{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T07:10:52Z","timestamp":1785222652809,"version":"3.55.0"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG KI 728\/12-1"],"award-info":[{"award-number":["DFG KI 728\/12-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Medizinische Hochschule Hannover (MHH)"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Histochem Cell Biol"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The urothelium is a stratified epithelium that lines the inner surface of the components of the urinary drainage system. It is composed of a layer of basal cells, one or several layers of intermediate cells, and a layer of large luminal superficial or umbrella cells. In the mouse, only a small set of markers is available that allows easy molecular distinction of these urothelial cell types. Here, we analyzed expression of S100A1, a member of the S100 family of calcium-binding proteins, in the urothelium of the two major organs of the murine urinary tract, the ureter and the bladder. Using RNA in situ hybridization analysis, we found exclusive expression of <jats:italic>S100a1<\/jats:italic> mRNA in luminal cells of the ureter from embryonic day (E)17.5 onwards and of the bladder from E15.5 to adulthood. Immunofluorescence analysis showed that expression of S100A1 protein is confined to terminally differentiated superficial cells of both the ureter and bladder where it localized to the nucleus and cytoplasm. We conclude that S100A1 is a suitable marker for mature superficial cells in the urothelial lining of the drainage system of the developing and mature mouse.<\/jats:p>","DOI":"10.1007\/s00418-022-02120-1","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T05:02:31Z","timestamp":1654059751000},"page":"389-399","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["S100A1 expression characterizes terminally differentiated superficial cells in the urothelium of the murine bladder and ureter"],"prefix":"10.1007","volume":"158","author":[{"given":"Fairouz","family":"Qasrawi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Meuser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Finja","family":"Lehnhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjenna","family":"Schulte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8154-0257","authenticated-orcid":false,"given":"Andreas","family":"Kispert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,1]]},"reference":[{"key":"2120_CR1","doi-asserted-by":"publisher","first-page":"F305","DOI":"10.1152\/ajprenal.00341.2003","volume":"287","author":"P Acharya","year":"2004","unstructured":"Acharya P, Beckel J, Ruiz WG, Wang E, Rojas R, Birder L, Apodaca G (2004) Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium. Am J Physiol Renal Physiol 287:F305-318. https:\/\/doi.org\/10.1152\/ajprenal.00341.2003","journal-title":"Am J Physiol Renal Physiol"},{"key":"2120_CR2","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.aanat.2014.11.001","volume":"198","author":"S Arrighi","year":"2015","unstructured":"Arrighi S (2015) The urothelium: anatomy, review of the literature, perspectives for veterinary medicine. Ann Anat 198:73\u201382. https:\/\/doi.org\/10.1016\/j.aanat.2014.11.001","journal-title":"Ann Anat"},{"key":"2120_CR3","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1002\/cne.20944","volume":"496","author":"O Bermingham-McDonogh","year":"2006","unstructured":"Bermingham-McDonogh O, Oesterle EC, Stone JS, Hume CR, Huynh HM, Hayashi T (2006) Expression of prox1 during mouse cochlear development. J Comp Neurol 496:172\u2013186. https:\/\/doi.org\/10.1002\/cne.20944","journal-title":"J Comp Neurol"},{"key":"2120_CR4","doi-asserted-by":"publisher","first-page":"1792","DOI":"10.1681\/ASN.2016080849","volume":"28","author":"T Bohnenpoll","year":"2017","unstructured":"Bohnenpoll