","volume":"51","author":"CT Sanders","year":"2006","unstructured":"Sanders CT, Sanchez N, Ballantyne J, Peterson DA (2006) Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis. J Forensic Sci 51(4):748\u2013757. https:\/\/doi.org\/10.1111\/j.1556-4029.2006.00180.x","journal-title":"J Forensic Sci"},{"key":"4046_CR18","doi-asserted-by":"publisher","first-page":"102278","DOI":"10.1016\/j.fsigen.2020.102278","volume":"47","author":"R England","year":"2020","unstructured":"England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison S (2020) Compatibility of the ForenSeq\u2122 DNA Signature Prep Kit with laser microdissected cells: An exploration of issues that arise with samples containing low cell numbers. Forensic Sci Int Genet 47:102278. https:\/\/doi.org\/10.1016\/j.fsigen.2020.102278","journal-title":"Forensic Sci Int Genet"},{"issue":"6","key":"4046_CR19","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1007\/s00414-010-0499-4","volume":"124","author":"M Vandewoestyne","year":"2010","unstructured":"Vandewoestyne M, Deforce D (2010) Laser capture microdissection in forensic research: a review. Int J Legal Med 124(6):513\u2013521. https:\/\/doi.org\/10.1007\/s00414-010-0499-4","journal-title":"Int J Legal Med"},{"issue":"Suppl","key":"4046_CR20","doi-asserted-by":"publisher","first-page":"S151","DOI":"10.1016\/j.forsciint.2004.09.046","volume":"146","author":"D Di Martino","year":"2004","unstructured":"Di Martino D, Giuffr\u00e8 G, Staiti N, Simone A, Le Donne M, Saravo L (2004) Single sperm cell isolation by laser microdissection. Forensic Sci Int 146(Suppl):S151\u2013S153. https:\/\/doi.org\/10.1016\/j.forsciint.2004.09.046","journal-title":"Forensic Sci Int"},{"issue":"4","key":"4046_CR21","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1111\/1556-4029.13348","volume":"62","author":"S Costa","year":"2017","unstructured":"Costa S, Correia-de-S\u00e1 P, Porto MJ, Cain\u00e9 L (2017) The use of laser microdissection in forensic sexual assault casework: pros and cons compared to standard methods. J Forensic Sci 62(4):998\u20131006. https:\/\/doi.org\/10.1111\/1556-4029.13348","journal-title":"J Forensic Sci"},{"issue":"8","key":"4046_CR22","doi-asserted-by":"publisher","first-page":"e0308662","DOI":"10.1371\/journal.pone.0308662","volume":"19","author":"B Zhou","year":"2024","unstructured":"Zhou B, Huang C, Yi D (2024) Laser microdissection system based on structured light modulation dual cutting mode and negative pressure adsorption collection. PLoS ONE 19(8):e0308662. https:\/\/doi.org\/10.1371\/journal.pone.0308662","journal-title":"PLoS ONE"},{"issue":"5","key":"4046_CR23","doi-asserted-by":"publisher","first-page":"1704","DOI":"10.1111\/1556-4029.70131","volume":"70","author":"J Schulte","year":"2025","unstructured":"Schulte J, Egger S, Kron S, Scheurer E, Schulz I (2025) Evaluating novel and conventional cell-separation techniques for sexual assault investigations. J Forensic Sci 70(5):1704\u20131720. https:\/\/doi.org\/10.1111\/1556-4029.70131","journal-title":"J Forensic Sci"},{"issue":"6","key":"4046_CR24","doi-asserted-by":"publisher","first-page":"2209","DOI":"10.1007\/s00414-024-03285-1","volume":"138","author":"HR Dash","year":"2024","unstructured":"Dash HR (2024) Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases. Int J Legal Med 138(6):2209\u20132227. https:\/\/doi.org\/10.1007\/s00414-024-03285-1","journal-title":"Int J Legal Med"},{"key":"4046_CR25","doi-asserted-by":"publisher","first-page":"209","DOI":"10.4467\/12307483pfs.24.014.20914","volume":"139","author":"S Kumar","year":"2024","unstructured":"Kumar S, Baggi TR (2024) The role of flow cytometry in forensic investigation: a review. Probl Forensic Sci 139:209\u2013226. https:\/\/doi.org\/10.4467\/12307483pfs.24.014.20914","journal-title":"Probl Forensic Sci"},{"issue":"4","key":"4046_CR26","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/S0029-7844(99)00373-7","volume":"94","author":"WM Schoell","year":"1999","unstructured":"Schoell WM, Klintschar M, Mirhashemi R, Pertl B (1999) Separation of sperm and vaginal cells with flow cytometry for DNA typing after sexual assault. Obstet Gynecol 94(4):623\u2013627. https:\/\/doi.org\/10.1016\/S0029-7844(99)00373-7","journal-title":"Obstet Gynecol"},{"issue":"4","key":"4046_CR27","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1002\/(sici)1097-0320(19990801)36:4<319::aid-cyto6>3.3.co;2-c","volume":"36","author":"WM Schoell","year":"1999","unstructured":"Schoell WM, Klintschar M, Mirhashemi R, Strunk D, Giuliani A, Bogensberger G, Pertl B (1999) Separation of sperm and vaginal cells based on ploidy, MHC class I-, CD45-, and cytokeratin expression for enhancement of DNA typing after sexual assault. Cytometry 36(4):319\u2013323. https:\/\/doi.org\/10.1002\/(sici)1097-0320(19990801)36:4%3C319::aid-cyto6%3E3.3.co;2-c","journal-title":"Cytometry"},{"key":"4046_CR28","doi-asserted-by":"publisher","first-page":"36515","DOI":"10.1038\/srep36515","volume":"6","author":"Y