{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:16:18Z","timestamp":1792631778466,"version":"4.5.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2010,2,2]],"date-time":"2010-02-02T00:00:00Z","timestamp":1265068800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Graefes Arch Clin Exp Ophthalmol"],"published-print":{"date-parts":[[2010,6]]},"DOI":"10.1007\/s00417-009-1275-3","type":"journal-article","created":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T07:02:51Z","timestamp":1265007771000},"page":"853-862","source":"Crossref","is-referenced-by-count":36,"title":["Quantification of tear proteins by SDS-PAGE with an internal standard protein: A new method with special reference to small volume tears"],"prefix":"10.1007","volume":"248","author":[{"given":"Kaijun","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaili","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,2,2]]},"reference":[{"key":"1275_CR1","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1002\/1522-2683(200105)22:9<1838::AID-ELPS1838>3.0.CO;2-7","volume":"22","author":"S Herber","year":"2001","unstructured":"Herber S, Grus FH, Sabuncuo P, Augustin AJ (2001) Two-dimensional analysis of tear protein patterns of diabetic patients. Electrophoresis 22:1838\u20131844","journal-title":"Electrophoresis"},{"key":"1275_CR2","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1007\/BF00186580","volume":"232","author":"KB Bjerrum","year":"1994","unstructured":"Bjerrum KB, Prause JU (1994) Collection and concentration of tear proteins studied by SDS gel electrophoresis. Presentation of a new method with special reference to dry eye patients. Graefes Arch Clin Exp Ophthalmol 232:402\u2013405","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"1275_CR3","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1046\/j.1442-9071.2001.00408.x","volume":"29","author":"V Evans","year":"2001","unstructured":"Evans V, Vockler C, Friedlander M, Walsh B, Willcox MD (2001) Lacryglobin in human tears, a potential marker for cancer. Clin Experiment Ophthalmol 29:161\u2013163","journal-title":"Clin Experiment Ophthalmol"},{"key":"1275_CR4","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1007\/PL00007833","volume":"248","author":"D Devos","year":"2001","unstructured":"Devos D, Forzy G, de Seze J, Caillez S, Louchart P, Gallois P, Hautecoeur P (2001) Silver stained isoelectrophoresis of tears and cerebrospinal fluid in multiple sclerosis. J Neurol 248:672\u2013675","journal-title":"J Neurol"},{"key":"1275_CR5","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1021\/pr0498133","volume":"4","author":"BS Koo","year":"2005","unstructured":"Koo BS, Lee DY, Ha HS, Kim JC, Kim CW (2005) Comparative analysis of the tear protein expression in blepharitis patients using two-dimensional electrophoresis. J Proteome Res 4:719\u2013724","journal-title":"J Proteome Res"},{"key":"1275_CR6","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1167\/iovs.08-2604","volume":"50","author":"L Zhou","year":"2009","unstructured":"Zhou L, Beuerman RW, Ang LP, Chan CM, Li SF, Chew FT, Tan DT (2009) Elevation of human alpha-defensins and S100 calcium-binding proteins A8 and A9 in tear fluid of patients with pterygium. Invest Ophthalmol Vis Sci 50:2077\u20132086","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"1275_CR7","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1007\/s004170000140","volume":"238","author":"V Ng","year":"2000","unstructured":"Ng V, Cho P, To C (2000) Tear proteins of normal young Hong Kong Chinese. Graefes Arch Clin Exp Ophthalmol 238:738\u2013745","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"1275_CR8","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1136\/bjo.67.3.199","volume":"67","author":"A Kijlstra","year":"1983","unstructured":"Kijlstra A, Jeurissen SH, Koning KM (1983) Lactoferrin levels in normal human tears. Br J Ophthalmol 67:199\u2013202","journal-title":"Br J Ophthalmol"},{"key":"1275_CR9","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1136\/bjo.68.5.316","volume":"68","author":"JI McGill","year":"1984","unstructured":"McGill JI, Liakos GM, Goulding N, Seal DV (1984) Normal tear protein profiles and age-related changes. Br J Ophthalmol 68:316\u2013320","journal-title":"Br J Ophthalmol"},{"key":"1275_CR10","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1111\/j.1755-3768.1994.tb02773.x","volume":"72","author":"TR Stolwijk","year":"1994","unstructured":"Stolwijk TR, Kuizenga A, van Haeringen NJ, Kijlstra A, Oosterhuis JA, van Best JA (1994) Analysis of tear fluid proteins in insulin-dependent diabetes mellitus. Acta Ophthalmol (Copenh) 72:357\u2013362","journal-title":"Acta Ophthalmol (Copenh)"},{"key":"1275_CR11","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.1002\/1522-2683(200207)23:14<2203::AID-ELPS2203>3.0.CO;2-H","volume":"23","author":"JC Nishihara","year":"2002","unstructured":"Nishihara JC, Champion KM (2002) Quantitative evaluation of proteins in one- and two-dimensional polyacrylamide gels using a fluorescent stain. Electrophoresis 23:2203\u20132215","journal-title":"Electrophoresis"},{"key":"1275_CR12","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1002\/prca.200700125","volume":"1","author":"K Wu","year":"2007","unstructured":"Wu K , Zhang Y (2007) Clinical application of tear proteomics: present and future prospects. Proteomics Clin Appl 1:972\u2013982","journal-title":"Proteomics Clin Appl"},{"key":"1275_CR13","first-page":"44","volume-title":"Dry Eye and Ocular Surface Disorders","author":"ME Stern","year":"2004","unstructured":"Stern ME, Beuerman RW, Pflugfelder SC (2004) The Normal Tear Film and Ocular Surface. In: Pflugfelder SC, Beuerman RW, Stern ME (eds) Dry Eye and Ocular Surface Disorders. Marcel Dekker, New York, pp 44\u201349"},{"key":"1275_CR14","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1039\/b701541k","volume":"132","author":"J Filik","year":"2007","unstructured":"Filik J, Stone N (2007) Drop coating deposition Raman spectroscopy of protein mixtures. Analyst 132:544\u2013550","journal-title":"Analyst"},{"key":"1275_CR15","first-page":"1119","volume":"31","author":"RJ Fullard","year":"1990","unstructured":"Fullard RJ, Snyder C (1990) Protein levels in nonstimulated and stimulated tears of normal human subjects. Invest Ophthalmol Vis Sci 31:1119\u20131126","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"1275_CR16","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/BF02173448","volume":"219","author":"A Berta","year":"1982","unstructured":"Berta A (1982) A polyacrylamide-gel electrophoretic study of human tear proteins. Graefes Arch Clin Exp Ophthalmol 219:95\u201399","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"1275_CR17","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/0014-4835(79)90154-4","volume":"29","author":"AM Gachon","year":"1979","unstructured":"Gachon AM, Verrelle P, Betail G, Dastugue B (1979) Immunological and electrophoretic studies of human tear proteins. Exp Eye Res 29:539\u2013553","journal-title":"Exp Eye Res"},{"key":"1275_CR18","first-page":"381","volume":"32","author":"A Kuizenga","year":"1991","unstructured":"Kuizenga A, van Haeringen NJ, Kijlstra A (1991) SDS-Minigel electrophoresis of human tears. Effect of sample treatment on protein patterns. Invest Ophthalmol Vis Sci 32:381\u2013386","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"1275_CR19","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1021\/pr0501970","volume":"4","author":"N Li","year":"2005","unstructured":"Li N, Wang N, Zheng J, Liu XM, Lever OW, Erickson PM, Li L (2005) Characterization of human tear proteome using multiple proteomic analysis techniques. J Proteome Res 4:2052\u20132061","journal-title":"J Proteome Res"},{"key":"1275_CR20","first-page":"456","volume":"14","author":"KB Green-Church","year":"2008","unstructured":"Green-Church KB, Nichols KK, Kleinholz NM, Zhang L, Nichols JJ (2008) Investigation of the human tear film proteome using multiple proteomic approaches. Mol Vis 14:456\u2013470","journal-title":"Mol Vis"},{"key":"1275_CR21","doi-asserted-by":"crossref","first-page":"426","DOI":"10.2144\/00283bm07","volume":"28","author":"C Wong","year":"2000","unstructured":"Wong C, Sridhara S, Bardwell JC, Jakob U (2000) Heating greatly speeds Coomassie blue staining and destaining. Biotechniques 28:426\u2013428, 430, 432","journal-title":"Biotechniques"},{"key":"1275_CR22","first-page":"560","volume":"27","author":"N Jiang","year":"2000","unstructured":"Jiang N, Huang Q, Kl Wu, Pan SH (2000) A simple method of protein staining with CBB G250. Prog Biochem Biophys 27:560\u2013561","journal-title":"Prog Biochem Biophys"},{"key":"1275_CR23","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/j.1600-0420.1997.tb00138.x","volume":"75","author":"KB Bjerrum","year":"1997","unstructured":"Bjerrum KB (1997) The ratio of albumin to lactoferrin in tear fluid as a diagnostic tool in primary Sjogren\u2019s syndrome. Acta Ophthalmol Scand 75:507\u2013511","journal-title":"Acta Ophthalmol Scand"},{"key":"1275_CR24","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1002\/1522-2683(200105)22:9<1845::AID-ELPS1845>3.0.CO;2-N","volume":"22","author":"FH Grus","year":"2001","unstructured":"Grus FH, Sabuncuo P, Augustin AJ (2001) Analysis of tear protein patterns of dry-eye patients using fluorescent staining dyes and two-dimensional