T, Feraric S, Nattkemper M, Weiss AC, Rudat C, Meuser M, Trowe MO, Kispert A (2017) Diversification of cell lineages in ureter development. J Am Soc Nephrol 28:1792\u20131801. https:\/\/doi.org\/10.1681\/ASN.2016080849","journal-title":"J Am Soc Nephrol"},{"key":"2120_CR5","doi-asserted-by":"publisher","first-page":"1031","DOI":"10.1097\/PAS.0b013e31819c6ff9","volume":"33","author":"P Cossu-Rocca","year":"2009","unstructured":"Cossu-Rocca P, Contini M, Brunelli M, Festa A, Pili F, Gobbo S, Eccher A, Mura A, Massarelli G, Martignoni G (2009) S-100A1 is a reliable marker in distinguishing nephrogenic adenoma from prostatic adenocarcinoma. Am J Surg Pathol 33:1031\u20131036. https:\/\/doi.org\/10.1097\/PAS.0b013e31819c6ff9","journal-title":"Am J Surg Pathol"},{"key":"2120_CR6","doi-asserted-by":"publisher","first-page":"1675","DOI":"10.1242\/dev.060210","volume":"138","author":"MJ Croyle","year":"2011","unstructured":"Croyle MJ, Lehman JM, O\u2019Connor AK, Wong SY, Malarkey EB, Iribarne D, Dowdle WE, Schoeb TR, Verney ZM, Athar M, Michaud EJ, Reiter JF, Yoder BK (2011) Role of epidermal primary cilia in the homeostasis of skin and hair follicles. Development 138:1675\u20131685. https:\/\/doi.org\/10.1242\/dev.060210","journal-title":"Development"},{"key":"2120_CR7","doi-asserted-by":"publisher","first-page":"1621","DOI":"10.1152\/physrev.00041.2019","volume":"100","author":"MG Dalghi","year":"2020","unstructured":"Dalghi MG, Montalbetti N, Carattino MD, Apodaca G (2020) The urothelium: life in a liquid environment. Physiol Rev 100:1621\u20131705. https:\/\/doi.org\/10.1152\/physrev.00041.2019","journal-title":"Physiol Rev"},{"key":"2120_CR8","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1309\/AJCPTK87EMMIKPFS","volume":"132","author":"MS DeRycke","year":"2009","unstructured":"DeRycke MS, Andersen JD, Harrington KM, Pambuccian SE, Kalloger SE, Boylan KL, Argenta PA, Skubitz AP (2009) S100A1 expression in ovarian and endometrial endometrioid carcinomas is a prognostic indicator of relapse-free survival. Am J Clin Pathol 132:846\u2013856. https:\/\/doi.org\/10.1309\/AJCPTK87EMMIKPFS","journal-title":"Am J Clin Pathol"},{"key":"2120_CR9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000582","volume":"9","author":"G Diez-Roux","year":"2011","unstructured":"Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand ESS, N\u00fcrnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Doll\u00e9 P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A (2011) A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol 9:e1000582. https:\/\/doi.org\/10.1371\/journal.pbio.1000582","journal-title":"PLoS Biol"},{"key":"2120_CR10","doi-asserted-by":"publisher","first-page":"2821","DOI":"10.1128\/MCB.22.8.2821-2829.2002","volume":"22","author":"XJ Du","year":"2002","unstructured":"Du XJ, Cole TJ, Tenis N, Gao XM, K\u00f6ntgen F, Kemp BE, Heierhorst J (2002) Impaired cardiac contractility response to hemodynamic stress in S100A1-deficient mice. Mol Cell Biol 22:2821\u20132829. https:\/\/doi.org\/10.1128\/MCB.22.8.2821-2829.2002","journal-title":"Mol Cell Biol"},{"key":"2120_CR11","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.749052","volume":"8","author":"P Ecs\u00e9di","year":"2021","unstructured":"Ecs\u00e9di P, G\u00f3gl G, Nyitray L (2021) Studying the structures of relaxed and fuzzy interactions: the diverse world of S100 complexes. Front Mol Biosci 8:749052. https:\/\/doi.org\/10.3389\/fmolb.2021.749052","journal-title":"Front Mol Biosci"},{"key":"2120_CR12","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1007\/s004180000240","volume":"115","author":"A Erman","year":"2001","unstructured":"Erman A, Jezernik K, Stiblar-Martincic D, Romih R, Veranic P (2001) Postnatal restoration of the mouse urinary bladder urothelium. Histochem