Xu","year":"2016","unstructured":"Xu Y, Xie J, Chen R, Cao Y, Ping Y, Xu Q, Hu W, Wu D, Gu L, Zhou H, Chen X, Zhao Z, Zhong J, Li R (2016) Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification. Sci Rep 6:36515. https:\/\/doi.org\/10.1038\/srep36515","journal-title":"Sci Rep"},{"issue":"8Supplement1","key":"4046_CR29","doi-asserted-by":"publisher","first-page":"2109","DOI":"10.1158\/1538-7445.AM2025-2109","volume":"85","author":"HL Veloz","year":"2025","unstructured":"Veloz HL, Hill SM, Asantewaa G, Gallegos LL, Alexander NR (2025) Comparison of four non-enzymatic, mechanical dissociation methods for formalin-fixed tissue in flow cytometric analysis and single-cell workflows. Cancer Res 85(8Supplement1):2109. https:\/\/doi.org\/10.1158\/1538-7445.AM2025-2109","journal-title":"Cancer Res"},{"key":"4046_CR30","doi-asserted-by":"publisher","first-page":"112274","DOI":"10.1016\/j.forsciint.2024.112274","volume":"365","author":"D Ross","year":"2024","unstructured":"Ross D, Taylor D, van Oorschot RAH, Best G, Goray M (2024) Classification of epidermal, buccal, penile and vaginal epithelial cells using morphological characteristics measured by imaging flow cytometry. Forensic Sci Int 365:112274. https:\/\/doi.org\/10.1016\/j.forsciint.2024.112274","journal-title":"Forensic Sci Int"},{"issue":"8","key":"4046_CR31","doi-asserted-by":"publisher","first-page":"16897","DOI":"10.3390\/ijms160816897","volume":"16","author":"A Gross","year":"2015","unstructured":"Gross A, Schoendube J, Zimmermann S, Steeb M, Zengerle R, Koltay P (2015) Technologies for Single-Cell Isolation. Int J Mol Sci 16(8):16897\u201316919. https:\/\/doi.org\/10.3390\/ijms160816897","journal-title":"Int J Mol Sci"},{"issue":"2","key":"4046_CR32","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1002\/cyto.a.23690","volume":"95","author":"A Reichard","year":"2019","unstructured":"Reichard A, Asosingh K (2019) Best practices for preparing a single cell suspension from solid tissues for flow cytometry. Cytometry A 95(2):219\u2013226. https:\/\/doi.org\/10.1002\/cyto.a.23690","journal-title":"Cytometry A"},{"issue":"1","key":"4046_CR33","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1186\/s13059-022-02824-6","volume":"23","author":"J Park","year":"2022","unstructured":"Park J, Kim J, Lewy T, Rice CM, Elemento O, Rendeiro AF, Mason CE (2022) Spatial omics technologies at multimodal and single cell\/subcellular level. Genome Biol 23(1):256. https:\/\/doi.org\/10.1186\/s13059-022-02824-6","journal-title":"Genome Biol"},{"issue":"5","key":"4046_CR34","doi-asserted-by":"publisher","first-page":"140","DOI":"10.19650\/j.cnki.cjsi.J2006185","volume":"41","author":"CH Huang","year":"2020","unstructured":"Huang CH, Yi DR, Jin FJ, Ye YQ (2020) Progress on single cell isolation methods and instruments. Chin J Sci Instrument 41(5):140\u2013153. https:\/\/doi.org\/10.19650\/j.cnki.cjsi.J2006185","journal-title":"Chin J Sci Instrument"},{"issue":"6","key":"4046_CR35","doi-asserted-by":"publisher","first-page":"934","DOI":"10.19756\/j.issn.0253-3820.231008","volume":"51","author":"T Pan","year":"2023","unstructured":"Pan T, Wu YY, Guo GS, Wang XY (2023) Advances in microfluidic chip structures based on hydrodynamics of efficient single-cell capture. Chin J Anal Chem 51(6):934\u2013944. https:\/\/doi.org\/10.19756\/j.issn.0253-3820.231008","journal-title":"Chin J Anal Chem"},{"issue":"4","key":"4046_CR36","doi-asserted-by":"publisher","first-page":"044127","DOI":"10.1063\/1.4928453","volume":"9","author":"W Liu","year":"2015","unstructured":"Liu W, Chen W, Liu R, Ou Y, Liu H, Xie L, Lu Y, Li C, Li B, Cheng J (2015) Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis. Biomicrofluidics 9(4):044127. https:\/\/doi.org\/10.1063\/1.4928453","journal-title":"Biomicrofluidics"},{"issue":"3","key":"4046_CR37","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1039\/d3lc00632h","volume":"24","author":"TY Wan","year":"2024","unstructured":"Wan TY, Hwa HL, Lee TT, Lu YW (2024) High efficiency sperm enrichment from forensic mock samples in bubble-based acoustic filtration devices for short tandem repeat (STR) analysis. Lab Chip 24(3):434\u2013445. https:\/\/doi.org\/10.1039\/d3lc00632h","journal-title":"Lab Chip"},{"issue":"3","key":"4046_CR38","doi-asserted-by":"publisher","first-page":"339","DOI":"10.13618\/j.issn.1001-5728.2024.03.017","volume":"39","author":"KS Sun","year":"2024","unstructured":"Sun KS, Gu HY, Song F, Dong YC, Luo HB (2024) Progress in single cell isolation techniques in forensic science. Chin J Forensic Med 39(3):339\u2013348. https:\/\/doi.org\/10.13618\/j.issn.1001-5728.2024.03.017","journal-title":"Chin J Forensic Med"},{"issue":"4","key":"4046_CR39","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1111\/1556-4029.15514","volume":"69","author":"J Hogg","year":"2024","unstructured":"Hogg