{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:09:52Z","timestamp":1779926992622,"version":"3.53.1"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"The Basic Research Program of Shanxi Province","award":["202203021222058"],"award-info":[{"award-number":["202203021222058"]}]},{"name":"The Basic Research Program of Shanxi Province","award":["202203021222058"],"award-info":[{"award-number":["202203021222058"]}]},{"name":"The Basic Research Program of Shanxi Province","award":["202203021222058"],"award-info":[{"award-number":["202203021222058"]}]},{"name":"The Dynamic Testing State Key Laboratory Fund","award":["2410600045MZ"],"award-info":[{"award-number":["2410600045MZ"]}]},{"name":"The Dynamic Testing State Key Laboratory Fund","award":["2410600045MZ"],"award-info":[{"award-number":["2410600045MZ"]}]},{"name":"The Dynamic Testing State Key Laboratory Fund","award":["2410600045MZ"],"award-info":[{"award-number":["2410600045MZ"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Histochem Cell Biol"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s00418-025-02393-2","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T10:47:05Z","timestamp":1749120425000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Quantitative analysis of cancer cell morphology using digital holography technology under high temperature stimulation"],"prefix":"10.1007","volume":"163","author":[{"given":"Zhuowen","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penglong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Zhen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanfei","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongmin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,5]]},"reference":[{"issue":"1","key":"2393_CR1","first-page":"52","volume":"1853","author":"B Averill","year":"2015","unstructured":"Averill B, Diana A, Bettaieb A (2015) Thermotolerance induced at a mild temperature of 40 degrees C alleviates heat shock-induced er stress and apoptosis in HeLa cells. Biochimica et biophysica acta. Mol Cell Res 1853(1):52\u201362","journal-title":"Mol Cell Res"},{"key":"2393_CR2","doi-asserted-by":"publisher","DOI":"10.5772\/55795","author":"A Bettaieb","year":"2013","unstructured":"Bettaieb A, Paulina K, Diana A (2013) Hyperthermia: cancer treatment and beyond. Semantic Scholar. https:\/\/doi.org\/10.5772\/55795","journal-title":"Semantic Scholar"},{"key":"2393_CR3","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.ceca.2018.04.004","volume":"73","author":"B Brett","year":"2018","unstructured":"Brett B, Yeranddy A, Alpizar et al (2018) Differential effects of lipopolysaccharide on mouse sensory TRP channels. Cell Calcium 73:72\u201381. https:\/\/doi.org\/10.1016\/j.ceca.2018.04.004","journal-title":"Cell Calcium"},{"issue":"1","key":"2393_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2105-14-297","volume":"14","author":"F Buggenthin","year":"2013","unstructured":"Buggenthin F, Marr C, Schwarzfischer M et al (2013) An automatic method for robust and fast cell detection in bright field images from high-throughput microscopy. BMC Bioinform 14(1):1\u201312. https:\/\/doi.org\/10.1186\/1471-2105-14-297","journal-title":"BMC Bioinform"},{"issue":"6","key":"2393_CR5","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1038\/s41592-024-02241-6","volume":"21","author":"SN Chandrasekaran","year":"2024","unstructured":"Chandrasekaran SN, Cimini BA, Goodale A et al (2024) Three million images and morphological profiles of cells treated with matched chemical and genetic perturbations. Nat Methods 21(6):25. https:\/\/doi.org\/10.1038\/s41592-024-02241-6","journal-title":"Nat Methods"},{"issue":"18","key":"2393_CR6","doi-asserted-by":"publisher","first-page":"11601","DOI":"10.1364\/OE.15.011601","volume":"15","author":"GL Chen","year":"2007","unstructured":"Chen GL, Lin CY, Yau HF et al (2007) Wavefront reconstruction without twin-image blurring by two arbitrary step digital holograms. Opt Express 15(18):11601. https:\/\/doi.org\/10.1364\/OE.15.011601","journal-title":"Opt