Cell Biol 115:309\u2013316. https:\/\/doi.org\/10.1007\/s004180000240","journal-title":"Histochem Cell Biol"},{"key":"2120_CR13","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/j.devcel.2013.07.017","volume":"26","author":"D Gandhi","year":"2013","unstructured":"Gandhi D, Molotkov A, Batourina E, Schneider K, Dan H, Reiley M, Laufer E, Metzger D, Liang F, Liao Y, Sun TT, Aronow B, Rosen R, Mauney J, Adam R, Rosselot C, Van Batavia J, McMahon A, McMahon J, Guo JJ, Mendelsohn C (2013) Retinoid signaling in progenitors controls specification and regeneration of the urothelium. Dev Cell 26:469\u2013482. https:\/\/doi.org\/10.1016\/j.devcel.2013.07.017","journal-title":"Dev Cell"},{"key":"2120_CR14","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/S0002-9440(10)63306-8","volume":"165","author":"K Hibbs","year":"2004","unstructured":"Hibbs K, Skubitz KM, Pambuccian SE, Casey RC, Burleson KM, Oegema TRJ, Thiele JJ, Grindle SM, Bliss RL, Skubitz AP (2004) Differential gene expression in ovarian carcinoma: identification of potential biomarkers. Am J Pathol 165:397\u2013414. https:\/\/doi.org\/10.1016\/S0002-9440(10)63306-8","journal-title":"Am J Pathol"},{"key":"2120_CR15","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1097\/01.pas.0000213438.01278.5f","volume":"31","author":"JP Higgins","year":"2007","unstructured":"Higgins JP, Kaygusuz G, Wang L, Montgomery K, Mason V, Zhu SX, Marinelli RJ, Presti JCJ, van de Rijn M, Brooks JD (2007) Placental S100 (S100P) and GATA3: markers for transitional epithelium and urothelial carcinoma discovered by complementary DNA microarray. Am J Surg Pathol 31:673\u2013680. https:\/\/doi.org\/10.1097\/01.pas.0000213438.01278.5f","journal-title":"Am J Surg Pathol"},{"key":"2120_CR16","doi-asserted-by":"publisher","first-page":"F1200","DOI":"10.1152\/ajprenal.00043.2002","volume":"283","author":"P Hu","year":"2002","unstructured":"Hu P, Meyers S, Liang FX, Deng FM, Kachar B, Zeidel ML, Sun TT (2002) Role of membrane proteins in permeability barrier function: uroplakin ablation elevates urothelial permeability. Am J Physiol Renal Physiol 283:F1200-1207. https:\/\/doi.org\/10.1152\/ajprenal.00043.2002","journal-title":"Am J Physiol Renal Physiol"},{"key":"2120_CR17","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/s0167-4889(00)00097-5","volume":"1498","author":"R Kiewitz","year":"2000","unstructured":"Kiewitz R, Lyons GE, Sch\u00e4fer BW, Heizmann CW (2000) Transcriptional regulation of S100A1 and expression during mouse heart development. Biochem Biophys Acta 1498:207\u2013219. https:\/\/doi.org\/10.1016\/s0167-4889(00)00097-5","journal-title":"Biochem Biophys Acta"},{"key":"2120_CR18","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1159\/000092049","volume":"76","author":"M Lazzeri","year":"2006","unstructured":"Lazzeri M (2006) The physiological function of the urothelium\u2013more than a simple barrier. Urol Int 76:289\u2013295. https:\/\/doi.org\/10.1159\/000092049","journal-title":"Urol Int"},{"key":"2120_CR19","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1016\/j.bbrc.2004.07.096","volume":"322","author":"I Marenholz","year":"2004","unstructured":"Marenholz I, Heizmann CW, Fritz G (2004) S100 proteins in mouse and man: from evolution to function and pathology (including an update of the nomenclature). Biochem Biophys Res Commun 322:1111\u20131122. https:\/\/doi.org\/10.1016\/j.bbrc.2004.07.096","journal-title":"Biochem Biophys Res Commun"},{"key":"2120_CR20","first-page":"41","volume":"29","author":"G Martignoni","year":"2007","unstructured":"Martignoni G, Brunelli M, Gobbo S, Remo A, Ficarra V, Cossu-Rocca P, Pea M, Chilosi M, Menestrina F, Cheng L (2007) Role of molecular markers in diagnosis and prognosis of renal cell carcinoma. Anal Quant Cytol Histol 29:41\u201349","journal-title":"Anal