J, Vandepoele ACW, Zaccheo N, Schulte J, Schulz I, Dubois J, Frank M, Marciano MA (2024) Targeted recovery of male cells in a male and female same-cell mixture. J Forensic Sci 69(4):1183\u20131197. https:\/\/doi.org\/10.1111\/1556-4029.15514","journal-title":"J Forensic Sci"},{"key":"4046_CR40","doi-asserted-by":"publisher","first-page":"103026","DOI":"10.1016\/j.fsigen.2024.103026","volume":"70","author":"J Schulte","year":"2024","unstructured":"Schulte J, Caliebe A, Marciano M, Neuschwander P, Seiberle I, Scheurer E, Schulz I (2024) DEPArray\u2122 single-cell technology: A validation study for forensic applications. Forensic Sci Int Genet 70:103026. https:\/\/doi.org\/10.1016\/j.fsigen.2024.103026","journal-title":"Forensic Sci Int Genet"},{"key":"4046_CR41","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.fsigen.2018.03.001","volume":"34","author":"VR Williamson","year":"2018","unstructured":"Williamson VR, Laris TM, Romano R, Marciano MA (2018) Enhanced DNA mixture deconvolution of sexual offense samples using the DEPArray\u2122 system. Forensic Sci Int Genet 34:265\u2013276. https:\/\/doi.org\/10.1016\/j.fsigen.2018.03.001","journal-title":"Forensic Sci Int Genet"},{"issue":"6","key":"4046_CR42","doi-asserted-by":"publisher","first-page":"1358","DOI":"10.1038\/bjc.2013.92","volume":"108","author":"DJ Peeters","year":"2013","unstructured":"Peeters DJ, De Laere B, Van den Eynden GG, Van Laere SJ, Roth\u00e9 F, Ignatiadis M, Sieuwerts AM, Lambrechts D, Rutten A, van Dam PA, Pauwels P, Peeters M, Vermeulen PB, Dirix LY (2013) Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting. Br J Cancer 108(6):1358\u20131367. https:\/\/doi.org\/10.1038\/bjc.2013.92","journal-title":"Br J Cancer"},{"issue":"3","key":"4046_CR43","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1039\/D5LC00945F","volume":"26","author":"TN Rathnaweera","year":"2026","unstructured":"Rathnaweera TN, Rajkumar D, Anand RK (2026) Integrated DEP presorting and wireless electrode array for high-throughput selective single-cell isolation. Lab Chip 26(3):681\u2013694. https:\/\/doi.org\/10.1039\/D5LC00945F","journal-title":"Lab Chip"},{"issue":"6651","key":"4046_CR44","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1038\/39225","volume":"389","author":"I Findlay","year":"1997","unstructured":"Findlay I, Taylor A, Quirke P, Frazier R, Urquhart A (1997) DNA fingerprinting from single cells. Nature 389(6651):555\u2013556. https:\/\/doi.org\/10.1038\/39225","journal-title":"Nature"},{"key":"4046_CR45","doi-asserted-by":"publisher","first-page":"102211","DOI":"10.1016\/j.fsigen.2019.102211","volume":"45","author":"M Chen","year":"2020","unstructured":"Chen M, Zhang J, Zhao J, Chen T, Liu Z, Cheng F, Fan Q, Yan J (2020) Comparison of CE- and MPS-based analyses of forensic markers in a single cell after whole genome amplification. Forensic Sci Int Genet 45:102211. https:\/\/doi.org\/10.1016\/j.fsigen.2019.102211","journal-title":"Forensic Sci Int Genet"},{"issue":"1","key":"4046_CR46","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1038\/s41598-018-19509-5","volume":"8","author":"L Deleye","year":"2018","unstructured":"Deleye L, Vander Plaetsen AS, Weymaere J, Deforce D, Van Nieuwerburgh F (2018) Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells. Sci Rep 8(1):1255. https:\/\/doi.org\/10.1038\/s41598-018-19509-5","journal-title":"Sci Rep"},{"issue":"6","key":"4046_CR47","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1002\/elps.201600385","volume":"38","author":"KR Duffy","year":"2017","unstructured":"Duffy KR, Gurram N, Peters KC, Wellner G, Grgicak CM (2017) Exploring STR signal in the single- and multicopy number regimes: Deductions from an in silico model of the entire DNA laboratory process. Electrophoresis 38(6):855\u2013868. https:\/\/doi.org\/10.1002\/elps.201600385","journal-title":"Electrophoresis"},{"issue":"1","key":"4046_CR48","doi-asserted-by":"publisher","first-page":"35736","DOI":"10.1038\/s41598-025-19847-1","volume":"15","author":"Q Ji","year":"2025","unstructured":"Ji Q, Li Z, Wang Z, Yu H, Yang F, Xie J (2025) Improving STR typing for low template DNA by abasic-site-mediated semi-linear preamplification. Sci Rep 15(1):35736. https:\/\/doi.org\/10.1038\/s41598-025-19847-1","journal-title":"Sci Rep"},{"issue":"3","key":"4046_CR49","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1007\/s00414-021-02503-4","volume":"135","author":"N Sheth","year":"2021","unstructured":"Sheth N, Swaminathan H, Gonzalez AJ, Duffy KR, Grgicak CM (2021) Towards developing forensically relevant single-cell pipelines by incorporating direct-to-PCR extraction: compatibility, signal quality, and allele detection. Int J Legal Med 135(3):727\u2013738. https:\/\/doi.org\/10.1007\/s00414-021-02503-4","journal-title":"Int J Legal Med"},{"key":"4046_CR50","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1007\/s00194-023-00673-6","volume":"34","author":"K