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Molecular analyses of granulosa cell-associated processes, leading to improvement of understanding of the cell cycle events during the formation of ovarian follicles (folliculogenesis), may be key to improve the in vitro fertilization procedures. Primary in vitro culture of porcine GCs was employed to examine the changes in the transcriptomic profile of genes belonging to \u201ccell cycle\u201d, \u201ccell division\u201d, \u201ccell cycle process\u201d, \u201ccell cycle phase transition\u201d, \u201ccell cycle G1\/S phase transition\u201d, \u201ccell cycle G2\/M phase transition\u201d and \u201ccell cycle checkpoint\u201d ontology groups. During the analysis, microarrays were employed to study the transcriptome of GCs, analyzing the total RNA of cells from specific periods of in vitro cultures. This research was based on material obtained from 40 landrace gilts of similar weight, age and the same living conditions. RNA was isolated at specific timeframes: before the culture was established (0\u00a0h) and after 48\u00a0h, 96\u00a0h and 144\u00a0h in vitro. Out of 133 differentially expressed genes, we chose the 10 most up-regulated (<jats:italic>SFRP2<\/jats:italic>, <jats:italic>PDPN<\/jats:italic>, <jats:italic>PDE3A<\/jats:italic>, <jats:italic>FGFR2<\/jats:italic>, <jats:italic>PLK2<\/jats:italic>, <jats:italic>THBS1<\/jats:italic>, <jats:italic>ETS1<\/jats:italic>, <jats:italic>LIF<\/jats:italic>, <jats:italic>ANXA1<\/jats:italic>, <jats:italic>TGFB1<\/jats:italic>) and the 10 most downregulated (<jats:italic>IGF1<\/jats:italic>, <jats:italic>NCAPD2<\/jats:italic>, <jats:italic>CABLES1<\/jats:italic>, <jats:italic>H1FOO<\/jats:italic>, <jats:italic>NEK2<\/jats:italic>, <jats:italic>PPAT<\/jats:italic>, <jats:italic>TXNIP<\/jats:italic>, <jats:italic>NUP210<\/jats:italic>, <jats:italic>RGS2<\/jats:italic> and <jats:italic>CCNE2<\/jats:italic>). Some of these genes known to play key roles in the regulation of correct cell cycle passage (up-regulated SFRP2, PDE3A, PLK2, LIF and down-regulated CCNE2, TXNIP, NEK2). The data obtained provide a potential reference for studies on the process of mammalian folliculogenesis, as well as suggests possible new genetic markers for cell cycle progress in in vitro cultured porcine granulosa cells.<\/jats:p>","DOI":"10.1007\/s00418-020-01860-2","type":"journal-article","created":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T02:25:22Z","timestamp":1593483922000},"page":"397-412","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Transcriptomic analysis of expression of genes regulating cell cycle progression in porcine ovarian granulosa cells during short-term in vitro primary culture"],"prefix":"10.1007","volume":"153","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1098-5627","authenticated-orcid":false,"given":"Magdalena","family":"Kulus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7518-4834","authenticated-orcid":false,"given":"Wies\u0142awa","family":"Kranc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0077-3236","authenticated-orcid":false,"given":"Patrycja","family":"Sujka-Kordowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8211-7664","authenticated-orcid":false,"given":"Piotr","family":"Celichowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2109-3042","authenticated-orcid":false,"given":"Aneta","family":"Konwerska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9476-0235","authenticated-orcid":false,"given":"Maurycy","family":"Jankowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1778-3454","authenticated-orcid":false,"given":"Michal","family":"Jeseta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2775-9642","authenticated-orcid":false,"given":"Mariusz T.","family":"Skowro\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8904-2885","authenticated-orcid":false,"given":"Hanna","family":"Piotrowska-Kempisty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1835-4483","authenticated-orcid":false,"given":"Dorota","family":"Bukowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2004-0010","authenticated-orcid":false,"given":"Maciej","family":"Zabel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2899-3943","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Bruska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1575-3123","authenticated-orcid":false,"given":"Paul","family":"Mozdziak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6804-0119","authenticated-orcid":false,"given":"Bartosz","family":"Kempisty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4835-2825","authenticated-orcid":false,"given":"Pawe\u0142","family":"Antosik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,10]]},"reference":[{"key":"1860_CR1","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1038\/nrm3629","volume":"14","author":"C Bertoli","year":"2013","unstructured":"Bertoli C, Skotheim JM, de Bruin RAM (2013) Control of cell cycle transcription during G1 and S phases. 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