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The graft endothelial cell loss, endothelial graft thickness, visual acuity, and complications after surgery were evaluated.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Thin endothelial grafts were all successfully prepared, with no occurrence of perforation. The rate of endothelial cell loss was 17.4%, 18.8%, 19.9%, and 26.7%, and the central graft thickness was 113\u00b154 \u03bcm, 102\u00b140 \u03bcm, 101\u00b128 \u03bcm, and 96\u00b123 \u03bcm at 3, 6, 12, and 24 months, respectively. The median best-corrected visual acuity was 0.4 logMAR (range, 0\u20132 logMAR) at 24 months, demonstrating a significant difference from that before surgery (2 logMAR; range, 0.2\u20133 logMAR) (<jats:italic>T<\/jats:italic>=187.5, <jats:italic>P<\/jats:italic>&lt;.001). Partial graft dislocation was the most common postoperative complication, with an occurrence rate of 14% (<jats:italic>n<\/jats:italic>=12), and it was associated with an abnormal iris-lens diaphragm (<jats:italic>r<\/jats:italic>=.35, <jats:italic>P<\/jats:italic>&lt;.001). The other complications included a high intraocular pressure (<jats:italic>n<\/jats:italic>=5, 6%), endothelial graft rejection (<jats:italic>n<\/jats:italic>=4, 5%), and pupillary block (<jats:italic>n<\/jats:italic>=1, 1%). Endothelial graft decompensation occurred in the two eyes, and 98% (<jats:italic>n<\/jats:italic>=83) of the grafts survived at 24 months.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Data of the study suggest that the treatment using FLS-DSEK seems to be promising and might be considered a feasible choice in patients with endothelial dysfunction.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Trial registration<\/jats:title>\n                <jats:p>1. Date of registration: 2021-02-18<\/jats:p>\n                <jats:p>2. Trial registration number: ChiCTR2100044091<\/jats:p>\n                <jats:p>3. Registration site: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.chictr.org.cn\/\">https:\/\/www.chictr.org.cn\/<\/jats:ext-link><\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00417-021-05383-x","type":"journal-article","created":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T05:02:47Z","timestamp":1630386167000},"page":"181-189","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Femtosecond laser semi-assisted Descemet stripping endothelial keratoplasty: 2-year outcomes of endothelial cell loss and graft survival"],"prefix":"10.1007","volume":"260","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6569-4395","authenticated-orcid":false,"given":"Ting","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peiyan","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengjie","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1326-6946","authenticated-orcid":false,"given":"Hua","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suxia","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4106-373X","authenticated-orcid":false,"given":"Weiyun","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,8,31]]},"reference":[{"issue":"1","key":"5383_CR1","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1097\/ICO.0b013e31825c72dc","volume":"32","author":"PM Phillips","year":"2013","unstructured":"Phillips PM, Phillips LJ, Saad HA, Terry MA, Stolz DB, Stoeger C, Franks J, Davis-Boozer D (2013) \u201cUltrathin\u201d DSAEK tissue prepared with a low-pulse energy, high-frequency femtosecond laser. 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