Anslinger","year":"2024","unstructured":"Anslinger K, Bayer B, Schick S et al (2024) Using cluster analysis for grouping partial autosomal haplotypes derived from single sperm STR profiling. Rechtsmedizin 34:108\u2013114. https:\/\/doi.org\/10.1007\/s00194-023-00673-6","journal-title":"Rechtsmedizin"},{"key":"4046_CR51","doi-asserted-by":"publisher","first-page":"102852","DOI":"10.1016\/j.fsigen.2023.102852","volume":"64","author":"KR Duffy","year":"2023","unstructured":"Duffy KR, Lun DS, Mulcahy MM, O\u2019Donnell L, Sheth N, Grgicak CM (2023) Evidentiary evaluation of single cells renders highly informative forensic comparisons across multifarious admixtures. Forensic Sci Int Genet 64:102852. https:\/\/doi.org\/10.1016\/j.fsigen.2023.102852","journal-title":"Forensic Sci Int Genet"},{"key":"4046_CR52","doi-asserted-by":"publisher","first-page":"103000","DOI":"10.1016\/j.fsigen.2023.103000","volume":"69","author":"CM Grgicak","year":"2024","unstructured":"Grgicak CM, Bhembe Q, Slooten K, Sheth NC, Duffy KR, Lun DS (2024) Single-cell investigative genetics: Single-cell data produces genotype distributions concentrated at the true genotype across all mixture complexities. Forensic Sci Int Genet 69:103000. https:\/\/doi.org\/10.1016\/j.fsigen.2023.103000","journal-title":"Forensic Sci Int Genet"},{"issue":"1","key":"4046_CR53","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.scijus.2020.10.005","volume":"61","author":"K Huffman","year":"2021","unstructured":"Huffman K, Hanson E, Ballantyne J (2021) Recovery of single source DNA profiles from mixtures by direct single cell subsampling and simplified micromanipulation. Sci Justice 61(1):13\u201325. https:\/\/doi.org\/10.1016\/j.scijus.2020.10.005","journal-title":"Sci Justice"},{"key":"4046_CR54","doi-asserted-by":"publisher","first-page":"103211","DOI":"10.1016\/j.fsigen.2024.103211","volume":"76","author":"ACW Vandepoele","year":"2025","unstructured":"Vandepoele ACW, Novotna N, Myers D, Marciano MA (2025) Characterizing stutter in single cells and the impact on multi-cell analysis. Forensic Sci Int Genet 76:103211. https:\/\/doi.org\/10.1016\/j.fsigen.2024.103211","journal-title":"Forensic Sci Int Genet"},{"issue":"5","key":"4046_CR55","doi-asserted-by":"publisher","first-page":"1687","DOI":"10.1111\/1556-4029.70103","volume":"70","author":"M Peters","year":"2025","unstructured":"Peters M, Huffman K, Ballantyne J, Hanson E (2025) Probabilistic genotyping replicate analysis of FaSTR clustered single sperm aSTR haplotypes reconstitutes probative diploid DNA genotypes from complex semen mixtures. J Forensic Sci 70(5):1687\u20131703. https:\/\/doi.org\/10.1111\/1556-4029.70103","journal-title":"J Forensic Sci"},{"key":"4046_CR56","doi-asserted-by":"publisher","first-page":"674","DOI":"10.3390\/genes14030674","volume":"14","author":"K Huffman","year":"2023","unstructured":"Huffman K, Ballantyne J (2023) Validation of probabilistic genotyping software for single cell STR analysis. Genes 14:674. https:\/\/doi.org\/10.3390\/genes14030674","journal-title":"Genes"},{"key":"4046_CR57","doi-asserted-by":"publisher","first-page":"102908","DOI":"10.1016\/j.fsigen.2023.102908","volume":"66","author":"K Huffman","year":"2023","unstructured":"Huffman K, Kruijver M, Ballantyne J, Taylor D (2023) Carrying out common DNA donor analysis using DBLR\u2122 on two or five-cell mini-mixture subsamples for improved discrimination power in complex DNA mixtures. Forensic Sci Int Genet 66:102908. https:\/\/doi.org\/10.1016\/j.fsigen.2023.102908","journal-title":"Forensic Sci Int Genet"},{"key":"4046_CR58","doi-asserted-by":"publisher","first-page":"103220","DOI":"10.1016\/j.fsigen.2024.103220","volume":"76","author":"CM Grgicak","year":"2025","unstructured":"Grgicak CM, Slooten K, Cowell RG, Bhembe Q, Lun DS (2025) The (in)dependence of single-cell data inferences on model constructs. Forensic Sci Int Genet 76:103220. https:\/\/doi.org\/10.1016\/j.fsigen.2024.103220","journal-title":"Forensic Sci Int Genet"},{"issue":"1","key":"4046_CR59","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1038\/s42003-023-04557-z","volume":"6","author":"L Kulhankova","year":"2023","unstructured":"Kulhankova L, Montiel Gonz\u00e1lez D, Bindels E, Kling D, Kayser M, Mulugeta E (2023) Single-cell transcriptome sequencing allows genetic separation, characterization and identification of individuals in multi-person biological mixtures. Commun Biol 6(1):201. https:\/\/doi.org\/10.1038\/s42003-023-04557-z","journal-title":"Commun Biol"},{"key":"4046_CR60","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.fsigen.2014.08.012","volume":"13","author":"JP Han","year":"2014","unstructured":"Han JP, Yang F, Xu C, Wei YL, Zhao XC, Hu L, Ye J, Li CX (2014) A new strategy for sperm isolation and STR typing from multi-donor sperm mixtures. Forensic Sci Int Genet 