Express"},{"issue":"48","key":"2393_CR7","doi-asserted-by":"publisher","first-page":"e2312603120","DOI":"10.1073\/pnas.2312603120","volume":"120","author":"X Chen","year":"2023","unstructured":"Chen X, Yang Q, Li YD (2023) High spatial-resolved heat manipulating membrane heterogeneity alters cellular migration and signaling. Proc Natl Acad Sci USA 120(48):e2312603120\u2013e2312603120. https:\/\/doi.org\/10.1073\/pnas.2312603120","journal-title":"Proc Natl Acad Sci USA"},{"issue":"4","key":"2393_CR8","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.pan.2024.05.012","volume":"42","author":"PD Chiaro","year":"2024","unstructured":"Chiaro PD, Nacci L, Arco F et al (2024) Mapping functional to morphological variation reveals the basis of regional extracellular matrix subversion and nerve invasion in pancreatic cancer. Cancer Cell 42(4):31. https:\/\/doi.org\/10.1016\/j.pan.2024.05.012","journal-title":"Cancer Cell"},{"issue":"31","key":"2393_CR9","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1063\/1.2808787","volume":"46","author":"PI Hariharan","year":"1993","unstructured":"Hariharan PI (1993) Basics of interferometry. Phys Today 46(31):75\u201375. https:\/\/doi.org\/10.1063\/1.2808787","journal-title":"Phys Today"},{"key":"2393_CR10","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-025-02647-w","author":"HC Hsieh","year":"2025","unstructured":"Hsieh HC, Han Q, Brenes D et al (2025) Imaging 3D cell cultures with optical microscopy. Nat Methods. https:\/\/doi.org\/10.1038\/s41592-025-02647-w","journal-title":"Nat Methods"},{"issue":"1","key":"2393_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1136\/jitc-2023-SITC2023.1290","volume":"11","author":"R Huang","year":"2023","unstructured":"Huang R, Mayer A, Trevino AE (2023) Quantitative cell morphology featurization in multiplexed immunofluorescence images reveals tumor subtypes in cancer microenvironments. J Immunother Cancer 11(1):1. https:\/\/doi.org\/10.1136\/jitc-2023-SITC2023.1290","journal-title":"J Immunother Cancer"},{"issue":"9","key":"2393_CR12","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1364\/OL.25.000610","volume":"25","author":"B Javidi","year":"2000","unstructured":"Javidi B, Tajahuerce E (2000) Three-dimensional object recognition by use of digital holography. Opt Lett 25(9):610\u2013612. https:\/\/doi.org\/10.1364\/OL.25.000610","journal-title":"Opt Lett"},{"key":"2393_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2024.110313","volume":"196","author":"O Jens","year":"2024","unstructured":"Jens O, Maarten CH, Olav D et al (2024) Hyperthermia as an adjuvant to radiation therapy in the treatment of locally advanced breast carcinoma. Radiother Oncol 196:110313. https:\/\/doi.org\/10.1016\/j.radonc.2024.110313","journal-title":"Radiother Oncol"},{"key":"2393_CR14","doi-asserted-by":"publisher","first-page":"7312","DOI":"10.1038\/s41467-024-51708-9","volume":"15","author":"J Jiang","year":"2024","unstructured":"Jiang J, Liu Y, Qin J et al (2024) METI: deep profiling of tumor ecosystems by integrating cell morphology and spatial transcriptomics. Nat Commun 15:7312. https:\/\/doi.org\/10.1038\/s41467-024-51708-9","journal-title":"Nat Commun"},{"key":"2393_CR15","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1038\/nphoton.2011.180","volume":"5","author":"M Kang","year":"2011","unstructured":"Kang M, Butsch A, Russell P (2011) Reconfigurable light-driven opto-acoustic isolators in photonic crystal fibre. Nature Photon 5:549\u2013553. https:\/\/doi.org\/10.1038\/nphoton.2011.180","journal-title":"Nature Photon"},{"key":"2393_CR16","doi-asserted-by":"publisher","DOI":"10.1117\/12.312966","author":"TM Kreis","year":"1998","unstructured":"Kreis TM, Jueptner WPO, Geldmacher J (1998) Principles of digital holographic interferometry. In Laser Interferometr IX: Tech Analysis. https:\/\/doi.org\/10.1117\/12.312966","journal-title":"In Laser Interferometr IX: Tech Analysis"},{"key":"2393_CR17","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/j.bbrc.2015.02.042","volume":"458","author":"G