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We found that fetal corneas at 20\u00a0weeks of gestation (wg) and normal adult corneas showed similar staining patterns for Notch1; however 10\u201311\u00a0wg fetal corneas showed increased presence of Notch1. Numb and Dlk1 had an enhanced presence in the fetal corneas compared with the adult corneas. Fetal corneas showed stronger immunolabeling with antibodies against \u03b2-catenin, Wnt5a, Wnt7a, Gli1, Hes1, p-rpS6, and mTOR when compared with the adult corneas. Gene expression of Notch1, Wnt5A, Wnt7A, \u03b2-catenin, Hes1, mTOR, and rps6 was higher in the 9\u201312\u00a0wg fetal corneas compared with adult corneas. The cell signaling pathway differences found between human fetal and adult corneas were similar to those previously found in ARK corneas with the exception of Notch1. Analogous profiles of cell signaling pathway activation between human fetal corneas and ARK corneas suggests that there is a less differentiated host milieu in ARK.<\/jats:p>","DOI":"10.1007\/s00418-022-02099-9","type":"journal-article","created":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T06:21:04Z","timestamp":1652336464000},"page":"169-180","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Aniridia-related keratopathy relevant cell signaling pathways in human fetal corneas"],"prefix":"10.1007","volume":"158","author":[{"given":"Andr\u00e9","family":"Vicente","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8039-493X","authenticated-orcid":false,"given":"Marta","family":"Sloniecka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-Xia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berit","family":"Bystr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1tima","family":"Pedrosa Domell\u00f6f","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,5,12]]},"reference":[{"issue":"5","key":"2099_CR1","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1359\/JBMR.040118","volume":"19","author":"BM Abdallah","year":"2004","unstructured":"Abdallah BM, Jensen CH, Gutierrez G, Leslie RG, Jensen TG, Kassem M (2004) Regulation of human skeletal stem cells differentiation by Dlk1\/Pref-1. 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The fetal samples were collected with ethical approval after legal interruptions of pregnancy. The ARK samples were collected during surgery, with the approval of the Regional Ethical Commitees of Ume\u00e5 and Gothenburg, following informed consent. The study followed the principles of the Declaration of Helsinki.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}