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Perimetry, optical coherence tomography (OCT), and OCT angiography were performed. Multispectral imaging was used to record the optic disc reflectance at wavelengths 522\u00a0nm, 548\u00a0nm, 555\u00a0nm, 586\u00a0nm, and 610\u00a0nm, and haemoglobin concentration and oxygenation (SO<jats:sub>2<\/jats:sub>) were calculated from these measures. This was done in the rest and under stimulation of neuronal activity by flicker light.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The haemoglobin concentration was significantly lower (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) in the rim (40.0\u2009\u00b1\u20096.3) and the excavation (35.7\u2009\u00b1\u20098.0) of the glaucoma patients\u2019 discs than in controls (45.7\u2009\u00b1\u20097.5). SO<jats:sub>2<\/jats:sub> was not different in general, but lower in a subgroup of 18 glaucoma patients with ischaemic disc rims than in non-ischaemic ones (median 26.8%, interquartile range (IQR): 29.5% vs. 51.9%, IQR 32.0%, <jats:italic>p<\/jats:italic>\u2009=\u20090.02) as well as in controls (41.0%, IQR 30.6%, <jats:italic>p<\/jats:italic>\u2009=\u20090.01). Flicker light stimulation significantly increased the haemoglobin concentration in the controls (+\u20091.3\u2009\u00b1\u20093.6, <jats:italic>p<\/jats:italic>\u2009=\u20090.048) as well as in the rim of glaucoma discs (+\u20092.6\u2009\u00b1\u20095.0, <jats:italic>p<\/jats:italic>\u2009=\u20090.006) and SO<jats:sub>2<\/jats:sub> in the controls only (+\u200915.4\u2009\u00b1\u200923.6%, <jats:italic>p<\/jats:italic>\u2009=\u20090.001). The haemoglobin concentration was significantly correlated with the perimetric mean defect, retinal nerve fibre layer (RNFL) thickness and para-papillary perfusion density.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The optic disc haemoglobin concentration and oxygenation are quantifiable from multispectral imaging and reduced in glaucoma. The correlation of haemoglobin concentration with perfusion density, RNFL thickness and visual field loss indicates its implication in glaucoma pathology.<\/jats:p>\n                \n              <\/jats:sec>","DOI":"10.1007\/s00417-022-05722-6","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T08:03:03Z","timestamp":1654502583000},"page":"3587-3595","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Optic disc blood perfusion and oxygenation in glaucoma"],"prefix":"10.1007","volume":"260","author":[{"given":"Hosni","family":"Al Zoubi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Riemer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rowena","family":"Simon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walthard","family":"Vilser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somar","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Meller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regine","family":"Augsten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9674-8096","authenticated-orcid":false,"given":"Martin","family":"Hammer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,6]]},"reference":[{"key":"5722_CR1","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1136\/bjo.56.3.175","volume":"56","author":"SS Hayreh","year":"1972","unstructured":"Hayreh SS (1972) Optic disc changes in glaucoma. 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