Quant Cytol Histol"},{"key":"2120_CR21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003441","volume":"3","author":"A Medawar","year":"2008","unstructured":"Medawar A, Virolle T, Rostagno P, de la Forest-Divonne S, Gambaro K, Rouleau M, Aberdam D (2008) DeltaNp63 is essential for epidermal commitment of embryonic stem cells. PLoS One 3:e3441. https:\/\/doi.org\/10.1371\/journal.pone.0003441","journal-title":"PLoS One"},{"key":"2120_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1177\/002215540104900101","volume":"49","author":"AF Moorman","year":"2001","unstructured":"Moorman AF, Houweling AC, de Boer PA, Christoffels VM (2001) Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol. J Histochem Cytochem 49:1\u20138. https:\/\/doi.org\/10.1177\/002215540104900101","journal-title":"J Histochem Cytochem"},{"key":"2120_CR23","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1161\/CIRCRESAHA.108.172031","volume":"102","author":"ST Pleger","year":"2008","unstructured":"Pleger ST, Harris DM, Shan C, Vinge LE, Chuprun JK, Berzins B, Pleger W, Druckman C, V\u00f6lkers M, Heierhorst J, \u00d8ie E, Remppis A, Katus HA, Scalia R, Eckhart AD, Koch WJ, Most P (2008) Endothelial S100A1 modulates vascular function via nitric oxide. Circ Res 102:786\u2013794. https:\/\/doi.org\/10.1161\/CIRCRESAHA.108.172031","journal-title":"Circ Res"},{"key":"2120_CR24","doi-asserted-by":"publisher","first-page":"5046","DOI":"10.1074\/jbc.M709231200","volume":"283","author":"B Prosser","year":"2008","unstructured":"Prosser B, Wright NT, Hern\u00e3ndez-Ochoa EO, Varney KM, Liu Y, Olojo RO, Zimmer D, Weber DJ, Schneider MF (2008) S100A1 binds to the calmodulin-binding site of ryanodine receptor and modulates skeletal muscle excitation-contraction coupling. J Biol Chem 283:5046\u20135057. https:\/\/doi.org\/10.1074\/jbc.M709231200","journal-title":"J Biol Chem"},{"key":"2120_CR25","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/sj.embor.7400934","volume":"8","author":"T Saito","year":"2007","unstructured":"Saito T, Ikeda T, Nakamura K, Chung UI, Kawaguchi H (2007) S100A1 and S100B, transcriptional targets of SOX trio, inhibit terminal differentiation of chondrocytes. EMBO Rep 8:504\u2013509. https:\/\/doi.org\/10.1038\/sj.embor.7400934","journal-title":"EMBO Rep"},{"key":"2120_CR26","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/BF00357068","volume":"23","author":"TT Sun","year":"1996","unstructured":"Sun TT, Zhao H, Provet J, Aebi U, Wu XR (1996) Formation of asymmetric unit membrane during urothelial differentiation. Mol Biol Rep 23:3\u201311","journal-title":"Mol Biol Rep"},{"key":"2120_CR27","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1097\/PAP.0000000000000150","volume":"24","author":"M Suryavanshi","year":"2017","unstructured":"Suryavanshi M, Sanz-Ortega J, Sirohi D, Divatia MK, Ohe C, Zampini C, Luthringer D, Smith SC, Amin MB (2017) S100P as a marker for urothelial histogenesis: a critical review and comparison with novel and traditional urothelial immunohistochemical markers. Adv Anat Pathol 24:151\u2013160. https:\/\/doi.org\/10.1097\/PAP.0000000000000150","journal-title":"Adv Anat Pathol"},{"key":"2120_CR28","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1093\/cvr\/cvu241","volume":"105","author":"K Teichert-Kuliszewska","year":"2015","unstructured":"Teichert-Kuliszewska K, Tsoporis JN, Desjardins JF, Yin J, Wang L, Kuebler WM, Parker TG (2015) Absence of the calcium-binding protein, S100A1, confers pulmonary hypertension in mice associated with endothelial dysfunction and apoptosis. Cardiovasc Res 105:8\u201319. https:\/\/doi.org\/10.1093\/cvr\/cvu241","journal-title":"Cardiovasc Res"},{"key":"2120_CR29","first-page":"235","volume":"23","author":"T Tian","year":"2017","unstructured":"Tian T, Li X, Hua Z, Ma J, Liu Z, Chen