13:239\u2013246. https:\/\/doi.org\/10.1016\/j.fsigen.2014.08.012","journal-title":"Forensic Sci Int Genet"},{"issue":"5","key":"4046_CR61","doi-asserted-by":"publisher","first-page":"537","DOI":"10.3325\/cmj.2014.55.537","volume":"55","author":"L Feng","year":"2014","unstructured":"Feng L, Xu C, Zeng X, Zhang H, Yang F, Li W, Tu Z, Li C, Hu L (2014) Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case. Croat Med J 55(5):537\u2013541. https:\/\/doi.org\/10.3325\/cmj.2014.55.537","journal-title":"Croat Med J"},{"key":"4046_CR62","doi-asserted-by":"publisher","first-page":"7269237","DOI":"10.1155\/2021\/7269237","volume":"2021","author":"ML Sun","year":"2021","unstructured":"Sun ML, Zheng JL, Wang BJ, Yao J (2021) Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains. Biomed Res Int 2021:7269237. https:\/\/doi.org\/10.1155\/2021\/7269237","journal-title":"Biomed Res Int"},{"issue":"19\u201320","key":"4046_CR63","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1002\/elps.202300021","volume":"44","author":"J Yao","year":"2023","unstructured":"Yao J, Adnan A, Wang HB (2023) Separation mixed semen of two individuals using magnetic beads coupled ABH blood group antibody. Electrophoresis 44(19\u201320):1539\u20131547. https:\/\/doi.org\/10.1002\/elps.202300021","journal-title":"Electrophoresis"},{"key":"4046_CR64","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.fsigen.2017.04.023","volume":"29","author":"F Fontana","year":"2017","unstructured":"Fontana F, Rapone C, Bregola G, Aversa R, de Meo A, Signorini G, Sergio M, Ferrarini A, Lanzellotto R, Medoro G, Giorgini G, Manaresi N, Berti A (2017) Isolation and genetic analysis of pure cells from forensic biological mixtures: The precision of a digital approach. Forensic Sci Int Genet 29:225\u2013241. https:\/\/doi.org\/10.1016\/j.fsigen.2017.04.023","journal-title":"Forensic Sci Int Genet"},{"issue":"3","key":"4046_CR65","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.1111\/1556-4029.70027","volume":"70","author":"DNO Bonsu","year":"2025","unstructured":"Bonsu DNO, Mitchell N, Jeanes C, Henry J (2025) Investigation of a Y-chromosome screening approach for sexual assault evidence using the QIAGEN Investigator Casework GO! kit. J Forensic Sci 70(3):1133\u20131148. https:\/\/doi.org\/10.1111\/1556-4029.70027","journal-title":"J Forensic Sci"},{"key":"4046_CR66","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.fsigen.2018.11.019","volume":"39","author":"J Burrill","year":"2019","unstructured":"Burrill J, Daniel B, Frascione N (2019) A review of trace Touch DNA deposits: Variability factors and an exploration of cellular composition. Forensic Sci Int Genet 39:8\u201318. https:\/\/doi.org\/10.1016\/j.fsigen.2018.11.019","journal-title":"Forensic Sci Int Genet"},{"issue":"Suppl 1","key":"4046_CR67","doi-asserted-by":"publisher","first-page":"S576","DOI":"10.1016\/j.legalmed.2009.02.004","volume":"11","author":"T Tokutomi","year":"2009","unstructured":"Tokutomi T, Takada Y, Kanetake J, Mukaida M (2009) Identification using DNA from skin contact: case reports. Leg Med (Tokyo) 11(Suppl 1):S576\u2013S577. https:\/\/doi.org\/10.1016\/j.legalmed.2009.02.004","journal-title":"Leg Med (Tokyo)"},{"issue":"24","key":"4046_CR68","doi-asserted-by":"publisher","first-page":"15541","DOI":"10.3390\/ijms232415541","volume":"23","author":"P Tozzo","year":"2022","unstructured":"Tozzo P, Mazzobel E, Marcante B, Delicati A, Caenazzo L (2022) Touch DNA Sampling Methods: Efficacy Evaluation and Systematic Review. Int J Mol Sci 23(24):15541. https:\/\/doi.org\/10.3390\/ijms232415541","journal-title":"Int J Mol Sci"},{"key":"4046_CR69","doi-asserted-by":"publisher","first-page":"9","DOI":"10.16467\/j.1008-3650.2013.03.001","volume":"3","author":"GF Zhang","year":"2013","unstructured":"Zhang GF, Chen S, Tu Z, Ge YY (2013) Discussion on the factors affecting success rate of touch DNA analysis. Forensic Sci Technol 3:9\u201313. https:\/\/doi.org\/10.16467\/j.1008-3650.2013.03.001","journal-title":"Forensic Sci Technol"},{"key":"4046_CR70","doi-asserted-by":"publisher","first-page":"111102","DOI":"10.1016\/j.forsciint.2021.111102","volume":"330","author":"J Burrill","year":"2022","unstructured":"Burrill J, Daniel B, Frascione N (2022) Technical note: lysis and purification methods for increased recovery of degraded DNA from touch deposit swabs. Forensic Sci Int 330:111102. https:\/\/doi.org\/10.1016\/j.forsciint.2021.111102","journal-title":"Forensic Sci Int"},{"issue":"1","key":"4046_CR71","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1111\/1556-4029.15150","volume":"68","author":"K Huffman","year":"2023","unstructured":"Huffman K, Hanson E, Ballantyne J (2023) Y-STR mixture deconvolution by single-cell analysis. J Forensic Sci 68(1):275\u2013288. https:\/\/doi.org\/10.1111\/1556-4029.15150","journal-title":"J Forensic Sci"},{"issue":"3","key":"4046_CR72","doi-asserted-by":"publisher","first-page":"293","DOI":"10.3325\/cmj.2011.52.293","volume":"52","author":"CX