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Serological parameters (ELISA or CLIA analysis) and clinical presentation of 28 patients with definite LNB were compared to those of 36 patients with neurological symptoms mimicking LNB (mimics). Analysis was performed using receiver operating characteristic (ROC) and binary logistic regression.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Elevated IgG-titers had a high sensitivity for neuroborreliosis in both centers (0.95 and 1.0). The optimal cutoff-values were set to 26.35 in center A (ELISA), and 64.0 in center B (CLIA). Diagnostic specificity was 0.41 and 0.89 in this constellation. Elevated IgM-titers showed a high diagnostic specificity for a cutoff at 68.10 (A) and 47.95 (B) (0.93 and 0.89). Sensitivity was 0.45 and 0.5. Overall diagnostic accuracy was low in both centers (A: IgG AUC\u2009=\u20090.665, IgM AUC\u2009=\u20090.629; B: IgG AUC\u2009=\u20090.917, IgM AUC\u2009=\u20090.556). In logistic regression of antibody titers and clinical measures, prediction of LNB was significantly better than the \u201cnull hypothesis\u201d. Clinical measures showed the highest odds ratio.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Data show that in addition to the clinical presentation of patients with symptoms suggesting central or peripheral nervous system manifestation, serum IgG- and IgM-titers help to identify LNB-patients. The results should guide physicians counseling patients with suspected LNB about further diagnostic steps and treatment.<\/jats:p>\n<\/jats:sec>","DOI":"10.1007\/s00415-020-09721-7","type":"journal-article","created":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T13:02:33Z","timestamp":1580562153000},"page":"1476-1481","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Quantitative serological antibody testing for suspected neuroborreliosis"],"prefix":"10.1007","volume":"267","author":[{"given":"Christian","family":"Schneider","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Gielen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"R\u00f6th","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Albrecht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Schroeter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gereon R.","family":"Fink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert","family":"Wunderlich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0674-9891","authenticated-orcid":false,"given":"Helmar C.","family":"Lehmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,1]]},"reference":[{"issue":"8\u201316","key":"9721_CR1","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1111\/j.1468-1331.2009.02862.x","volume":"17","author":"A Mygland","year":"2010","unstructured":"Mygland A, Lj\u00f8stad U, Fingerle V et al (2010) EFNS guidelines on the diagnosis and management of European Lyme neuroborreliosis. 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HCL reports grants and personal fees from Akcea, Alnylam, Biogen, CSL Behring, Grifols, Takeda outside the submitted work. PA reports grants, personal fees and non-financial support from Allergan, Biogen, Ipsen, Merz Pharmaceuticals, Merck, Novartis, and Roche, personal fees and non-financial support from Bayer Healthcare, an non-financial support from Sanofi-Aventis\/Genzyme, outside the submitted work. MS received compensations from Bayer, Biogen, CSL Behring, Genzyme, Grifols, Merck, Miltenyi Biotec, Novartis, Roche, Teva within the last three years. GRF serves as an editorial board member of <i>Cortex, Neurological Research and Practice, NeuroImage: Clinical, Zeitschrift f\u00fcr Neuropsychologie,<\/i> and <i>DGNeurologie<\/i>; receives royalties from the publication of the books <i>Funktionelle MRT in Psychiatrie und Neurologie, Neurologische Differentialdiagnose, and SOP Neurologie<\/i>; received honoraria for speaking engagements from Bayer, Desitin, Ergo DKV, Forum f\u00fcr medizinische Fortbildung FomF GmbH, GSK, Medica Academy Messe D\u00fcsseldorf, Medicbrain Healthcare, Novartis, Pfizer, and Sport\u00e4rztebund NRW.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflicts of interest"}},{"value":"The study was approved by the local ethics committee. The study was carried out in accordance with the Declaration of Helsinki.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical standards"}}]}}