H, Cui Z (2017) S100A1 promotes cell proliferation and migration and is associated with lymph node metastasis in ovarian cancer. Discov Med 23:235\u2013245","journal-title":"Discov Med"},{"key":"2120_CR30","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1016\/j.celrep.2018.09.042","volume":"25","author":"J Wang","year":"2018","unstructured":"Wang J, Batourina E, Schneider K, Souza S, Swayne T, Liu C, George CD, Tate T, Dan H, Wiessner G, Zhuravlev Y, Canman JC, Mysorekar IU, Mendelsohn CL (2018) Polyploid superficial cells that maintain the urothelial barrier are produced via incomplete cytokinesis and endoreplication. Cell Rep 25:464\u2013477. https:\/\/doi.org\/10.1016\/j.celrep.2018.09.042","journal-title":"Cell Rep"},{"key":"2120_CR31","doi-asserted-by":"publisher","first-page":"5760","DOI":"10.7150\/jca.51855","volume":"12","author":"G Wang","year":"2021","unstructured":"Wang G, Li HN, Cui XQ, Xu T, Dong ML, Li SY, Li XR (2021) S100A1 is a potential biomarker for papillary thyroid carcinoma diagnosis and prognosis. J Cancer 12:5760\u20135771. https:\/\/doi.org\/10.7150\/jca.51855","journal-title":"J Cancer"},{"key":"2120_CR32","doi-asserted-by":"publisher","first-page":"5879","DOI":"10.3390\/ijms21165879","volume":"21","author":"J Weisz","year":"2020","unstructured":"Weisz J, Uversky VN (2020) Zooming into the dark side of human annexin-S100 complexes: dynamic alliance of flexible partners. Int J Mol Sci 21:5879. https:\/\/doi.org\/10.3390\/ijms21165879","journal-title":"Int J Mol Sci"},{"key":"2120_CR33","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.14670\/HH-22.1025","volume":"22","author":"R Yao","year":"2007","unstructured":"Yao R, Davidson DD, Lopez-Beltran A, MacLennan GT, Montironi R, Cheng L (2007a) The S100 proteins for screening and prognostic grading of bladder cancer. Histol Histopathol 22:1025\u20131032. https:\/\/doi.org\/10.14670\/HH-22.1025","journal-title":"Histol Histopathol"},{"key":"2120_CR34","first-page":"3051","volume":"27","author":"R Yao","year":"2007","unstructured":"Yao R, Lopez-Beltran A, Maclennan GT, Montiron IR, Eble JN, Cheng L (2007b) Expression of S100 protein family members in the pathogenesis of bladder tumors. Anticancer Res 27:3051\u20133058","journal-title":"Anticancer Res"},{"key":"2120_CR35","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/0361-9230(95)00040-2","volume":"37","author":"DB Zimmer","year":"1995","unstructured":"Zimmer DB, Cornwall EH, Landar A, Song W (1995) The S100 protein family: history, function, and expression. Brain Res Bull 37:417\u2013429. https:\/\/doi.org\/10.1016\/0361-9230(95)00040-2","journal-title":"Brain Res Bull"}],"container-title":["Histochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00418-022-02120-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00418-022-02120-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00418-022-02120-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T13:10:14Z","timestamp":1664197814000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00418-022-02120-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,1]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["2120"],"URL":"https:\/\/doi.org\/10.1007\/s00418-022-02120-1","relation":{},"ISSN":["0948-6143","1432-119X"],"issn-type":[{"value":"0948-6143","type":"print"},{"value":"1432-119X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,1]]},"assertion":[{"value":"18 May 2022","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 June 2022","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Animal experimental protocols were approved by the local Institutional Animal Care and Research Advisory Committee and permitted by the Lower Saxony State Office for Consumer Protection and Food Safety (AZ42500\/1H).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}