Kun","year":"2015","unstructured":"Kun G, Chen X, Xiaofeng J et al (2015) HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell growth in hepatocellular carcinoma. Biochem Biophys Res Commun 458:849\u2013855. https:\/\/doi.org\/10.1016\/j.bbrc.2015.02.042","journal-title":"Biochem Biophys Res Commun"},{"issue":"26","key":"2393_CR18","doi-asserted-by":"publisher","first-page":"6172","DOI":"10.1016\/j.biomaterials.2012.05.009","volume":"33","author":"ZY Lien","year":"2012","unstructured":"Lien ZY, Hsu TC, Liu KK et al (2012) Cancer cell labeling and tracking using fluorescent and magnetic nanodiamond. Biomaterials 33(26):6172\u20136185. https:\/\/doi.org\/10.1016\/j.biomaterials.2012.05.009","journal-title":"Biomaterials"},{"issue":"5","key":"2393_CR19","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1364\/OL.30.000468","volume":"30","author":"P Marquet","year":"2005","unstructured":"Marquet P, Rappaz B, Magistretti PJ et al (2005) Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. Opt Lett 30(5):468\u2013470. https:\/\/doi.org\/10.1364\/OL.30.000468","journal-title":"Opt Lett"},{"issue":"7","key":"2393_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041412","volume":"7","author":"Z Mianqiu","year":"2012","unstructured":"Mianqiu Z, Min J, Ye B, Hui Z, Zuomin Z et al (2012) Autophagy and apoptosis act as partners to induce germ cell death after heat stress in mice. PLoS ONE 7(7):e41412. https:\/\/doi.org\/10.1371\/journal.pone.0041412","journal-title":"PLoS ONE"},{"issue":"8","key":"2393_CR21","doi-asserted-by":"publisher","first-page":"1498","DOI":"10.1002\/anie.200805507","volume":"48","author":"A Neef","year":"2010","unstructured":"Neef A, Schultz C (2010) Selective fluorescence labeling of lipids in living cells. Angew Chem 48(8):1498\u20131500. https:\/\/doi.org\/10.1002\/anie.200805507","journal-title":"Angew Chem"},{"key":"2393_CR22","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1038\/sj.onc.1201210","volume":"15","author":"M Nitta","year":"1997","unstructured":"Nitta M, Okamura H, Aizawa S et al (1997) Heat shock induces transient p53-dependent cell cycle arrest at G1\/S. Oncogene 15:561\u2013568. https:\/\/doi.org\/10.1038\/sj.onc.1201210","journal-title":"Oncogene"},{"key":"2393_CR23","doi-asserted-by":"publisher","first-page":"7493","DOI":"10.1038\/s41467-022-35138-z","volume":"13","author":"K Oikawa","year":"2022","unstructured":"Oikawa K, Goto-Yamada S, Hayashi Y et al (2022) Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light. Nat Commun 13:7493. https:\/\/doi.org\/10.1038\/s41467-022-35138-z","journal-title":"Nat Commun"},{"issue":"4","key":"2393_CR24","first-page":"1","volume":"36","author":"CH Park","year":"2024","unstructured":"Park CH, Thompson IAP, Newman SS et al (2024) Real-time spatiotemporal measurement of extracellular signaling molecules using an aptamer switch-conjugated hydrogel matrix (adv. Mater. 4\/2024). Adv Mater 36(4):1","journal-title":"Adv Mater"},{"issue":"6","key":"2393_CR25","doi-asserted-by":"publisher","first-page":"733","DOI":"10.2174\/13862073113166660062","volume":"18","author":"B Rappaz","year":"2013","unstructured":"Rappaz B, Breton B, Shaffer E, Turcatti G (2013) Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening. Biomol Screening 18(6):733\u2013742. https:\/\/doi.org\/10.2174\/13862073113166660062","journal-title":"Biomol Screening"},{"key":"2393_CR26","doi-asserted-by":"publisher","DOI":"10.1007\/s00138-011-0337-9","author":"A Rehan","year":"2012","unstructured":"Rehan A, Mark G, T\u00fcnde S et al (2012) Automatic segmentation of adherent biological cell boundaries and nuclei from brightfield microscopy images. Mach vis Appl. https:\/\/doi.org\/10.1007\/s00138-011-0337-9","journal-title":"Mach vis Appl"},{"issue":"52","key":"2393_CR27","doi-asserted-by":"publisher","first-page":"20526","DOI":"10.1021\/acs.analchem.4c04872","volume":"96","author":"X Ren","year":"2024","unstructured":"Ren X, Zhang F, Gao G, Gao D et al (2024) Biophysical and morphological cell features retrieved by digital holographic microscopy