Li","year":"2011","unstructured":"Li CX, Wang GQ, Li WS, Huang JP, Ji AQ, Hu L (2011) New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks. Croat Med J 52(3):293\u2013298. https:\/\/doi.org\/10.3325\/cmj.2011.52.293","journal-title":"Croat Med J"},{"issue":"1","key":"4046_CR73","doi-asserted-by":"publisher","first-page":"28","DOI":"10.2334\/josnusd.18-0408","volume":"62","author":"S Maruyama","year":"2020","unstructured":"Maruyama S, Tsutsumi H, Izawa H, Komuro T (2020) Short tandem repeat typing of cells transferred via micromanipulation with whole-genome amplification. J Oral Sci 62(1):28\u201331. https:\/\/doi.org\/10.2334\/josnusd.18-0408","journal-title":"J Oral Sci"},{"issue":"3","key":"4046_CR74","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/j.fsigen.2009.09.007","volume":"4","author":"R Alaeddini","year":"2010","unstructured":"Alaeddini R, Walsh SJ, Abbas A (2010) Forensic implications of genetic analyses from degraded DNA. Forensic Sci Int Genet 4(3):148\u2013157. https:\/\/doi.org\/10.1016\/j.fsigen.2009.09.007","journal-title":"Forensic Sci Int Genet"},{"key":"4046_CR75","doi-asserted-by":"publisher","unstructured":"Zhang YX, Zhao XT, Ai K, Li XP, Zhang L (2025) Current status and advances in DNA extraction methods for trace degraded forensic samples. Life Sci Res 1\u201310. https:\/\/doi.org\/10.16605\/j.cnki.1007-7847.2025.09.0169","DOI":"10.16605\/j.cnki.1007-7847.2025.09.0169"},{"key":"4046_CR76","doi-asserted-by":"publisher","first-page":"103342","DOI":"10.1016\/j.fsigen.2025.103342","volume":"81","author":"S Nakao","year":"2025","unstructured":"Nakao S, Kitagawa M, Suzuki K, Sato T (2025) Effective use of the degradation index from human DNA quantification kits to improve STR and Y-STR profiling. Forensic Sci Int Genet 81:103342. https:\/\/doi.org\/10.1016\/j.fsigen.2025.103342","journal-title":"Forensic Sci Int Genet"},{"key":"4046_CR77","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.fsigen.2014.08.003","volume":"13","author":"T Lepez","year":"2014","unstructured":"Lepez T, Vandewoestyne M, Van Hoofstat D, Deforce D (2014) Fast nuclear staining of head hair roots as a screening method for successful STR analysis in forensics. Forensic Sci Int Genet 13:191\u2013194. https:\/\/doi.org\/10.1016\/j.fsigen.2014.08.003","journal-title":"Forensic Sci Int Genet"},{"issue":"4","key":"4046_CR78","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1089\/forensic.2023.0017","volume":"3","author":"LM Otterstatter","year":"2023","unstructured":"Otterstatter LM, Espinoza Quiroga IA, Betts SM et al (2023) Revisiting the hair root visible nuclei count threshold for nuclear DNA typing. Forensic Genomics 3(4):138\u2013149. https:\/\/doi.org\/10.1089\/forensic.2023.0017","journal-title":"Forensic Genomics"},{"key":"4046_CR79","doi-asserted-by":"publisher","first-page":"102602","DOI":"10.1016\/j.fsigen.2021.102602","volume":"56","author":"R Golomingi","year":"2022","unstructured":"Golomingi R, Haas C, Dobay A, Kottner S, Ebert LC (2022) Sperm hunting on optical microscope slides for forensic analysis with deep convolutional networks\u2014A feasibility study. Forensic Sci Int Genet 56:102602. https:\/\/doi.org\/10.1016\/j.fsigen.2021.102602","journal-title":"Forensic Sci Int Genet"},{"issue":"3","key":"4046_CR80","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.3390\/s23031075","volume":"23","author":"B Bruijns","year":"2023","unstructured":"Bruijns B, Knotter J, Tiggelaar R (2023) A Systematic Review on Commercially Available Integrated Systems for Forensic DNA Analysis. Sens (Basel) 23(3):1075. https:\/\/doi.org\/10.3390\/s23031075","journal-title":"Sens (Basel)"},{"key":"4046_CR81","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.fsigen.2014.12.004","volume":"16","author":"S Jovanovich","year":"2015","unstructured":"Jovanovich S, Bogdan G, Belcinski R et al (2015) Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples. Forensic Sci Int Genet 16:181\u2013194. https:\/\/doi.org\/10.1016\/j.fsigen.2014.12.004","journal-title":"Forensic Sci Int Genet"},{"issue":"4","key":"4046_CR82","doi-asserted-by":"publisher","first-page":"1336","DOI":"10.1111\/1556-4029.15044","volume":"67","author":"M Harrel","year":"2022","unstructured":"Harrel M, Holmes AS (2022) Review of direct PCR and Rapid DNA approaches to streamline sexual assault kit testing. J Forensic Sci 67(4):1336\u20131347. https:\/\/doi.org\/10.1111\/1556-4029.15044","journal-title":"J Forensic Sci"},{"issue":"1","key":"4046_CR83","doi-asserted-by":"publisher","first-page":"101370","DOI":"10.1016\/j.scijus.2025.101370","volume":"66","author":"DC Topping","year":"2026","unstructured":"Topping DC, Tran T, Hymus CM, Mountford NS, Liew YC, Egan SE, Cooper PL (2026) Automated microscopy in forensic biology: Validating the use of deep neural networks for the detection of human spermatozoa in Christmas Tree