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We assessed agreement between Roche Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      and Fujirebio Lumipulse\n                      <jats:sup>\u00ae<\/jats:sup>\n                      , evaluated Simoa-referenced harmonization, validated Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      clinical associations, and derived directional IVD-to-IVD conversion models between Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      and Lumipulse\n                      <jats:sup>\u00ae<\/jats:sup>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this retrospective real-world cohort (\n                      <jats:italic>N<\/jats:italic>\n                      =253), fasting EDTA plasma was analyzed in singlicate on Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      and Lumipulse\n                      <jats:sup>\u00ae<\/jats:sup>\n                      . Clinical associations of Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      pNfL were tested using multivariable linear models, including age, sex, disease duration, EDSS, cardiovascular comorbidities, smoking, and disease course, with sensitivity analyses including BMI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      On the raw scale, platforms were strongly correlated but showed poor agreement (Coeff 6.91, 95% CI 6.44\u20137.38;\n                      <jats:italic>r<\/jats:italic>\n                      =0.879; CCC=0.107, 95% CI 0.090\u20130.123). After conversion of both assay outputs to published Simoa-equivalent values, alignment improved (Coeff 1.03, 95% CI 0.96\u20131.10). We derived a directional Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      -to-Lumipulse\n                      <jats:sup>\u00ae<\/jats:sup>\n                      Passing\u2013Bablok conversion (Lumipulse = 1.97 + 11.171 \u00d7 Elecsys). Higher Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      pNfL was associated with disability (EDSS: Coeff 0.302, 95% CI 0.122\u20130.481; with BMI: Coeff 0.335, 95% CI 0.119\u20130.551) and was lower in treated versus untreated patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      and Lumipulse\n                      <jats:sup>\u00ae<\/jats:sup>\n                      are not interchangeable on the raw pg\/mL scale though conversion-based approaches can improve cross-platform alignment within the observed analytical range. Elecsys\n                      <jats:sup>\u00ae<\/jats:sup>\n                      pNfL retained expected clinical associations, supporting routine MS use. Findings support cautious conversion during platform transitions in clinics.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00415-026-14068-6","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T09:33:21Z","timestamp":1787650401000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Real-world Roche Elecsys\u00ae and Fujirebio Lumipulse\u00ae inter-platform comparability and clinical validation of plasma neurofilament light chain in multiple sclerosis"],"prefix":"10.1007","volume":"273","author":[{"given":"Vincenzo","family":"Criscuolo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerio","family":"Nicolella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"Polito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Sirica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano","family":"Fiorenza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenica","family":"Sorvillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria Delle","family":"Cave","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Carotenuto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Petracca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Lanzillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Castaldo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo Brescia","family":"Morra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Terracciano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2613-3090","authenticated-orcid":false,"given":"Marcello","family":"Moccia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"issue":"5","key":"14068_CR1","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1038\/s41582-024-00955-x","volume":"20","author":"M Khalil","year":"2024","unstructured":"Khalil M, Teunissen CE, Lehmann S, Otto M, Piehl F, Ziemssen T, Bittner S, Sormani MP, Gattringer T, Abu-Rumeileh S, Thebault S, Abdelhak A, Green A, Benkert P, Kappos L, Comabella M, Tumani H, Freedman MS, Petzold A, Blennow K, Zetterberg H, Leppert D, Kuhle J (2024) Neurofilaments as biomarkers in neurological disorders - towards clinical application. 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