quantification algorithms. Electrophoresis 22:1845\u20131850","journal-title":"Electrophoresis"},{"key":"1275_CR25","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1177\/112067219800800207","volume":"8","author":"FH Grus","year":"1998","unstructured":"Grus FH, Augustin AJ, Evangelou NG, Toth-Sagi K (1998) Analysis of tear-protein patterns as a diagnostic tool for the detection of dry eyes. Eur J Ophthalmol 8:90\u201397","journal-title":"Eur J Ophthalmol"},{"key":"1275_CR26","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1021\/pr700589s","volume":"7","author":"S Schroder","year":"2008","unstructured":"Schroder S, Zhang H, Yeung ES, Jansch L, Zabel C, Watzig H (2008) Quantitative gel electrophoresis: sources of variation. J Proteome Res 7:1226\u20131234","journal-title":"J Proteome Res"},{"key":"1275_CR27","doi-asserted-by":"crossref","first-page":"897","DOI":"10.3109\/02713688409167207","volume":"3","author":"FK Holly","year":"1984","unstructured":"Holly FK, LauKaitis SJ, Esquivel ED (1984) Kinetics of lacrimal secretions in normal human subject. Curr Eye Res 3:897\u2013910","journal-title":"Curr Eye Res"},{"key":"1275_CR28","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248\u2013254","journal-title":"Anal Biochem"},{"key":"1275_CR29","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680\u2013685","journal-title":"Nature"},{"key":"1275_CR30","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1097\/00003226-199413060-00007","volume":"13","author":"CJ McCollum","year":"1994","unstructured":"McCollum CJ, Foulks GN, Bodner B, Shepard J, Daniels K, Gross V, Kelly L, Cavanagh HD (1994) Rapid assay of lactoferrin in keratoconjunctivitis sicca. Cornea 13:505\u2013508","journal-title":"Cornea"},{"key":"1275_CR31","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1001\/archopht.1974.03900060446002","volume":"91","author":"OP Bijsterveld van","year":"1974","unstructured":"van Bijsterveld OP (1974) Standardization of the lysozyme test for a commercially available medium. Its use for the diagnosis of the sicca syndrome. Arch Ophthalmol 91:432\u2013434","journal-title":"Arch Ophthalmol"},{"key":"1275_CR32","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1097\/01.ico.0000183531.25201.0d","volume":"25","author":"J Lopez-Cisternas","year":"2006","unstructured":"Lopez-Cisternas J, Castillo-Diaz J, Traipe-Castro L, Lopez-Solis RO (2006) Use of polyurethane minisponges to collect human tear fluid. Cornea 25:312\u2013318","journal-title":"Cornea"},{"key":"1275_CR33","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1002\/(SICI)1522-2683(19990101)20:4\/5<875::AID-ELPS875>3.0.CO;2-V","volume":"20","author":"FH Grus","year":"1999","unstructured":"Grus FH, Augustin AJ (1999) Analysis of tear protein patterns by a neural network as a diagnostical tool for the detection of dry eyes. Electrophoresis 20:875\u2013880","journal-title":"Electrophoresis"},{"key":"1275_CR34","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0003-2697(81)90321-3","volume":"116","author":"SM Read","year":"1981","unstructured":"Read SM, Northcote DH (1981) Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem 116:53\u201364","journal-title":"Anal Biochem"},{"key":"1275_CR35","first-page":"418","volume-title":"The precorneal tear film in health, disease and contact lens wear","author":"Berta","year":"1986","unstructured":"Berta (1986) Standardization of tear protein determinations: the effects of sampling, flow rate, and vascular permeability. In: FJ H (ed) The precorneal tear film in health, disease and contact lens wear. Dry Eye Institute, Lubbock, TX, pp 418\u2013435"},{"key":"1275_CR36","first-page":"374","volume":"25","author":"RN Stuchell","year":"1984","unstructured":"Stuchell RN, Feldman JJ, Farris RL, Mandel ID (1984) The effect of collection technique on tear composition. Invest Ophthalmol Vis Sci 25:374\u2013377","journal-title":"Invest Ophthalmol Vis Sci"}],"container-title":["Graefe's Archive for Clinical and Experimental Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00417-009-1275-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00417-009-1275-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00417-009-1275-3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T15:40:52Z","timestamp":1559058052000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00417-009-1275-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,2]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["1275"],"URL":"https:\/\/doi.org\/10.1007\/s00417-009-1275-3","relation":{},"ISSN":["0721-832X","1435-702X"],"issn-type":[{"value":"0721-832X","type":"print"},{"value":"1435-702X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2,2]]}}}