correlate with drug-induced changes in cell migration behavior. Anal Chem 96(52):20526\u201320534. https:\/\/doi.org\/10.1021\/acs.analchem.4c04872","journal-title":"Anal Chem"},{"issue":"12","key":"2393_CR28","doi-asserted-by":"publisher","first-page":"1305","DOI":"10.1088\/0957-0233\/10\/12\/327","volume":"10","author":"FM Santoyo","year":"1999","unstructured":"Santoyo FM, Pedrini G, Schedin S et al (1999) 3D displacement measurements of vibrating objects with multi-pulse digital holography. Measurement Sci Technol 10(12):1305","journal-title":"Measurement Sci Technol"},{"issue":"5","key":"2393_CR29","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1002\/mpo.2950040415","volume":"25","author":"J Sirack\u00fd","year":"1978","unstructured":"Sirack\u00fd J, Schreiner P, Sirack\u00e1 E et al (1978) Cell proliferation kinetics and nuclear morphology in endometrial cancer under progesterone treatment. Neoplasma 25(5):535. https:\/\/doi.org\/10.1002\/mpo.2950040415","journal-title":"Neoplasma"},{"key":"2393_CR30","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202109349","author":"R Vilar","year":"2021","unstructured":"Vilar R, Priessner M, Summers PA et al (2021) Selective detection of Cu+ ions in live cells via fluorescence lifetime imaging microscopy. Angew Chem Int Ed. https:\/\/doi.org\/10.1002\/anie.202109349","journal-title":"Angew Chem Int Ed"},{"issue":"43","key":"2393_CR31","doi-asserted-by":"publisher","first-page":"48310","DOI":"10.1021\/acsami.0c13364","volume":"12","author":"J Wu","year":"2020","unstructured":"Wu J, Zhang S, Mei X et al (2020) Ultrathin transition metal chalcogenide nanosheets synthesized via topotactic transformation for effective cancer theranostics. ACS Appl Mater Interfaces 12(43):48310\u201348320. https:\/\/doi.org\/10.1021\/acsami.0c13364","journal-title":"ACS Appl Mater Interfaces"},{"key":"2393_CR32","doi-asserted-by":"publisher","DOI":"10.1364\/OL.23.001221","author":"T Zhang","year":"1998","unstructured":"Zhang T, Yamaguchi I (1998) Three-dimensional microscopy with phase-shifting digital holography. Opt Lett. https:\/\/doi.org\/10.1364\/OL.23.001221","journal-title":"Opt Lett"},{"key":"2393_CR33","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.737401","volume":"546","author":"X Zhao","year":"2022","unstructured":"Zhao X, Li L, Li C et al (2022) Heat stress-induced endoplasmic reticulum stress promotes liver apoptosis in largemouth bass (Micropterus salmoides). Aquaculture 546:737401. https:\/\/doi.org\/10.1016\/j.aquaculture.2021.737401","journal-title":"Aquaculture"},{"key":"2393_CR34","doi-asserted-by":"publisher","unstructured":"Zhao B, Liao W, Zhang H. (2009) Acquisition and processing of three-dimensional information by digital holography. 2009 International Conference on Information Engineering and Computer Science. https:\/\/doi.org\/10.1109\/ICIECS.2009.5362960","DOI":"10.1109\/ICIECS.2009.5362960"}],"container-title":["Histochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00418-025-02393-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00418-025-02393-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00418-025-02393-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T11:27:25Z","timestamp":1768390045000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00418-025-02393-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,5]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["2393"],"URL":"https:\/\/doi.org\/10.1007\/s00418-025-02393-2","relation":{},"ISSN":["0948-6143","1432-119X"],"issn-type":[{"value":"0948-6143","type":"print"},{"value":"1432-119X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,5]]},"assertion":[{"value":"19 May 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 June 2025","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that there are no financial interests, commercial affiliations, or other potential conflicts of interest that could have influenced the objectivity of this research or the writing of this paper","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"63"}}