stained slides. Sci Justice 66(1):101370. https:\/\/doi.org\/10.1016\/j.scijus.2025.101370","journal-title":"Sci Justice"},{"key":"4046_CR84","doi-asserted-by":"publisher","first-page":"103430","DOI":"10.1016\/j.fsigen.2026.103430","volume":"82","author":"UN Koh","year":"2026","unstructured":"Koh UN, Sunkara V, Ahn B, Jung MK, Jang M, Ryu H, Kim H, Ahn J, Lee K, Lim SK, Cho YK, Lee B (2026) Forensic Lab-on-a-Disc for enhanced sperm cell purification in differential extraction workflows. Forensic Sci Int Genet 82:103430. https:\/\/doi.org\/10.1016\/j.fsigen.2026.103430","journal-title":"Forensic Sci Int Genet"},{"issue":"1","key":"4046_CR85","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1021\/ac403137h","volume":"86","author":"T Geng","year":"2014","unstructured":"Geng T, Novak R, Mathies RA (2014) Single-cell forensic short tandem repeat typing within microfluidic droplets. Anal Chem 86(1):703\u2013712. https:\/\/doi.org\/10.1021\/ac403137h","journal-title":"Anal Chem"},{"issue":"1","key":"4046_CR86","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.fsigen.2011.02.007","volume":"6","author":"M Meredith","year":"2012","unstructured":"Meredith M, Bright JA, Cockerton S, Vintiner S (2012) Development of a one-tube extraction and amplification method for DNA analysis of sperm and epithelial cells recovered from forensic samples by laser microdissection. Forensic Sci Int Genet 6(1):91\u201396. https:\/\/doi.org\/10.1016\/j.fsigen.2011.02.007","journal-title":"Forensic Sci Int Genet"},{"issue":"49","key":"4046_CR87","doi-asserted-by":"publisher","first-page":"e07944","DOI":"10.1002\/smll.202507944","volume":"21","author":"Y Li","year":"2025","unstructured":"Li Y, Zhuang B, Han J, Feng J, Zhao L, Liu Z, Jiang L, Yao Y, Xing W, Li C (2025) A Versatile Sample-in-Answer-Out Microfluidic System for Combined Detection of Multi-Functional Forensic Human Genetic Markers. Small 21(49):e07944. https:\/\/doi.org\/10.1002\/smll.202507944","journal-title":"Small"},{"issue":"9\u201310","key":"4046_CR88","doi-asserted-by":"publisher","first-page":"814","DOI":"10.1002\/elps.202300187","volume":"45","author":"J Han","year":"2024","unstructured":"Han J, Zhuang B, Zou L, Wang D, Jiang L, Wei YL, Zhao L, Zhao L, Li C (2024) A developmental validation of the Quick TargSeq 1.0 integrated system for automated DNA genotyping in forensic science for reference samples. Electrophoresis 45(9\u201310):814\u2013828. https:\/\/doi.org\/10.1002\/elps.202300187","journal-title":"Electrophoresis"},{"issue":"20","key":"4046_CR89","doi-asserted-by":"publisher","first-page":"1534","DOI":"10.1002\/elps.70029","volume":"46","author":"Y Li","year":"2025","unstructured":"Li Y, Jiang L, Liu Q, Chen B, Zhuang B, Zhao L, Han J, Li C (2025) Integrated Microfluidic System for Rapid 89-Plex Y-SNP Profiling: Development and Forensic Validation. Electrophoresis 46(20):1534\u20131547. https:\/\/doi.org\/10.1002\/elps.70029","journal-title":"Electrophoresis"},{"key":"4046_CR90","doi-asserted-by":"publisher","first-page":"40531","DOI":"10.1038\/s41598-025-24283-2","volume":"15","author":"S \u0160kevin","year":"2025","unstructured":"\u0160kevin S, De Keyzer L, De Waele L, Tytgat O, Van Hoofstat D, Deforce D, Van Nieuwerburgh F (2025) Recombinase polymerase amplification of forensic short tandem repeat loci. Sci Rep 15:40531. https:\/\/doi.org\/10.1038\/s41598-025-24283-2","journal-title":"Sci Rep"},{"issue":"2","key":"4046_CR91","doi-asserted-by":"publisher","first-page":"106","DOI":"10.3390\/bios16020106","volume":"16","author":"L De Keyzer","year":"2026","unstructured":"De Keyzer L, \u0160kevin S, Deserranno K, Deforce D, Van Nieuwerburgh F (2026) Rapid Forensic DNA Profiling via Real-Time Recombinase Polymerase Amplification of InDel Markers. Biosensors 16(2):106. https:\/\/doi.org\/10.3390\/bios16020106","journal-title":"Biosensors"},{"issue":"11","key":"4046_CR92","doi-asserted-by":"publisher","first-page":"1649","DOI":"10.3390\/genes12111649","volume":"12","author":"J Ge","year":"2021","unstructured":"Ge J, King JL, Smuts A, Budowle B (2021) Precision DNA Mixture Interpretation with Single-Cell Profiling. Genes 12(11):1649. https:\/\/doi.org\/10.3390\/genes12111649","journal-title":"Genes"},{"issue":"2","key":"4046_CR93","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.scijus.2022.01.003","volume":"62","author":"K Huffman","year":"2022","unstructured":"Huffman K, Hanson E, Ballantyne J (2022) Probabilistic genotyping of single cell replicates from complex DNA mixtures recovers higher contributor LRs than standard analysis. Sci Justice 62(2):156\u2013163. https:\/\/doi.org\/10.1016\/j.scijus.2022.01.003","journal-title":"Sci Justice"},{"issue":"10","key":"4046_CR94","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.3390\/genes12101559","volume":"12","author":"P Gill","year":"2021","unstructured":"Gill P, Benschop C, Buckleton J, Bleka \u00d8, Taylor D (2021) A Review of Probabilistic Genotyping Systems: EuroForMix, DNAStatistX and STRmix\u2122. Genes 12(10):1559. https:\/\/doi.org\/10.3390\/genes12101559","journal-title":"Genes"},{"key":"4046_CR95","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.fsigen.2018.11.009","volume":"38","author":"MD Coble","year":"2019","unstructured":"Coble MD, Bright J-A (2019) Probabilistic genotyping software: An overview. Forensic Sci Int Genet 38:219\u2013224. https:\/\/doi.org\/10.1016\/j.fsigen.2018.11.009","journal-title":"Forensic Sci Int Genet"},{"issue":"5","key":"4046_CR96","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1016\/j.fsigen.2013.05.011","volume":"7","author":"D Taylor","year":"2013","unstructured":"Taylor D, Bright JA, Buckleton J (2013) The interpretation of single source and mixed DNA profiles. Forensic Sci Int Genet 7(5):516\u2013528. https:\/\/doi.org\/10.1016\/j.fsigen.2013.05.011","journal-title":"Forensic Sci Int Genet"},{"issue":"9","key":"4046_CR97","doi-asserted-by":"publisher","first-page":"1658","DOI":"10.3390\/genes13091658","volume":"13","author":"K Huffman","year":"2022","unstructured":"Huffman K, Ballantyne J (2022) Probabilistic Genotyping of Single Cell Replicates from Mixtures Involving First-Degree Relatives Prevents the False Inclusions of Non-Donor Relatives. Genes 13(9):1658. https:\/\/doi.org\/10.3390\/genes13091658","journal-title":"Genes"},{"issue":"23","key":"4046_CR98","doi-asserted-by":"publisher","first-page":"6100","DOI":"10.1039\/D5LC00216H","volume":"25","author":"J Cheng","year":"2025","unstructured":"Cheng J, Anne R, Chen YC (2025) Transforming microfluidics for single-cell analysis with robotics and artificial intelligence. Lab Chip 25(23):6100\u20136125. https:\/\/doi.org\/10.1039\/D5LC00216H","journal-title":"Lab Chip"},{"issue":"5","key":"4046_CR99","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1007\/s00414-025-03530-1","volume":"139","author":"HR Dash","year":"2025","unstructured":"Dash HR, Al-Snan NR (2025) DNA forensics at forty: the way forward. Int J Legal Med 139(5):2023\u20132046. https:\/\/doi.org\/10.1007\/s00414-025-03530-1","journal-title":"Int J Legal Med"},{"issue":"2","key":"4046_CR100","doi-asserted-by":"publisher","first-page":"121467","DOI":"10.4331\/wjbc.v17.i2.121467","volume":"17","author":"K Evangelou","year":"2026","unstructured":"Evangelou K, Angeli P, Polydorou A, Petropoulou T (2026) Single-cell deoxyribonucleic acid typing for forensic mixtures and trace evidence: Opportunities, validation requirements, and reporting limits. World J Biol Chem 17(2):121467. https:\/\/doi.org\/10.4331\/wjbc.v17.i2.121467","journal-title":"World J Biol Chem"},{"key":"4046_CR101","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.fsigen.2018.07.024","volume":"37","author":"JM Butler","year":"2018","unstructured":"Butler JM, Kline MC, Coble MD (2018) NIST interlaboratory studies involving DNA mixtures (MIX05 and MIX13): Variation observed and lessons learned. Forensic Sci Int Genet 37:81\u201394. https:\/\/doi.org\/10.1016\/j.fsigen.2018.07.024","journal-title":"Forensic Sci Int Genet"},{"key":"4046_CR102","doi-asserted-by":"publisher","first-page":"103046","DOI":"10.1016\/j.fsigen.2024.103046","volume":"71","author":"S Boodoosingh","year":"2024","unstructured":"Boodoosingh S, Kelly H, Curran JM, Kalafut T (2024) An inter-laboratory comparison of probabilistic genotyping parameters and evaluation of performance on DNA mixtures from different laboratories. Forensic Sci Int Genet 71:103046. https:\/\/doi.org\/10.1016\/j.fsigen.2024.103046","journal-title":"Forensic Sci Int Genet"},{"key":"4046_CR103","doi-asserted-by":"publisher","unstructured":"Alfonse LE, Garrett AD, Lun DS, Duffy KR, Grgicak CM (2018) A large-scale dataset of single and mixed-source short tandem repeat profiles to inform human identification strategies: PROVEDIt. Forensic Sci Int Genet 32:62-70. https:\/\/doi.org\/10.1016\/j.fsigen.2017.10.006","DOI":"10.1016\/j.fsigen.2017.10.006"}],"container-title":["International Journal of Legal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00414-026-04046-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00414-026-04046-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00414-026-04046-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T18:03:04Z","timestamp":1792432984000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00414-026-04046-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,19]]},"references-count":103,"alternative-id":["4046"],"URL":"https:\/\/doi.org\/10.1007\/s00414-026-04046-y","relation":{},"ISSN":["0937-9827","1437-1596"],"issn-type":[{"value":"0937-9827","type":"print"},{"value":"1437-1596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,19]]},"assertion":[{"value":"18 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","label":"Clinical trial number","group":{"name":"EthicsHeading","label":"Declarations"}}]}}